Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma

Detalhes bibliográficos
Autor(a) principal: Garcia, Thiago Alves
Data de Publicação: 2020
Outros Autores: Tamura Ozaki, Guilherme Akio [UNESP], Castoldi, Robson Chacon, Job, Aldo Eloizo [UNESP], Trindade Camargo, Regina Celi [UNESP], Silva Camargo Filho, Jose Carlos [UNESP], Belangero, William Dias
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/209433
Resumo: Severe muscle injuries are common in accidents and have a delayed recovery of muscle integrity. In these cases, muscle suture surgery is the standard treatment. However, Platelet Rich Plasma (PRP), has been widely used in orthopedic injuries due to its growth factors. Thus, the objective of the study will be to analyze the association of suture and PRP techniques in the collagen and tenacity of the injured muscle. Were used seventy rats, divided into five groups: control (C), injury control (CI), injury and suture (IS), injury and PRP (IP), injury, suture, and PRP (ISP). Were sectioned approximately 50 % of the width and 100 % of the thickness of the gastrocnemius muscle. The homologous PRP was applied 24h after the injury. On the 7th day after the injury, the animals were euthanized and their muscles subjected to mechanical testing to measure tenacity or collagen analysis to calculate the ratio between type I and III collagen. The results show a significant decrease (p <0.05) in the values of the relationship between collagens in all injured groups (CI, IS, IP, ISP) compared to group C. In injured groups, the tenacity was significantly (p <0.05) reduced compared to the control group, with no observed difference between treatments and injured groups. The amount of collagen in the injured area has increased, but it did not affect the tenacity of the muscles, which was reduced.
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spelling Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich PlasmaMuscle injuryPlatelet-rich plasmaSutureCollagenSevere muscle injuries are common in accidents and have a delayed recovery of muscle integrity. In these cases, muscle suture surgery is the standard treatment. However, Platelet Rich Plasma (PRP), has been widely used in orthopedic injuries due to its growth factors. Thus, the objective of the study will be to analyze the association of suture and PRP techniques in the collagen and tenacity of the injured muscle. Were used seventy rats, divided into five groups: control (C), injury control (CI), injury and suture (IS), injury and PRP (IP), injury, suture, and PRP (ISP). Were sectioned approximately 50 % of the width and 100 % of the thickness of the gastrocnemius muscle. The homologous PRP was applied 24h after the injury. On the 7th day after the injury, the animals were euthanized and their muscles subjected to mechanical testing to measure tenacity or collagen analysis to calculate the ratio between type I and III collagen. The results show a significant decrease (p <0.05) in the values of the relationship between collagens in all injured groups (CI, IS, IP, ISP) compared to group C. In injured groups, the tenacity was significantly (p <0.05) reduced compared to the control group, with no observed difference between treatments and injured groups. The amount of collagen in the injured area has increased, but it did not affect the tenacity of the muscles, which was reduced.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade do Oeste Paulista (Unoeste)Univ Estadual Campinas, Fac Ciencias Med, Ctr Med Expt & Cirurgia, Lab Biomat LABIMO, Sao Paulo, BrazilUniv Estadual Paulista UNIP, Dept Fisioterapia, Campus Aracatuba, Sao Paulo, BrazilUniv Oeste Paulista, Dept Educ Fis, Campus Presidente Prudente, Sao Paulo, BrazilUniv Estadual Paulista, Dept Fis, Campus Presidente Prudente, Sao Paulo, BrazilUniv Estadual Paulista, Dept Fisioterapia, Campus Presidente Prudente, Sao Paulo, BrazilUniv Estadual Campinas, Fac Ciencias, Dept Ortopedia & Traumatol, Sao Paulo, BrazilUniv Estadual Paulista UNIP, Dept Fisioterapia, Campus Aracatuba, Sao Paulo, BrazilUniv Estadual Paulista, Dept Fis, Campus Presidente Prudente, Sao Paulo, BrazilUniv Estadual Paulista, Dept Fisioterapia, Campus Presidente Prudente, Sao Paulo, BrazilFAPESP: 2017/06930-0Soc Chilena AnatomiaUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Univ Oeste PaulistaGarcia, Thiago AlvesTamura Ozaki, Guilherme Akio [UNESP]Castoldi, Robson ChaconJob, Aldo Eloizo [UNESP]Trindade Camargo, Regina Celi [UNESP]Silva Camargo Filho, Jose Carlos [UNESP]Belangero, William Dias2021-06-25T12:18:32Z2021-06-25T12:18:32Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1392-1397International Journal Of Morphology. Temuco: Soc Chilena Anatomia, v. 38, n. 5, p. 1392-1397, 2020.0717-9502http://hdl.handle.net/11449/209433WOS:000566485800036Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal Of Morphologyinfo:eu-repo/semantics/openAccess2024-06-18T18:44:29Zoai:repositorio.unesp.br:11449/209433Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:04:57.584360Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma
title Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma
spellingShingle Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma
Garcia, Thiago Alves
Muscle injury
Platelet-rich plasma
Suture
Collagen
title_short Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma
title_full Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma
title_fullStr Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma
title_full_unstemmed Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma
title_sort Collagen I and III Ratios and Tenacity of Rats' Muscle Injured and Treated with Platelet-Rich Plasma
author Garcia, Thiago Alves
author_facet Garcia, Thiago Alves
Tamura Ozaki, Guilherme Akio [UNESP]
Castoldi, Robson Chacon
Job, Aldo Eloizo [UNESP]
Trindade Camargo, Regina Celi [UNESP]
Silva Camargo Filho, Jose Carlos [UNESP]
Belangero, William Dias
author_role author
author2 Tamura Ozaki, Guilherme Akio [UNESP]
Castoldi, Robson Chacon
Job, Aldo Eloizo [UNESP]
Trindade Camargo, Regina Celi [UNESP]
Silva Camargo Filho, Jose Carlos [UNESP]
Belangero, William Dias
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (Unesp)
Univ Oeste Paulista
dc.contributor.author.fl_str_mv Garcia, Thiago Alves
Tamura Ozaki, Guilherme Akio [UNESP]
Castoldi, Robson Chacon
Job, Aldo Eloizo [UNESP]
Trindade Camargo, Regina Celi [UNESP]
Silva Camargo Filho, Jose Carlos [UNESP]
Belangero, William Dias
dc.subject.por.fl_str_mv Muscle injury
Platelet-rich plasma
Suture
Collagen
topic Muscle injury
Platelet-rich plasma
Suture
Collagen
description Severe muscle injuries are common in accidents and have a delayed recovery of muscle integrity. In these cases, muscle suture surgery is the standard treatment. However, Platelet Rich Plasma (PRP), has been widely used in orthopedic injuries due to its growth factors. Thus, the objective of the study will be to analyze the association of suture and PRP techniques in the collagen and tenacity of the injured muscle. Were used seventy rats, divided into five groups: control (C), injury control (CI), injury and suture (IS), injury and PRP (IP), injury, suture, and PRP (ISP). Were sectioned approximately 50 % of the width and 100 % of the thickness of the gastrocnemius muscle. The homologous PRP was applied 24h after the injury. On the 7th day after the injury, the animals were euthanized and their muscles subjected to mechanical testing to measure tenacity or collagen analysis to calculate the ratio between type I and III collagen. The results show a significant decrease (p <0.05) in the values of the relationship between collagens in all injured groups (CI, IS, IP, ISP) compared to group C. In injured groups, the tenacity was significantly (p <0.05) reduced compared to the control group, with no observed difference between treatments and injured groups. The amount of collagen in the injured area has increased, but it did not affect the tenacity of the muscles, which was reduced.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-01
2021-06-25T12:18:32Z
2021-06-25T12:18:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv International Journal Of Morphology. Temuco: Soc Chilena Anatomia, v. 38, n. 5, p. 1392-1397, 2020.
0717-9502
http://hdl.handle.net/11449/209433
WOS:000566485800036
identifier_str_mv International Journal Of Morphology. Temuco: Soc Chilena Anatomia, v. 38, n. 5, p. 1392-1397, 2020.
0717-9502
WOS:000566485800036
url http://hdl.handle.net/11449/209433
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal Of Morphology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1392-1397
dc.publisher.none.fl_str_mv Soc Chilena Anatomia
publisher.none.fl_str_mv Soc Chilena Anatomia
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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