Production of monoclonal antibodies against Streptococcus mutans antigens
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1806-83242006000400003 http://hdl.handle.net/11449/69140 |
Resumo: | Several studies have been conducted in the last decades aiming to obtain an anti-canies vaccine, however some studies have demonstrated cross reactivity between Streptococcus mutans surface antigens and the human cardiac tissue. In this work, the reactivity of five anti-Streptococcus mutans monoclonal antibodies (MoAb) (24A, 56G, G8, E8 and F6) was tested against oral streptococci, cardiac antigens and skeletal and cardiac myosins, aiming to evaluate the specificity of these MoAb. The hybrid producers of immunoglobulins of the IgG 2b class were cloned by limit dilution and expanded in vivo. MoAb were tested by ELISA. The hybrid 24A reacted with S. mutans CCT 1910, S. salivarius CCT 0365 and S. pyogenes T23. No reactivity difference was observed among the tested species. Cross reactivity with heart and cardiac myosin was not confirmed and only reaction with myosin of skeletal muscle was observed (p = 0.0381). The hybrid 56G reacted with all the tested microorganisms and there was statistically significant difference between S. mutans and S. pyogenes T23 (p < 0.001). This hybrid also reacted with myosin of skeletal muscle (p = 0.0095). C8, E8 and F6 presented low reactivity against oral streptococci strains and no reactivity against cardiac antigens. The data of this study showed that the 24A and 56G anti-S. mutans MoAb presented reactivity with S. pyogenes and S. salivarius, reinforcing the occurrence of common antigens between these species. The tested MoAb presented low cross-reactivity with myosin of skeletal muscle, but anti-heart activity could not be confirmed. |
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Production of monoclonal antibodies against Streptococcus mutans antigensAntibodiesMonoclonalStreptococcus mutansbacterial antigenbacterium antibodyimmunoglobulin Gmonoclonal antibodyStreptococcus vaccineanimalantibody specificityBagg albino mousebiosynthesiscell culturedental cariesdisease modelenzyme linked immunosorbent assayfemalehumanimmunologymouseswineAnimalsAntibodies, BacterialAntibodies, MonoclonalAntibody SpecificityAntigens, BacterialCells, CulturedDental CariesDisease Models, AnimalEnzyme-Linked Immunosorbent AssayFemaleHumansImmunoglobulin GMiceMice, Inbred BALB CStreptococcal VaccinesSwineSeveral studies have been conducted in the last decades aiming to obtain an anti-canies vaccine, however some studies have demonstrated cross reactivity between Streptococcus mutans surface antigens and the human cardiac tissue. In this work, the reactivity of five anti-Streptococcus mutans monoclonal antibodies (MoAb) (24A, 56G, G8, E8 and F6) was tested against oral streptococci, cardiac antigens and skeletal and cardiac myosins, aiming to evaluate the specificity of these MoAb. The hybrid producers of immunoglobulins of the IgG 2b class were cloned by limit dilution and expanded in vivo. MoAb were tested by ELISA. The hybrid 24A reacted with S. mutans CCT 1910, S. salivarius CCT 0365 and S. pyogenes T23. No reactivity difference was observed among the tested species. Cross reactivity with heart and cardiac myosin was not confirmed and only reaction with myosin of skeletal muscle was observed (p = 0.0381). The hybrid 56G reacted with all the tested microorganisms and there was statistically significant difference between S. mutans and S. pyogenes T23 (p < 0.001). This hybrid also reacted with myosin of skeletal muscle (p = 0.0095). C8, E8 and F6 presented low reactivity against oral streptococci strains and no reactivity against cardiac antigens. The data of this study showed that the 24A and 56G anti-S. mutans MoAb presented reactivity with S. pyogenes and S. salivarius, reinforcing the occurrence of common antigens between these species. The tested MoAb presented low cross-reactivity with myosin of skeletal muscle, but anti-heart activity could not be confirmed.Department of Biosciences and Oral Diagnosis School of Dentistry of São José dos Campos São Paulo State University, São PauloDepartment of Microbiology and Immunology University of Mogi das Cruzes, Mogi das CruzesDepartment of Neurology University of São Paulo, São PauloDepartment of Biosciences and Oral Diagnosis School of Dentistry of São José dos Campos São Paulo State University, São PauloUniversidade Estadual Paulista (Unesp)University of Mogi das CruzesUniversidade de São Paulo (USP)Victor Canettieri, Antonio Carlos [UNESP]Kretchetoff, Fujiko YamasiroKoga Ito, Cristiane Yumi [UNESP]Moreira, Daniella [UNESP]Condino Fujarra, Fabio JoséUnterkircher, Carmelinda Schmidt [UNESP]2014-05-27T11:21:59Z2014-05-27T11:21:59Z2006-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article297-302application/pdfhttp://dx.doi.org/10.1590/S1806-83242006000400003Brazilian Oral Research, v. 20, n. 4, p. 297-302, 2006.1806-83241807-3107http://hdl.handle.net/11449/6914010.1590/S1806-83242006000400003S1806-832420060004000032-s2.0-338468467082-s2.0-33846846708.pdf6543563161403421Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Oral Research1.223info:eu-repo/semantics/openAccess2023-10-30T06:07:23Zoai:repositorio.unesp.br:11449/69140Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-30T06:07:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Production of monoclonal antibodies against Streptococcus mutans antigens |
title |
Production of monoclonal antibodies against Streptococcus mutans antigens |
spellingShingle |
Production of monoclonal antibodies against Streptococcus mutans antigens Victor Canettieri, Antonio Carlos [UNESP] Antibodies Monoclonal Streptococcus mutans bacterial antigen bacterium antibody immunoglobulin G monoclonal antibody Streptococcus vaccine animal antibody specificity Bagg albino mouse biosynthesis cell culture dental caries disease model enzyme linked immunosorbent assay female human immunology mouse swine Animals Antibodies, Bacterial Antibodies, Monoclonal Antibody Specificity Antigens, Bacterial Cells, Cultured Dental Caries Disease Models, Animal Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin G Mice Mice, Inbred BALB C Streptococcal Vaccines Swine |
title_short |
Production of monoclonal antibodies against Streptococcus mutans antigens |
title_full |
Production of monoclonal antibodies against Streptococcus mutans antigens |
title_fullStr |
Production of monoclonal antibodies against Streptococcus mutans antigens |
title_full_unstemmed |
Production of monoclonal antibodies against Streptococcus mutans antigens |
title_sort |
Production of monoclonal antibodies against Streptococcus mutans antigens |
author |
Victor Canettieri, Antonio Carlos [UNESP] |
author_facet |
Victor Canettieri, Antonio Carlos [UNESP] Kretchetoff, Fujiko Yamasiro Koga Ito, Cristiane Yumi [UNESP] Moreira, Daniella [UNESP] Condino Fujarra, Fabio José Unterkircher, Carmelinda Schmidt [UNESP] |
author_role |
author |
author2 |
Kretchetoff, Fujiko Yamasiro Koga Ito, Cristiane Yumi [UNESP] Moreira, Daniella [UNESP] Condino Fujarra, Fabio José Unterkircher, Carmelinda Schmidt [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Mogi das Cruzes Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Victor Canettieri, Antonio Carlos [UNESP] Kretchetoff, Fujiko Yamasiro Koga Ito, Cristiane Yumi [UNESP] Moreira, Daniella [UNESP] Condino Fujarra, Fabio José Unterkircher, Carmelinda Schmidt [UNESP] |
dc.subject.por.fl_str_mv |
Antibodies Monoclonal Streptococcus mutans bacterial antigen bacterium antibody immunoglobulin G monoclonal antibody Streptococcus vaccine animal antibody specificity Bagg albino mouse biosynthesis cell culture dental caries disease model enzyme linked immunosorbent assay female human immunology mouse swine Animals Antibodies, Bacterial Antibodies, Monoclonal Antibody Specificity Antigens, Bacterial Cells, Cultured Dental Caries Disease Models, Animal Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin G Mice Mice, Inbred BALB C Streptococcal Vaccines Swine |
topic |
Antibodies Monoclonal Streptococcus mutans bacterial antigen bacterium antibody immunoglobulin G monoclonal antibody Streptococcus vaccine animal antibody specificity Bagg albino mouse biosynthesis cell culture dental caries disease model enzyme linked immunosorbent assay female human immunology mouse swine Animals Antibodies, Bacterial Antibodies, Monoclonal Antibody Specificity Antigens, Bacterial Cells, Cultured Dental Caries Disease Models, Animal Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin G Mice Mice, Inbred BALB C Streptococcal Vaccines Swine |
description |
Several studies have been conducted in the last decades aiming to obtain an anti-canies vaccine, however some studies have demonstrated cross reactivity between Streptococcus mutans surface antigens and the human cardiac tissue. In this work, the reactivity of five anti-Streptococcus mutans monoclonal antibodies (MoAb) (24A, 56G, G8, E8 and F6) was tested against oral streptococci, cardiac antigens and skeletal and cardiac myosins, aiming to evaluate the specificity of these MoAb. The hybrid producers of immunoglobulins of the IgG 2b class were cloned by limit dilution and expanded in vivo. MoAb were tested by ELISA. The hybrid 24A reacted with S. mutans CCT 1910, S. salivarius CCT 0365 and S. pyogenes T23. No reactivity difference was observed among the tested species. Cross reactivity with heart and cardiac myosin was not confirmed and only reaction with myosin of skeletal muscle was observed (p = 0.0381). The hybrid 56G reacted with all the tested microorganisms and there was statistically significant difference between S. mutans and S. pyogenes T23 (p < 0.001). This hybrid also reacted with myosin of skeletal muscle (p = 0.0095). C8, E8 and F6 presented low reactivity against oral streptococci strains and no reactivity against cardiac antigens. The data of this study showed that the 24A and 56G anti-S. mutans MoAb presented reactivity with S. pyogenes and S. salivarius, reinforcing the occurrence of common antigens between these species. The tested MoAb presented low cross-reactivity with myosin of skeletal muscle, but anti-heart activity could not be confirmed. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-10-01 2014-05-27T11:21:59Z 2014-05-27T11:21:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1806-83242006000400003 Brazilian Oral Research, v. 20, n. 4, p. 297-302, 2006. 1806-8324 1807-3107 http://hdl.handle.net/11449/69140 10.1590/S1806-83242006000400003 S1806-83242006000400003 2-s2.0-33846846708 2-s2.0-33846846708.pdf 6543563161403421 |
url |
http://dx.doi.org/10.1590/S1806-83242006000400003 http://hdl.handle.net/11449/69140 |
identifier_str_mv |
Brazilian Oral Research, v. 20, n. 4, p. 297-302, 2006. 1806-8324 1807-3107 10.1590/S1806-83242006000400003 S1806-83242006000400003 2-s2.0-33846846708 2-s2.0-33846846708.pdf 6543563161403421 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Oral Research 1.223 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
297-302 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964754507202560 |