Cytotoxicity and degree of conversion of methacrylate and silorane

Detalhes bibliográficos
Autor(a) principal: Silva, Virgínia Angélica
Data de Publicação: 2020
Outros Autores: Gontijo, Sávio Morato de Lacerda, Gatti, Alexandre [UNESP], Poletto, Luiz Thadeu de Abreu, Alvim, Hugo Henriques
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.20396/bjos.v19i0.8660656
http://hdl.handle.net/11449/205653
Resumo: Composites have been proven to have a cytotoxic effect on a variety of tissues and cells. Aim: The aim of this study was to analyse the degree of conversion of resins and its correlation with the cell viability in primary gingival fibroblasts. Methods: Resin-based silorane (Filtek P90) and conventional methacrylate resins (Filtek Z100, Filtek Z250 and Filtek Z350XT) were used to evaluate cell viability and the degree of conversion. The resins were light-cured by a LED for 20 and 40 seconds. The degree of conversion was analysed by Fourier transform infrared spectroscopy. Cellular metabolism was evaluated after 24 hours by the MTT assay (n = 6) using the storage solution of composite resin for either 24 hours or 12 days. Variance analysis (ANOVA) with a Bonferroni correction (p < 0.05) was performed to compare the groups. Results: The composite Filtek P90 showed a higher degree of conversion when polymerised for 40 or 20 seconds, while the composites Filtek Z100, Filtek Z250 and Filtek Z350XT showed similar degree of conversion. Only the Filtek Z100 resin was cytotoxic. Conclusion: We found no statistically significant correlation between cell viability and the degree of conversion.
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spelling Cytotoxicity and degree of conversion of methacrylate and siloraneComposite resinsCytotoxinsFibroblastsComposites have been proven to have a cytotoxic effect on a variety of tissues and cells. Aim: The aim of this study was to analyse the degree of conversion of resins and its correlation with the cell viability in primary gingival fibroblasts. Methods: Resin-based silorane (Filtek P90) and conventional methacrylate resins (Filtek Z100, Filtek Z250 and Filtek Z350XT) were used to evaluate cell viability and the degree of conversion. The resins were light-cured by a LED for 20 and 40 seconds. The degree of conversion was analysed by Fourier transform infrared spectroscopy. Cellular metabolism was evaluated after 24 hours by the MTT assay (n = 6) using the storage solution of composite resin for either 24 hours or 12 days. Variance analysis (ANOVA) with a Bonferroni correction (p < 0.05) was performed to compare the groups. Results: The composite Filtek P90 showed a higher degree of conversion when polymerised for 40 or 20 seconds, while the composites Filtek Z100, Filtek Z250 and Filtek Z350XT showed similar degree of conversion. Only the Filtek Z100 resin was cytotoxic. Conclusion: We found no statistically significant correlation between cell viability and the degree of conversion.Department of Restorative Dentistry Faculty of Dentistry Federal University of Minas Gerais (UFMG)Department of Chemistry Paulista State University (UNESP)Department of Chemistry Paulista State University (UNESP)Universidade Federal de Minas Gerais (UFMG)Universidade Estadual Paulista (Unesp)Silva, Virgínia AngélicaGontijo, Sávio Morato de LacerdaGatti, Alexandre [UNESP]Poletto, Luiz Thadeu de AbreuAlvim, Hugo Henriques2021-06-25T10:19:01Z2021-06-25T10:19:01Z2020-01-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-9http://dx.doi.org/10.20396/bjos.v19i0.8660656Brazilian Journal of Oral Sciences, v. 19, p. 1-9.1677-32251677-3217http://hdl.handle.net/11449/20565310.20396/bjos.v19i0.86606562-s2.0-85098513605Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Oral Sciencesinfo:eu-repo/semantics/openAccess2021-10-22T12:58:34Zoai:repositorio.unesp.br:11449/205653Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:01:43.405933Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cytotoxicity and degree of conversion of methacrylate and silorane
title Cytotoxicity and degree of conversion of methacrylate and silorane
spellingShingle Cytotoxicity and degree of conversion of methacrylate and silorane
Silva, Virgínia Angélica
Composite resins
Cytotoxins
Fibroblasts
title_short Cytotoxicity and degree of conversion of methacrylate and silorane
title_full Cytotoxicity and degree of conversion of methacrylate and silorane
title_fullStr Cytotoxicity and degree of conversion of methacrylate and silorane
title_full_unstemmed Cytotoxicity and degree of conversion of methacrylate and silorane
title_sort Cytotoxicity and degree of conversion of methacrylate and silorane
author Silva, Virgínia Angélica
author_facet Silva, Virgínia Angélica
Gontijo, Sávio Morato de Lacerda
Gatti, Alexandre [UNESP]
Poletto, Luiz Thadeu de Abreu
Alvim, Hugo Henriques
author_role author
author2 Gontijo, Sávio Morato de Lacerda
Gatti, Alexandre [UNESP]
Poletto, Luiz Thadeu de Abreu
Alvim, Hugo Henriques
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Minas Gerais (UFMG)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva, Virgínia Angélica
Gontijo, Sávio Morato de Lacerda
Gatti, Alexandre [UNESP]
Poletto, Luiz Thadeu de Abreu
Alvim, Hugo Henriques
dc.subject.por.fl_str_mv Composite resins
Cytotoxins
Fibroblasts
topic Composite resins
Cytotoxins
Fibroblasts
description Composites have been proven to have a cytotoxic effect on a variety of tissues and cells. Aim: The aim of this study was to analyse the degree of conversion of resins and its correlation with the cell viability in primary gingival fibroblasts. Methods: Resin-based silorane (Filtek P90) and conventional methacrylate resins (Filtek Z100, Filtek Z250 and Filtek Z350XT) were used to evaluate cell viability and the degree of conversion. The resins were light-cured by a LED for 20 and 40 seconds. The degree of conversion was analysed by Fourier transform infrared spectroscopy. Cellular metabolism was evaluated after 24 hours by the MTT assay (n = 6) using the storage solution of composite resin for either 24 hours or 12 days. Variance analysis (ANOVA) with a Bonferroni correction (p < 0.05) was performed to compare the groups. Results: The composite Filtek P90 showed a higher degree of conversion when polymerised for 40 or 20 seconds, while the composites Filtek Z100, Filtek Z250 and Filtek Z350XT showed similar degree of conversion. Only the Filtek Z100 resin was cytotoxic. Conclusion: We found no statistically significant correlation between cell viability and the degree of conversion.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-27
2021-06-25T10:19:01Z
2021-06-25T10:19:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.20396/bjos.v19i0.8660656
Brazilian Journal of Oral Sciences, v. 19, p. 1-9.
1677-3225
1677-3217
http://hdl.handle.net/11449/205653
10.20396/bjos.v19i0.8660656
2-s2.0-85098513605
url http://dx.doi.org/10.20396/bjos.v19i0.8660656
http://hdl.handle.net/11449/205653
identifier_str_mv Brazilian Journal of Oral Sciences, v. 19, p. 1-9.
1677-3225
1677-3217
10.20396/bjos.v19i0.8660656
2-s2.0-85098513605
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Oral Sciences
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-9
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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