Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats

Detalhes bibliográficos
Autor(a) principal: Guskuma, Marcos Heidy
Data de Publicação: 2010
Outros Autores: Hochuli-Vieira, Eduardo, Pereira, Flávia Priscila, Rangel-Garcia Junior, Idelmo, Okamoto, Roberta, Okamoto, Tetuo, Magro Filho, Osvaldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/3807
Resumo: Although the search for the ideal bone substitute has been the focus of a large number of studies, autogenous bone is still the gold standard for the filling of defects caused by pathologies and traumas, and mainly, for alveolar ridge reconstruction, allowing the titanium implants installation. OBJECTIVES: The aim of this study was to evaluate the dynamics of autogenous bone graft incorporation process to surgically created defects in rat calvaria, using epifluorescence microscopy. MATERIAL AND METHODS: Five adult male rats weighing 200-300 g were used. The animals received two 5-mm-diameter bone defects bilaterally in each parietal bone with a trephine bur under general anesthesia. Two groups of defects were formed: a control group (n=5), in which the defects were filled with blood clot, and a graft group (n=5), in which the defects were filled with autogenous bone block, removed from the contralateral defect. The fluorochromes calcein and alizarin were applied at the 7th and 30th postoperative days, respectively. The animals were killed at 35 days. RESULTS: The mineralization process was more intense in the graft group (32.09%) and occurred mainly between 7 and 30 days, the period labeled by calcein (24.66%). CONCLUSIONS: The fluorochromes showed to be appropriate to label mineralization areas. The interfacial areas between fluorochrome labels are important sources of information about the bone regeneration dynamics.
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spelling Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats Fluorescent dyesBone transplantationBone regeneration Although the search for the ideal bone substitute has been the focus of a large number of studies, autogenous bone is still the gold standard for the filling of defects caused by pathologies and traumas, and mainly, for alveolar ridge reconstruction, allowing the titanium implants installation. OBJECTIVES: The aim of this study was to evaluate the dynamics of autogenous bone graft incorporation process to surgically created defects in rat calvaria, using epifluorescence microscopy. MATERIAL AND METHODS: Five adult male rats weighing 200-300 g were used. The animals received two 5-mm-diameter bone defects bilaterally in each parietal bone with a trephine bur under general anesthesia. Two groups of defects were formed: a control group (n=5), in which the defects were filled with blood clot, and a graft group (n=5), in which the defects were filled with autogenous bone block, removed from the contralateral defect. The fluorochromes calcein and alizarin were applied at the 7th and 30th postoperative days, respectively. The animals were killed at 35 days. RESULTS: The mineralization process was more intense in the graft group (32.09%) and occurred mainly between 7 and 30 days, the period labeled by calcein (24.66%). CONCLUSIONS: The fluorochromes showed to be appropriate to label mineralization areas. The interfacial areas between fluorochrome labels are important sources of information about the bone regeneration dynamics. Universidade de São Paulo. Faculdade de Odontologia de Bauru2010-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/380710.1590/S1678-77572010000400005Journal of Applied Oral Science; Vol. 18 No. 4 (2010); 346-353 Journal of Applied Oral Science; Vol. 18 Núm. 4 (2010); 346-353 Journal of Applied Oral Science; v. 18 n. 4 (2010); 346-353 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3807/4497Copyright (c) 2010 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessGuskuma, Marcos HeidyHochuli-Vieira, EduardoPereira, Flávia PriscilaRangel-Garcia Junior, IdelmoOkamoto, RobertaOkamoto, TetuoMagro Filho, Osvaldo2012-04-27T12:09:56Zoai:revistas.usp.br:article/3807Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T12:09:56Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats
title Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats
spellingShingle Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats
Guskuma, Marcos Heidy
Fluorescent dyes
Bone transplantation
Bone regeneration
title_short Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats
title_full Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats
title_fullStr Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats
title_full_unstemmed Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats
title_sort Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats
author Guskuma, Marcos Heidy
author_facet Guskuma, Marcos Heidy
Hochuli-Vieira, Eduardo
Pereira, Flávia Priscila
Rangel-Garcia Junior, Idelmo
Okamoto, Roberta
Okamoto, Tetuo
Magro Filho, Osvaldo
author_role author
author2 Hochuli-Vieira, Eduardo
Pereira, Flávia Priscila
Rangel-Garcia Junior, Idelmo
Okamoto, Roberta
Okamoto, Tetuo
Magro Filho, Osvaldo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Guskuma, Marcos Heidy
Hochuli-Vieira, Eduardo
Pereira, Flávia Priscila
Rangel-Garcia Junior, Idelmo
Okamoto, Roberta
Okamoto, Tetuo
Magro Filho, Osvaldo
dc.subject.por.fl_str_mv Fluorescent dyes
Bone transplantation
Bone regeneration
topic Fluorescent dyes
Bone transplantation
Bone regeneration
description Although the search for the ideal bone substitute has been the focus of a large number of studies, autogenous bone is still the gold standard for the filling of defects caused by pathologies and traumas, and mainly, for alveolar ridge reconstruction, allowing the titanium implants installation. OBJECTIVES: The aim of this study was to evaluate the dynamics of autogenous bone graft incorporation process to surgically created defects in rat calvaria, using epifluorescence microscopy. MATERIAL AND METHODS: Five adult male rats weighing 200-300 g were used. The animals received two 5-mm-diameter bone defects bilaterally in each parietal bone with a trephine bur under general anesthesia. Two groups of defects were formed: a control group (n=5), in which the defects were filled with blood clot, and a graft group (n=5), in which the defects were filled with autogenous bone block, removed from the contralateral defect. The fluorochromes calcein and alizarin were applied at the 7th and 30th postoperative days, respectively. The animals were killed at 35 days. RESULTS: The mineralization process was more intense in the graft group (32.09%) and occurred mainly between 7 and 30 days, the period labeled by calcein (24.66%). CONCLUSIONS: The fluorochromes showed to be appropriate to label mineralization areas. The interfacial areas between fluorochrome labels are important sources of information about the bone regeneration dynamics.
publishDate 2010
dc.date.none.fl_str_mv 2010-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3807
10.1590/S1678-77572010000400005
url https://www.revistas.usp.br/jaos/article/view/3807
identifier_str_mv 10.1590/S1678-77572010000400005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3807/4497
dc.rights.driver.fl_str_mv Copyright (c) 2010 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2010 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 18 No. 4 (2010); 346-353
Journal of Applied Oral Science; Vol. 18 Núm. 4 (2010); 346-353
Journal of Applied Oral Science; v. 18 n. 4 (2010); 346-353
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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