Temperature effect on dextransucrase production by Leuconostoc mesenteroides FT 045 B isolated from Alcohol and Sugar Mill Plant

• Autor(a) principal: Cortezi, M.
• Data de Publicação: 2005
• Outros Autores: Monti, R., Contiero, Jonas [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: https://tspace.library.utoronto.ca/handle/1807/6629; http://hdl.handle.net/11449/20055
• topic: dextran; dextransucrase; Leuconostoc mesenteroides NRRL B512 (F); Leuconostoc mesenteroides FT045 B; temperature; enzyme activity

Temperature (23 to 31 degrees C) and sucrose concentration ( 3 and 4%) effects on dextransucrase production by Leuconostoc mesenteroides NRRL B 512 ( F) and Leuconostoc mesenteroides FT 045 B were studied. The conditions in all fermentations were: total reaction volume 2 L, 132 rev. min-1, 0.5 vvm and pH 6.0. The optimum temperature for enzyme yield for strain NRRL B 512 ( F) was 23 degrees C, where at 8-h fermentation was possible to achieve 49.3 DSU/mL. When FT 045 B strain was utilized, 3.2 DSU/mL was obtained at temperature 23 to 25 degrees C.