Short Communication: Human Bone Marrow Stromal Cells Exhibit Immunosuppressive Effects on Human T Lymphotropic Virus Type 1 T Lymphocyte from Infected Individuals
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1089/aid.2018.0066 http://hdl.handle.net/11449/187311 |
Resumo: | Human multipotent mesenchymal stromal cells (MSCs) display immunoregulatory functions that can modulate innate and adaptive cellular immune responses. The suppressive and immunomodulatory activities of MSCs occur through the action of soluble factors that are constitutively produced and released by these cells or, alternatively, after MSC induction by stimuli of inflammatory microenvironments. However, to date the contribution of MSCs in the inflammatory microenvironment resulting from viral infection is unknown. In our study, we evaluated the MSC immunosuppressive effect on human T lymphotropic virus type 1 (HTLV-1) infected T lymphocytes. To evaluate if MSC immunoregulation can influence the proliferation of HTLV-1 infected T lymphocytes, we compared the proliferation of lymphocytes obtained from HTLV-1 infected and healthy individuals cocultured in the presence of MSCs. It was observed that the lymphoproliferative inhibition by MSCs on infected lymphocytes was similar compared to the cells obtained from healthy individuals. In addition, this suppressive effect was related to a significant increase of indoleamine-2,3-dioxygenase and prostaglandin E2 gene expression (p ≤.05). Furthermore, the HTLV-1 pol gene was less expressed after coculturing with MSCs, suggesting that the MSC immunoregulation can have effective suppression on HTLV-1 infected T cells. In conclusion, this study suggests that MSCs could be involved in the immunomodulation of the HTLV-1 infected T lymphocytes. |
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Short Communication: Human Bone Marrow Stromal Cells Exhibit Immunosuppressive Effects on Human T Lymphotropic Virus Type 1 T Lymphocyte from Infected Individualsgene expressionHTLV-1immunoregulationMSCHuman multipotent mesenchymal stromal cells (MSCs) display immunoregulatory functions that can modulate innate and adaptive cellular immune responses. The suppressive and immunomodulatory activities of MSCs occur through the action of soluble factors that are constitutively produced and released by these cells or, alternatively, after MSC induction by stimuli of inflammatory microenvironments. However, to date the contribution of MSCs in the inflammatory microenvironment resulting from viral infection is unknown. In our study, we evaluated the MSC immunosuppressive effect on human T lymphotropic virus type 1 (HTLV-1) infected T lymphocytes. To evaluate if MSC immunoregulation can influence the proliferation of HTLV-1 infected T lymphocytes, we compared the proliferation of lymphocytes obtained from HTLV-1 infected and healthy individuals cocultured in the presence of MSCs. It was observed that the lymphoproliferative inhibition by MSCs on infected lymphocytes was similar compared to the cells obtained from healthy individuals. In addition, this suppressive effect was related to a significant increase of indoleamine-2,3-dioxygenase and prostaglandin E2 gene expression (p ≤.05). Furthermore, the HTLV-1 pol gene was less expressed after coculturing with MSCs, suggesting that the MSC immunoregulation can have effective suppression on HTLV-1 infected T cells. In conclusion, this study suggests that MSCs could be involved in the immunomodulation of the HTLV-1 infected T lymphocytes.Center for Cell-Based Research Regional Blood Center of Ribeirão Preto School of Medicine of Ribeirão Preto University of São Paulo (USP), Avenida Tenente Catao Roxo Ribeirao PretoDepartment of Clinical Toxicological and Bromatological Analysis Faculty of Pharmaceutical Sciences of Ribeirão PretoDepartment of Clinical Medicine School of Medicine of Ribeirão Preto University of São Paulo (USP)São Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Sciences (IBILCE)São Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Sciences (IBILCE)Universidade de São Paulo (USP)Faculty of Pharmaceutical Sciences of Ribeirão PretoUniversidade Estadual Paulista (Unesp)Rodrigues, Evandra StrazzaDe MacEdo, Mayra DoriganOrellana, Maristela DelgadoTakayanagui, Osvaldo MassaitiPalma, Patrícia Vianna BoniniPinto, Mariana TomaziniDe Oliveira, Gislane Lelis Vilela [UNESP]Malmegrim, Kelen Cristina RibeiroSlavov, Svetoslav NanevCovas, Dimas TadeuKashima, Simone2019-10-06T15:32:15Z2019-10-06T15:32:15Z2019-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article164-168http://dx.doi.org/10.1089/aid.2018.0066AIDS Research and Human Retroviruses, v. 35, n. 2, p. 164-168, 2019.1931-84050889-2229http://hdl.handle.net/11449/18731110.1089/aid.2018.00662-s2.0-85060913943Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAIDS Research and Human Retrovirusesinfo:eu-repo/semantics/openAccess2021-10-22T18:20:44Zoai:repositorio.unesp.br:11449/187311Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T18:20:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Short Communication: Human Bone Marrow Stromal Cells Exhibit Immunosuppressive Effects on Human T Lymphotropic Virus Type 1 T Lymphocyte from Infected Individuals |
title |
Short Communication: Human Bone Marrow Stromal Cells Exhibit Immunosuppressive Effects on Human T Lymphotropic Virus Type 1 T Lymphocyte from Infected Individuals |
spellingShingle |
Short Communication: Human Bone Marrow Stromal Cells Exhibit Immunosuppressive Effects on Human T Lymphotropic Virus Type 1 T Lymphocyte from Infected Individuals Rodrigues, Evandra Strazza gene expression HTLV-1 immunoregulation MSC |
title_short |
Short Communication: Human Bone Marrow Stromal Cells Exhibit Immunosuppressive Effects on Human T Lymphotropic Virus Type 1 T Lymphocyte from Infected Individuals |
title_full |
Short Communication: Human Bone Marrow Stromal Cells Exhibit Immunosuppressive Effects on Human T Lymphotropic Virus Type 1 T Lymphocyte from Infected Individuals |
title_fullStr |
Short Communication: Human Bone Marrow Stromal Cells Exhibit Immunosuppressive Effects on Human T Lymphotropic Virus Type 1 T Lymphocyte from Infected Individuals |
title_full_unstemmed |
Short Communication: Human Bone Marrow Stromal Cells Exhibit Immunosuppressive Effects on Human T Lymphotropic Virus Type 1 T Lymphocyte from Infected Individuals |
title_sort |
Short Communication: Human Bone Marrow Stromal Cells Exhibit Immunosuppressive Effects on Human T Lymphotropic Virus Type 1 T Lymphocyte from Infected Individuals |
author |
Rodrigues, Evandra Strazza |
author_facet |
Rodrigues, Evandra Strazza De MacEdo, Mayra Dorigan Orellana, Maristela Delgado Takayanagui, Osvaldo Massaiti Palma, Patrícia Vianna Bonini Pinto, Mariana Tomazini De Oliveira, Gislane Lelis Vilela [UNESP] Malmegrim, Kelen Cristina Ribeiro Slavov, Svetoslav Nanev Covas, Dimas Tadeu Kashima, Simone |
author_role |
author |
author2 |
De MacEdo, Mayra Dorigan Orellana, Maristela Delgado Takayanagui, Osvaldo Massaiti Palma, Patrícia Vianna Bonini Pinto, Mariana Tomazini De Oliveira, Gislane Lelis Vilela [UNESP] Malmegrim, Kelen Cristina Ribeiro Slavov, Svetoslav Nanev Covas, Dimas Tadeu Kashima, Simone |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Faculty of Pharmaceutical Sciences of Ribeirão Preto Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rodrigues, Evandra Strazza De MacEdo, Mayra Dorigan Orellana, Maristela Delgado Takayanagui, Osvaldo Massaiti Palma, Patrícia Vianna Bonini Pinto, Mariana Tomazini De Oliveira, Gislane Lelis Vilela [UNESP] Malmegrim, Kelen Cristina Ribeiro Slavov, Svetoslav Nanev Covas, Dimas Tadeu Kashima, Simone |
dc.subject.por.fl_str_mv |
gene expression HTLV-1 immunoregulation MSC |
topic |
gene expression HTLV-1 immunoregulation MSC |
description |
Human multipotent mesenchymal stromal cells (MSCs) display immunoregulatory functions that can modulate innate and adaptive cellular immune responses. The suppressive and immunomodulatory activities of MSCs occur through the action of soluble factors that are constitutively produced and released by these cells or, alternatively, after MSC induction by stimuli of inflammatory microenvironments. However, to date the contribution of MSCs in the inflammatory microenvironment resulting from viral infection is unknown. In our study, we evaluated the MSC immunosuppressive effect on human T lymphotropic virus type 1 (HTLV-1) infected T lymphocytes. To evaluate if MSC immunoregulation can influence the proliferation of HTLV-1 infected T lymphocytes, we compared the proliferation of lymphocytes obtained from HTLV-1 infected and healthy individuals cocultured in the presence of MSCs. It was observed that the lymphoproliferative inhibition by MSCs on infected lymphocytes was similar compared to the cells obtained from healthy individuals. In addition, this suppressive effect was related to a significant increase of indoleamine-2,3-dioxygenase and prostaglandin E2 gene expression (p ≤.05). Furthermore, the HTLV-1 pol gene was less expressed after coculturing with MSCs, suggesting that the MSC immunoregulation can have effective suppression on HTLV-1 infected T cells. In conclusion, this study suggests that MSCs could be involved in the immunomodulation of the HTLV-1 infected T lymphocytes. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:32:15Z 2019-10-06T15:32:15Z 2019-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1089/aid.2018.0066 AIDS Research and Human Retroviruses, v. 35, n. 2, p. 164-168, 2019. 1931-8405 0889-2229 http://hdl.handle.net/11449/187311 10.1089/aid.2018.0066 2-s2.0-85060913943 |
url |
http://dx.doi.org/10.1089/aid.2018.0066 http://hdl.handle.net/11449/187311 |
identifier_str_mv |
AIDS Research and Human Retroviruses, v. 35, n. 2, p. 164-168, 2019. 1931-8405 0889-2229 10.1089/aid.2018.0066 2-s2.0-85060913943 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
AIDS Research and Human Retroviruses |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
164-168 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047229871620096 |