Leptospira sp. vertical transmission in ewes maintained in semiarid conditions

Detalhes bibliográficos
Autor(a) principal: Nogueira, Denise Batista
Data de Publicação: 2020
Outros Autores: da Costa, Flávia Teresa Ribeiro, de Sousa Bezerra, Camila, Soares, Rafael Rodrigues, da Costa Barnabé, Nathanael Natércio, Falcão, Brunna Muniz Rodrigues, Silva, Maria Luana Cristiny Rodrigues, da Costa, Diego Figueiredo, Araújo, João Pessoa [UNESP], Malossi, Camila Dantas [UNESP], Ullmann, Leila Sabrina [UNESP], Alves, Clebert José, de Azevedo, Sérgio Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.anireprosci.2020.106530
http://hdl.handle.net/11449/200648
Resumo: Leptospirosis is a globally distributed disease associated with reproductive failures in livestock; however, its pathogenesis has not been fully elucidated. Results from the present study indicate there is a presence of Leptospira sp. in organs and fluids of fetuses from ewes slaughtered in the semiarid region of Brazil. Twenty-nine fetuses from 23 ewes determined to be Leptospira sp.-positive using PCR were sampled (14 and 15 in dry and rainy seasons, respectively). Fetal samples of blood, central nervous system (CNS), lung, liver, spleen, stomach contents, peritoneal fluid, kidney, bladder, urine and reproductive system were collected. Diagnostic methods included the microscopic agglutination test (MAT), polymerase chain reaction (PCR) and bacterial isolation. Of the 29 fetuses, 24 (82.8 %) had at least one Leptospira sp.-positive organ or fluid, as determined using PCR, and of a total of 209 samples, 62 (29.7 %) contained leptospiral DNA. Of the 99 samples collected during the dry season, 42 (42.4 %) were positive, and of 110 samples collected during the rainy season, 20 (18.2 %) were positive (P = 0.0001). There was deoxyribonucleic acid (DNA) sequencing of three samples of kidney, CNS and liver, and in all of these, there was 99.3 % similarity with Leptospira interrogans. Leptospires were present in cultures of pooled samples from fetuses with deformities. Results indicate there is vertical (maternal-to-fetus) transmission which would represent an alternative transmission route for the spread of Leptospira sp. in ewes, suggesting molecular detection is essential in the investigation of leptospirosis in fetuses to identify animals that have been infected with this bacterium.
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spelling Leptospira sp. vertical transmission in ewes maintained in semiarid conditionsDirect transmissionExtrarenal leptospirosisFetusesPCRPlacentaLeptospirosis is a globally distributed disease associated with reproductive failures in livestock; however, its pathogenesis has not been fully elucidated. Results from the present study indicate there is a presence of Leptospira sp. in organs and fluids of fetuses from ewes slaughtered in the semiarid region of Brazil. Twenty-nine fetuses from 23 ewes determined to be Leptospira sp.-positive using PCR were sampled (14 and 15 in dry and rainy seasons, respectively). Fetal samples of blood, central nervous system (CNS), lung, liver, spleen, stomach contents, peritoneal fluid, kidney, bladder, urine and reproductive system were collected. Diagnostic methods included the microscopic agglutination test (MAT), polymerase chain reaction (PCR) and bacterial isolation. Of the 29 fetuses, 24 (82.8 %) had at least one Leptospira sp.-positive organ or fluid, as determined using PCR, and of a total of 209 samples, 62 (29.7 %) contained leptospiral DNA. Of the 99 samples collected during the dry season, 42 (42.4 %) were positive, and of 110 samples collected during the rainy season, 20 (18.2 %) were positive (P = 0.0001). There was deoxyribonucleic acid (DNA) sequencing of three samples of kidney, CNS and liver, and in all of these, there was 99.3 % similarity with Leptospira interrogans. Leptospires were present in cultures of pooled samples from fetuses with deformities. Results indicate there is vertical (maternal-to-fetus) transmission which would represent an alternative transmission route for the spread of Leptospira sp. in ewes, suggesting molecular detection is essential in the investigation of leptospirosis in fetuses to identify animals that have been infected with this bacterium.Transmissible Diseases Laboratory Center of Rural Health and Technology UFCG Federal University of Campina GrandeDepartment of Veterinary Sciences UFPB Federal University of ParaíbaDepartment of Microbiology and Immunology UNESP São Paulo State University Júlio de Mesquita FilhoDepartment of Microbiology and Immunology UNESP São Paulo State University Júlio de Mesquita FilhoFederal University of Campina GrandeUniversidade Federal da Paraíba (UFPB)Universidade Estadual Paulista (Unesp)Nogueira, Denise Batistada Costa, Flávia Teresa Ribeirode Sousa Bezerra, CamilaSoares, Rafael Rodriguesda Costa Barnabé, Nathanael NatércioFalcão, Brunna Muniz RodriguesSilva, Maria Luana Cristiny Rodriguesda Costa, Diego FigueiredoAraújo, João Pessoa [UNESP]Malossi, Camila Dantas [UNESP]Ullmann, Leila Sabrina [UNESP]Alves, Clebert Joséde Azevedo, Sérgio Santos2020-12-12T02:12:18Z2020-12-12T02:12:18Z2020-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.anireprosci.2020.106530Animal Reproduction Science, v. 219.0378-4320http://hdl.handle.net/11449/20064810.1016/j.anireprosci.2020.1065302-s2.0-85087008430Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Scienceinfo:eu-repo/semantics/openAccess2021-10-23T14:54:17Zoai:repositorio.unesp.br:11449/200648Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:49:24.582078Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Leptospira sp. vertical transmission in ewes maintained in semiarid conditions
title Leptospira sp. vertical transmission in ewes maintained in semiarid conditions
spellingShingle Leptospira sp. vertical transmission in ewes maintained in semiarid conditions
Nogueira, Denise Batista
Direct transmission
Extrarenal leptospirosis
Fetuses
PCR
Placenta
title_short Leptospira sp. vertical transmission in ewes maintained in semiarid conditions
title_full Leptospira sp. vertical transmission in ewes maintained in semiarid conditions
title_fullStr Leptospira sp. vertical transmission in ewes maintained in semiarid conditions
title_full_unstemmed Leptospira sp. vertical transmission in ewes maintained in semiarid conditions
title_sort Leptospira sp. vertical transmission in ewes maintained in semiarid conditions
author Nogueira, Denise Batista
author_facet Nogueira, Denise Batista
da Costa, Flávia Teresa Ribeiro
de Sousa Bezerra, Camila
Soares, Rafael Rodrigues
da Costa Barnabé, Nathanael Natércio
Falcão, Brunna Muniz Rodrigues
Silva, Maria Luana Cristiny Rodrigues
da Costa, Diego Figueiredo
Araújo, João Pessoa [UNESP]
Malossi, Camila Dantas [UNESP]
Ullmann, Leila Sabrina [UNESP]
Alves, Clebert José
de Azevedo, Sérgio Santos
author_role author
author2 da Costa, Flávia Teresa Ribeiro
de Sousa Bezerra, Camila
Soares, Rafael Rodrigues
da Costa Barnabé, Nathanael Natércio
Falcão, Brunna Muniz Rodrigues
Silva, Maria Luana Cristiny Rodrigues
da Costa, Diego Figueiredo
Araújo, João Pessoa [UNESP]
Malossi, Camila Dantas [UNESP]
Ullmann, Leila Sabrina [UNESP]
Alves, Clebert José
de Azevedo, Sérgio Santos
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Federal University of Campina Grande
Universidade Federal da Paraíba (UFPB)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Nogueira, Denise Batista
da Costa, Flávia Teresa Ribeiro
de Sousa Bezerra, Camila
Soares, Rafael Rodrigues
da Costa Barnabé, Nathanael Natércio
Falcão, Brunna Muniz Rodrigues
Silva, Maria Luana Cristiny Rodrigues
da Costa, Diego Figueiredo
Araújo, João Pessoa [UNESP]
Malossi, Camila Dantas [UNESP]
Ullmann, Leila Sabrina [UNESP]
Alves, Clebert José
de Azevedo, Sérgio Santos
dc.subject.por.fl_str_mv Direct transmission
Extrarenal leptospirosis
Fetuses
PCR
Placenta
topic Direct transmission
Extrarenal leptospirosis
Fetuses
PCR
Placenta
description Leptospirosis is a globally distributed disease associated with reproductive failures in livestock; however, its pathogenesis has not been fully elucidated. Results from the present study indicate there is a presence of Leptospira sp. in organs and fluids of fetuses from ewes slaughtered in the semiarid region of Brazil. Twenty-nine fetuses from 23 ewes determined to be Leptospira sp.-positive using PCR were sampled (14 and 15 in dry and rainy seasons, respectively). Fetal samples of blood, central nervous system (CNS), lung, liver, spleen, stomach contents, peritoneal fluid, kidney, bladder, urine and reproductive system were collected. Diagnostic methods included the microscopic agglutination test (MAT), polymerase chain reaction (PCR) and bacterial isolation. Of the 29 fetuses, 24 (82.8 %) had at least one Leptospira sp.-positive organ or fluid, as determined using PCR, and of a total of 209 samples, 62 (29.7 %) contained leptospiral DNA. Of the 99 samples collected during the dry season, 42 (42.4 %) were positive, and of 110 samples collected during the rainy season, 20 (18.2 %) were positive (P = 0.0001). There was deoxyribonucleic acid (DNA) sequencing of three samples of kidney, CNS and liver, and in all of these, there was 99.3 % similarity with Leptospira interrogans. Leptospires were present in cultures of pooled samples from fetuses with deformities. Results indicate there is vertical (maternal-to-fetus) transmission which would represent an alternative transmission route for the spread of Leptospira sp. in ewes, suggesting molecular detection is essential in the investigation of leptospirosis in fetuses to identify animals that have been infected with this bacterium.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:12:18Z
2020-12-12T02:12:18Z
2020-08-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.anireprosci.2020.106530
Animal Reproduction Science, v. 219.
0378-4320
http://hdl.handle.net/11449/200648
10.1016/j.anireprosci.2020.106530
2-s2.0-85087008430
url http://dx.doi.org/10.1016/j.anireprosci.2020.106530
http://hdl.handle.net/11449/200648
identifier_str_mv Animal Reproduction Science, v. 219.
0378-4320
10.1016/j.anireprosci.2020.106530
2-s2.0-85087008430
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Animal Reproduction Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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