Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-018-29310-z http://hdl.handle.net/11449/164462 |
Resumo: | The infusion of hypertonic saline solution (HSS) is known to be beneficial to the treatment of hypovolemic hemorrhage (HH). The central mechanism of HSS-induced cardiovascular and autonomic recovery of animals subjected to HH remains unclear. Hence, the present study evaluated the involvement of median preoptic nucleus (MnPO) and medullary noradrenergic neurons (A1 and A2) in HSS-induced cardiovascular and sympathetic responses in hemorrhagic rats. The wistar rats were subjected to specific lesion of noradrenergic neurons through the nanoinjections of anti-D beta H-saporin into caudal ventrolateral medulla (A1 neurons) and nucleus of the solitary tract (A2 neurons). After recovery, mean arterial pressure (MAP) and renal sympathetic nervous activity were recorded. The HH was performed through blood withdrawal until a MAP of 60 mmHg was attained. In sham rats, HSS infusion (3M NaCl) reestablished MAP without change in HH-induced sympathoinhibition. The muscimol (agonist of GABA(A) receptor) was nanoinjected in MnPO during HH and MnPO inhibition abolished the recovery of MAP and HSS-induced sympathoinhibition. Simultaneous lesions of A1 and A2 abolished MAP restoration and sympathoinhibition after HSS infusion. These results suggest that the recovery of MAP and HSS-induced sympathoinhibition in hemorrhaged rats depend on intact neural projections from A1 and A2 to MnPO. |
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Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic ratsThe infusion of hypertonic saline solution (HSS) is known to be beneficial to the treatment of hypovolemic hemorrhage (HH). The central mechanism of HSS-induced cardiovascular and autonomic recovery of animals subjected to HH remains unclear. Hence, the present study evaluated the involvement of median preoptic nucleus (MnPO) and medullary noradrenergic neurons (A1 and A2) in HSS-induced cardiovascular and sympathetic responses in hemorrhagic rats. The wistar rats were subjected to specific lesion of noradrenergic neurons through the nanoinjections of anti-D beta H-saporin into caudal ventrolateral medulla (A1 neurons) and nucleus of the solitary tract (A2 neurons). After recovery, mean arterial pressure (MAP) and renal sympathetic nervous activity were recorded. The HH was performed through blood withdrawal until a MAP of 60 mmHg was attained. In sham rats, HSS infusion (3M NaCl) reestablished MAP without change in HH-induced sympathoinhibition. The muscimol (agonist of GABA(A) receptor) was nanoinjected in MnPO during HH and MnPO inhibition abolished the recovery of MAP and HSS-induced sympathoinhibition. Simultaneous lesions of A1 and A2 abolished MAP restoration and sympathoinhibition after HSS infusion. These results suggest that the recovery of MAP and HSS-induced sympathoinhibition in hemorrhaged rats depend on intact neural projections from A1 and A2 to MnPO.Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Goias, Inst Ciencias Biol, Dept Ciencias Fisiol, Estr Campus S-N, BR-74690900 Goiania, Go, BrazilUniv Fed Goias, Fac Farm, Goiania, Go, BrazilUniv Fed Goias, Inst Ciencias Biol, Dept Morfol, Estr Campus S-N, BR-74690900 Goiania, Go, BrazilUniv Estadual Paulista, Dept Fisiol & Patol, Fac Odontol, Araraquara, SP, BrazilUniv Estadual Paulista, Dept Fisiol & Patol, Fac Odontol, Araraquara, SP, BrazilFundacao de Amparo a Pesquisa do Estado de Goias (FAPEG): 2012/0055431086Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG): 2009/10267000352CNPq: 477832/2010-5CNPq: 483411/2012-4CNPq: 447496/2014-0Nature Publishing GroupUniversidade Federal de Goiás (UFG)Universidade Estadual Paulista (Unesp)Naves, Lara MarquesMarques, Stefanne MadalenaMourao, Aline AndradeFajemiroye, James OluwagbamigbeXavier, Carlos HenriqueCastro, Carlos Henrique deSilva Rebelo, Ana CristinaRosa, Daniel AlvesGomes, Rodrigo MelloColombari, Eduardo [UNESP]Pedrino, Gustavo Rodrigues2018-11-26T17:54:39Z2018-11-26T17:54:39Z2018-07-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttp://dx.doi.org/10.1038/s41598-018-29310-zScientific Reports. London: Nature Publishing Group, v. 8, 14 p., 2018.2045-2322http://hdl.handle.net/11449/16446210.1038/s41598-018-29310-zWOS:000439806700002WOS000439806700002.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reports1,533info:eu-repo/semantics/openAccess2023-10-14T06:05:12Zoai:repositorio.unesp.br:11449/164462Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-14T06:05:12Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats |
title |
Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats |
spellingShingle |
Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats Naves, Lara Marques |
title_short |
Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats |
title_full |
Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats |
title_fullStr |
Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats |
title_full_unstemmed |
Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats |
title_sort |
Involvement of median preoptic nucleus and medullary noradrenergic neurons in cardiovascular and sympathetic responses of hemorrhagic rats |
author |
Naves, Lara Marques |
author_facet |
Naves, Lara Marques Marques, Stefanne Madalena Mourao, Aline Andrade Fajemiroye, James Oluwagbamigbe Xavier, Carlos Henrique Castro, Carlos Henrique de Silva Rebelo, Ana Cristina Rosa, Daniel Alves Gomes, Rodrigo Mello Colombari, Eduardo [UNESP] Pedrino, Gustavo Rodrigues |
author_role |
author |
author2 |
Marques, Stefanne Madalena Mourao, Aline Andrade Fajemiroye, James Oluwagbamigbe Xavier, Carlos Henrique Castro, Carlos Henrique de Silva Rebelo, Ana Cristina Rosa, Daniel Alves Gomes, Rodrigo Mello Colombari, Eduardo [UNESP] Pedrino, Gustavo Rodrigues |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Goiás (UFG) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Naves, Lara Marques Marques, Stefanne Madalena Mourao, Aline Andrade Fajemiroye, James Oluwagbamigbe Xavier, Carlos Henrique Castro, Carlos Henrique de Silva Rebelo, Ana Cristina Rosa, Daniel Alves Gomes, Rodrigo Mello Colombari, Eduardo [UNESP] Pedrino, Gustavo Rodrigues |
description |
The infusion of hypertonic saline solution (HSS) is known to be beneficial to the treatment of hypovolemic hemorrhage (HH). The central mechanism of HSS-induced cardiovascular and autonomic recovery of animals subjected to HH remains unclear. Hence, the present study evaluated the involvement of median preoptic nucleus (MnPO) and medullary noradrenergic neurons (A1 and A2) in HSS-induced cardiovascular and sympathetic responses in hemorrhagic rats. The wistar rats were subjected to specific lesion of noradrenergic neurons through the nanoinjections of anti-D beta H-saporin into caudal ventrolateral medulla (A1 neurons) and nucleus of the solitary tract (A2 neurons). After recovery, mean arterial pressure (MAP) and renal sympathetic nervous activity were recorded. The HH was performed through blood withdrawal until a MAP of 60 mmHg was attained. In sham rats, HSS infusion (3M NaCl) reestablished MAP without change in HH-induced sympathoinhibition. The muscimol (agonist of GABA(A) receptor) was nanoinjected in MnPO during HH and MnPO inhibition abolished the recovery of MAP and HSS-induced sympathoinhibition. Simultaneous lesions of A1 and A2 abolished MAP restoration and sympathoinhibition after HSS infusion. These results suggest that the recovery of MAP and HSS-induced sympathoinhibition in hemorrhaged rats depend on intact neural projections from A1 and A2 to MnPO. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:54:39Z 2018-11-26T17:54:39Z 2018-07-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-018-29310-z Scientific Reports. London: Nature Publishing Group, v. 8, 14 p., 2018. 2045-2322 http://hdl.handle.net/11449/164462 10.1038/s41598-018-29310-z WOS:000439806700002 WOS000439806700002.pdf |
url |
http://dx.doi.org/10.1038/s41598-018-29310-z http://hdl.handle.net/11449/164462 |
identifier_str_mv |
Scientific Reports. London: Nature Publishing Group, v. 8, 14 p., 2018. 2045-2322 10.1038/s41598-018-29310-z WOS:000439806700002 WOS000439806700002.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports 1,533 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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