Coumarin production in Mikania glomerata callus

Detalhes bibliográficos
Autor(a) principal: Pereira, Ana Maria Soares [UNESP]
Data de Publicação: 1999
Outros Autores: França, S. C., Câmara, Francisco Luiz Araújo [UNESP]
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.actahort.org/books/502/502_59.htm
http://hdl.handle.net/11449/66001
Resumo: Cell cultures of Mikania glomerata Sprengel were established with leaf segments cultured on White medium supplemented with 1 mg/L BA and 3 mg/L NAA. Different types and concentrations of growth regulators were tested for callus maintenance. Determination of coumarin content was performed in HPLC using authentic coumarin standard. Growth regulator concentration affected biomass and coumarin accumulation. Cultures developed in semisolid medium containing both BA and NAA exhibited enhanced biomass production as well as coumarin accumulation. In the most favorable conditions tested, cells accumulated 25 μg/g of dry weight what is much inferior to the yield already reported in intact plants (5 mg/g of dry weight). However, results obtained so far suggest several alternatives for culture manipulation in order to optimize the productivity of coumarin by M. glomerata cultured cells.
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spelling Coumarin production in Mikania glomerata callusGuacoMedicinal plantMikania glomerataTissue cultureCell cultures of Mikania glomerata Sprengel were established with leaf segments cultured on White medium supplemented with 1 mg/L BA and 3 mg/L NAA. Different types and concentrations of growth regulators were tested for callus maintenance. Determination of coumarin content was performed in HPLC using authentic coumarin standard. Growth regulator concentration affected biomass and coumarin accumulation. Cultures developed in semisolid medium containing both BA and NAA exhibited enhanced biomass production as well as coumarin accumulation. In the most favorable conditions tested, cells accumulated 25 μg/g of dry weight what is much inferior to the yield already reported in intact plants (5 mg/g of dry weight). However, results obtained so far suggest several alternatives for culture manipulation in order to optimize the productivity of coumarin by M. glomerata cultured cells.Departamento de Biotecnologia Vegetal Universidade de Ribeirão Preto UNAERP 14096380, Ribeirão Preto, SPDepartamento de Horticultura Faculdade de Agronomia UNESP, Botucatu, SPDepartamento de Horticultura Faculdade de Agronomia UNESP, Botucatu, SPUNAERP 14096380Universidade Estadual Paulista (Unesp)Pereira, Ana Maria Soares [UNESP]França, S. C.Câmara, Francisco Luiz Araújo [UNESP]2014-05-27T11:19:50Z2014-05-27T11:19:50Z1999-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject357-361http://www.actahort.org/books/502/502_59.htmActa Horticulturae, v. 502, p. 357-361.0567-7572http://hdl.handle.net/11449/660012-s2.0-0008173520Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Horticulturae0,198info:eu-repo/semantics/openAccess2024-04-30T14:34:25Zoai:repositorio.unesp.br:11449/66001Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:31:41.220648Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Coumarin production in Mikania glomerata callus
title Coumarin production in Mikania glomerata callus
spellingShingle Coumarin production in Mikania glomerata callus
Pereira, Ana Maria Soares [UNESP]
Guaco
Medicinal plant
Mikania glomerata
Tissue culture
title_short Coumarin production in Mikania glomerata callus
title_full Coumarin production in Mikania glomerata callus
title_fullStr Coumarin production in Mikania glomerata callus
title_full_unstemmed Coumarin production in Mikania glomerata callus
title_sort Coumarin production in Mikania glomerata callus
author Pereira, Ana Maria Soares [UNESP]
author_facet Pereira, Ana Maria Soares [UNESP]
França, S. C.
Câmara, Francisco Luiz Araújo [UNESP]
author_role author
author2 França, S. C.
Câmara, Francisco Luiz Araújo [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv UNAERP 14096380
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Pereira, Ana Maria Soares [UNESP]
França, S. C.
Câmara, Francisco Luiz Araújo [UNESP]
dc.subject.por.fl_str_mv Guaco
Medicinal plant
Mikania glomerata
Tissue culture
topic Guaco
Medicinal plant
Mikania glomerata
Tissue culture
description Cell cultures of Mikania glomerata Sprengel were established with leaf segments cultured on White medium supplemented with 1 mg/L BA and 3 mg/L NAA. Different types and concentrations of growth regulators were tested for callus maintenance. Determination of coumarin content was performed in HPLC using authentic coumarin standard. Growth regulator concentration affected biomass and coumarin accumulation. Cultures developed in semisolid medium containing both BA and NAA exhibited enhanced biomass production as well as coumarin accumulation. In the most favorable conditions tested, cells accumulated 25 μg/g of dry weight what is much inferior to the yield already reported in intact plants (5 mg/g of dry weight). However, results obtained so far suggest several alternatives for culture manipulation in order to optimize the productivity of coumarin by M. glomerata cultured cells.
publishDate 1999
dc.date.none.fl_str_mv 1999-12-01
2014-05-27T11:19:50Z
2014-05-27T11:19:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.actahort.org/books/502/502_59.htm
Acta Horticulturae, v. 502, p. 357-361.
0567-7572
http://hdl.handle.net/11449/66001
2-s2.0-0008173520
url http://www.actahort.org/books/502/502_59.htm
http://hdl.handle.net/11449/66001
identifier_str_mv Acta Horticulturae, v. 502, p. 357-361.
0567-7572
2-s2.0-0008173520
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Horticulturae
0,198
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 357-361
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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