Effects of low-level fluoride exposure on glucose homeostasis in female NOD mice
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.chemosphere.2020.126602 http://hdl.handle.net/11449/196951 |
Resumo: | Water fluoridation is an important public health measure for the control of dental caries. Recent animal studies have shown that low doses of fluoride (F) in the drinking water, similar to those found in public water supplies, increase insulin sensitivity and reduce blood glucose. In the present study we evaluated the effects of low-level F exposure through the drinking water on glucose homeostasis in female NOD mice. Seventy-two 6-week mice were randomly divided into 2 groups according to the concentration of F in the drinking water (0-control, or 10 mg/L) they received for 14 weeks. After the experimental period the blood was collected for analyses of plasma F, glucose and insulin. Liver and gastrocnemius muscle were collected for proteomic analysis. Plasma F concentrations were significantly higher in the F-treated than in the control group. Despite treatment with fluoridated water reduced plasma levels glucose by 20% compared to control, no significant differences were found between the groups for plasma glucose and insulin. In the muscle, treatment with fluoridated water increased the expression of proteins related to muscle contraction, while in the liver, there was an increase in expression of antioxidant proteins and in proteins related to carboxylic acid metabolic process. Remarkably, phosphoenolpyruvate carboxy-kinase (PEPCK) was found exclusively in the liver of control mice. The reduction in PEPCK, a positive regulator of gluconeogenesis, thus increasing glucose uptake, might be a probable mechanism to explain the anti-diabetic effects of low doses of F, which should be evaluated in further studies. (C) 2020 Elsevier Ltd. All rights reserved. |
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Effects of low-level fluoride exposure on glucose homeostasis in female NOD miceFluorideSensitivity in insulinGlucoseMice NODWater fluoridation is an important public health measure for the control of dental caries. Recent animal studies have shown that low doses of fluoride (F) in the drinking water, similar to those found in public water supplies, increase insulin sensitivity and reduce blood glucose. In the present study we evaluated the effects of low-level F exposure through the drinking water on glucose homeostasis in female NOD mice. Seventy-two 6-week mice were randomly divided into 2 groups according to the concentration of F in the drinking water (0-control, or 10 mg/L) they received for 14 weeks. After the experimental period the blood was collected for analyses of plasma F, glucose and insulin. Liver and gastrocnemius muscle were collected for proteomic analysis. Plasma F concentrations were significantly higher in the F-treated than in the control group. Despite treatment with fluoridated water reduced plasma levels glucose by 20% compared to control, no significant differences were found between the groups for plasma glucose and insulin. In the muscle, treatment with fluoridated water increased the expression of proteins related to muscle contraction, while in the liver, there was an increase in expression of antioxidant proteins and in proteins related to carboxylic acid metabolic process. Remarkably, phosphoenolpyruvate carboxy-kinase (PEPCK) was found exclusively in the liver of control mice. The reduction in PEPCK, a positive regulator of gluconeogenesis, thus increasing glucose uptake, might be a probable mechanism to explain the anti-diabetic effects of low doses of F, which should be evaluated in further studies. (C) 2020 Elsevier Ltd. All rights reserved.Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP, BrazilSao Paulo State Univ, Inst Biosci, Bauru, SP, BrazilSao Paulo State Univ, Fac Sci, Dept Phys Educ, Bauru, SP, BrazilSao Paulo State Univ, Inst Biosci, Bauru, SP, BrazilSao Paulo State Univ, Fac Sci, Dept Phys Educ, Bauru, SP, BrazilElsevier B.V.Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Trevizol, Juliana SanchesBuzalaf, Nathalia RabeloDionizio, AlineDelgado, Aislan Quintiliano [UNESP]Cestari, Tania MaryBosqueiro, Jose Roberto [UNESP]Magalhaes, Ana CarolinaRabelo Buzalaf, Marilia Afonso2020-12-10T20:01:25Z2020-12-10T20:01:25Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7http://dx.doi.org/10.1016/j.chemosphere.2020.126602Chemosphere. Oxford: Pergamon-elsevier Science Ltd, v. 254, 7 p., 2020.0045-6535http://hdl.handle.net/11449/19695110.1016/j.chemosphere.2020.126602WOS:000538150400001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosphereinfo:eu-repo/semantics/openAccess2024-04-24T18:53:20Zoai:repositorio.unesp.br:11449/196951Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:11:53.942133Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of low-level fluoride exposure on glucose homeostasis in female NOD mice |
title |
Effects of low-level fluoride exposure on glucose homeostasis in female NOD mice |
spellingShingle |
Effects of low-level fluoride exposure on glucose homeostasis in female NOD mice Trevizol, Juliana Sanches Fluoride Sensitivity in insulin Glucose Mice NOD |
title_short |
Effects of low-level fluoride exposure on glucose homeostasis in female NOD mice |
title_full |
Effects of low-level fluoride exposure on glucose homeostasis in female NOD mice |
title_fullStr |
Effects of low-level fluoride exposure on glucose homeostasis in female NOD mice |
title_full_unstemmed |
Effects of low-level fluoride exposure on glucose homeostasis in female NOD mice |
title_sort |
Effects of low-level fluoride exposure on glucose homeostasis in female NOD mice |
author |
Trevizol, Juliana Sanches |
author_facet |
Trevizol, Juliana Sanches Buzalaf, Nathalia Rabelo Dionizio, Aline Delgado, Aislan Quintiliano [UNESP] Cestari, Tania Mary Bosqueiro, Jose Roberto [UNESP] Magalhaes, Ana Carolina Rabelo Buzalaf, Marilia Afonso |
author_role |
author |
author2 |
Buzalaf, Nathalia Rabelo Dionizio, Aline Delgado, Aislan Quintiliano [UNESP] Cestari, Tania Mary Bosqueiro, Jose Roberto [UNESP] Magalhaes, Ana Carolina Rabelo Buzalaf, Marilia Afonso |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Trevizol, Juliana Sanches Buzalaf, Nathalia Rabelo Dionizio, Aline Delgado, Aislan Quintiliano [UNESP] Cestari, Tania Mary Bosqueiro, Jose Roberto [UNESP] Magalhaes, Ana Carolina Rabelo Buzalaf, Marilia Afonso |
dc.subject.por.fl_str_mv |
Fluoride Sensitivity in insulin Glucose Mice NOD |
topic |
Fluoride Sensitivity in insulin Glucose Mice NOD |
description |
Water fluoridation is an important public health measure for the control of dental caries. Recent animal studies have shown that low doses of fluoride (F) in the drinking water, similar to those found in public water supplies, increase insulin sensitivity and reduce blood glucose. In the present study we evaluated the effects of low-level F exposure through the drinking water on glucose homeostasis in female NOD mice. Seventy-two 6-week mice were randomly divided into 2 groups according to the concentration of F in the drinking water (0-control, or 10 mg/L) they received for 14 weeks. After the experimental period the blood was collected for analyses of plasma F, glucose and insulin. Liver and gastrocnemius muscle were collected for proteomic analysis. Plasma F concentrations were significantly higher in the F-treated than in the control group. Despite treatment with fluoridated water reduced plasma levels glucose by 20% compared to control, no significant differences were found between the groups for plasma glucose and insulin. In the muscle, treatment with fluoridated water increased the expression of proteins related to muscle contraction, while in the liver, there was an increase in expression of antioxidant proteins and in proteins related to carboxylic acid metabolic process. Remarkably, phosphoenolpyruvate carboxy-kinase (PEPCK) was found exclusively in the liver of control mice. The reduction in PEPCK, a positive regulator of gluconeogenesis, thus increasing glucose uptake, might be a probable mechanism to explain the anti-diabetic effects of low doses of F, which should be evaluated in further studies. (C) 2020 Elsevier Ltd. All rights reserved. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T20:01:25Z 2020-12-10T20:01:25Z 2020-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.chemosphere.2020.126602 Chemosphere. Oxford: Pergamon-elsevier Science Ltd, v. 254, 7 p., 2020. 0045-6535 http://hdl.handle.net/11449/196951 10.1016/j.chemosphere.2020.126602 WOS:000538150400001 |
url |
http://dx.doi.org/10.1016/j.chemosphere.2020.126602 http://hdl.handle.net/11449/196951 |
identifier_str_mv |
Chemosphere. Oxford: Pergamon-elsevier Science Ltd, v. 254, 7 p., 2020. 0045-6535 10.1016/j.chemosphere.2020.126602 WOS:000538150400001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemosphere |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128907094786048 |