Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster

Detalhes bibliográficos
Autor(a) principal: Ruiz, M. T.
Data de Publicação: 2003
Outros Autores: Carareto, CMA
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1034/j.1601-5223.2003.01708.x
http://hdl.handle.net/11449/34863
Resumo: We have determined the copy number and the presence of full-size hobo transposable elements in eight Brasilian strains of Drosophila melanogaster. Genomic DNA was digested with AvaII and XhoI restriction enzymes, respectively, and probed with a 963 bp sequence of the hobo element. Variable numbers of full-sized and defective elements were detected in all strains. The range of the copy number was 22.13 +/- 4.52. Blots showed the presence of a 2.6 kb fragment, corresponding to the complete element, in all strains exception of one and the 1.0 kb sequence, correponding to the Th1 and Th2 repressor elements. There was neither association among copy numbers of hobo elements and latitude nor the mean annual temperatures in the original geographical region of each strain.
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spelling Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogasterWe have determined the copy number and the presence of full-size hobo transposable elements in eight Brasilian strains of Drosophila melanogaster. Genomic DNA was digested with AvaII and XhoI restriction enzymes, respectively, and probed with a 963 bp sequence of the hobo element. Variable numbers of full-sized and defective elements were detected in all strains. The range of the copy number was 22.13 +/- 4.52. Blots showed the presence of a 2.6 kb fragment, corresponding to the complete element, in all strains exception of one and the 1.0 kb sequence, correponding to the Th1 and Th2 repressor elements. There was neither association among copy numbers of hobo elements and latitude nor the mean annual temperatures in the original geographical region of each strain.Univ Estadual Paulista, Dept Biol, UNESP, BR-15054000 Sao Jose do Rio Preto, SP, BrazilUniv Estadual Paulista, Dept Biol, UNESP, BR-15054000 Sao Jose do Rio Preto, SP, BrazilBlackwell MunksgaardUniversidade Estadual Paulista (Unesp)Ruiz, M. T.Carareto, CMA2014-05-20T15:24:13Z2014-05-20T15:24:13Z2003-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article154-157application/pdfhttp://dx.doi.org/10.1034/j.1601-5223.2003.01708.xHereditas. Copenhagen: Blackwell Munksgaard, v. 138, n. 2, p. 154-157, 2003.0018-0661http://hdl.handle.net/11449/3486310.1034/j.1601-5223.2003.01708.xWOS:000184609200010WOS000184609200010.pdf34257729983192160000-0002-0298-1354Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHereditas0,278info:eu-repo/semantics/openAccess2023-12-30T06:15:53Zoai:repositorio.unesp.br:11449/34863Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-30T06:15:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster
title Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster
spellingShingle Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster
Ruiz, M. T.
title_short Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster
title_full Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster
title_fullStr Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster
title_full_unstemmed Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster
title_sort Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster
author Ruiz, M. T.
author_facet Ruiz, M. T.
Carareto, CMA
author_role author
author2 Carareto, CMA
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ruiz, M. T.
Carareto, CMA
description We have determined the copy number and the presence of full-size hobo transposable elements in eight Brasilian strains of Drosophila melanogaster. Genomic DNA was digested with AvaII and XhoI restriction enzymes, respectively, and probed with a 963 bp sequence of the hobo element. Variable numbers of full-sized and defective elements were detected in all strains. The range of the copy number was 22.13 +/- 4.52. Blots showed the presence of a 2.6 kb fragment, corresponding to the complete element, in all strains exception of one and the 1.0 kb sequence, correponding to the Th1 and Th2 repressor elements. There was neither association among copy numbers of hobo elements and latitude nor the mean annual temperatures in the original geographical region of each strain.
publishDate 2003
dc.date.none.fl_str_mv 2003-01-01
2014-05-20T15:24:13Z
2014-05-20T15:24:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1034/j.1601-5223.2003.01708.x
Hereditas. Copenhagen: Blackwell Munksgaard, v. 138, n. 2, p. 154-157, 2003.
0018-0661
http://hdl.handle.net/11449/34863
10.1034/j.1601-5223.2003.01708.x
WOS:000184609200010
WOS000184609200010.pdf
3425772998319216
0000-0002-0298-1354
url http://dx.doi.org/10.1034/j.1601-5223.2003.01708.x
http://hdl.handle.net/11449/34863
identifier_str_mv Hereditas. Copenhagen: Blackwell Munksgaard, v. 138, n. 2, p. 154-157, 2003.
0018-0661
10.1034/j.1601-5223.2003.01708.x
WOS:000184609200010
WOS000184609200010.pdf
3425772998319216
0000-0002-0298-1354
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Hereditas
0,278
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 154-157
application/pdf
dc.publisher.none.fl_str_mv Blackwell Munksgaard
publisher.none.fl_str_mv Blackwell Munksgaard
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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