Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-77572010000400005 http://hdl.handle.net/11449/15118 |
Resumo: | Although the search for the ideal bone substitute has been the focus of a large number of studies, autogenous bone is still the gold standard for the filling of defects caused by pathologies and traumas, and mainly, for alveolar ridge reconstruction, allowing the titanium implants installation. Objectives: The aim of this study was to evaluate the dynamics of autogenous bone graft incorporation process to surgically created defects in rat calvaria, using epifluorescence microscopy. Material and methods: Five adult male rats weighing 200-300 g were used. The animals received two 5-mm-diameter bone defects bilaterally in each parietal bone with a trephine bur under general anesthesia. Two groups of defects were formed: a control group (n=5), in which the defects were filled with blood clot, and a graft group (n=5), in which the defects were filled with autogenous bone block, removed from the contralateral defect. The fluorochromes calcein and alizarin were applied at the 7th and 30th postoperative days, respectively. The animals were killed at 35 days. Results: The mineralization process was more intense in the graft group (32.09%) and occurred mainly between 7 and 30 days, the period labeled by calcein (24.66%). Conclusions: The fluorochromes showed to be appropriate to label mineralization areas. The interfacial areas between fluorochrome labels are important sources of information about the bone regeneration dynamics. |
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Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in ratsFluorescent dyesBone transplantationBone regenerationAlthough the search for the ideal bone substitute has been the focus of a large number of studies, autogenous bone is still the gold standard for the filling of defects caused by pathologies and traumas, and mainly, for alveolar ridge reconstruction, allowing the titanium implants installation. Objectives: The aim of this study was to evaluate the dynamics of autogenous bone graft incorporation process to surgically created defects in rat calvaria, using epifluorescence microscopy. Material and methods: Five adult male rats weighing 200-300 g were used. The animals received two 5-mm-diameter bone defects bilaterally in each parietal bone with a trephine bur under general anesthesia. Two groups of defects were formed: a control group (n=5), in which the defects were filled with blood clot, and a graft group (n=5), in which the defects were filled with autogenous bone block, removed from the contralateral defect. The fluorochromes calcein and alizarin were applied at the 7th and 30th postoperative days, respectively. The animals were killed at 35 days. Results: The mineralization process was more intense in the graft group (32.09%) and occurred mainly between 7 and 30 days, the period labeled by calcein (24.66%). Conclusions: The fluorochromes showed to be appropriate to label mineralization areas. The interfacial areas between fluorochrome labels are important sources of information about the bone regeneration dynamics.São Paulo State Univ UNESP, Discipline Oral & Maxillofacial Surg, Dept Surg & Integrated Clin, Aracatuba Dent Sch, Aracatuba, SP, BrazilSão Paulo State Univ UNESP, Discipline Oral & Maxillofacial Surg, Dept Surg & Integrated Clin, Aracatuba Dent Sch, Aracatuba, SP, BrazilUniversidade de São Paulo (USP), Faculdade de Odontologia de BauruUniversidade Estadual Paulista (Unesp)Guskuma, Marcos Heidy [UNESP]Hochuli-Vieira, Eduardo [UNESP]Pereira, Flavia Priscila [UNESP]Rangel-Garcia Junior, Idelmo [UNESP]Okamoto, Roberta [UNESP]Okamoto, Tetuo [UNESP]Magro Filho, Osvaldo [UNESP]2013-09-30T18:29:37Z2014-05-20T13:43:21Z2013-09-30T18:29:37Z2014-05-20T13:43:21Z2010-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject346-353application/pdfhttp://dx.doi.org/10.1590/S1678-77572010000400005Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 18, n. 4, p. 346-353, 2010.1678-7757http://hdl.handle.net/11449/15118S1678-77572010000400005WOS:000281692300005S1678-77572010000400005-en.pdf156692821982805601699553639680975535418670745125Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Science1.7090,645info:eu-repo/semantics/openAccess2023-12-07T06:14:32Zoai:repositorio.unesp.br:11449/15118Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:39:49.845974Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats |
title |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats |
spellingShingle |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats Guskuma, Marcos Heidy [UNESP] Fluorescent dyes Bone transplantation Bone regeneration |
title_short |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats |
title_full |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats |
title_fullStr |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats |
title_full_unstemmed |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats |
title_sort |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats |
author |
Guskuma, Marcos Heidy [UNESP] |
author_facet |
Guskuma, Marcos Heidy [UNESP] Hochuli-Vieira, Eduardo [UNESP] Pereira, Flavia Priscila [UNESP] Rangel-Garcia Junior, Idelmo [UNESP] Okamoto, Roberta [UNESP] Okamoto, Tetuo [UNESP] Magro Filho, Osvaldo [UNESP] |
author_role |
author |
author2 |
Hochuli-Vieira, Eduardo [UNESP] Pereira, Flavia Priscila [UNESP] Rangel-Garcia Junior, Idelmo [UNESP] Okamoto, Roberta [UNESP] Okamoto, Tetuo [UNESP] Magro Filho, Osvaldo [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Guskuma, Marcos Heidy [UNESP] Hochuli-Vieira, Eduardo [UNESP] Pereira, Flavia Priscila [UNESP] Rangel-Garcia Junior, Idelmo [UNESP] Okamoto, Roberta [UNESP] Okamoto, Tetuo [UNESP] Magro Filho, Osvaldo [UNESP] |
dc.subject.por.fl_str_mv |
Fluorescent dyes Bone transplantation Bone regeneration |
topic |
Fluorescent dyes Bone transplantation Bone regeneration |
description |
Although the search for the ideal bone substitute has been the focus of a large number of studies, autogenous bone is still the gold standard for the filling of defects caused by pathologies and traumas, and mainly, for alveolar ridge reconstruction, allowing the titanium implants installation. Objectives: The aim of this study was to evaluate the dynamics of autogenous bone graft incorporation process to surgically created defects in rat calvaria, using epifluorescence microscopy. Material and methods: Five adult male rats weighing 200-300 g were used. The animals received two 5-mm-diameter bone defects bilaterally in each parietal bone with a trephine bur under general anesthesia. Two groups of defects were formed: a control group (n=5), in which the defects were filled with blood clot, and a graft group (n=5), in which the defects were filled with autogenous bone block, removed from the contralateral defect. The fluorochromes calcein and alizarin were applied at the 7th and 30th postoperative days, respectively. The animals were killed at 35 days. Results: The mineralization process was more intense in the graft group (32.09%) and occurred mainly between 7 and 30 days, the period labeled by calcein (24.66%). Conclusions: The fluorochromes showed to be appropriate to label mineralization areas. The interfacial areas between fluorochrome labels are important sources of information about the bone regeneration dynamics. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-07-01 2013-09-30T18:29:37Z 2013-09-30T18:29:37Z 2014-05-20T13:43:21Z 2014-05-20T13:43:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-77572010000400005 Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 18, n. 4, p. 346-353, 2010. 1678-7757 http://hdl.handle.net/11449/15118 S1678-77572010000400005 WOS:000281692300005 S1678-77572010000400005-en.pdf 1566928219828056 0169955363968097 5535418670745125 |
url |
http://dx.doi.org/10.1590/S1678-77572010000400005 http://hdl.handle.net/11449/15118 |
identifier_str_mv |
Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 18, n. 4, p. 346-353, 2010. 1678-7757 S1678-77572010000400005 WOS:000281692300005 S1678-77572010000400005-en.pdf 1566928219828056 0169955363968097 5535418670745125 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Oral Science 1.709 0,645 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
346-353 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo (USP), Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo (USP), Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129103885238272 |