Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1034/j.1601-5223.2003.01708.x http://hdl.handle.net/11449/34863 |
Resumo: | We have determined the copy number and the presence of full-size hobo transposable elements in eight Brasilian strains of Drosophila melanogaster. Genomic DNA was digested with AvaII and XhoI restriction enzymes, respectively, and probed with a 963 bp sequence of the hobo element. Variable numbers of full-sized and defective elements were detected in all strains. The range of the copy number was 22.13 +/- 4.52. Blots showed the presence of a 2.6 kb fragment, corresponding to the complete element, in all strains exception of one and the 1.0 kb sequence, correponding to the Th1 and Th2 repressor elements. There was neither association among copy numbers of hobo elements and latitude nor the mean annual temperatures in the original geographical region of each strain. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogasterWe have determined the copy number and the presence of full-size hobo transposable elements in eight Brasilian strains of Drosophila melanogaster. Genomic DNA was digested with AvaII and XhoI restriction enzymes, respectively, and probed with a 963 bp sequence of the hobo element. Variable numbers of full-sized and defective elements were detected in all strains. The range of the copy number was 22.13 +/- 4.52. Blots showed the presence of a 2.6 kb fragment, corresponding to the complete element, in all strains exception of one and the 1.0 kb sequence, correponding to the Th1 and Th2 repressor elements. There was neither association among copy numbers of hobo elements and latitude nor the mean annual temperatures in the original geographical region of each strain.Univ Estadual Paulista, Dept Biol, UNESP, BR-15054000 Sao Jose do Rio Preto, SP, BrazilUniv Estadual Paulista, Dept Biol, UNESP, BR-15054000 Sao Jose do Rio Preto, SP, BrazilBlackwell MunksgaardUniversidade Estadual Paulista (Unesp)Ruiz, M. T.Carareto, CMA2014-05-20T15:24:13Z2014-05-20T15:24:13Z2003-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article154-157application/pdfhttp://dx.doi.org/10.1034/j.1601-5223.2003.01708.xHereditas. Copenhagen: Blackwell Munksgaard, v. 138, n. 2, p. 154-157, 2003.0018-0661http://hdl.handle.net/11449/3486310.1034/j.1601-5223.2003.01708.xWOS:000184609200010WOS000184609200010.pdf34257729983192160000-0002-0298-1354Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHereditas0,278info:eu-repo/semantics/openAccess2023-12-30T06:15:53Zoai:repositorio.unesp.br:11449/34863Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:39:34.335040Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster |
title |
Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster |
spellingShingle |
Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster Ruiz, M. T. |
title_short |
Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster |
title_full |
Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster |
title_fullStr |
Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster |
title_full_unstemmed |
Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster |
title_sort |
Characterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster |
author |
Ruiz, M. T. |
author_facet |
Ruiz, M. T. Carareto, CMA |
author_role |
author |
author2 |
Carareto, CMA |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ruiz, M. T. Carareto, CMA |
description |
We have determined the copy number and the presence of full-size hobo transposable elements in eight Brasilian strains of Drosophila melanogaster. Genomic DNA was digested with AvaII and XhoI restriction enzymes, respectively, and probed with a 963 bp sequence of the hobo element. Variable numbers of full-sized and defective elements were detected in all strains. The range of the copy number was 22.13 +/- 4.52. Blots showed the presence of a 2.6 kb fragment, corresponding to the complete element, in all strains exception of one and the 1.0 kb sequence, correponding to the Th1 and Th2 repressor elements. There was neither association among copy numbers of hobo elements and latitude nor the mean annual temperatures in the original geographical region of each strain. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-01-01 2014-05-20T15:24:13Z 2014-05-20T15:24:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1034/j.1601-5223.2003.01708.x Hereditas. Copenhagen: Blackwell Munksgaard, v. 138, n. 2, p. 154-157, 2003. 0018-0661 http://hdl.handle.net/11449/34863 10.1034/j.1601-5223.2003.01708.x WOS:000184609200010 WOS000184609200010.pdf 3425772998319216 0000-0002-0298-1354 |
url |
http://dx.doi.org/10.1034/j.1601-5223.2003.01708.x http://hdl.handle.net/11449/34863 |
identifier_str_mv |
Hereditas. Copenhagen: Blackwell Munksgaard, v. 138, n. 2, p. 154-157, 2003. 0018-0661 10.1034/j.1601-5223.2003.01708.x WOS:000184609200010 WOS000184609200010.pdf 3425772998319216 0000-0002-0298-1354 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Hereditas 0,278 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
154-157 application/pdf |
dc.publisher.none.fl_str_mv |
Blackwell Munksgaard |
publisher.none.fl_str_mv |
Blackwell Munksgaard |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129345251704832 |