Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study

Detalhes bibliográficos
Autor(a) principal: Aranha, Maria Luiza Garcia
Data de Publicação: 2021
Outros Autores: Garcia, Mariana Simões, de Carvalho Cavalcante, Deborah Navit, Silva, Ana Priscila Gomes, Fontes, Mayana Karoline [UNESP], Gusso-Choueri, Paloma Kachel [UNESP], Choueri, Rodrigo Brasil, Perobelli, Juliana Elaine
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.tox.2020.152636
http://hdl.handle.net/11449/206932
Resumo: The widespread use of agrochemicals results in the exposure of the general human population, including children, to several of these chemicals simultaneously. In the present preclinical study, it was investigated the hepatic damages caused by exposure to acephate, carbendazim and mancozeb when administered alone or in different combinations (binary and ternary). Juvenile male Wistar rats were exposed to agrochemicals from post-natal day 53, by gavage. The doses of agrochemicals applied here were determined from previous studies whose results showed no signs of systemic toxicity. All exposures provoked a significant increase in DNA damage (except for acephate alone) and activation of the xenobiotic biotransformation system (except for the ternary mixture). Interestingly, the ternary mixture did not exhibit an exacerbation in adverse effects caused by agrochemicals isolated or in binary combination, even though they are sharing genotoxicity damage induction as a common toxicity pathway. Conversely, some effects observed for isolated or binary combinations of agrochemicals were not observed for ternary combination, suggesting a chemical interaction that could imply antagonism character. Using a multivariate data analysis approach, exposure to isolated agrochemicals were related to a group of adverse effects characterized by hepatic lesion and the attempt of the tissue to mobilize defense cells and increase mitotic rates to minimize damages. Binary mixtures also share similarities in relation to the effects they exhibited, mainly a moderate to high increase in the GST activity and in histopathological alterations suggesting that binary combinations trigger an increased response of the mechanism of xenobiotics biotransformation. Together, obtained results bring important insights regarding adverse effects and possible interaction of the three agrochemicals whose residues are commonly detected in agro-food products.
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spelling Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis studyBiochemical biomarkersHistopathologyMixturesOxidative stressPesticidesThe widespread use of agrochemicals results in the exposure of the general human population, including children, to several of these chemicals simultaneously. In the present preclinical study, it was investigated the hepatic damages caused by exposure to acephate, carbendazim and mancozeb when administered alone or in different combinations (binary and ternary). Juvenile male Wistar rats were exposed to agrochemicals from post-natal day 53, by gavage. The doses of agrochemicals applied here were determined from previous studies whose results showed no signs of systemic toxicity. All exposures provoked a significant increase in DNA damage (except for acephate alone) and activation of the xenobiotic biotransformation system (except for the ternary mixture). Interestingly, the ternary mixture did not exhibit an exacerbation in adverse effects caused by agrochemicals isolated or in binary combination, even though they are sharing genotoxicity damage induction as a common toxicity pathway. Conversely, some effects observed for isolated or binary combinations of agrochemicals were not observed for ternary combination, suggesting a chemical interaction that could imply antagonism character. Using a multivariate data analysis approach, exposure to isolated agrochemicals were related to a group of adverse effects characterized by hepatic lesion and the attempt of the tissue to mobilize defense cells and increase mitotic rates to minimize damages. Binary mixtures also share similarities in relation to the effects they exhibited, mainly a moderate to high increase in the GST activity and in histopathological alterations suggesting that binary combinations trigger an increased response of the mechanism of xenobiotics biotransformation. Together, obtained results bring important insights regarding adverse effects and possible interaction of the three agrochemicals whose residues are commonly detected in agro-food products.Experimental Toxicology Laboratory Departamento de Ciências do Mar Universidade Federal de São Paulo – UNIFESPNúcleo de Estudos em Poluição e Ecotoxicologia Aquática Campus do Litoral Paulista Universidade Estadual Paulista – UNESPDepartamento de Ciências do Mar Universidade Federal de São Paulo – UNIFESPNúcleo de Estudos em Poluição e Ecotoxicologia Aquática Campus do Litoral Paulista Universidade Estadual Paulista – UNESPUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)Aranha, Maria Luiza GarciaGarcia, Mariana Simõesde Carvalho Cavalcante, Deborah NavitSilva, Ana Priscila GomesFontes, Mayana Karoline [UNESP]Gusso-Choueri, Paloma Kachel [UNESP]Choueri, Rodrigo BrasilPerobelli, Juliana Elaine2021-06-25T10:46:14Z2021-06-25T10:46:14Z2021-01-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.tox.2020.152636Toxicology, v. 447.1879-31850300-483Xhttp://hdl.handle.net/11449/20693210.1016/j.tox.2020.1526362-s2.0-85097217244Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicologyinfo:eu-repo/semantics/openAccess2021-10-23T15:41:46Zoai:repositorio.unesp.br:11449/206932Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T15:41:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study
title Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study
spellingShingle Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study
Aranha, Maria Luiza Garcia
Biochemical biomarkers
Histopathology
Mixtures
Oxidative stress
Pesticides
title_short Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study
title_full Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study
title_fullStr Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study
title_full_unstemmed Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study
title_sort Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study
author Aranha, Maria Luiza Garcia
author_facet Aranha, Maria Luiza Garcia
Garcia, Mariana Simões
de Carvalho Cavalcante, Deborah Navit
Silva, Ana Priscila Gomes
Fontes, Mayana Karoline [UNESP]
Gusso-Choueri, Paloma Kachel [UNESP]
Choueri, Rodrigo Brasil
Perobelli, Juliana Elaine
author_role author
author2 Garcia, Mariana Simões
de Carvalho Cavalcante, Deborah Navit
Silva, Ana Priscila Gomes
Fontes, Mayana Karoline [UNESP]
Gusso-Choueri, Paloma Kachel [UNESP]
Choueri, Rodrigo Brasil
Perobelli, Juliana Elaine
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Aranha, Maria Luiza Garcia
Garcia, Mariana Simões
de Carvalho Cavalcante, Deborah Navit
Silva, Ana Priscila Gomes
Fontes, Mayana Karoline [UNESP]
Gusso-Choueri, Paloma Kachel [UNESP]
Choueri, Rodrigo Brasil
Perobelli, Juliana Elaine
dc.subject.por.fl_str_mv Biochemical biomarkers
Histopathology
Mixtures
Oxidative stress
Pesticides
topic Biochemical biomarkers
Histopathology
Mixtures
Oxidative stress
Pesticides
description The widespread use of agrochemicals results in the exposure of the general human population, including children, to several of these chemicals simultaneously. In the present preclinical study, it was investigated the hepatic damages caused by exposure to acephate, carbendazim and mancozeb when administered alone or in different combinations (binary and ternary). Juvenile male Wistar rats were exposed to agrochemicals from post-natal day 53, by gavage. The doses of agrochemicals applied here were determined from previous studies whose results showed no signs of systemic toxicity. All exposures provoked a significant increase in DNA damage (except for acephate alone) and activation of the xenobiotic biotransformation system (except for the ternary mixture). Interestingly, the ternary mixture did not exhibit an exacerbation in adverse effects caused by agrochemicals isolated or in binary combination, even though they are sharing genotoxicity damage induction as a common toxicity pathway. Conversely, some effects observed for isolated or binary combinations of agrochemicals were not observed for ternary combination, suggesting a chemical interaction that could imply antagonism character. Using a multivariate data analysis approach, exposure to isolated agrochemicals were related to a group of adverse effects characterized by hepatic lesion and the attempt of the tissue to mobilize defense cells and increase mitotic rates to minimize damages. Binary mixtures also share similarities in relation to the effects they exhibited, mainly a moderate to high increase in the GST activity and in histopathological alterations suggesting that binary combinations trigger an increased response of the mechanism of xenobiotics biotransformation. Together, obtained results bring important insights regarding adverse effects and possible interaction of the three agrochemicals whose residues are commonly detected in agro-food products.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:46:14Z
2021-06-25T10:46:14Z
2021-01-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.tox.2020.152636
Toxicology, v. 447.
1879-3185
0300-483X
http://hdl.handle.net/11449/206932
10.1016/j.tox.2020.152636
2-s2.0-85097217244
url http://dx.doi.org/10.1016/j.tox.2020.152636
http://hdl.handle.net/11449/206932
identifier_str_mv Toxicology, v. 447.
1879-3185
0300-483X
10.1016/j.tox.2020.152636
2-s2.0-85097217244
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxicology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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