Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathways
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jep.2017.03.059 http://hdl.handle.net/11449/174555 |
Resumo: | Ethnopharmacological relevance Casearia sylvestris Sw. is widely used in popular medicine to treat conditions associated with pain. Aim of the study The present study investigated the influence of hydroalcoholic crude extract of Casearia sylvestris (HCE-CS) and contribution of pro-resolving mediators on mechanical hyperalgesia in a mouse model of chronic post-ischemia pain (CPIP). Methods and results Male Swiss mice were subjected to ischemia of the right hind paw (3 h), then reperfusion was allowed. At 10 min, 24 h or 48 h post-ischemia/reperfusion (I/R), different groups of animals were treated with HCE-CS (30 mg/Kg, orally [p.o]), selected agonists at the pro-resolving receptor ALX/FPR2 (natural molecules like resolvin D1 and lipoxin A4 or the synthetic compound BML-111; 0.1–1 µg/animal) or vehicle (saline, 10 mL/Kg, s.c.), in the absence or presence of the antagonist WRW4 (10 µg, s.c.). Mechanical hyperalgesia (paw withdrawal to von Frey filament) was asseseed together with histological and immunostainning analyses. In these settings, pro-resolving mediators reduced mechanical hyperalgesia and HCE-CS or BML-111 displayed anti-hyperalgesic effects which was markedly attenuated in animals treated with WRW4. ALX/FPR2 expression was raised in skeletal muscle or neutrophils after treatment with HCE-CS or BML-111. Conclusion These results reveal significant antihyperalgesic effect of HCE-CS on CPIP, mediated at least in part, by the pathway of resolution of inflammation centred on the axis modulated by ALX/FPR2. |
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Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathwaysALX/FPR2Casearia sylvestrisChronic post-ischemia painInflammationSalicaceaeEthnopharmacological relevance Casearia sylvestris Sw. is widely used in popular medicine to treat conditions associated with pain. Aim of the study The present study investigated the influence of hydroalcoholic crude extract of Casearia sylvestris (HCE-CS) and contribution of pro-resolving mediators on mechanical hyperalgesia in a mouse model of chronic post-ischemia pain (CPIP). Methods and results Male Swiss mice were subjected to ischemia of the right hind paw (3 h), then reperfusion was allowed. At 10 min, 24 h or 48 h post-ischemia/reperfusion (I/R), different groups of animals were treated with HCE-CS (30 mg/Kg, orally [p.o]), selected agonists at the pro-resolving receptor ALX/FPR2 (natural molecules like resolvin D1 and lipoxin A4 or the synthetic compound BML-111; 0.1–1 µg/animal) or vehicle (saline, 10 mL/Kg, s.c.), in the absence or presence of the antagonist WRW4 (10 µg, s.c.). Mechanical hyperalgesia (paw withdrawal to von Frey filament) was asseseed together with histological and immunostainning analyses. In these settings, pro-resolving mediators reduced mechanical hyperalgesia and HCE-CS or BML-111 displayed anti-hyperalgesic effects which was markedly attenuated in animals treated with WRW4. ALX/FPR2 expression was raised in skeletal muscle or neutrophils after treatment with HCE-CS or BML-111. Conclusion These results reveal significant antihyperalgesic effect of HCE-CS on CPIP, mediated at least in part, by the pathway of resolution of inflammation centred on the axis modulated by ALX/FPR2.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Post-Graduate Programm in Health Science – Southern Univeristy of Santa Catarina (UNISUL)Laboratory of Experimental Neuroscience (LANEX)– UNISULWilliam Harvey Research Institute – Queen Mary University of London/LondonUndergraduation in Medicine – UNISULLaboratory of Neurobiology of Pain and Inflammation – UFSCDepartment of Morphological Science – UFSCDepartment of Organic Chemistry/Institute of Chemistry – UNESPDepartment of Organic Chemistry/Institute of Chemistry – UNESPCNPq: 608765Post-Graduate Programm in Health Science – Southern Univeristy of Santa Catarina (UNISUL)Laboratory of Experimental Neuroscience (LANEX)– UNISULWilliam Harvey Research Institute – Queen Mary University of London/LondonUndergraduation in Medicine – UNISULUniversidade Federal de Santa Catarina (UFSC)Universidade Estadual Paulista (Unesp)Piovezan, Anna P.Batisti, Ana P.Benevides, Maria L.A.C.S.Turnes, Bruna L.Martins, Daniel F.Kanis, LuizDuarte, Elisa C.W.Cavalheiro, Alberto J. [UNESP]Bueno, Paula C.P. [UNESP]Seed, Michael P.Norling, Lucy V.Cooper, DianneHeadland, SarahSouza, Patrícia R.P.S.Perretti, Mauro2018-12-11T17:11:42Z2018-12-11T17:11:42Z2017-05-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article179-188application/pdfhttp://dx.doi.org/10.1016/j.jep.2017.03.059Journal of Ethnopharmacology, v. 204, p. 179-188.1872-75730378-8741http://hdl.handle.net/11449/17455510.1016/j.jep.2017.03.0592-s2.0-850190488282-s2.0-85019048828.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Ethnopharmacology1,150info:eu-repo/semantics/openAccess2023-12-03T06:17:02Zoai:repositorio.unesp.br:11449/174555Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:24:57.762296Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathways |
title |
Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathways |
spellingShingle |
Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathways Piovezan, Anna P. ALX/FPR2 Casearia sylvestris Chronic post-ischemia pain Inflammation Salicaceae |
title_short |
Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathways |
title_full |
Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathways |
title_fullStr |
Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathways |
title_full_unstemmed |
Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathways |
title_sort |
Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathways |
author |
Piovezan, Anna P. |
author_facet |
Piovezan, Anna P. Batisti, Ana P. Benevides, Maria L.A.C.S. Turnes, Bruna L. Martins, Daniel F. Kanis, Luiz Duarte, Elisa C.W. Cavalheiro, Alberto J. [UNESP] Bueno, Paula C.P. [UNESP] Seed, Michael P. Norling, Lucy V. Cooper, Dianne Headland, Sarah Souza, Patrícia R.P.S. Perretti, Mauro |
author_role |
author |
author2 |
Batisti, Ana P. Benevides, Maria L.A.C.S. Turnes, Bruna L. Martins, Daniel F. Kanis, Luiz Duarte, Elisa C.W. Cavalheiro, Alberto J. [UNESP] Bueno, Paula C.P. [UNESP] Seed, Michael P. Norling, Lucy V. Cooper, Dianne Headland, Sarah Souza, Patrícia R.P.S. Perretti, Mauro |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Post-Graduate Programm in Health Science – Southern Univeristy of Santa Catarina (UNISUL) Laboratory of Experimental Neuroscience (LANEX)– UNISUL William Harvey Research Institute – Queen Mary University of London/London Undergraduation in Medicine – UNISUL Universidade Federal de Santa Catarina (UFSC) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Piovezan, Anna P. Batisti, Ana P. Benevides, Maria L.A.C.S. Turnes, Bruna L. Martins, Daniel F. Kanis, Luiz Duarte, Elisa C.W. Cavalheiro, Alberto J. [UNESP] Bueno, Paula C.P. [UNESP] Seed, Michael P. Norling, Lucy V. Cooper, Dianne Headland, Sarah Souza, Patrícia R.P.S. Perretti, Mauro |
dc.subject.por.fl_str_mv |
ALX/FPR2 Casearia sylvestris Chronic post-ischemia pain Inflammation Salicaceae |
topic |
ALX/FPR2 Casearia sylvestris Chronic post-ischemia pain Inflammation Salicaceae |
description |
Ethnopharmacological relevance Casearia sylvestris Sw. is widely used in popular medicine to treat conditions associated with pain. Aim of the study The present study investigated the influence of hydroalcoholic crude extract of Casearia sylvestris (HCE-CS) and contribution of pro-resolving mediators on mechanical hyperalgesia in a mouse model of chronic post-ischemia pain (CPIP). Methods and results Male Swiss mice were subjected to ischemia of the right hind paw (3 h), then reperfusion was allowed. At 10 min, 24 h or 48 h post-ischemia/reperfusion (I/R), different groups of animals were treated with HCE-CS (30 mg/Kg, orally [p.o]), selected agonists at the pro-resolving receptor ALX/FPR2 (natural molecules like resolvin D1 and lipoxin A4 or the synthetic compound BML-111; 0.1–1 µg/animal) or vehicle (saline, 10 mL/Kg, s.c.), in the absence or presence of the antagonist WRW4 (10 µg, s.c.). Mechanical hyperalgesia (paw withdrawal to von Frey filament) was asseseed together with histological and immunostainning analyses. In these settings, pro-resolving mediators reduced mechanical hyperalgesia and HCE-CS or BML-111 displayed anti-hyperalgesic effects which was markedly attenuated in animals treated with WRW4. ALX/FPR2 expression was raised in skeletal muscle or neutrophils after treatment with HCE-CS or BML-111. Conclusion These results reveal significant antihyperalgesic effect of HCE-CS on CPIP, mediated at least in part, by the pathway of resolution of inflammation centred on the axis modulated by ALX/FPR2. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-23 2018-12-11T17:11:42Z 2018-12-11T17:11:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jep.2017.03.059 Journal of Ethnopharmacology, v. 204, p. 179-188. 1872-7573 0378-8741 http://hdl.handle.net/11449/174555 10.1016/j.jep.2017.03.059 2-s2.0-85019048828 2-s2.0-85019048828.pdf |
url |
http://dx.doi.org/10.1016/j.jep.2017.03.059 http://hdl.handle.net/11449/174555 |
identifier_str_mv |
Journal of Ethnopharmacology, v. 204, p. 179-188. 1872-7573 0378-8741 10.1016/j.jep.2017.03.059 2-s2.0-85019048828 2-s2.0-85019048828.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Ethnopharmacology 1,150 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
179-188 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129064935882752 |