Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/3807 |
Resumo: | Although the search for the ideal bone substitute has been the focus of a large number of studies, autogenous bone is still the gold standard for the filling of defects caused by pathologies and traumas, and mainly, for alveolar ridge reconstruction, allowing the titanium implants installation. OBJECTIVES: The aim of this study was to evaluate the dynamics of autogenous bone graft incorporation process to surgically created defects in rat calvaria, using epifluorescence microscopy. MATERIAL AND METHODS: Five adult male rats weighing 200-300 g were used. The animals received two 5-mm-diameter bone defects bilaterally in each parietal bone with a trephine bur under general anesthesia. Two groups of defects were formed: a control group (n=5), in which the defects were filled with blood clot, and a graft group (n=5), in which the defects were filled with autogenous bone block, removed from the contralateral defect. The fluorochromes calcein and alizarin were applied at the 7th and 30th postoperative days, respectively. The animals were killed at 35 days. RESULTS: The mineralization process was more intense in the graft group (32.09%) and occurred mainly between 7 and 30 days, the period labeled by calcein (24.66%). CONCLUSIONS: The fluorochromes showed to be appropriate to label mineralization areas. The interfacial areas between fluorochrome labels are important sources of information about the bone regeneration dynamics. |
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Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats Fluorescent dyesBone transplantationBone regeneration Although the search for the ideal bone substitute has been the focus of a large number of studies, autogenous bone is still the gold standard for the filling of defects caused by pathologies and traumas, and mainly, for alveolar ridge reconstruction, allowing the titanium implants installation. OBJECTIVES: The aim of this study was to evaluate the dynamics of autogenous bone graft incorporation process to surgically created defects in rat calvaria, using epifluorescence microscopy. MATERIAL AND METHODS: Five adult male rats weighing 200-300 g were used. The animals received two 5-mm-diameter bone defects bilaterally in each parietal bone with a trephine bur under general anesthesia. Two groups of defects were formed: a control group (n=5), in which the defects were filled with blood clot, and a graft group (n=5), in which the defects were filled with autogenous bone block, removed from the contralateral defect. The fluorochromes calcein and alizarin were applied at the 7th and 30th postoperative days, respectively. The animals were killed at 35 days. RESULTS: The mineralization process was more intense in the graft group (32.09%) and occurred mainly between 7 and 30 days, the period labeled by calcein (24.66%). CONCLUSIONS: The fluorochromes showed to be appropriate to label mineralization areas. The interfacial areas between fluorochrome labels are important sources of information about the bone regeneration dynamics. Universidade de São Paulo. Faculdade de Odontologia de Bauru2010-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/380710.1590/S1678-77572010000400005Journal of Applied Oral Science; Vol. 18 No. 4 (2010); 346-353 Journal of Applied Oral Science; Vol. 18 Núm. 4 (2010); 346-353 Journal of Applied Oral Science; v. 18 n. 4 (2010); 346-353 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3807/4497Copyright (c) 2010 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessGuskuma, Marcos HeidyHochuli-Vieira, EduardoPereira, Flávia PriscilaRangel-Garcia Junior, IdelmoOkamoto, RobertaOkamoto, TetuoMagro Filho, Osvaldo2012-04-27T12:09:56Zoai:revistas.usp.br:article/3807Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T12:09:56Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats |
title |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats |
spellingShingle |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats Guskuma, Marcos Heidy Fluorescent dyes Bone transplantation Bone regeneration |
title_short |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats |
title_full |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats |
title_fullStr |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats |
title_full_unstemmed |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats |
title_sort |
Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats |
author |
Guskuma, Marcos Heidy |
author_facet |
Guskuma, Marcos Heidy Hochuli-Vieira, Eduardo Pereira, Flávia Priscila Rangel-Garcia Junior, Idelmo Okamoto, Roberta Okamoto, Tetuo Magro Filho, Osvaldo |
author_role |
author |
author2 |
Hochuli-Vieira, Eduardo Pereira, Flávia Priscila Rangel-Garcia Junior, Idelmo Okamoto, Roberta Okamoto, Tetuo Magro Filho, Osvaldo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Guskuma, Marcos Heidy Hochuli-Vieira, Eduardo Pereira, Flávia Priscila Rangel-Garcia Junior, Idelmo Okamoto, Roberta Okamoto, Tetuo Magro Filho, Osvaldo |
dc.subject.por.fl_str_mv |
Fluorescent dyes Bone transplantation Bone regeneration |
topic |
Fluorescent dyes Bone transplantation Bone regeneration |
description |
Although the search for the ideal bone substitute has been the focus of a large number of studies, autogenous bone is still the gold standard for the filling of defects caused by pathologies and traumas, and mainly, for alveolar ridge reconstruction, allowing the titanium implants installation. OBJECTIVES: The aim of this study was to evaluate the dynamics of autogenous bone graft incorporation process to surgically created defects in rat calvaria, using epifluorescence microscopy. MATERIAL AND METHODS: Five adult male rats weighing 200-300 g were used. The animals received two 5-mm-diameter bone defects bilaterally in each parietal bone with a trephine bur under general anesthesia. Two groups of defects were formed: a control group (n=5), in which the defects were filled with blood clot, and a graft group (n=5), in which the defects were filled with autogenous bone block, removed from the contralateral defect. The fluorochromes calcein and alizarin were applied at the 7th and 30th postoperative days, respectively. The animals were killed at 35 days. RESULTS: The mineralization process was more intense in the graft group (32.09%) and occurred mainly between 7 and 30 days, the period labeled by calcein (24.66%). CONCLUSIONS: The fluorochromes showed to be appropriate to label mineralization areas. The interfacial areas between fluorochrome labels are important sources of information about the bone regeneration dynamics. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3807 10.1590/S1678-77572010000400005 |
url |
https://www.revistas.usp.br/jaos/article/view/3807 |
identifier_str_mv |
10.1590/S1678-77572010000400005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3807/4497 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2010 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2010 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 18 No. 4 (2010); 346-353 Journal of Applied Oral Science; Vol. 18 Núm. 4 (2010); 346-353 Journal of Applied Oral Science; v. 18 n. 4 (2010); 346-353 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221675504009216 |