Proteômica como estratégia para estudos dos mecanismos de ativação de mastócitos de ratos causados pelo peptídeo Protopolybia MP-III

• Autor(a) principal: Garcia, Ana Maria Caviquioli [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/131735; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2015-10-15/000852543.pdf
• topic: Vespa; Proteômica; Vespa - Veneno; Mastócitos; Espectrometria de massa; Proteínas; Peptídeos

During the last decades, the Secretariat of Health Surveillance reports the growing number of accidents caused by bees and wasps. The venoms of social Hymenoptera diversity present in peptides and proteins, which act in allergic processes and frameworks, are associated with poisoning and vasoactive responses. In venoms of social wasps, one of the biologically active components greater importance among the peptides are degranulate mast cells, known as mastoparanos. The present work aimed to compose in the action of mastoparan Protopolybia MP-III, found the wasp neotropial Polybia exigua exigua in relation to the mechanism of degranulation of mast cells, demonstrating the release of chemical mediators, and the identification of allergenic proteins involved with the process by proteomic analysis and mass spectrometry. The proteomic analysis is characterized as a sensitive analytical technique with high standard of separation, when combined with mass spectrometry, allows the characterization of proteins with high accuracy. Then, the samples were analyzed by mass spectrometry with the use of a system LCMS-IT-TOF and identified by summing the total of 622 proteins which had metabolism-related cell adhesion, apoptosis, self protection, endocytic, exocytic functions, house keeping, energy metabolism, proteasome, mast cell function, cytoskeletal reorganization and signal transduction. The development of this project was conducted through a shotgun proteomics approach, which corroborated to a better understanding of the mechanisms of action of mastoparan Protopolybia MP-III as well as the understanding of inflammatory processes caused by the toxin after frequent accidents