Leptospira sp. vertical transmission in ewes maintained in semiarid conditions
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anireprosci.2020.106530 http://hdl.handle.net/11449/200648 |
Resumo: | Leptospirosis is a globally distributed disease associated with reproductive failures in livestock; however, its pathogenesis has not been fully elucidated. Results from the present study indicate there is a presence of Leptospira sp. in organs and fluids of fetuses from ewes slaughtered in the semiarid region of Brazil. Twenty-nine fetuses from 23 ewes determined to be Leptospira sp.-positive using PCR were sampled (14 and 15 in dry and rainy seasons, respectively). Fetal samples of blood, central nervous system (CNS), lung, liver, spleen, stomach contents, peritoneal fluid, kidney, bladder, urine and reproductive system were collected. Diagnostic methods included the microscopic agglutination test (MAT), polymerase chain reaction (PCR) and bacterial isolation. Of the 29 fetuses, 24 (82.8 %) had at least one Leptospira sp.-positive organ or fluid, as determined using PCR, and of a total of 209 samples, 62 (29.7 %) contained leptospiral DNA. Of the 99 samples collected during the dry season, 42 (42.4 %) were positive, and of 110 samples collected during the rainy season, 20 (18.2 %) were positive (P = 0.0001). There was deoxyribonucleic acid (DNA) sequencing of three samples of kidney, CNS and liver, and in all of these, there was 99.3 % similarity with Leptospira interrogans. Leptospires were present in cultures of pooled samples from fetuses with deformities. Results indicate there is vertical (maternal-to-fetus) transmission which would represent an alternative transmission route for the spread of Leptospira sp. in ewes, suggesting molecular detection is essential in the investigation of leptospirosis in fetuses to identify animals that have been infected with this bacterium. |
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Leptospira sp. vertical transmission in ewes maintained in semiarid conditionsDirect transmissionExtrarenal leptospirosisFetusesPCRPlacentaLeptospirosis is a globally distributed disease associated with reproductive failures in livestock; however, its pathogenesis has not been fully elucidated. Results from the present study indicate there is a presence of Leptospira sp. in organs and fluids of fetuses from ewes slaughtered in the semiarid region of Brazil. Twenty-nine fetuses from 23 ewes determined to be Leptospira sp.-positive using PCR were sampled (14 and 15 in dry and rainy seasons, respectively). Fetal samples of blood, central nervous system (CNS), lung, liver, spleen, stomach contents, peritoneal fluid, kidney, bladder, urine and reproductive system were collected. Diagnostic methods included the microscopic agglutination test (MAT), polymerase chain reaction (PCR) and bacterial isolation. Of the 29 fetuses, 24 (82.8 %) had at least one Leptospira sp.-positive organ or fluid, as determined using PCR, and of a total of 209 samples, 62 (29.7 %) contained leptospiral DNA. Of the 99 samples collected during the dry season, 42 (42.4 %) were positive, and of 110 samples collected during the rainy season, 20 (18.2 %) were positive (P = 0.0001). There was deoxyribonucleic acid (DNA) sequencing of three samples of kidney, CNS and liver, and in all of these, there was 99.3 % similarity with Leptospira interrogans. Leptospires were present in cultures of pooled samples from fetuses with deformities. Results indicate there is vertical (maternal-to-fetus) transmission which would represent an alternative transmission route for the spread of Leptospira sp. in ewes, suggesting molecular detection is essential in the investigation of leptospirosis in fetuses to identify animals that have been infected with this bacterium.Transmissible Diseases Laboratory Center of Rural Health and Technology UFCG Federal University of Campina GrandeDepartment of Veterinary Sciences UFPB Federal University of ParaíbaDepartment of Microbiology and Immunology UNESP São Paulo State University Júlio de Mesquita FilhoDepartment of Microbiology and Immunology UNESP São Paulo State University Júlio de Mesquita FilhoFederal University of Campina GrandeUniversidade Federal da Paraíba (UFPB)Universidade Estadual Paulista (Unesp)Nogueira, Denise Batistada Costa, Flávia Teresa Ribeirode Sousa Bezerra, CamilaSoares, Rafael Rodriguesda Costa Barnabé, Nathanael NatércioFalcão, Brunna Muniz RodriguesSilva, Maria Luana Cristiny Rodriguesda Costa, Diego FigueiredoAraújo, João Pessoa [UNESP]Malossi, Camila Dantas [UNESP]Ullmann, Leila Sabrina [UNESP]Alves, Clebert Joséde Azevedo, Sérgio Santos2020-12-12T02:12:18Z2020-12-12T02:12:18Z2020-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.anireprosci.2020.106530Animal Reproduction Science, v. 219.0378-4320http://hdl.handle.net/11449/20064810.1016/j.anireprosci.2020.1065302-s2.0-85087008430Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Scienceinfo:eu-repo/semantics/openAccess2021-10-23T14:54:17Zoai:repositorio.unesp.br:11449/200648Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:49:24.582078Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Leptospira sp. vertical transmission in ewes maintained in semiarid conditions |
title |
Leptospira sp. vertical transmission in ewes maintained in semiarid conditions |
spellingShingle |
Leptospira sp. vertical transmission in ewes maintained in semiarid conditions Nogueira, Denise Batista Direct transmission Extrarenal leptospirosis Fetuses PCR Placenta |
title_short |
Leptospira sp. vertical transmission in ewes maintained in semiarid conditions |
title_full |
Leptospira sp. vertical transmission in ewes maintained in semiarid conditions |
title_fullStr |
Leptospira sp. vertical transmission in ewes maintained in semiarid conditions |
title_full_unstemmed |
Leptospira sp. vertical transmission in ewes maintained in semiarid conditions |
title_sort |
Leptospira sp. vertical transmission in ewes maintained in semiarid conditions |
author |
Nogueira, Denise Batista |
author_facet |
Nogueira, Denise Batista da Costa, Flávia Teresa Ribeiro de Sousa Bezerra, Camila Soares, Rafael Rodrigues da Costa Barnabé, Nathanael Natércio Falcão, Brunna Muniz Rodrigues Silva, Maria Luana Cristiny Rodrigues da Costa, Diego Figueiredo Araújo, João Pessoa [UNESP] Malossi, Camila Dantas [UNESP] Ullmann, Leila Sabrina [UNESP] Alves, Clebert José de Azevedo, Sérgio Santos |
author_role |
author |
author2 |
da Costa, Flávia Teresa Ribeiro de Sousa Bezerra, Camila Soares, Rafael Rodrigues da Costa Barnabé, Nathanael Natércio Falcão, Brunna Muniz Rodrigues Silva, Maria Luana Cristiny Rodrigues da Costa, Diego Figueiredo Araújo, João Pessoa [UNESP] Malossi, Camila Dantas [UNESP] Ullmann, Leila Sabrina [UNESP] Alves, Clebert José de Azevedo, Sérgio Santos |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Campina Grande Universidade Federal da Paraíba (UFPB) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Nogueira, Denise Batista da Costa, Flávia Teresa Ribeiro de Sousa Bezerra, Camila Soares, Rafael Rodrigues da Costa Barnabé, Nathanael Natércio Falcão, Brunna Muniz Rodrigues Silva, Maria Luana Cristiny Rodrigues da Costa, Diego Figueiredo Araújo, João Pessoa [UNESP] Malossi, Camila Dantas [UNESP] Ullmann, Leila Sabrina [UNESP] Alves, Clebert José de Azevedo, Sérgio Santos |
dc.subject.por.fl_str_mv |
Direct transmission Extrarenal leptospirosis Fetuses PCR Placenta |
topic |
Direct transmission Extrarenal leptospirosis Fetuses PCR Placenta |
description |
Leptospirosis is a globally distributed disease associated with reproductive failures in livestock; however, its pathogenesis has not been fully elucidated. Results from the present study indicate there is a presence of Leptospira sp. in organs and fluids of fetuses from ewes slaughtered in the semiarid region of Brazil. Twenty-nine fetuses from 23 ewes determined to be Leptospira sp.-positive using PCR were sampled (14 and 15 in dry and rainy seasons, respectively). Fetal samples of blood, central nervous system (CNS), lung, liver, spleen, stomach contents, peritoneal fluid, kidney, bladder, urine and reproductive system were collected. Diagnostic methods included the microscopic agglutination test (MAT), polymerase chain reaction (PCR) and bacterial isolation. Of the 29 fetuses, 24 (82.8 %) had at least one Leptospira sp.-positive organ or fluid, as determined using PCR, and of a total of 209 samples, 62 (29.7 %) contained leptospiral DNA. Of the 99 samples collected during the dry season, 42 (42.4 %) were positive, and of 110 samples collected during the rainy season, 20 (18.2 %) were positive (P = 0.0001). There was deoxyribonucleic acid (DNA) sequencing of three samples of kidney, CNS and liver, and in all of these, there was 99.3 % similarity with Leptospira interrogans. Leptospires were present in cultures of pooled samples from fetuses with deformities. Results indicate there is vertical (maternal-to-fetus) transmission which would represent an alternative transmission route for the spread of Leptospira sp. in ewes, suggesting molecular detection is essential in the investigation of leptospirosis in fetuses to identify animals that have been infected with this bacterium. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:12:18Z 2020-12-12T02:12:18Z 2020-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anireprosci.2020.106530 Animal Reproduction Science, v. 219. 0378-4320 http://hdl.handle.net/11449/200648 10.1016/j.anireprosci.2020.106530 2-s2.0-85087008430 |
url |
http://dx.doi.org/10.1016/j.anireprosci.2020.106530 http://hdl.handle.net/11449/200648 |
identifier_str_mv |
Animal Reproduction Science, v. 219. 0378-4320 10.1016/j.anireprosci.2020.106530 2-s2.0-85087008430 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Reproduction Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128984628592640 |