Microbiological assay for the determination of colistin sulphate

Detalhes bibliográficos
Autor(a) principal: Calixto, Giovana [UNESP]
Data de Publicação: 2014
Outros Autores: Domingos, Luciana Elisete, Fabri, Nathalie Thomazini, do Carmo, Thais Adriana, Moreno, Andréia de Haro [UNESP], Salgado, Hérida Regina Nunes [UNESP], Chorilli, Marlus [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.2174/15734110113090990017
http://hdl.handle.net/11449/227892
Resumo: An innovative microbiological assay was developed for the determination of colistin sulphate at various concentrations (192-469 IU/mL); using Bordetella bronchiseptica ATCC 4617 as microorganism test. This assay revealed subtle changes not demonstrated by chemical methods, such as chemical degradation and low of potency, but by evaluating the potency of colistin, which is very important for the analysis of antibiotics. The method was validated according to ICH guidelines, proving to be linear (r2 = 0.9985), precise (CV = 1.32%) and accurate (100.9 ± 1.33%). It was concluded that the microbiological assay is satisfactory for in vitro quantitation of the antibacterial activity of colistin sulphate.
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spelling Microbiological assay for the determination of colistin sulphateAgar diffusion methodBordetella bronchisepticaColistin sulphateIn vitro quantificationMethod validationMicrobiological assayAn innovative microbiological assay was developed for the determination of colistin sulphate at various concentrations (192-469 IU/mL); using Bordetella bronchiseptica ATCC 4617 as microorganism test. This assay revealed subtle changes not demonstrated by chemical methods, such as chemical degradation and low of potency, but by evaluating the potency of colistin, which is very important for the analysis of antibiotics. The method was validated according to ICH guidelines, proving to be linear (r2 = 0.9985), precise (CV = 1.32%) and accurate (100.9 ± 1.33%). It was concluded that the microbiological assay is satisfactory for in vitro quantitation of the antibacterial activity of colistin sulphate.School of Pharmaceutical Sciences Departament of Drugs and Pharmaceuticals São Paulo State University, Rodovia Araraquara/JaúSchool of Health Sciences Methodist University of Piracicaba, Rodovia do AçúcarSchool of Pharmaceutical Sciences Departament of Drugs and Pharmaceuticals São Paulo State University, Rodovia Araraquara/JaúUniversidade Estadual Paulista (UNESP)Methodist University of PiracicabaCalixto, Giovana [UNESP]Domingos, Luciana EliseteFabri, Nathalie Thomazinido Carmo, Thais AdrianaMoreno, Andréia de Haro [UNESP]Salgado, Hérida Regina Nunes [UNESP]Chorilli, Marlus [UNESP]2022-04-29T07:24:27Z2022-04-29T07:24:27Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article443-448http://dx.doi.org/10.2174/15734110113090990017Current Analytical Chemistry, v. 10, n. 3, p. 443-448, 2014.1573-4110http://hdl.handle.net/11449/22789210.2174/157341101130909900172-s2.0-84911994683Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCurrent Analytical Chemistryinfo:eu-repo/semantics/openAccess2024-06-24T13:45:30Zoai:repositorio.unesp.br:11449/227892Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:02:18.837403Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Microbiological assay for the determination of colistin sulphate
title Microbiological assay for the determination of colistin sulphate
spellingShingle Microbiological assay for the determination of colistin sulphate
Calixto, Giovana [UNESP]
Agar diffusion method
Bordetella bronchiseptica
Colistin sulphate
In vitro quantification
Method validation
Microbiological assay
title_short Microbiological assay for the determination of colistin sulphate
title_full Microbiological assay for the determination of colistin sulphate
title_fullStr Microbiological assay for the determination of colistin sulphate
title_full_unstemmed Microbiological assay for the determination of colistin sulphate
title_sort Microbiological assay for the determination of colistin sulphate
author Calixto, Giovana [UNESP]
author_facet Calixto, Giovana [UNESP]
Domingos, Luciana Elisete
Fabri, Nathalie Thomazini
do Carmo, Thais Adriana
Moreno, Andréia de Haro [UNESP]
Salgado, Hérida Regina Nunes [UNESP]
Chorilli, Marlus [UNESP]
author_role author
author2 Domingos, Luciana Elisete
Fabri, Nathalie Thomazini
do Carmo, Thais Adriana
Moreno, Andréia de Haro [UNESP]
Salgado, Hérida Regina Nunes [UNESP]
Chorilli, Marlus [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Methodist University of Piracicaba
dc.contributor.author.fl_str_mv Calixto, Giovana [UNESP]
Domingos, Luciana Elisete
Fabri, Nathalie Thomazini
do Carmo, Thais Adriana
Moreno, Andréia de Haro [UNESP]
Salgado, Hérida Regina Nunes [UNESP]
Chorilli, Marlus [UNESP]
dc.subject.por.fl_str_mv Agar diffusion method
Bordetella bronchiseptica
Colistin sulphate
In vitro quantification
Method validation
Microbiological assay
topic Agar diffusion method
Bordetella bronchiseptica
Colistin sulphate
In vitro quantification
Method validation
Microbiological assay
description An innovative microbiological assay was developed for the determination of colistin sulphate at various concentrations (192-469 IU/mL); using Bordetella bronchiseptica ATCC 4617 as microorganism test. This assay revealed subtle changes not demonstrated by chemical methods, such as chemical degradation and low of potency, but by evaluating the potency of colistin, which is very important for the analysis of antibiotics. The method was validated according to ICH guidelines, proving to be linear (r2 = 0.9985), precise (CV = 1.32%) and accurate (100.9 ± 1.33%). It was concluded that the microbiological assay is satisfactory for in vitro quantitation of the antibacterial activity of colistin sulphate.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
2022-04-29T07:24:27Z
2022-04-29T07:24:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.2174/15734110113090990017
Current Analytical Chemistry, v. 10, n. 3, p. 443-448, 2014.
1573-4110
http://hdl.handle.net/11449/227892
10.2174/15734110113090990017
2-s2.0-84911994683
url http://dx.doi.org/10.2174/15734110113090990017
http://hdl.handle.net/11449/227892
identifier_str_mv Current Analytical Chemistry, v. 10, n. 3, p. 443-448, 2014.
1573-4110
10.2174/15734110113090990017
2-s2.0-84911994683
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Current Analytical Chemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 443-448
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128743754956800

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