Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips

Detalhes bibliográficos
Autor(a) principal: Matsumoto, Silvia Tamie
Data de Publicação: 2006
Outros Autores: Mantovani, Mário Sérgio, Malaguttii, Mirtis Irene Ariza [UNESP], Dias, Ana Lúcia, Fonseca, Inês Cristina, Marin-Morales, Maria Aparecida [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1415-47572006000100028
http://hdl.handle.net/11449/30482
Resumo: Cytotoxicity of metals is important because some metals are potential mutagens able to induce tumors in humans and experimental animals. Chromium can damage DNA in several ways, including DNA double strand breaks (DSBs) which generate chromosomal aberrations, micronucleus formation, sister chromatid exchange, formation of DNA adducts and alterations in DNA replication and transcription. In our study, water samples from three sites in the Córrego dos Bagres stream in the Franca municipality of the Brazilian state of São Paulo were subjected to the comet assay and micronucleus test using erythrocytes from the fish Oreochromis niloticus. Nuclear abnormalities of the erythrocytes included blebbed, notched and lobed nuclei, probably due to genotoxic chromium compounds. The greatest comet assay damage occurred with water from a chromium-containing tannery effluent discharge site, supporting the hypothesis that chromium residues can be genotoxic. The mutagenicity of the water samples was assessed using the onion root-tip cell assay, the most frequent chromosomal abnormalities observed being: c-metaphases, stick chromosome, chromosome breaks and losses, bridged anaphases, multipolar anaphases, and micronucleated and binucleated cells. Onion root-tip cell mutagenicity was highest for water samples containing the highest levels of chromium.
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spelling Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tipsAllium cepachromiumchromosomes aberrationscomet assaymicronucleusOreochromis niloticusCytotoxicity of metals is important because some metals are potential mutagens able to induce tumors in humans and experimental animals. Chromium can damage DNA in several ways, including DNA double strand breaks (DSBs) which generate chromosomal aberrations, micronucleus formation, sister chromatid exchange, formation of DNA adducts and alterations in DNA replication and transcription. In our study, water samples from three sites in the Córrego dos Bagres stream in the Franca municipality of the Brazilian state of São Paulo were subjected to the comet assay and micronucleus test using erythrocytes from the fish Oreochromis niloticus. Nuclear abnormalities of the erythrocytes included blebbed, notched and lobed nuclei, probably due to genotoxic chromium compounds. The greatest comet assay damage occurred with water from a chromium-containing tannery effluent discharge site, supporting the hypothesis that chromium residues can be genotoxic. The mutagenicity of the water samples was assessed using the onion root-tip cell assay, the most frequent chromosomal abnormalities observed being: c-metaphases, stick chromosome, chromosome breaks and losses, bridged anaphases, multipolar anaphases, and micronucleated and binucleated cells. Onion root-tip cell mutagenicity was highest for water samples containing the highest levels of chromium.Universidade Federal do Espírito Santo (UFES) Departamento de Ciências BiológicasUniversidade Estadual de Londrina (UEL) Departamento de Biologia GeralUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Geociências e Ciências Exatas Departamento de Petrologia e MetalogeniaUniversidade Estadual de Londrina (UEL) Departamento de AgronomiaUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Departamento de BiologiaUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Geociências e Ciências Exatas Departamento de Petrologia e MetalogeniaUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Departamento de BiologiaSociedade Brasileira de GenéticaUniversidade Federal do Espírito Santo (UFES)Universidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)Matsumoto, Silvia TamieMantovani, Mário SérgioMalaguttii, Mirtis Irene Ariza [UNESP]Dias, Ana LúciaFonseca, Inês CristinaMarin-Morales, Maria Aparecida [UNESP]2014-05-20T15:17:28Z2014-05-20T15:17:28Z2006-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article148-158application/pdfhttp://dx.doi.org/10.1590/S1415-47572006000100028Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 29, n. 1, p. 148-158, 2006.1415-4757http://hdl.handle.net/11449/3048210.1590/S1415-47572006000100028S1415-47572006000100028S1415-47572006000100028.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-12-22T06:25:57Zoai:repositorio.unesp.br:11449/30482Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-22T06:25:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips
title Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips
spellingShingle Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips
Matsumoto, Silvia Tamie
Allium cepa
chromium
chromosomes aberrations
comet assay
micronucleus
Oreochromis niloticus
title_short Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips
title_full Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips
title_fullStr Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips
title_full_unstemmed Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips
title_sort Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips
author Matsumoto, Silvia Tamie
author_facet Matsumoto, Silvia Tamie
Mantovani, Mário Sérgio
Malaguttii, Mirtis Irene Ariza [UNESP]
Dias, Ana Lúcia
Fonseca, Inês Cristina
Marin-Morales, Maria Aparecida [UNESP]
author_role author
author2 Mantovani, Mário Sérgio
Malaguttii, Mirtis Irene Ariza [UNESP]
Dias, Ana Lúcia
Fonseca, Inês Cristina
Marin-Morales, Maria Aparecida [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do Espírito Santo (UFES)
Universidade Estadual de Londrina (UEL)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Matsumoto, Silvia Tamie
Mantovani, Mário Sérgio
Malaguttii, Mirtis Irene Ariza [UNESP]
Dias, Ana Lúcia
Fonseca, Inês Cristina
Marin-Morales, Maria Aparecida [UNESP]
dc.subject.por.fl_str_mv Allium cepa
chromium
chromosomes aberrations
comet assay
micronucleus
Oreochromis niloticus
topic Allium cepa
chromium
chromosomes aberrations
comet assay
micronucleus
Oreochromis niloticus
description Cytotoxicity of metals is important because some metals are potential mutagens able to induce tumors in humans and experimental animals. Chromium can damage DNA in several ways, including DNA double strand breaks (DSBs) which generate chromosomal aberrations, micronucleus formation, sister chromatid exchange, formation of DNA adducts and alterations in DNA replication and transcription. In our study, water samples from three sites in the Córrego dos Bagres stream in the Franca municipality of the Brazilian state of São Paulo were subjected to the comet assay and micronucleus test using erythrocytes from the fish Oreochromis niloticus. Nuclear abnormalities of the erythrocytes included blebbed, notched and lobed nuclei, probably due to genotoxic chromium compounds. The greatest comet assay damage occurred with water from a chromium-containing tannery effluent discharge site, supporting the hypothesis that chromium residues can be genotoxic. The mutagenicity of the water samples was assessed using the onion root-tip cell assay, the most frequent chromosomal abnormalities observed being: c-metaphases, stick chromosome, chromosome breaks and losses, bridged anaphases, multipolar anaphases, and micronucleated and binucleated cells. Onion root-tip cell mutagenicity was highest for water samples containing the highest levels of chromium.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01
2014-05-20T15:17:28Z
2014-05-20T15:17:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572006000100028
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 29, n. 1, p. 148-158, 2006.
1415-4757
http://hdl.handle.net/11449/30482
10.1590/S1415-47572006000100028
S1415-47572006000100028
S1415-47572006000100028.pdf
url http://dx.doi.org/10.1590/S1415-47572006000100028
http://hdl.handle.net/11449/30482
identifier_str_mv Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 29, n. 1, p. 148-158, 2006.
1415-4757
10.1590/S1415-47572006000100028
S1415-47572006000100028
S1415-47572006000100028.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
1.493
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 148-158
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1803047067818393600

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