Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats

Detalhes bibliográficos
Autor(a) principal: Naves, Lara Marques
Data de Publicação: 2018
Outros Autores: Marques, Stefanne Madalena, Mourao, Aline Andrade, Fajemiroye, James Oluwagbamigbe, Xavier, Carlos Henrique, Castro, Carlos Henrique de, Silva Rebelo, Ana Cristina, Rosa, Daniel Alves, Gomes, Rodrigo Mello, Colombari, Eduardo [UNESP], Pedrino, Gustavo Rodrigues
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1038/s41598-018-29310-z
http://hdl.handle.net/11449/164462
Resumo: The infusion of hypertonic saline solution (HSS) is known to be beneficial to the treatment of hypovolemic hemorrhage (HH). The central mechanism of HSS-induced cardiovascular and autonomic recovery of animals subjected to HH remains unclear. Hence, the present study evaluated the involvement of median preoptic nucleus (MnPO) and medullary noradrenergic neurons (A1 and A2) in HSS-induced cardiovascular and sympathetic responses in hemorrhagic rats. The wistar rats were subjected to specific lesion of noradrenergic neurons through the nanoinjections of anti-D beta H-saporin into caudal ventrolateral medulla (A1 neurons) and nucleus of the solitary tract (A2 neurons). After recovery, mean arterial pressure (MAP) and renal sympathetic nervous activity were recorded. The HH was performed through blood withdrawal until a MAP of 60 mmHg was attained. In sham rats, HSS infusion (3M NaCl) reestablished MAP without change in HH-induced sympathoinhibition. The muscimol (agonist of GABA(A) receptor) was nanoinjected in MnPO during HH and MnPO inhibition abolished the recovery of MAP and HSS-induced sympathoinhibition. Simultaneous lesions of A1 and A2 abolished MAP restoration and sympathoinhibition after HSS infusion. These results suggest that the recovery of MAP and HSS-induced sympathoinhibition in hemorrhaged rats depend on intact neural projections from A1 and A2 to MnPO.
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spelling Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic ratsThe infusion of hypertonic saline solution (HSS) is known to be beneficial to the treatment of hypovolemic hemorrhage (HH). The central mechanism of HSS-induced cardiovascular and autonomic recovery of animals subjected to HH remains unclear. Hence, the present study evaluated the involvement of median preoptic nucleus (MnPO) and medullary noradrenergic neurons (A1 and A2) in HSS-induced cardiovascular and sympathetic responses in hemorrhagic rats. The wistar rats were subjected to specific lesion of noradrenergic neurons through the nanoinjections of anti-D beta H-saporin into caudal ventrolateral medulla (A1 neurons) and nucleus of the solitary tract (A2 neurons). After recovery, mean arterial pressure (MAP) and renal sympathetic nervous activity were recorded. The HH was performed through blood withdrawal until a MAP of 60 mmHg was attained. In sham rats, HSS infusion (3M NaCl) reestablished MAP without change in HH-induced sympathoinhibition. The muscimol (agonist of GABA(A) receptor) was nanoinjected in MnPO during HH and MnPO inhibition abolished the recovery of MAP and HSS-induced sympathoinhibition. Simultaneous lesions of A1 and A2 abolished MAP restoration and sympathoinhibition after HSS infusion. These results suggest that the recovery of MAP and HSS-induced sympathoinhibition in hemorrhaged rats depend on intact neural projections from A1 and A2 to MnPO.Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Goias, Inst Ciencias Biol, Dept Ciencias Fisiol, Estr Campus S-N, BR-74690900 Goiania, Go, BrazilUniv Fed Goias, Fac Farm, Goiania, Go, BrazilUniv Fed Goias, Inst Ciencias Biol, Dept Morfol, Estr Campus S-N, BR-74690900 Goiania, Go, BrazilUniv Estadual Paulista, Dept Fisiol & Patol, Fac Odontol, Araraquara, SP, BrazilUniv Estadual Paulista, Dept Fisiol & Patol, Fac Odontol, Araraquara, SP, BrazilFundacao de Amparo a Pesquisa do Estado de Goias (FAPEG): 2012/0055431086Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG): 2009/10267000352CNPq: 477832/2010-5CNPq: 483411/2012-4CNPq: 447496/2014-0Nature Publishing GroupUniversidade Federal de Goiás (UFG)Universidade Estadual Paulista (Unesp)Naves, Lara MarquesMarques, Stefanne MadalenaMourao, Aline AndradeFajemiroye, James OluwagbamigbeXavier, Carlos HenriqueCastro, Carlos Henrique deSilva Rebelo, Ana CristinaRosa, Daniel AlvesGomes, Rodrigo MelloColombari, Eduardo [UNESP]Pedrino, Gustavo Rodrigues2018-11-26T17:54:39Z2018-11-26T17:54:39Z2018-07-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttp://dx.doi.org/10.1038/s41598-018-29310-zScientific Reports. London: Nature Publishing Group, v. 8, 14 p., 2018.2045-2322http://hdl.handle.net/11449/16446210.1038/s41598-018-29310-zWOS:000439806700002WOS000439806700002.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reports1,533info:eu-repo/semantics/openAccess2023-10-14T06:05:12Zoai:repositorio.unesp.br:11449/164462Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-14T06:05:12Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats
title Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats
spellingShingle Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats
Naves, Lara Marques
title_short Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats
title_full Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats
title_fullStr Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats
title_full_unstemmed Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats
title_sort Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats
author Naves, Lara Marques
author_facet Naves, Lara Marques
Marques, Stefanne Madalena
Mourao, Aline Andrade
Fajemiroye, James Oluwagbamigbe
Xavier, Carlos Henrique
Castro, Carlos Henrique de
Silva Rebelo, Ana Cristina
Rosa, Daniel Alves
Gomes, Rodrigo Mello
Colombari, Eduardo [UNESP]
Pedrino, Gustavo Rodrigues
author_role author
author2 Marques, Stefanne Madalena
Mourao, Aline Andrade
Fajemiroye, James Oluwagbamigbe
Xavier, Carlos Henrique
Castro, Carlos Henrique de
Silva Rebelo, Ana Cristina
Rosa, Daniel Alves
Gomes, Rodrigo Mello
Colombari, Eduardo [UNESP]
Pedrino, Gustavo Rodrigues
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Goiás (UFG)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Naves, Lara Marques
Marques, Stefanne Madalena
Mourao, Aline Andrade
Fajemiroye, James Oluwagbamigbe
Xavier, Carlos Henrique
Castro, Carlos Henrique de
Silva Rebelo, Ana Cristina
Rosa, Daniel Alves
Gomes, Rodrigo Mello
Colombari, Eduardo [UNESP]
Pedrino, Gustavo Rodrigues
description The infusion of hypertonic saline solution (HSS) is known to be beneficial to the treatment of hypovolemic hemorrhage (HH). The central mechanism of HSS-induced cardiovascular and autonomic recovery of animals subjected to HH remains unclear. Hence, the present study evaluated the involvement of median preoptic nucleus (MnPO) and medullary noradrenergic neurons (A1 and A2) in HSS-induced cardiovascular and sympathetic responses in hemorrhagic rats. The wistar rats were subjected to specific lesion of noradrenergic neurons through the nanoinjections of anti-D beta H-saporin into caudal ventrolateral medulla (A1 neurons) and nucleus of the solitary tract (A2 neurons). After recovery, mean arterial pressure (MAP) and renal sympathetic nervous activity were recorded. The HH was performed through blood withdrawal until a MAP of 60 mmHg was attained. In sham rats, HSS infusion (3M NaCl) reestablished MAP without change in HH-induced sympathoinhibition. The muscimol (agonist of GABA(A) receptor) was nanoinjected in MnPO during HH and MnPO inhibition abolished the recovery of MAP and HSS-induced sympathoinhibition. Simultaneous lesions of A1 and A2 abolished MAP restoration and sympathoinhibition after HSS infusion. These results suggest that the recovery of MAP and HSS-induced sympathoinhibition in hemorrhaged rats depend on intact neural projections from A1 and A2 to MnPO.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-26T17:54:39Z
2018-11-26T17:54:39Z
2018-07-26
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1038/s41598-018-29310-z
Scientific Reports. London: Nature Publishing Group, v. 8, 14 p., 2018.
2045-2322
http://hdl.handle.net/11449/164462
10.1038/s41598-018-29310-z
WOS:000439806700002
WOS000439806700002.pdf
url http://dx.doi.org/10.1038/s41598-018-29310-z
http://hdl.handle.net/11449/164462
identifier_str_mv Scientific Reports. London: Nature Publishing Group, v. 8, 14 p., 2018.
2045-2322
10.1038/s41598-018-29310-z
WOS:000439806700002
WOS000439806700002.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Scientific Reports
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dc.format.none.fl_str_mv 14
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
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instname_str Universidade Estadual Paulista (UNESP)
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institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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