Median preoptic nucleus mediates the cardiovascular recovery induced by hypertonic saline in hemorrhagic shock
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.hindawi.com/journals/tswj/2014/496121/ http://hdl.handle.net/11449/125715 |
Resumo: | Changes in plasma osmolarity, through central and peripheral osmoreceptors, activate the median preoptic nucleus (MnPO) that modulates autonomic and neuroendocrine adjustments. The present study sought to determine the participation of MnPO in the cardiovascular recovery induced by hypertonic saline infusion (HSI) in rats submitted to hemorrhagic shock. The recordings of mean arterial pressure (MAP) and renal vascular conductance (RVC) were carried out on male Wistar rats (250–300 g). Hemorrhagic shock was induced by blood withdrawal over 20 min until the MAP values of approximately 60 mmHg were attained. The nanoinjection (100 nL) of GABAA agonist (Muscimol 4 mM; experimental group (EXP)) or isotonic saline (NaCl 150 mM; control (CONT)) into MnPO was performed 2 min prior to intravenous overload of sodium through HSI (3 M NaCl, 1.8 mL/kg, b.wt.). Hemorrhagic shock reduced the MAP in control (62 ± 1.1 mmHg) and EXP (61 ± 0.4 mmHg) equipotently. The inhibition of MnPO impaired MAP (CONT: 104 ± 4.2 versus EXP: 60 ± 6.2 mmHg) and RVC (CONT: 6.4 ± 11.4 versus EXP: −53.5 ± 10.0) recovery 10 min after HSI. The overall results in this study demonstrated, for the first time, that the MnPO plays an essential role in the HSI induced resuscitation during hypovolemic hemorrhagic shock. |
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spelling |
Median preoptic nucleus mediates the cardiovascular recovery induced by hypertonic saline in hemorrhagic shockChanges in plasma osmolarity, through central and peripheral osmoreceptors, activate the median preoptic nucleus (MnPO) that modulates autonomic and neuroendocrine adjustments. The present study sought to determine the participation of MnPO in the cardiovascular recovery induced by hypertonic saline infusion (HSI) in rats submitted to hemorrhagic shock. The recordings of mean arterial pressure (MAP) and renal vascular conductance (RVC) were carried out on male Wistar rats (250–300 g). Hemorrhagic shock was induced by blood withdrawal over 20 min until the MAP values of approximately 60 mmHg were attained. The nanoinjection (100 nL) of GABAA agonist (Muscimol 4 mM; experimental group (EXP)) or isotonic saline (NaCl 150 mM; control (CONT)) into MnPO was performed 2 min prior to intravenous overload of sodium through HSI (3 M NaCl, 1.8 mL/kg, b.wt.). Hemorrhagic shock reduced the MAP in control (62 ± 1.1 mmHg) and EXP (61 ± 0.4 mmHg) equipotently. The inhibition of MnPO impaired MAP (CONT: 104 ± 4.2 versus EXP: 60 ± 6.2 mmHg) and RVC (CONT: 6.4 ± 11.4 versus EXP: −53.5 ± 10.0) recovery 10 min after HSI. The overall results in this study demonstrated, for the first time, that the MnPO plays an essential role in the HSI induced resuscitation during hypovolemic hemorrhagic shock.Fundação de Amparo à Pesquisa do Estado de Goiás (FAPEG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de Goiás, Centro de Neurociência e Fisiologia CardivascularUniversidade Federal de Goiás, Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências BiológicasUniversidade Federal de Goiás, Departamento de Ciências FisiológiasUniversidade Federal de Uberlândia, Faculdade de Educação FísicaUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Fisiologia e Patologia, Faculdade de Odontologia de AraraquaraFAPEG: 2012/0055431086FAPEG: 2009/10267000352CNPq: 477832/2010-5CNPq: 483411/2012-4Universidade Federal de Goiás (UFG)Universidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)Amaral, Nathalia OdaNaves, Lara MarquesFerreira-Neto, Marcos LuizFreiria-Oliveira, André HenriqueColombari, Eduardo [UNESP]Rosa, Daniel AlvesReis, Angela Adamski da SilvaIanzer, DanielliXavier, Carlos HenriquePedrino, Gustavo Rodrigues2015-08-06T16:12:54Z2015-08-06T16:12:54Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-9application/pdfhttp://www.hindawi.com/journals/tswj/2014/496121/The Scientific World Journal, v. 2014, p. 1-9, 2014.2356-6140http://hdl.handle.net/11449/12571510.1155/2014/496121ISSN2356-6140-2014-2014-01-09.pdf4544450092427426Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengThe Scientific World Journalinfo:eu-repo/semantics/openAccess2023-10-17T06:07:37Zoai:repositorio.unesp.br:11449/125715Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-17T06:07:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Median preoptic nucleus mediates the cardiovascular recovery induced by hypertonic saline in hemorrhagic shock |
title |
Median preoptic nucleus mediates the cardiovascular recovery induced by hypertonic saline in hemorrhagic shock |
spellingShingle |
Median preoptic nucleus mediates the cardiovascular recovery induced by hypertonic saline in hemorrhagic shock Amaral, Nathalia Oda |
title_short |
Median preoptic nucleus mediates the cardiovascular recovery induced by hypertonic saline in hemorrhagic shock |
title_full |
Median preoptic nucleus mediates the cardiovascular recovery induced by hypertonic saline in hemorrhagic shock |
title_fullStr |
Median preoptic nucleus mediates the cardiovascular recovery induced by hypertonic saline in hemorrhagic shock |
title_full_unstemmed |
Median preoptic nucleus mediates the cardiovascular recovery induced by hypertonic saline in hemorrhagic shock |
title_sort |
Median preoptic nucleus mediates the cardiovascular recovery induced by hypertonic saline in hemorrhagic shock |
author |
Amaral, Nathalia Oda |
author_facet |
Amaral, Nathalia Oda Naves, Lara Marques Ferreira-Neto, Marcos Luiz Freiria-Oliveira, André Henrique Colombari, Eduardo [UNESP] Rosa, Daniel Alves Reis, Angela Adamski da Silva Ianzer, Danielli Xavier, Carlos Henrique Pedrino, Gustavo Rodrigues |
author_role |
author |
author2 |
Naves, Lara Marques Ferreira-Neto, Marcos Luiz Freiria-Oliveira, André Henrique Colombari, Eduardo [UNESP] Rosa, Daniel Alves Reis, Angela Adamski da Silva Ianzer, Danielli Xavier, Carlos Henrique Pedrino, Gustavo Rodrigues |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Goiás (UFG) Universidade Federal de Uberlândia (UFU) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Amaral, Nathalia Oda Naves, Lara Marques Ferreira-Neto, Marcos Luiz Freiria-Oliveira, André Henrique Colombari, Eduardo [UNESP] Rosa, Daniel Alves Reis, Angela Adamski da Silva Ianzer, Danielli Xavier, Carlos Henrique Pedrino, Gustavo Rodrigues |
description |
Changes in plasma osmolarity, through central and peripheral osmoreceptors, activate the median preoptic nucleus (MnPO) that modulates autonomic and neuroendocrine adjustments. The present study sought to determine the participation of MnPO in the cardiovascular recovery induced by hypertonic saline infusion (HSI) in rats submitted to hemorrhagic shock. The recordings of mean arterial pressure (MAP) and renal vascular conductance (RVC) were carried out on male Wistar rats (250–300 g). Hemorrhagic shock was induced by blood withdrawal over 20 min until the MAP values of approximately 60 mmHg were attained. The nanoinjection (100 nL) of GABAA agonist (Muscimol 4 mM; experimental group (EXP)) or isotonic saline (NaCl 150 mM; control (CONT)) into MnPO was performed 2 min prior to intravenous overload of sodium through HSI (3 M NaCl, 1.8 mL/kg, b.wt.). Hemorrhagic shock reduced the MAP in control (62 ± 1.1 mmHg) and EXP (61 ± 0.4 mmHg) equipotently. The inhibition of MnPO impaired MAP (CONT: 104 ± 4.2 versus EXP: 60 ± 6.2 mmHg) and RVC (CONT: 6.4 ± 11.4 versus EXP: −53.5 ± 10.0) recovery 10 min after HSI. The overall results in this study demonstrated, for the first time, that the MnPO plays an essential role in the HSI induced resuscitation during hypovolemic hemorrhagic shock. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-08-06T16:12:54Z 2015-08-06T16:12:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.hindawi.com/journals/tswj/2014/496121/ The Scientific World Journal, v. 2014, p. 1-9, 2014. 2356-6140 http://hdl.handle.net/11449/125715 10.1155/2014/496121 ISSN2356-6140-2014-2014-01-09.pdf 4544450092427426 |
url |
http://www.hindawi.com/journals/tswj/2014/496121/ http://hdl.handle.net/11449/125715 |
identifier_str_mv |
The Scientific World Journal, v. 2014, p. 1-9, 2014. 2356-6140 10.1155/2014/496121 ISSN2356-6140-2014-2014-01-09.pdf 4544450092427426 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
The Scientific World Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-9 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964597066661888 |