Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/209433 |
Resumo: | Severe muscle injuries are common in accidents and have a delayed recovery of muscle integrity. In these cases, muscle suture surgery is the standard treatment. However, Platelet Rich Plasma (PRP), has been widely used in orthopedic injuries due to its growth factors. Thus, the objective of the study will be to analyze the association of suture and PRP techniques in the collagen and tenacity of the injured muscle. Were used seventy rats, divided into five groups: control (C), injury control (CI), injury and suture (IS), injury and PRP (IP), injury, suture, and PRP (ISP). Were sectioned approximately 50 % of the width and 100 % of the thickness of the gastrocnemius muscle. The homologous PRP was applied 24h after the injury. On the 7th day after the injury, the animals were euthanized and their muscles subjected to mechanical testing to measure tenacity or collagen analysis to calculate the ratio between type I and III collagen. The results show a significant decrease (p <0.05) in the values of the relationship between collagens in all injured groups (CI, IS, IP, ISP) compared to group C. In injured groups, the tenacity was significantly (p <0.05) reduced compared to the control group, with no observed difference between treatments and injured groups. The amount of collagen in the injured area has increased, but it did not affect the tenacity of the muscles, which was reduced. |
id |
UNSP_c27084483ce6b76e31319c304107df3e |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/209433 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich PlasmaMuscle injuryPlatelet-rich plasmaSutureCollagenSevere muscle injuries are common in accidents and have a delayed recovery of muscle integrity. In these cases, muscle suture surgery is the standard treatment. However, Platelet Rich Plasma (PRP), has been widely used in orthopedic injuries due to its growth factors. Thus, the objective of the study will be to analyze the association of suture and PRP techniques in the collagen and tenacity of the injured muscle. Were used seventy rats, divided into five groups: control (C), injury control (CI), injury and suture (IS), injury and PRP (IP), injury, suture, and PRP (ISP). Were sectioned approximately 50 % of the width and 100 % of the thickness of the gastrocnemius muscle. The homologous PRP was applied 24h after the injury. On the 7th day after the injury, the animals were euthanized and their muscles subjected to mechanical testing to measure tenacity or collagen analysis to calculate the ratio between type I and III collagen. The results show a significant decrease (p <0.05) in the values of the relationship between collagens in all injured groups (CI, IS, IP, ISP) compared to group C. In injured groups, the tenacity was significantly (p <0.05) reduced compared to the control group, with no observed difference between treatments and injured groups. The amount of collagen in the injured area has increased, but it did not affect the tenacity of the muscles, which was reduced.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade do Oeste Paulista (Unoeste)Univ Estadual Campinas, Fac Ciencias Med, Ctr Med Expt & Cirurgia, Lab Biomat LABIMO, Sao Paulo, BrazilUniv Estadual Paulista UNIP, Dept Fisioterapia, Campus Aracatuba, Sao Paulo, BrazilUniv Oeste Paulista, Dept Educ Fis, Campus Presidente Prudente, Sao Paulo, BrazilUniv Estadual Paulista, Dept Fis, Campus Presidente Prudente, Sao Paulo, BrazilUniv Estadual Paulista, Dept Fisioterapia, Campus Presidente Prudente, Sao Paulo, BrazilUniv Estadual Campinas, Fac Ciencias, Dept Ortopedia & Traumatol, Sao Paulo, BrazilUniv Estadual Paulista UNIP, Dept Fisioterapia, Campus Aracatuba, Sao Paulo, BrazilUniv Estadual Paulista, Dept Fis, Campus Presidente Prudente, Sao Paulo, BrazilUniv Estadual Paulista, Dept Fisioterapia, Campus Presidente Prudente, Sao Paulo, BrazilFAPESP: 2017/06930-0Soc Chilena AnatomiaUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Univ Oeste PaulistaGarcia, Thiago AlvesTamura Ozaki, Guilherme Akio [UNESP]Castoldi, Robson ChaconJob, Aldo Eloizo [UNESP]Trindade Camargo, Regina Celi [UNESP]Silva Camargo Filho, Jose Carlos [UNESP]Belangero, William Dias2021-06-25T12:18:32Z2021-06-25T12:18:32Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1392-1397International Journal Of Morphology. Temuco: Soc Chilena Anatomia, v. 38, n. 5, p. 1392-1397, 2020.0717-9502http://hdl.handle.net/11449/209433WOS:000566485800036Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal Of Morphologyinfo:eu-repo/semantics/openAccess2024-06-18T18:44:29Zoai:repositorio.unesp.br:11449/209433Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:04:57.584360Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma |
title |
Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma |
spellingShingle |
Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma Garcia, Thiago Alves Muscle injury Platelet-rich plasma Suture Collagen |
title_short |
Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma |
title_full |
Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma |
title_fullStr |
Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma |
title_full_unstemmed |
Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma |
title_sort |
Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma |
author |
Garcia, Thiago Alves |
author_facet |
Garcia, Thiago Alves Tamura Ozaki, Guilherme Akio [UNESP] Castoldi, Robson Chacon Job, Aldo Eloizo [UNESP] Trindade Camargo, Regina Celi [UNESP] Silva Camargo Filho, Jose Carlos [UNESP] Belangero, William Dias |
author_role |
author |
author2 |
Tamura Ozaki, Guilherme Akio [UNESP] Castoldi, Robson Chacon Job, Aldo Eloizo [UNESP] Trindade Camargo, Regina Celi [UNESP] Silva Camargo Filho, Jose Carlos [UNESP] Belangero, William Dias |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) Univ Oeste Paulista |
dc.contributor.author.fl_str_mv |
Garcia, Thiago Alves Tamura Ozaki, Guilherme Akio [UNESP] Castoldi, Robson Chacon Job, Aldo Eloizo [UNESP] Trindade Camargo, Regina Celi [UNESP] Silva Camargo Filho, Jose Carlos [UNESP] Belangero, William Dias |
dc.subject.por.fl_str_mv |
Muscle injury Platelet-rich plasma Suture Collagen |
topic |
Muscle injury Platelet-rich plasma Suture Collagen |
description |
Severe muscle injuries are common in accidents and have a delayed recovery of muscle integrity. In these cases, muscle suture surgery is the standard treatment. However, Platelet Rich Plasma (PRP), has been widely used in orthopedic injuries due to its growth factors. Thus, the objective of the study will be to analyze the association of suture and PRP techniques in the collagen and tenacity of the injured muscle. Were used seventy rats, divided into five groups: control (C), injury control (CI), injury and suture (IS), injury and PRP (IP), injury, suture, and PRP (ISP). Were sectioned approximately 50 % of the width and 100 % of the thickness of the gastrocnemius muscle. The homologous PRP was applied 24h after the injury. On the 7th day after the injury, the animals were euthanized and their muscles subjected to mechanical testing to measure tenacity or collagen analysis to calculate the ratio between type I and III collagen. The results show a significant decrease (p <0.05) in the values of the relationship between collagens in all injured groups (CI, IS, IP, ISP) compared to group C. In injured groups, the tenacity was significantly (p <0.05) reduced compared to the control group, with no observed difference between treatments and injured groups. The amount of collagen in the injured area has increased, but it did not affect the tenacity of the muscles, which was reduced. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-01 2021-06-25T12:18:32Z 2021-06-25T12:18:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
International Journal Of Morphology. Temuco: Soc Chilena Anatomia, v. 38, n. 5, p. 1392-1397, 2020. 0717-9502 http://hdl.handle.net/11449/209433 WOS:000566485800036 |
identifier_str_mv |
International Journal Of Morphology. Temuco: Soc Chilena Anatomia, v. 38, n. 5, p. 1392-1397, 2020. 0717-9502 WOS:000566485800036 |
url |
http://hdl.handle.net/11449/209433 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal Of Morphology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1392-1397 |
dc.publisher.none.fl_str_mv |
Soc Chilena Anatomia |
publisher.none.fl_str_mv |
Soc Chilena Anatomia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129488047833088 |