Development of a protocol to assess cell internalization and tissue uptake of magnetic nanoparticles by AC Biosusceptometry
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jmmm.2018.10.064 http://hdl.handle.net/11449/187062 |
Resumo: | Several applications of nanoparticles rely on the internalization and accumulation of nanocarriers in specific cell compartments and tissues. However, the methods currently employed for characterizing such processes, although well described, are time consuming and do not provide in vivo information, which is a crucial barrier towards translational applications. Here, we hypothesize that the AC Biosusceptometry technique can be employed to assess cell internalization of magnetic nanoparticles, with possible applications in screening assays to track specific biomarkers and cell types. We tested a simpler and easier alternative to study cell internalization and tissue accumulation after perfusion. We utilized citrate coated, manganese ferrite nanoparticles and evaluated the internalization process in mouse macrophages cells (J774.A1) and in an embryonic neural stem cell culture (E14.5) after differentiation in astrocytes and neurons, to assess internalization specificity. Respecting the particles toxicity limits, we tested different concentration of particles, in different incubation times. Sequentially, we imaged the cell cultures to confirm internalization and nanoparticles localization, labeling nucleus and cell body to assure that the particles were inside the cells. Our results showed a linear behavior on internalization for different doses and an optimum incubation time of 2 h. |
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Development of a protocol to assess cell internalization and tissue uptake of magnetic nanoparticles by AC BiosusceptometrySeveral applications of nanoparticles rely on the internalization and accumulation of nanocarriers in specific cell compartments and tissues. However, the methods currently employed for characterizing such processes, although well described, are time consuming and do not provide in vivo information, which is a crucial barrier towards translational applications. Here, we hypothesize that the AC Biosusceptometry technique can be employed to assess cell internalization of magnetic nanoparticles, with possible applications in screening assays to track specific biomarkers and cell types. We tested a simpler and easier alternative to study cell internalization and tissue accumulation after perfusion. We utilized citrate coated, manganese ferrite nanoparticles and evaluated the internalization process in mouse macrophages cells (J774.A1) and in an embryonic neural stem cell culture (E14.5) after differentiation in astrocytes and neurons, to assess internalization specificity. Respecting the particles toxicity limits, we tested different concentration of particles, in different incubation times. Sequentially, we imaged the cell cultures to confirm internalization and nanoparticles localization, labeling nucleus and cell body to assure that the particles were inside the cells. Our results showed a linear behavior on internalization for different doses and an optimum incubation time of 2 h.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Física e Biofísica Institute of Biosciences Sao Paulo State University (Unesp)Center for Neuroregeneration Houston Methodist Research InstituteDepartamento de Física Faculdade de Física Ciências e Letras de Ribeirão Preto University of Sao Paulo (USP)Instituto de Física Federal University of GoiásDepartamento de Física e Biofísica Institute of Biosciences Sao Paulo State University (Unesp)FAPESP: 2015/14914-0FAPESP: 2017/00842-2Universidade Estadual Paulista (Unesp)Houston Methodist Research InstituteUniversidade de São Paulo (USP)Universidade Federal de Goiás (UFG)Quini, Caio C. [UNESP]Próspero, André G. [UNESP]Kondiles, Bethany R.Chaboub, LesleyHogan, Matthew K.Baffa, OswaldoBakuzis, Andris F.Horner, Philip J.Miranda, José R.A. [UNESP]2019-10-06T15:24:22Z2019-10-06T15:24:22Z2019-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article527-533http://dx.doi.org/10.1016/j.jmmm.2018.10.064Journal of Magnetism and Magnetic Materials, v. 473, p. 527-533.0304-8853http://hdl.handle.net/11449/18706210.1016/j.jmmm.2018.10.0642-s2.0-85056565340Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Magnetism and Magnetic Materialsinfo:eu-repo/semantics/openAccess2021-10-23T02:54:00Zoai:repositorio.unesp.br:11449/187062Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:32:48.063997Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development of a protocol to assess cell internalization and tissue uptake of magnetic nanoparticles by AC Biosusceptometry |
title |
Development of a protocol to assess cell internalization and tissue uptake of magnetic nanoparticles by AC Biosusceptometry |
spellingShingle |
Development of a protocol to assess cell internalization and tissue uptake of magnetic nanoparticles by AC Biosusceptometry Quini, Caio C. [UNESP] |
title_short |
Development of a protocol to assess cell internalization and tissue uptake of magnetic nanoparticles by AC Biosusceptometry |
title_full |
Development of a protocol to assess cell internalization and tissue uptake of magnetic nanoparticles by AC Biosusceptometry |
title_fullStr |
Development of a protocol to assess cell internalization and tissue uptake of magnetic nanoparticles by AC Biosusceptometry |
title_full_unstemmed |
Development of a protocol to assess cell internalization and tissue uptake of magnetic nanoparticles by AC Biosusceptometry |
title_sort |
Development of a protocol to assess cell internalization and tissue uptake of magnetic nanoparticles by AC Biosusceptometry |
author |
Quini, Caio C. [UNESP] |
author_facet |
Quini, Caio C. [UNESP] Próspero, André G. [UNESP] Kondiles, Bethany R. Chaboub, Lesley Hogan, Matthew K. Baffa, Oswaldo Bakuzis, Andris F. Horner, Philip J. Miranda, José R.A. [UNESP] |
author_role |
author |
author2 |
Próspero, André G. [UNESP] Kondiles, Bethany R. Chaboub, Lesley Hogan, Matthew K. Baffa, Oswaldo Bakuzis, Andris F. Horner, Philip J. Miranda, José R.A. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Houston Methodist Research Institute Universidade de São Paulo (USP) Universidade Federal de Goiás (UFG) |
dc.contributor.author.fl_str_mv |
Quini, Caio C. [UNESP] Próspero, André G. [UNESP] Kondiles, Bethany R. Chaboub, Lesley Hogan, Matthew K. Baffa, Oswaldo Bakuzis, Andris F. Horner, Philip J. Miranda, José R.A. [UNESP] |
description |
Several applications of nanoparticles rely on the internalization and accumulation of nanocarriers in specific cell compartments and tissues. However, the methods currently employed for characterizing such processes, although well described, are time consuming and do not provide in vivo information, which is a crucial barrier towards translational applications. Here, we hypothesize that the AC Biosusceptometry technique can be employed to assess cell internalization of magnetic nanoparticles, with possible applications in screening assays to track specific biomarkers and cell types. We tested a simpler and easier alternative to study cell internalization and tissue accumulation after perfusion. We utilized citrate coated, manganese ferrite nanoparticles and evaluated the internalization process in mouse macrophages cells (J774.A1) and in an embryonic neural stem cell culture (E14.5) after differentiation in astrocytes and neurons, to assess internalization specificity. Respecting the particles toxicity limits, we tested different concentration of particles, in different incubation times. Sequentially, we imaged the cell cultures to confirm internalization and nanoparticles localization, labeling nucleus and cell body to assure that the particles were inside the cells. Our results showed a linear behavior on internalization for different doses and an optimum incubation time of 2 h. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:24:22Z 2019-10-06T15:24:22Z 2019-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jmmm.2018.10.064 Journal of Magnetism and Magnetic Materials, v. 473, p. 527-533. 0304-8853 http://hdl.handle.net/11449/187062 10.1016/j.jmmm.2018.10.064 2-s2.0-85056565340 |
url |
http://dx.doi.org/10.1016/j.jmmm.2018.10.064 http://hdl.handle.net/11449/187062 |
identifier_str_mv |
Journal of Magnetism and Magnetic Materials, v. 473, p. 527-533. 0304-8853 10.1016/j.jmmm.2018.10.064 2-s2.0-85056565340 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Magnetism and Magnetic Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
527-533 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128669279846400 |