Genomics and proteomics approaches to the study of cancer-stroma interactions
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1755-8794-3-14 http://hdl.handle.net/11449/21478 |
Resumo: | Background: The development and progression of cancer depend on its genetic characteristics as well as on the interactions with its microenvironment. Understanding these interactions may contribute to diagnostic and prognostic evaluations and to the development of new cancer therapies. Aiming to investigate potential mechanisms by which the tumor microenvironment might contribute to a cancer phenotype, we evaluated soluble paracrine factors produced by stromal and neoplastic cells which may influence proliferation and gene and protein expression.Methods: The study was carried out on the epithelial cancer cell line (Hep-2) and fibroblasts isolated from a primary oral cancer. We combined a conditioned-medium technique with subtraction hybridization approach, quantitative PCR and proteomics, in order to evaluate gene and protein expression influenced by soluble paracrine factors produced by stromal and neoplastic cells.Results: We observed that conditioned medium from fibroblast cultures (FCM) inhibited proliferation and induced apoptosis in Hep-2 cells. In neoplastic cells, 41 genes and 5 proteins exhibited changes in expression levels in response to FCM and, in fibroblasts, 17 genes and 2 proteins showed down-regulation in response to conditioned medium from Hep-2 cells (HCM). Nine genes were selected and the expression results of 6 down-regulated genes (ARID4A, CALR, GNB2L1, RNF10, SQSTM1, USP9X) were validated by real time PCR.Conclusions: A significant and common denominator in the results was the potential induction of signaling changes associated with immune or inflammatory response in the absence of a specific protein. |
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Genomics and proteomics approaches to the study of cancer-stroma interactionsBackground: The development and progression of cancer depend on its genetic characteristics as well as on the interactions with its microenvironment. Understanding these interactions may contribute to diagnostic and prognostic evaluations and to the development of new cancer therapies. Aiming to investigate potential mechanisms by which the tumor microenvironment might contribute to a cancer phenotype, we evaluated soluble paracrine factors produced by stromal and neoplastic cells which may influence proliferation and gene and protein expression.Methods: The study was carried out on the epithelial cancer cell line (Hep-2) and fibroblasts isolated from a primary oral cancer. We combined a conditioned-medium technique with subtraction hybridization approach, quantitative PCR and proteomics, in order to evaluate gene and protein expression influenced by soluble paracrine factors produced by stromal and neoplastic cells.Results: We observed that conditioned medium from fibroblast cultures (FCM) inhibited proliferation and induced apoptosis in Hep-2 cells. In neoplastic cells, 41 genes and 5 proteins exhibited changes in expression levels in response to FCM and, in fibroblasts, 17 genes and 2 proteins showed down-regulation in response to conditioned medium from Hep-2 cells (HCM). Nine genes were selected and the expression results of 6 down-regulated genes (ARID4A, CALR, GNB2L1, RNF10, SQSTM1, USP9X) were validated by real time PCR.Conclusions: A significant and common denominator in the results was the potential induction of signaling changes associated with immune or inflammatory response in the absence of a specific protein.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Rede Proteoma do Estado de São PauloLudwig Institute for Cancer ResearchConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sch Med FAMERP, Dept Mol Biol, Sao Jose do Rio Preto, BrazilSão Paulo State Univ UNESP, IBILCE, Dept Biol, Sao Jose do Rio Preto, BrazilSch Med FAMERP, Dept Otorhinolaryngol & Head & Neck Surg, Sao Jose do Rio Preto, BrazilUniv São Paulo, Inst Biociencias, Dept Genet & Evolutionary Biol, São Paulo, BrazilArnaldo Vieira de Carvalho Hosp, Dept Head & Neck Surg, São Paulo, BrazilUniv São Paulo, Sch Med, Dept Surg, Div Head & Neck Surg, BR-09500900 São Paulo, BrazilHeliopolis Hosp, Dept Head & Neck Surg, São Paulo, BrazilUNESP, Fac Engn Ilha Solteria, Dept Biol & Zootechny, Ilha Solteira, BrazilSão Paulo State Univ UNESP, IBILCE, Dept Biol, Sao Jose do Rio Preto, BrazilUNESP, Fac Engn Ilha Solteria, Dept Biol & Zootechny, Ilha Solteira, BrazilFAPESP: 04/12054-9FAPESP: 06/60162-0FAPESP: 04/14846-0FINEP: 01.07.0290.00Biomed Central Ltd.Faculdade de Medicina de São José do Rio Preto (FAMERP)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Arnaldo Vieira de Carvalho HospHeliopolis HospRodrigues-Lisoni, Flavia C. [UNESP]Peitl, Paulo [UNESP]Vidotto, AlessandraPolachini, Giovana M.Maniglia, Jose V.Carmona-Raphe, JulianaCunha, Bianca R.Henrique, TiagoSouza, Caique F.Teixeira, Rodrigo A. P. [UNESP]Fukuyama, Erica E.Michaluart, Pedrode Carvalho, Marcos B.Oliani, Sonia M. [UNESP]Tajara, Eloiza H.2014-05-20T14:00:48Z2014-05-20T14:00:48Z2010-05-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://dx.doi.org/10.1186/1755-8794-3-14Bmc Medical Genomics. London: Biomed Central Ltd., v. 3, p. 15, 2010.1755-8794http://hdl.handle.net/11449/2147810.1186/1755-8794-3-14WOS:000278383600001WOS000278383600001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBmc Medical Genomics3.3171,688info:eu-repo/semantics/openAccess2023-11-04T06:14:16Zoai:repositorio.unesp.br:11449/21478Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-04T06:14:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genomics and proteomics approaches to the study of cancer-stroma interactions |
title |
Genomics and proteomics approaches to the study of cancer-stroma interactions |
spellingShingle |
Genomics and proteomics approaches to the study of cancer-stroma interactions Rodrigues-Lisoni, Flavia C. [UNESP] |
title_short |
Genomics and proteomics approaches to the study of cancer-stroma interactions |
title_full |
Genomics and proteomics approaches to the study of cancer-stroma interactions |
title_fullStr |
Genomics and proteomics approaches to the study of cancer-stroma interactions |
title_full_unstemmed |
Genomics and proteomics approaches to the study of cancer-stroma interactions |
title_sort |
Genomics and proteomics approaches to the study of cancer-stroma interactions |
author |
Rodrigues-Lisoni, Flavia C. [UNESP] |
author_facet |
Rodrigues-Lisoni, Flavia C. [UNESP] Peitl, Paulo [UNESP] Vidotto, Alessandra Polachini, Giovana M. Maniglia, Jose V. Carmona-Raphe, Juliana Cunha, Bianca R. Henrique, Tiago Souza, Caique F. Teixeira, Rodrigo A. P. [UNESP] Fukuyama, Erica E. Michaluart, Pedro de Carvalho, Marcos B. Oliani, Sonia M. [UNESP] Tajara, Eloiza H. |
author_role |
author |
author2 |
Peitl, Paulo [UNESP] Vidotto, Alessandra Polachini, Giovana M. Maniglia, Jose V. Carmona-Raphe, Juliana Cunha, Bianca R. Henrique, Tiago Souza, Caique F. Teixeira, Rodrigo A. P. [UNESP] Fukuyama, Erica E. Michaluart, Pedro de Carvalho, Marcos B. Oliani, Sonia M. [UNESP] Tajara, Eloiza H. |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Faculdade de Medicina de São José do Rio Preto (FAMERP) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Arnaldo Vieira de Carvalho Hosp Heliopolis Hosp |
dc.contributor.author.fl_str_mv |
Rodrigues-Lisoni, Flavia C. [UNESP] Peitl, Paulo [UNESP] Vidotto, Alessandra Polachini, Giovana M. Maniglia, Jose V. Carmona-Raphe, Juliana Cunha, Bianca R. Henrique, Tiago Souza, Caique F. Teixeira, Rodrigo A. P. [UNESP] Fukuyama, Erica E. Michaluart, Pedro de Carvalho, Marcos B. Oliani, Sonia M. [UNESP] Tajara, Eloiza H. |
description |
Background: The development and progression of cancer depend on its genetic characteristics as well as on the interactions with its microenvironment. Understanding these interactions may contribute to diagnostic and prognostic evaluations and to the development of new cancer therapies. Aiming to investigate potential mechanisms by which the tumor microenvironment might contribute to a cancer phenotype, we evaluated soluble paracrine factors produced by stromal and neoplastic cells which may influence proliferation and gene and protein expression.Methods: The study was carried out on the epithelial cancer cell line (Hep-2) and fibroblasts isolated from a primary oral cancer. We combined a conditioned-medium technique with subtraction hybridization approach, quantitative PCR and proteomics, in order to evaluate gene and protein expression influenced by soluble paracrine factors produced by stromal and neoplastic cells.Results: We observed that conditioned medium from fibroblast cultures (FCM) inhibited proliferation and induced apoptosis in Hep-2 cells. In neoplastic cells, 41 genes and 5 proteins exhibited changes in expression levels in response to FCM and, in fibroblasts, 17 genes and 2 proteins showed down-regulation in response to conditioned medium from Hep-2 cells (HCM). Nine genes were selected and the expression results of 6 down-regulated genes (ARID4A, CALR, GNB2L1, RNF10, SQSTM1, USP9X) were validated by real time PCR.Conclusions: A significant and common denominator in the results was the potential induction of signaling changes associated with immune or inflammatory response in the absence of a specific protein. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-05-04 2014-05-20T14:00:48Z 2014-05-20T14:00:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1755-8794-3-14 Bmc Medical Genomics. London: Biomed Central Ltd., v. 3, p. 15, 2010. 1755-8794 http://hdl.handle.net/11449/21478 10.1186/1755-8794-3-14 WOS:000278383600001 WOS000278383600001.pdf |
url |
http://dx.doi.org/10.1186/1755-8794-3-14 http://hdl.handle.net/11449/21478 |
identifier_str_mv |
Bmc Medical Genomics. London: Biomed Central Ltd., v. 3, p. 15, 2010. 1755-8794 10.1186/1755-8794-3-14 WOS:000278383600001 WOS000278383600001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Medical Genomics 3.317 1,688 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
15 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964821842558976 |