Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.tox.2020.152636 http://hdl.handle.net/11449/206932 |
Resumo: | The widespread use of agrochemicals results in the exposure of the general human population, including children, to several of these chemicals simultaneously. In the present preclinical study, it was investigated the hepatic damages caused by exposure to acephate, carbendazim and mancozeb when administered alone or in different combinations (binary and ternary). Juvenile male Wistar rats were exposed to agrochemicals from post-natal day 53, by gavage. The doses of agrochemicals applied here were determined from previous studies whose results showed no signs of systemic toxicity. All exposures provoked a significant increase in DNA damage (except for acephate alone) and activation of the xenobiotic biotransformation system (except for the ternary mixture). Interestingly, the ternary mixture did not exhibit an exacerbation in adverse effects caused by agrochemicals isolated or in binary combination, even though they are sharing genotoxicity damage induction as a common toxicity pathway. Conversely, some effects observed for isolated or binary combinations of agrochemicals were not observed for ternary combination, suggesting a chemical interaction that could imply antagonism character. Using a multivariate data analysis approach, exposure to isolated agrochemicals were related to a group of adverse effects characterized by hepatic lesion and the attempt of the tissue to mobilize defense cells and increase mitotic rates to minimize damages. Binary mixtures also share similarities in relation to the effects they exhibited, mainly a moderate to high increase in the GST activity and in histopathological alterations suggesting that binary combinations trigger an increased response of the mechanism of xenobiotics biotransformation. Together, obtained results bring important insights regarding adverse effects and possible interaction of the three agrochemicals whose residues are commonly detected in agro-food products. |
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Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis studyBiochemical biomarkersHistopathologyMixturesOxidative stressPesticidesThe widespread use of agrochemicals results in the exposure of the general human population, including children, to several of these chemicals simultaneously. In the present preclinical study, it was investigated the hepatic damages caused by exposure to acephate, carbendazim and mancozeb when administered alone or in different combinations (binary and ternary). Juvenile male Wistar rats were exposed to agrochemicals from post-natal day 53, by gavage. The doses of agrochemicals applied here were determined from previous studies whose results showed no signs of systemic toxicity. All exposures provoked a significant increase in DNA damage (except for acephate alone) and activation of the xenobiotic biotransformation system (except for the ternary mixture). Interestingly, the ternary mixture did not exhibit an exacerbation in adverse effects caused by agrochemicals isolated or in binary combination, even though they are sharing genotoxicity damage induction as a common toxicity pathway. Conversely, some effects observed for isolated or binary combinations of agrochemicals were not observed for ternary combination, suggesting a chemical interaction that could imply antagonism character. Using a multivariate data analysis approach, exposure to isolated agrochemicals were related to a group of adverse effects characterized by hepatic lesion and the attempt of the tissue to mobilize defense cells and increase mitotic rates to minimize damages. Binary mixtures also share similarities in relation to the effects they exhibited, mainly a moderate to high increase in the GST activity and in histopathological alterations suggesting that binary combinations trigger an increased response of the mechanism of xenobiotics biotransformation. Together, obtained results bring important insights regarding adverse effects and possible interaction of the three agrochemicals whose residues are commonly detected in agro-food products.Experimental Toxicology Laboratory Departamento de Ciências do Mar Universidade Federal de São Paulo – UNIFESPNúcleo de Estudos em Poluição e Ecotoxicologia Aquática Campus do Litoral Paulista Universidade Estadual Paulista – UNESPDepartamento de Ciências do Mar Universidade Federal de São Paulo – UNIFESPNúcleo de Estudos em Poluição e Ecotoxicologia Aquática Campus do Litoral Paulista Universidade Estadual Paulista – UNESPUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)Aranha, Maria Luiza GarciaGarcia, Mariana Simõesde Carvalho Cavalcante, Deborah NavitSilva, Ana Priscila GomesFontes, Mayana Karoline [UNESP]Gusso-Choueri, Paloma Kachel [UNESP]Choueri, Rodrigo BrasilPerobelli, Juliana Elaine2021-06-25T10:46:14Z2021-06-25T10:46:14Z2021-01-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.tox.2020.152636Toxicology, v. 447.1879-31850300-483Xhttp://hdl.handle.net/11449/20693210.1016/j.tox.2020.1526362-s2.0-85097217244Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicologyinfo:eu-repo/semantics/openAccess2021-10-23T15:41:46Zoai:repositorio.unesp.br:11449/206932Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T15:41:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study |
title |
Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study |
spellingShingle |
Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study Aranha, Maria Luiza Garcia Biochemical biomarkers Histopathology Mixtures Oxidative stress Pesticides |
title_short |
Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study |
title_full |
Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study |
title_fullStr |
Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study |
title_full_unstemmed |
Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study |
title_sort |
Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study |
author |
Aranha, Maria Luiza Garcia |
author_facet |
Aranha, Maria Luiza Garcia Garcia, Mariana Simões de Carvalho Cavalcante, Deborah Navit Silva, Ana Priscila Gomes Fontes, Mayana Karoline [UNESP] Gusso-Choueri, Paloma Kachel [UNESP] Choueri, Rodrigo Brasil Perobelli, Juliana Elaine |
author_role |
author |
author2 |
Garcia, Mariana Simões de Carvalho Cavalcante, Deborah Navit Silva, Ana Priscila Gomes Fontes, Mayana Karoline [UNESP] Gusso-Choueri, Paloma Kachel [UNESP] Choueri, Rodrigo Brasil Perobelli, Juliana Elaine |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Aranha, Maria Luiza Garcia Garcia, Mariana Simões de Carvalho Cavalcante, Deborah Navit Silva, Ana Priscila Gomes Fontes, Mayana Karoline [UNESP] Gusso-Choueri, Paloma Kachel [UNESP] Choueri, Rodrigo Brasil Perobelli, Juliana Elaine |
dc.subject.por.fl_str_mv |
Biochemical biomarkers Histopathology Mixtures Oxidative stress Pesticides |
topic |
Biochemical biomarkers Histopathology Mixtures Oxidative stress Pesticides |
description |
The widespread use of agrochemicals results in the exposure of the general human population, including children, to several of these chemicals simultaneously. In the present preclinical study, it was investigated the hepatic damages caused by exposure to acephate, carbendazim and mancozeb when administered alone or in different combinations (binary and ternary). Juvenile male Wistar rats were exposed to agrochemicals from post-natal day 53, by gavage. The doses of agrochemicals applied here were determined from previous studies whose results showed no signs of systemic toxicity. All exposures provoked a significant increase in DNA damage (except for acephate alone) and activation of the xenobiotic biotransformation system (except for the ternary mixture). Interestingly, the ternary mixture did not exhibit an exacerbation in adverse effects caused by agrochemicals isolated or in binary combination, even though they are sharing genotoxicity damage induction as a common toxicity pathway. Conversely, some effects observed for isolated or binary combinations of agrochemicals were not observed for ternary combination, suggesting a chemical interaction that could imply antagonism character. Using a multivariate data analysis approach, exposure to isolated agrochemicals were related to a group of adverse effects characterized by hepatic lesion and the attempt of the tissue to mobilize defense cells and increase mitotic rates to minimize damages. Binary mixtures also share similarities in relation to the effects they exhibited, mainly a moderate to high increase in the GST activity and in histopathological alterations suggesting that binary combinations trigger an increased response of the mechanism of xenobiotics biotransformation. Together, obtained results bring important insights regarding adverse effects and possible interaction of the three agrochemicals whose residues are commonly detected in agro-food products. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:46:14Z 2021-06-25T10:46:14Z 2021-01-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.tox.2020.152636 Toxicology, v. 447. 1879-3185 0300-483X http://hdl.handle.net/11449/206932 10.1016/j.tox.2020.152636 2-s2.0-85097217244 |
url |
http://dx.doi.org/10.1016/j.tox.2020.152636 http://hdl.handle.net/11449/206932 |
identifier_str_mv |
Toxicology, v. 447. 1879-3185 0300-483X 10.1016/j.tox.2020.152636 2-s2.0-85097217244 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965193655025664 |