Cytotoxic effects of Euterpe oleraceae fruit oil (acai) in rat liver and thyroid tissues
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bjp.2018.12.001 http://hdl.handle.net/11449/185518 |
Resumo: | Euterpe oleracea Mart., Arecaceae, fruit (acai) presents considerable potential for the development of new medicines due to its phytochemical composition and antioxidant activity. More recently, special attention has been given to the pharmacological potential of the fruit's oil. This study analysed the histological and histochemical effects of different dosages of acai oil on rat's liver and thyroid cells, in order to evaluate its cytotoxic potential after administration for consecutive days. Male Wistar rats were treated with the acai oil by gavage at doses of 30, 100 and 300 mg/kg, for 14 days, within a 24 h interval. Liver and thyroid fragments were collected for histology (hematoxylin and eosin) and histochemistry analysis (blue of Nilo (lipids), Baker (lipids), bromophenol blue (protein), PAS (polysaccharides)). The results showed that animals exposed to acai oil presented alterations in the liver cells, where the integrity of the liver tissue was increasingly lost as the acai oil doses increased. Nuclear pyknosis was observed in several hepatocytes, evidencing the occurrence of cell death. Alteration in the amount of lipids, polysaccharides, vacuoles in the cytoplasm, and proliferation of Kupffer cells were observed in histochemical analyzes. As for the thyroid of the treated rats, alterations were observed in the size of the follicular lumen and also in the connective tissue found between the follicles. Under the experimental conditions employed in the present study, the cytotoxicity observed in this work is worrying, specially considering the liver, when frequent or continuous damage could lead pathological disorders in this organ. (C) 2019 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. |
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Cytotoxic effects of Euterpe oleraceae fruit oil (acai) in rat liver and thyroid tissuesAcai oilThyroid cytotoxicityLiver cytotoxicityAcai cytotoxicityEuterpe oleracea Mart., Arecaceae, fruit (acai) presents considerable potential for the development of new medicines due to its phytochemical composition and antioxidant activity. More recently, special attention has been given to the pharmacological potential of the fruit's oil. This study analysed the histological and histochemical effects of different dosages of acai oil on rat's liver and thyroid cells, in order to evaluate its cytotoxic potential after administration for consecutive days. Male Wistar rats were treated with the acai oil by gavage at doses of 30, 100 and 300 mg/kg, for 14 days, within a 24 h interval. Liver and thyroid fragments were collected for histology (hematoxylin and eosin) and histochemistry analysis (blue of Nilo (lipids), Baker (lipids), bromophenol blue (protein), PAS (polysaccharides)). The results showed that animals exposed to acai oil presented alterations in the liver cells, where the integrity of the liver tissue was increasingly lost as the acai oil doses increased. Nuclear pyknosis was observed in several hepatocytes, evidencing the occurrence of cell death. Alteration in the amount of lipids, polysaccharides, vacuoles in the cytoplasm, and proliferation of Kupffer cells were observed in histochemical analyzes. As for the thyroid of the treated rats, alterations were observed in the size of the follicular lumen and also in the connective tissue found between the follicles. Under the experimental conditions employed in the present study, the cytotoxicity observed in this work is worrying, specially considering the liver, when frequent or continuous damage could lead pathological disorders in this organ. (C) 2019 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Programa Posgrad Biol Geral & Aplicada, Inst Biociencias, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Biol, Inst Biociencias, Rio Claro, SP, BrazilUniv Fed Sao Paulo, Dept Ciencias Exatas & Terra, Diadema, SP, BrazilUniv Estadual Campinas, Fac Ciencias Med, Campinas, SP, BrazilUniv Tras os Montes & Alto Douro, Escola Ciencias Agr & Vet, Vila Real, PortugalUniv Tras os Montes & Alto Douro, Dept Genet & Biotecnol, Vila Real, PortugalUniv Estadual Paulista, Dept Fonoaudiol, Fac Filosofia & Ciencias, Marilia, SP, BrazilUniv Estadual Paulista, Programa Posgrad Biol Geral & Aplicada, Inst Biociencias, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Biol, Inst Biociencias, Rio Claro, SP, BrazilUniv Estadual Paulista, Dept Fonoaudiol, Fac Filosofia & Ciencias, Marilia, SP, BrazilFAPESP: 2012/17241-8Soc Brasileira FarmacognosiaUniversidade Estadual Paulista (Unesp)Universidade Federal de São Paulo (UNIFESP)Universidade Estadual de Campinas (UNICAMP)Univ Tras os Montes & Alto DouroMarques, Eduardo de Souza [UNESP]Froder, Juliano Gabriel [UNESP]Oliveira, Patricia Rosa de [UNESP]Perazzo, Fabio FerreiraPires Rosa, Paulo CesarMascarenhas Gaivao, Isabel O'Neill deCamargo Mathias, Maria Izabel [UNESP]Maistro, Edson Luis [UNESP]2019-10-04T12:36:05Z2019-10-04T12:36:05Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article54-61application/pdfhttp://dx.doi.org/10.1016/j.bjp.2018.12.001Revista Brasileira De Farmacognosia-brazilian Journal Of Pharmacognosy. Curitiba-pr: Soc Brasileira Farmacognosia, v. 29, n. 1, p. 54-61, 2019.0102-695Xhttp://hdl.handle.net/11449/18551810.1016/j.bjp.2018.12.001S0102-695X2019000100054WOS:000460752000009S0102-695X2019000100054.pdf47875216130383150000-0003-0757-7876Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira De Farmacognosia-brazilian Journal Of Pharmacognosyinfo:eu-repo/semantics/openAccess2023-11-15T06:12:58Zoai:repositorio.unesp.br:11449/185518Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-15T06:12:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytotoxic effects of Euterpe oleraceae fruit oil (acai) in rat liver and thyroid tissues |
title |
Cytotoxic effects of Euterpe oleraceae fruit oil (acai) in rat liver and thyroid tissues |
spellingShingle |
Cytotoxic effects of Euterpe oleraceae fruit oil (acai) in rat liver and thyroid tissues Marques, Eduardo de Souza [UNESP] Acai oil Thyroid cytotoxicity Liver cytotoxicity Acai cytotoxicity |
title_short |
Cytotoxic effects of Euterpe oleraceae fruit oil (acai) in rat liver and thyroid tissues |
title_full |
Cytotoxic effects of Euterpe oleraceae fruit oil (acai) in rat liver and thyroid tissues |
title_fullStr |
Cytotoxic effects of Euterpe oleraceae fruit oil (acai) in rat liver and thyroid tissues |
title_full_unstemmed |
Cytotoxic effects of Euterpe oleraceae fruit oil (acai) in rat liver and thyroid tissues |
title_sort |
Cytotoxic effects of Euterpe oleraceae fruit oil (acai) in rat liver and thyroid tissues |
author |
Marques, Eduardo de Souza [UNESP] |
author_facet |
Marques, Eduardo de Souza [UNESP] Froder, Juliano Gabriel [UNESP] Oliveira, Patricia Rosa de [UNESP] Perazzo, Fabio Ferreira Pires Rosa, Paulo Cesar Mascarenhas Gaivao, Isabel O'Neill de Camargo Mathias, Maria Izabel [UNESP] Maistro, Edson Luis [UNESP] |
author_role |
author |
author2 |
Froder, Juliano Gabriel [UNESP] Oliveira, Patricia Rosa de [UNESP] Perazzo, Fabio Ferreira Pires Rosa, Paulo Cesar Mascarenhas Gaivao, Isabel O'Neill de Camargo Mathias, Maria Izabel [UNESP] Maistro, Edson Luis [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Paulo (UNIFESP) Universidade Estadual de Campinas (UNICAMP) Univ Tras os Montes & Alto Douro |
dc.contributor.author.fl_str_mv |
Marques, Eduardo de Souza [UNESP] Froder, Juliano Gabriel [UNESP] Oliveira, Patricia Rosa de [UNESP] Perazzo, Fabio Ferreira Pires Rosa, Paulo Cesar Mascarenhas Gaivao, Isabel O'Neill de Camargo Mathias, Maria Izabel [UNESP] Maistro, Edson Luis [UNESP] |
dc.subject.por.fl_str_mv |
Acai oil Thyroid cytotoxicity Liver cytotoxicity Acai cytotoxicity |
topic |
Acai oil Thyroid cytotoxicity Liver cytotoxicity Acai cytotoxicity |
description |
Euterpe oleracea Mart., Arecaceae, fruit (acai) presents considerable potential for the development of new medicines due to its phytochemical composition and antioxidant activity. More recently, special attention has been given to the pharmacological potential of the fruit's oil. This study analysed the histological and histochemical effects of different dosages of acai oil on rat's liver and thyroid cells, in order to evaluate its cytotoxic potential after administration for consecutive days. Male Wistar rats were treated with the acai oil by gavage at doses of 30, 100 and 300 mg/kg, for 14 days, within a 24 h interval. Liver and thyroid fragments were collected for histology (hematoxylin and eosin) and histochemistry analysis (blue of Nilo (lipids), Baker (lipids), bromophenol blue (protein), PAS (polysaccharides)). The results showed that animals exposed to acai oil presented alterations in the liver cells, where the integrity of the liver tissue was increasingly lost as the acai oil doses increased. Nuclear pyknosis was observed in several hepatocytes, evidencing the occurrence of cell death. Alteration in the amount of lipids, polysaccharides, vacuoles in the cytoplasm, and proliferation of Kupffer cells were observed in histochemical analyzes. As for the thyroid of the treated rats, alterations were observed in the size of the follicular lumen and also in the connective tissue found between the follicles. Under the experimental conditions employed in the present study, the cytotoxicity observed in this work is worrying, specially considering the liver, when frequent or continuous damage could lead pathological disorders in this organ. (C) 2019 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:36:05Z 2019-10-04T12:36:05Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bjp.2018.12.001 Revista Brasileira De Farmacognosia-brazilian Journal Of Pharmacognosy. Curitiba-pr: Soc Brasileira Farmacognosia, v. 29, n. 1, p. 54-61, 2019. 0102-695X http://hdl.handle.net/11449/185518 10.1016/j.bjp.2018.12.001 S0102-695X2019000100054 WOS:000460752000009 S0102-695X2019000100054.pdf 4787521613038315 0000-0003-0757-7876 |
url |
http://dx.doi.org/10.1016/j.bjp.2018.12.001 http://hdl.handle.net/11449/185518 |
identifier_str_mv |
Revista Brasileira De Farmacognosia-brazilian Journal Of Pharmacognosy. Curitiba-pr: Soc Brasileira Farmacognosia, v. 29, n. 1, p. 54-61, 2019. 0102-695X 10.1016/j.bjp.2018.12.001 S0102-695X2019000100054 WOS:000460752000009 S0102-695X2019000100054.pdf 4787521613038315 0000-0003-0757-7876 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira De Farmacognosia-brazilian Journal Of Pharmacognosy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
54-61 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasileira Farmacognosia |
publisher.none.fl_str_mv |
Soc Brasileira Farmacognosia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964942372175872 |