Bacillus subtilis - capacity for enzymatic degradation, resistance to trace elements, antagonisms and siderophore production
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.21475/ajcs.21.15.05.p3206 http://hdl.handle.net/11449/221831 |
Resumo: | The use of microorganisms in agriculture as biofertilizers and biocontrol agents, in addition to their use in biotechnological practices, has been explored increasingly frequently over the years. Some bacteria, including Bacillus subtilis, have many capabilities related to promoting plant growth. The present study attempted to evaluate eight B. subtilis strains regarding their capacity for enzymatic degradation, resistance to trace elements, antagonism against phytopathogenic fungi and siderophore production. The tests were performed in plate dishes and test tubes with six repetitions for each bacterial isolate. The results showed that all isolates were able to perform enzymatic degradation to phosphatase, amylase and cellulase. Regarding resistance to trace elements, for Cd, 0.5 mmol L-1 was sufficient to prevent the development of strains 248, 263 and 320; for Cu, isolate 263 obtained greater resistance; for Zn, isolate 320 was inhibited at 2.0 mmol L-1, for Cr(III), isolates 290 and 291 showed greater resistance to the metal, whereas for Cr(VI), isolates showed the same resistance pattern; and for Ni, isolates showed the same resistance behavior. In vitro antagonism occurred for all isolates; however, the antagonism occurred at different intensities, except for isolate 291. The production of siderophores was identified for only six isolates: 287, 320, 309, 274, 263 and 248. These results establish a foundation for further investigations to clarify the conditions and/or characteristics required by isolates for a more effective performance, observing metabolic routes and genetic mechanisms. |
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Bacillus subtilis - capacity for enzymatic degradation, resistance to trace elements, antagonisms and siderophore productionAntagonismBacillus subtilisBioremediationBiotechnologySiderophoresThe use of microorganisms in agriculture as biofertilizers and biocontrol agents, in addition to their use in biotechnological practices, has been explored increasingly frequently over the years. Some bacteria, including Bacillus subtilis, have many capabilities related to promoting plant growth. The present study attempted to evaluate eight B. subtilis strains regarding their capacity for enzymatic degradation, resistance to trace elements, antagonism against phytopathogenic fungi and siderophore production. The tests were performed in plate dishes and test tubes with six repetitions for each bacterial isolate. The results showed that all isolates were able to perform enzymatic degradation to phosphatase, amylase and cellulase. Regarding resistance to trace elements, for Cd, 0.5 mmol L-1 was sufficient to prevent the development of strains 248, 263 and 320; for Cu, isolate 263 obtained greater resistance; for Zn, isolate 320 was inhibited at 2.0 mmol L-1, for Cr(III), isolates 290 and 291 showed greater resistance to the metal, whereas for Cr(VI), isolates showed the same resistance pattern; and for Ni, isolates showed the same resistance behavior. In vitro antagonism occurred for all isolates; however, the antagonism occurred at different intensities, except for isolate 291. The production of siderophores was identified for only six isolates: 287, 320, 309, 274, 263 and 248. These results establish a foundation for further investigations to clarify the conditions and/or characteristics required by isolates for a more effective performance, observing metabolic routes and genetic mechanisms.Graduate Program in Agricultural Microbiology Faculty of Agrarian and Veterinary Sciences State University of São Paulo (UNESP)Graduate Program in Food Science and Technology of the Federal Rural University of Rio de Janeiro (PPGCTA-UFRRJ)Graduate Program in Agricultural Microbiology Faculty of Agrarian and Veterinary Sciences State University of São Paulo (UNESP)Universidade Estadual Paulista (UNESP)Graduate Program in Food Science and Technology of the Federal Rural University of Rio de Janeiro (PPGCTA-UFRRJ)Santos, Bianca De Melo Silveira Dos [UNESP]Silva, Maura Santos Dos Reis De Andrade [UNESP]Chávez, Davy William HidalgoRigobelo, Everlon Cid [UNESP]2022-04-28T19:40:52Z2022-04-28T19:40:52Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article787-795http://dx.doi.org/10.21475/ajcs.21.15.05.p3206Australian Journal of Crop Science, v. 15, n. 5, p. 787-795, 2021.1835-27071835-2693http://hdl.handle.net/11449/22183110.21475/ajcs.21.15.05.p32062-s2.0-85108561326Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAustralian Journal of Crop Scienceinfo:eu-repo/semantics/openAccess2022-04-28T19:40:52Zoai:repositorio.unesp.br:11449/221831Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:33:44.532538Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bacillus subtilis - capacity for enzymatic degradation, resistance to trace elements, antagonisms and siderophore production |
title |
Bacillus subtilis - capacity for enzymatic degradation, resistance to trace elements, antagonisms and siderophore production |
spellingShingle |
Bacillus subtilis - capacity for enzymatic degradation, resistance to trace elements, antagonisms and siderophore production Santos, Bianca De Melo Silveira Dos [UNESP] Antagonism Bacillus subtilis Bioremediation Biotechnology Siderophores |
title_short |
Bacillus subtilis - capacity for enzymatic degradation, resistance to trace elements, antagonisms and siderophore production |
title_full |
Bacillus subtilis - capacity for enzymatic degradation, resistance to trace elements, antagonisms and siderophore production |
title_fullStr |
Bacillus subtilis - capacity for enzymatic degradation, resistance to trace elements, antagonisms and siderophore production |
title_full_unstemmed |
Bacillus subtilis - capacity for enzymatic degradation, resistance to trace elements, antagonisms and siderophore production |
title_sort |
Bacillus subtilis - capacity for enzymatic degradation, resistance to trace elements, antagonisms and siderophore production |
author |
Santos, Bianca De Melo Silveira Dos [UNESP] |
author_facet |
Santos, Bianca De Melo Silveira Dos [UNESP] Silva, Maura Santos Dos Reis De Andrade [UNESP] Chávez, Davy William Hidalgo Rigobelo, Everlon Cid [UNESP] |
author_role |
author |
author2 |
Silva, Maura Santos Dos Reis De Andrade [UNESP] Chávez, Davy William Hidalgo Rigobelo, Everlon Cid [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Graduate Program in Food Science and Technology of the Federal Rural University of Rio de Janeiro (PPGCTA-UFRRJ) |
dc.contributor.author.fl_str_mv |
Santos, Bianca De Melo Silveira Dos [UNESP] Silva, Maura Santos Dos Reis De Andrade [UNESP] Chávez, Davy William Hidalgo Rigobelo, Everlon Cid [UNESP] |
dc.subject.por.fl_str_mv |
Antagonism Bacillus subtilis Bioremediation Biotechnology Siderophores |
topic |
Antagonism Bacillus subtilis Bioremediation Biotechnology Siderophores |
description |
The use of microorganisms in agriculture as biofertilizers and biocontrol agents, in addition to their use in biotechnological practices, has been explored increasingly frequently over the years. Some bacteria, including Bacillus subtilis, have many capabilities related to promoting plant growth. The present study attempted to evaluate eight B. subtilis strains regarding their capacity for enzymatic degradation, resistance to trace elements, antagonism against phytopathogenic fungi and siderophore production. The tests were performed in plate dishes and test tubes with six repetitions for each bacterial isolate. The results showed that all isolates were able to perform enzymatic degradation to phosphatase, amylase and cellulase. Regarding resistance to trace elements, for Cd, 0.5 mmol L-1 was sufficient to prevent the development of strains 248, 263 and 320; for Cu, isolate 263 obtained greater resistance; for Zn, isolate 320 was inhibited at 2.0 mmol L-1, for Cr(III), isolates 290 and 291 showed greater resistance to the metal, whereas for Cr(VI), isolates showed the same resistance pattern; and for Ni, isolates showed the same resistance behavior. In vitro antagonism occurred for all isolates; however, the antagonism occurred at different intensities, except for isolate 291. The production of siderophores was identified for only six isolates: 287, 320, 309, 274, 263 and 248. These results establish a foundation for further investigations to clarify the conditions and/or characteristics required by isolates for a more effective performance, observing metabolic routes and genetic mechanisms. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-28T19:40:52Z 2022-04-28T19:40:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.21475/ajcs.21.15.05.p3206 Australian Journal of Crop Science, v. 15, n. 5, p. 787-795, 2021. 1835-2707 1835-2693 http://hdl.handle.net/11449/221831 10.21475/ajcs.21.15.05.p3206 2-s2.0-85108561326 |
url |
http://dx.doi.org/10.21475/ajcs.21.15.05.p3206 http://hdl.handle.net/11449/221831 |
identifier_str_mv |
Australian Journal of Crop Science, v. 15, n. 5, p. 787-795, 2021. 1835-2707 1835-2693 10.21475/ajcs.21.15.05.p3206 2-s2.0-85108561326 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Australian Journal of Crop Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
787-795 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128248215764992 |