The functional activity of the miR-1914-5p in lipid metabolism of the hepatocarcinoma cell line HepG2: a potential molecular tool for controlling hepatic cellular migration
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11033-021-06364-3 http://hdl.handle.net/11449/210278 |
Resumo: | Hepatocellular carcinoma is one of the most common types of cancer in the world with high mortality rate and new therapies that control of fatty acid metabolism may limit the proliferation of cancer cells. In the last two decades, the non-coding RNAs have been considered as promising molecular tools to treat diseases, because they are able to modulate gene expression and the metabolic routes; however, deep investigation of their mechanistic behavior in pathologies must be performed. Thus, our aim was to evaluate the modulatory effect of the miR-1914-5p in controlling lipid metabolism in HepG2, a widely used human hepatocarcinoma cell line. The molecular and cellular analyses demonstrated that the functional inhibition of the investigated microRNA completely changed the cellular metabolism and behavior, compared to control groups. The in vitro inhibition of the miR-1914-5p increased the energy expenditure pointed in different analyses, decreasing cell doubling time and migration rate verified in wound healing and in the classical transwell chambers invasion assays, which makes the miR-1914-5p a candidate for further translational and preclinical studies to validate its function in controlling metastasis in liver cancer or even treat those diseases. |
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The functional activity of the miR-1914-5p in lipid metabolism of the hepatocarcinoma cell line HepG2: a potential molecular tool for controlling hepatic cellular migrationCell migrationCellular mechanismsHepatic diseaseLipid metabolismNon-coding RNAsSystems biologyHepatocellular carcinoma is one of the most common types of cancer in the world with high mortality rate and new therapies that control of fatty acid metabolism may limit the proliferation of cancer cells. In the last two decades, the non-coding RNAs have been considered as promising molecular tools to treat diseases, because they are able to modulate gene expression and the metabolic routes; however, deep investigation of their mechanistic behavior in pathologies must be performed. Thus, our aim was to evaluate the modulatory effect of the miR-1914-5p in controlling lipid metabolism in HepG2, a widely used human hepatocarcinoma cell line. The molecular and cellular analyses demonstrated that the functional inhibition of the investigated microRNA completely changed the cellular metabolism and behavior, compared to control groups. The in vitro inhibition of the miR-1914-5p increased the energy expenditure pointed in different analyses, decreasing cell doubling time and migration rate verified in wound healing and in the classical transwell chambers invasion assays, which makes the miR-1914-5p a candidate for further translational and preclinical studies to validate its function in controlling metastasis in liver cancer or even treat those diseases.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Dept Biol Geral & Aplicada, Inst Biociencias, Lab Biol Mol, Campus Rio Claro, BR-13506900 Rio Claro, SP, BrazilUniv Estadual Paulista, Ctr Estudos Insetos Sociais, Inst Biociencia, Rio Claro, SP, BrazilUniv Estadual Paulista, Dept Biol Geral & Aplicada, Inst Biociencias, Lab Biol Mol, Campus Rio Claro, BR-13506900 Rio Claro, SP, BrazilUniv Estadual Paulista, Ctr Estudos Insetos Sociais, Inst Biociencia, Rio Claro, SP, BrazilFAPESP: FAPESP-2013/21186-5FAPESP: 2018/05286-3SpringerUniversidade Estadual Paulista (Unesp)Silveira, Marina Bonfogo da [UNESP]Pansa, Camila Cristiane [UNESP]Malaspina, Osmar [UNESP]Moraes, Karen C. M. [UNESP]2021-06-25T15:03:31Z2021-06-25T15:03:31Z2021-04-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.1007/s11033-021-06364-3Molecular Biology Reports. Dordrecht: Springer, 12 p., 2021.0301-4851http://hdl.handle.net/11449/21027810.1007/s11033-021-06364-3WOS:000644750500001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular Biology Reportsinfo:eu-repo/semantics/openAccess2021-10-23T20:17:26Zoai:repositorio.unesp.br:11449/210278Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:09:19.475386Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The functional activity of the miR-1914-5p in lipid metabolism of the hepatocarcinoma cell line HepG2: a potential molecular tool for controlling hepatic cellular migration |
title |
The functional activity of the miR-1914-5p in lipid metabolism of the hepatocarcinoma cell line HepG2: a potential molecular tool for controlling hepatic cellular migration |
spellingShingle |
The functional activity of the miR-1914-5p in lipid metabolism of the hepatocarcinoma cell line HepG2: a potential molecular tool for controlling hepatic cellular migration Silveira, Marina Bonfogo da [UNESP] Cell migration Cellular mechanisms Hepatic disease Lipid metabolism Non-coding RNAs Systems biology |
title_short |
The functional activity of the miR-1914-5p in lipid metabolism of the hepatocarcinoma cell line HepG2: a potential molecular tool for controlling hepatic cellular migration |
title_full |
The functional activity of the miR-1914-5p in lipid metabolism of the hepatocarcinoma cell line HepG2: a potential molecular tool for controlling hepatic cellular migration |
title_fullStr |
The functional activity of the miR-1914-5p in lipid metabolism of the hepatocarcinoma cell line HepG2: a potential molecular tool for controlling hepatic cellular migration |
title_full_unstemmed |
The functional activity of the miR-1914-5p in lipid metabolism of the hepatocarcinoma cell line HepG2: a potential molecular tool for controlling hepatic cellular migration |
title_sort |
The functional activity of the miR-1914-5p in lipid metabolism of the hepatocarcinoma cell line HepG2: a potential molecular tool for controlling hepatic cellular migration |
author |
Silveira, Marina Bonfogo da [UNESP] |
author_facet |
Silveira, Marina Bonfogo da [UNESP] Pansa, Camila Cristiane [UNESP] Malaspina, Osmar [UNESP] Moraes, Karen C. M. [UNESP] |
author_role |
author |
author2 |
Pansa, Camila Cristiane [UNESP] Malaspina, Osmar [UNESP] Moraes, Karen C. M. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silveira, Marina Bonfogo da [UNESP] Pansa, Camila Cristiane [UNESP] Malaspina, Osmar [UNESP] Moraes, Karen C. M. [UNESP] |
dc.subject.por.fl_str_mv |
Cell migration Cellular mechanisms Hepatic disease Lipid metabolism Non-coding RNAs Systems biology |
topic |
Cell migration Cellular mechanisms Hepatic disease Lipid metabolism Non-coding RNAs Systems biology |
description |
Hepatocellular carcinoma is one of the most common types of cancer in the world with high mortality rate and new therapies that control of fatty acid metabolism may limit the proliferation of cancer cells. In the last two decades, the non-coding RNAs have been considered as promising molecular tools to treat diseases, because they are able to modulate gene expression and the metabolic routes; however, deep investigation of their mechanistic behavior in pathologies must be performed. Thus, our aim was to evaluate the modulatory effect of the miR-1914-5p in controlling lipid metabolism in HepG2, a widely used human hepatocarcinoma cell line. The molecular and cellular analyses demonstrated that the functional inhibition of the investigated microRNA completely changed the cellular metabolism and behavior, compared to control groups. The in vitro inhibition of the miR-1914-5p increased the energy expenditure pointed in different analyses, decreasing cell doubling time and migration rate verified in wound healing and in the classical transwell chambers invasion assays, which makes the miR-1914-5p a candidate for further translational and preclinical studies to validate its function in controlling metastasis in liver cancer or even treat those diseases. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T15:03:31Z 2021-06-25T15:03:31Z 2021-04-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11033-021-06364-3 Molecular Biology Reports. Dordrecht: Springer, 12 p., 2021. 0301-4851 http://hdl.handle.net/11449/210278 10.1007/s11033-021-06364-3 WOS:000644750500001 |
url |
http://dx.doi.org/10.1007/s11033-021-06364-3 http://hdl.handle.net/11449/210278 |
identifier_str_mv |
Molecular Biology Reports. Dordrecht: Springer, 12 p., 2021. 0301-4851 10.1007/s11033-021-06364-3 WOS:000644750500001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecular Biology Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128323199434752 |