Typing class I HLA-A gene using a nested PCR-RFLP procedure

Detalhes bibliográficos
Autor(a) principal: Castelli, E.C. [UNESP]
Data de Publicação: 2005
Outros Autores: Gil, D.S. [UNESP], Veiga, L.C.S., de Camargo, J.L.V. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-879X2005000600004
http://hdl.handle.net/11449/12834
Resumo: In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
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spelling Typing class I HLA-A gene using a nested PCR-RFLP procedureHuman leukocyte antigen-AHLA-ATypingRFLPPolymorphismMajor histocompatibility complexIn order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.Universidade Estadual Paulista Faculdade de Medicina Departamento de PatologiaUniversidade de São Paulo Faculdade de Medicina de Ribeirão Preto Departamento de GenéticaUniversidade Estadual Paulista Faculdade de Medicina Departamento de PatologiaAssociação Brasileira de Divulgação Científica (ABRADIC)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Castelli, E.C. [UNESP]Gil, D.S. [UNESP]Veiga, L.C.S.de Camargo, J.L.V. [UNESP]2014-05-20T13:37:08Z2014-05-20T13:37:08Z2005-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article837-842application/pdfhttp://dx.doi.org/10.1590/S0100-879X2005000600004Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 6, p. 837-842, 2005.0100-879Xhttp://hdl.handle.net/11449/1283410.1590/S0100-879X2005000600004S0100-879X2005000600004S0100-879X2005000600004.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Research1.492info:eu-repo/semantics/openAccess2024-09-03T13:14:11Zoai:repositorio.unesp.br:11449/12834Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Typing class I HLA-A gene using a nested PCR-RFLP procedure
title Typing class I HLA-A gene using a nested PCR-RFLP procedure
spellingShingle Typing class I HLA-A gene using a nested PCR-RFLP procedure
Castelli, E.C. [UNESP]
Human leukocyte antigen-A
HLA-A
Typing
RFLP
Polymorphism
Major histocompatibility complex
title_short Typing class I HLA-A gene using a nested PCR-RFLP procedure
title_full Typing class I HLA-A gene using a nested PCR-RFLP procedure
title_fullStr Typing class I HLA-A gene using a nested PCR-RFLP procedure
title_full_unstemmed Typing class I HLA-A gene using a nested PCR-RFLP procedure
title_sort Typing class I HLA-A gene using a nested PCR-RFLP procedure
author Castelli, E.C. [UNESP]
author_facet Castelli, E.C. [UNESP]
Gil, D.S. [UNESP]
Veiga, L.C.S.
de Camargo, J.L.V. [UNESP]
author_role author
author2 Gil, D.S. [UNESP]
Veiga, L.C.S.
de Camargo, J.L.V. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Castelli, E.C. [UNESP]
Gil, D.S. [UNESP]
Veiga, L.C.S.
de Camargo, J.L.V. [UNESP]
dc.subject.por.fl_str_mv Human leukocyte antigen-A
HLA-A
Typing
RFLP
Polymorphism
Major histocompatibility complex
topic Human leukocyte antigen-A
HLA-A
Typing
RFLP
Polymorphism
Major histocompatibility complex
description In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
publishDate 2005
dc.date.none.fl_str_mv 2005-06-01
2014-05-20T13:37:08Z
2014-05-20T13:37:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2005000600004
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 6, p. 837-842, 2005.
0100-879X
http://hdl.handle.net/11449/12834
10.1590/S0100-879X2005000600004
S0100-879X2005000600004
S0100-879X2005000600004.pdf
url http://dx.doi.org/10.1590/S0100-879X2005000600004
http://hdl.handle.net/11449/12834
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 6, p. 837-842, 2005.
0100-879X
10.1590/S0100-879X2005000600004
S0100-879X2005000600004
S0100-879X2005000600004.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
1.492
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 837-842
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica (ABRADIC)
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica (ABRADIC)
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1810021356501204992

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