In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells

Detalhes bibliográficos
Autor(a) principal: Pereira-Junior, Oduvaldo Câmara Marques
Data de Publicação: 2013
Outros Autores: Rahal, Sheila Canevese [UNESP], Lima-Neto, João Ferreira, Landim-Alvarenga, Fernanda da Cruz [UNESP], Monteiro, Frederico Ozanan Barros
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502013000500006
http://hdl.handle.net/11449/29025
Resumo: PURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.
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spelling In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cellsHydroxyapatitesBone MatrixCastor OilPolyurethanesStem CellsPURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)UNESP School of Veterinary Medicine and Animal Science Department of Veterinary Surgery and AnesthesiologyUNESP School of Veterinary Medicine and Animal Department of Animal Reproduction and Veterinary RadiologyFederal Rural University of the Amazon Institute of Animal Health and ProductionUNESP School of Veterinary Medicine and Animal Science Department of Veterinary Surgery and AnesthesiologyUNESP School of Veterinary Medicine and Animal Department of Animal Reproduction and Veterinary RadiologySociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Estadual Paulista (Unesp)Federal Rural University of the Amazon Institute of Animal Health and ProductionPereira-Junior, Oduvaldo Câmara MarquesRahal, Sheila Canevese [UNESP]Lima-Neto, João FerreiraLandim-Alvarenga, Fernanda da Cruz [UNESP]Monteiro, Frederico Ozanan Barros2014-05-20T15:14:03Z2014-05-20T15:14:03Z2013-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article353-360application/pdfhttp://dx.doi.org/10.1590/S0102-86502013000500006Acta Cirúrgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 5, p. 353-360, 2013.0102-8650http://hdl.handle.net/11449/2902510.1590/S0102-86502013000500006S0102-86502013000500006WOS:000319994200006S0102-86502013000500006.pdf14974332653901940000-0002-9211-4093SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirúrgica Brasileira0.9330,395info:eu-repo/semantics/openAccess2023-10-20T06:05:59Zoai:repositorio.unesp.br:11449/29025Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:25:02.339412Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
title In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
spellingShingle In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
Pereira-Junior, Oduvaldo Câmara Marques
Hydroxyapatites
Bone Matrix
Castor Oil
Polyurethanes
Stem Cells
title_short In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
title_full In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
title_fullStr In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
title_full_unstemmed In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
title_sort In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
author Pereira-Junior, Oduvaldo Câmara Marques
author_facet Pereira-Junior, Oduvaldo Câmara Marques
Rahal, Sheila Canevese [UNESP]
Lima-Neto, João Ferreira
Landim-Alvarenga, Fernanda da Cruz [UNESP]
Monteiro, Frederico Ozanan Barros
author_role author
author2 Rahal, Sheila Canevese [UNESP]
Lima-Neto, João Ferreira
Landim-Alvarenga, Fernanda da Cruz [UNESP]
Monteiro, Frederico Ozanan Barros
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Federal Rural University of the Amazon Institute of Animal Health and Production
dc.contributor.author.fl_str_mv Pereira-Junior, Oduvaldo Câmara Marques
Rahal, Sheila Canevese [UNESP]
Lima-Neto, João Ferreira
Landim-Alvarenga, Fernanda da Cruz [UNESP]
Monteiro, Frederico Ozanan Barros
dc.subject.por.fl_str_mv Hydroxyapatites
Bone Matrix
Castor Oil
Polyurethanes
Stem Cells
topic Hydroxyapatites
Bone Matrix
Castor Oil
Polyurethanes
Stem Cells
description PURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.
publishDate 2013
dc.date.none.fl_str_mv 2013-05-01
2014-05-20T15:14:03Z
2014-05-20T15:14:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502013000500006
Acta Cirúrgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 5, p. 353-360, 2013.
0102-8650
http://hdl.handle.net/11449/29025
10.1590/S0102-86502013000500006
S0102-86502013000500006
WOS:000319994200006
S0102-86502013000500006.pdf
1497433265390194
0000-0002-9211-4093
url http://dx.doi.org/10.1590/S0102-86502013000500006
http://hdl.handle.net/11449/29025
identifier_str_mv Acta Cirúrgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 5, p. 353-360, 2013.
0102-8650
10.1590/S0102-86502013000500006
S0102-86502013000500006
WOS:000319994200006
S0102-86502013000500006.pdf
1497433265390194
0000-0002-9211-4093
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirúrgica Brasileira
0.933
0,395
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 353-360
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128511672582144

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