Origin of B chromosomes in the genus Astyanax (Characiformes, Characidae) and the limits of chromosome painting
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00438-016-1195-y http://hdl.handle.net/11449/161537 |
Resumo: | Eukaryote genomes are frequently burdened with the presence of supernumerary (B) chromosomes. Their origin is frequently investigated by chromosome painting, under the hypothesis that sharing the repetitive DNA sequences contained in the painting probes is a sign of common descent. However, the intragenomic mobility of many anonymous DNA sequences contained in these probes (e.g., transposable elements) adds high uncertainty to this conclusion. Here we test the validity of chromosome painting to investigate B chromosome origin by comparing its results for seven B chromosome types in two fish species genus Astyanax, with those obtained (1) by means of the physical mapping of 18S ribosomal DNA (rDNA), H1 histone genes, the As51 satellite DNA and the (AC)(15) microsatellite, and (2) by comparing the nucleotide sequence of one of these families (ITS regions from ribosomal DNA) between genomic DNA from B-lacking individuals in both species and the microdissected DNA from two metacentric B chromosomes found in these same species. Intra- and inter-specific painting suggested that all B chromosomes that were assayed shared homologous DNA sequences among them, as well as with a variable number of A chromosomes in each species. This finding would be consistent with a common origin for all seven B chromosomes analyzed. By contrast, the physical mapping of repetitive DNA sequences failed to give support to this hypothesis, as no more than two B-types shared a given repetitive DNA. Finally, sequence analysis of the ITS regions suggested that at least some of the B chromosomes could have had a common origin. |
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spelling |
Origin of B chromosomes in the genus Astyanax (Characiformes, Characidae) and the limits of chromosome paintingB chromosomerDNASatellite DNAMicrosatellitesChromosome paintingEukaryote genomes are frequently burdened with the presence of supernumerary (B) chromosomes. Their origin is frequently investigated by chromosome painting, under the hypothesis that sharing the repetitive DNA sequences contained in the painting probes is a sign of common descent. However, the intragenomic mobility of many anonymous DNA sequences contained in these probes (e.g., transposable elements) adds high uncertainty to this conclusion. Here we test the validity of chromosome painting to investigate B chromosome origin by comparing its results for seven B chromosome types in two fish species genus Astyanax, with those obtained (1) by means of the physical mapping of 18S ribosomal DNA (rDNA), H1 histone genes, the As51 satellite DNA and the (AC)(15) microsatellite, and (2) by comparing the nucleotide sequence of one of these families (ITS regions from ribosomal DNA) between genomic DNA from B-lacking individuals in both species and the microdissected DNA from two metacentric B chromosomes found in these same species. Intra- and inter-specific painting suggested that all B chromosomes that were assayed shared homologous DNA sequences among them, as well as with a variable number of A chromosomes in each species. This finding would be consistent with a common origin for all seven B chromosomes analyzed. By contrast, the physical mapping of repetitive DNA sequences failed to give support to this hypothesis, as no more than two B-types shared a given repetitive DNA. Finally, sequence analysis of the ITS regions suggested that at least some of the B chromosomes could have had a common origin.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Inst Biociencias, Dept Morfol, Distrito Rubiao Jr S-N, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, Campus Bauru, BR-17033360 Bauru, SP, BrazilUniv Granada, Dept Genet, E-18071 Granada, SpainUniv Estadual Paulista, CAUNESP, BR-14884900 Jaboticabal, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Distrito Rubiao Jr S-N, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, Campus Bauru, BR-17033360 Bauru, SP, BrazilUniv Estadual Paulista, CAUNESP, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2011/16825-3SpringerUniversidade Estadual Paulista (Unesp)Univ GranadaSilva, Duilio M. Z. de A. [UNESP]Daniel, Sandro Natal [UNESP]Camacho, Juan Pedro M.Utsunomia, Ricardo [UNESP]Ruiz-Ruano, Francisco J.Penitente, Manolo [UNESP]Pansonato-Alves, Jose Carlos [UNESP]Hashimoto, Diogo Teruo [UNESP]Oliveira, Claudio [UNESP]Porto-Foresti, Fabio [UNESP]Foresti, Fausto [UNESP]2018-11-26T16:33:09Z2018-11-26T16:33:09Z2016-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1407-1418application/pdfhttp://dx.doi.org/10.1007/s00438-016-1195-yMolecular Genetics And Genomics. Heidelberg: Springer Heidelberg, v. 291, n. 3, p. 1407-1418, 2016.1617-4615http://hdl.handle.net/11449/16153710.1007/s00438-016-1195-yWOS:000376509100029WOS000376509100029.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular Genetics And Genomics1,168info:eu-repo/semantics/openAccess2024-04-23T15:23:49Zoai:repositorio.unesp.br:11449/161537Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-23T15:23:49Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Origin of B chromosomes in the genus Astyanax (Characiformes, Characidae) and the limits of chromosome painting |
title |
Origin of B chromosomes in the genus Astyanax (Characiformes, Characidae) and the limits of chromosome painting |
spellingShingle |
Origin of B chromosomes in the genus Astyanax (Characiformes, Characidae) and the limits of chromosome painting Silva, Duilio M. Z. de A. [UNESP] B chromosome rDNA Satellite DNA Microsatellites Chromosome painting |
title_short |
Origin of B chromosomes in the genus Astyanax (Characiformes, Characidae) and the limits of chromosome painting |
title_full |
Origin of B chromosomes in the genus Astyanax (Characiformes, Characidae) and the limits of chromosome painting |
title_fullStr |
Origin of B chromosomes in the genus Astyanax (Characiformes, Characidae) and the limits of chromosome painting |
title_full_unstemmed |
Origin of B chromosomes in the genus Astyanax (Characiformes, Characidae) and the limits of chromosome painting |
title_sort |
Origin of B chromosomes in the genus Astyanax (Characiformes, Characidae) and the limits of chromosome painting |
author |
Silva, Duilio M. Z. de A. [UNESP] |
author_facet |
Silva, Duilio M. Z. de A. [UNESP] Daniel, Sandro Natal [UNESP] Camacho, Juan Pedro M. Utsunomia, Ricardo [UNESP] Ruiz-Ruano, Francisco J. Penitente, Manolo [UNESP] Pansonato-Alves, Jose Carlos [UNESP] Hashimoto, Diogo Teruo [UNESP] Oliveira, Claudio [UNESP] Porto-Foresti, Fabio [UNESP] Foresti, Fausto [UNESP] |
author_role |
author |
author2 |
Daniel, Sandro Natal [UNESP] Camacho, Juan Pedro M. Utsunomia, Ricardo [UNESP] Ruiz-Ruano, Francisco J. Penitente, Manolo [UNESP] Pansonato-Alves, Jose Carlos [UNESP] Hashimoto, Diogo Teruo [UNESP] Oliveira, Claudio [UNESP] Porto-Foresti, Fabio [UNESP] Foresti, Fausto [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Granada |
dc.contributor.author.fl_str_mv |
Silva, Duilio M. Z. de A. [UNESP] Daniel, Sandro Natal [UNESP] Camacho, Juan Pedro M. Utsunomia, Ricardo [UNESP] Ruiz-Ruano, Francisco J. Penitente, Manolo [UNESP] Pansonato-Alves, Jose Carlos [UNESP] Hashimoto, Diogo Teruo [UNESP] Oliveira, Claudio [UNESP] Porto-Foresti, Fabio [UNESP] Foresti, Fausto [UNESP] |
dc.subject.por.fl_str_mv |
B chromosome rDNA Satellite DNA Microsatellites Chromosome painting |
topic |
B chromosome rDNA Satellite DNA Microsatellites Chromosome painting |
description |
Eukaryote genomes are frequently burdened with the presence of supernumerary (B) chromosomes. Their origin is frequently investigated by chromosome painting, under the hypothesis that sharing the repetitive DNA sequences contained in the painting probes is a sign of common descent. However, the intragenomic mobility of many anonymous DNA sequences contained in these probes (e.g., transposable elements) adds high uncertainty to this conclusion. Here we test the validity of chromosome painting to investigate B chromosome origin by comparing its results for seven B chromosome types in two fish species genus Astyanax, with those obtained (1) by means of the physical mapping of 18S ribosomal DNA (rDNA), H1 histone genes, the As51 satellite DNA and the (AC)(15) microsatellite, and (2) by comparing the nucleotide sequence of one of these families (ITS regions from ribosomal DNA) between genomic DNA from B-lacking individuals in both species and the microdissected DNA from two metacentric B chromosomes found in these same species. Intra- and inter-specific painting suggested that all B chromosomes that were assayed shared homologous DNA sequences among them, as well as with a variable number of A chromosomes in each species. This finding would be consistent with a common origin for all seven B chromosomes analyzed. By contrast, the physical mapping of repetitive DNA sequences failed to give support to this hypothesis, as no more than two B-types shared a given repetitive DNA. Finally, sequence analysis of the ITS regions suggested that at least some of the B chromosomes could have had a common origin. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 2018-11-26T16:33:09Z 2018-11-26T16:33:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00438-016-1195-y Molecular Genetics And Genomics. Heidelberg: Springer Heidelberg, v. 291, n. 3, p. 1407-1418, 2016. 1617-4615 http://hdl.handle.net/11449/161537 10.1007/s00438-016-1195-y WOS:000376509100029 WOS000376509100029.pdf |
url |
http://dx.doi.org/10.1007/s00438-016-1195-y http://hdl.handle.net/11449/161537 |
identifier_str_mv |
Molecular Genetics And Genomics. Heidelberg: Springer Heidelberg, v. 291, n. 3, p. 1407-1418, 2016. 1617-4615 10.1007/s00438-016-1195-y WOS:000376509100029 WOS000376509100029.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecular Genetics And Genomics 1,168 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1407-1418 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650049455947776 |