Myotendinous Junction: Exercise Protocols Can Positively Influence Their Development in Rats
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/biomedicines10020480 http://hdl.handle.net/11449/223543 |
Resumo: | The myotendinous junction (MTJ) is an interface that different stimuli alter their morphol-ogy. One of the main stimuli to promote alterations in the MTJ morphology is physical exercise. The present study aimed to investigate the morphology and molecular MTJ adaptations of biceps brachii muscle in adult Wistar rats submitted to different ladder-based protocols. Forty Wistar rats (90 days old) were divided into four groups: Sedentary (S), Climbing (C), Overload Climbing (OC), Climbing, and Overload Climbing (COC). The results of light microscopy demonstrated the cell and collagen tissue reorganization in the experimental groups. The sarcomeres lengths of different regions showed a particular development according to the specific protocols. The sarcoplasmic invagina-tions and evaginations demonstrated positive increases that promoted the myotendinous interface development. In the extracellular matrix, the structures presented an increase principally in the COC group. Finally, the immunofluorescence analysis showed the telocytes disposition adjacent to the MTJ region in all experimental groups, revealing their network organization. Thus, we concluded that the different protocols contributed to the morphological adaptations with beneficial effects in distinct ways of tissue and cellular development and can be used as a model for MTJ remodeling to future proteomic and genetic analysis. |
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Myotendinous Junction: Exercise Protocols Can Positively Influence Their Development in RatsBasal laminaMorphometryMyotendinous junctionTelocyteThe myotendinous junction (MTJ) is an interface that different stimuli alter their morphol-ogy. One of the main stimuli to promote alterations in the MTJ morphology is physical exercise. The present study aimed to investigate the morphology and molecular MTJ adaptations of biceps brachii muscle in adult Wistar rats submitted to different ladder-based protocols. Forty Wistar rats (90 days old) were divided into four groups: Sedentary (S), Climbing (C), Overload Climbing (OC), Climbing, and Overload Climbing (COC). The results of light microscopy demonstrated the cell and collagen tissue reorganization in the experimental groups. The sarcomeres lengths of different regions showed a particular development according to the specific protocols. The sarcoplasmic invagina-tions and evaginations demonstrated positive increases that promoted the myotendinous interface development. In the extracellular matrix, the structures presented an increase principally in the COC group. Finally, the immunofluorescence analysis showed the telocytes disposition adjacent to the MTJ region in all experimental groups, revealing their network organization. Thus, we concluded that the different protocols contributed to the morphological adaptations with beneficial effects in distinct ways of tissue and cellular development and can be used as a model for MTJ remodeling to future proteomic and genetic analysis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Laboratory of Morphology and Physical Activity—LAMAF Institute of Biosciences—IB São Paulo State University—UNESP, 24A, No.1515, SPLaboratory of Morphology and Physical Activity—LAMAF Institute of Biosciences—IB São Paulo State University—UNESP, 24A, No.1515, SPFAPESP: #2018/09199-8CAPES: 001FAPESP: 2017/12525-1FAPESP: 2017/21977-3Universidade Estadual Paulista (UNESP)Neto, Jurandyr Pimentel [UNESP]Rocha-Braga, Lara Caetano [UNESP]Dos Santos Jacob, Carolina [UNESP]Tomiate, André Neri [UNESP]Ciena, Adriano Polican [UNESP]2022-04-28T19:51:20Z2022-04-28T19:51:20Z2022-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/biomedicines10020480Biomedicines, v. 10, n. 2, 2022.2227-9059http://hdl.handle.net/11449/22354310.3390/biomedicines100204802-s2.0-85125497810Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomedicinesinfo:eu-repo/semantics/openAccess2022-04-28T19:51:20Zoai:repositorio.unesp.br:11449/223543Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:59:13.511348Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Myotendinous Junction: Exercise Protocols Can Positively Influence Their Development in Rats |
title |
Myotendinous Junction: Exercise Protocols Can Positively Influence Their Development in Rats |
spellingShingle |
Myotendinous Junction: Exercise Protocols Can Positively Influence Their Development in Rats Neto, Jurandyr Pimentel [UNESP] Basal lamina Morphometry Myotendinous junction Telocyte |
title_short |
Myotendinous Junction: Exercise Protocols Can Positively Influence Their Development in Rats |
title_full |
Myotendinous Junction: Exercise Protocols Can Positively Influence Their Development in Rats |
title_fullStr |
Myotendinous Junction: Exercise Protocols Can Positively Influence Their Development in Rats |
title_full_unstemmed |
Myotendinous Junction: Exercise Protocols Can Positively Influence Their Development in Rats |
title_sort |
Myotendinous Junction: Exercise Protocols Can Positively Influence Their Development in Rats |
author |
Neto, Jurandyr Pimentel [UNESP] |
author_facet |
Neto, Jurandyr Pimentel [UNESP] Rocha-Braga, Lara Caetano [UNESP] Dos Santos Jacob, Carolina [UNESP] Tomiate, André Neri [UNESP] Ciena, Adriano Polican [UNESP] |
author_role |
author |
author2 |
Rocha-Braga, Lara Caetano [UNESP] Dos Santos Jacob, Carolina [UNESP] Tomiate, André Neri [UNESP] Ciena, Adriano Polican [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Neto, Jurandyr Pimentel [UNESP] Rocha-Braga, Lara Caetano [UNESP] Dos Santos Jacob, Carolina [UNESP] Tomiate, André Neri [UNESP] Ciena, Adriano Polican [UNESP] |
dc.subject.por.fl_str_mv |
Basal lamina Morphometry Myotendinous junction Telocyte |
topic |
Basal lamina Morphometry Myotendinous junction Telocyte |
description |
The myotendinous junction (MTJ) is an interface that different stimuli alter their morphol-ogy. One of the main stimuli to promote alterations in the MTJ morphology is physical exercise. The present study aimed to investigate the morphology and molecular MTJ adaptations of biceps brachii muscle in adult Wistar rats submitted to different ladder-based protocols. Forty Wistar rats (90 days old) were divided into four groups: Sedentary (S), Climbing (C), Overload Climbing (OC), Climbing, and Overload Climbing (COC). The results of light microscopy demonstrated the cell and collagen tissue reorganization in the experimental groups. The sarcomeres lengths of different regions showed a particular development according to the specific protocols. The sarcoplasmic invagina-tions and evaginations demonstrated positive increases that promoted the myotendinous interface development. In the extracellular matrix, the structures presented an increase principally in the COC group. Finally, the immunofluorescence analysis showed the telocytes disposition adjacent to the MTJ region in all experimental groups, revealing their network organization. Thus, we concluded that the different protocols contributed to the morphological adaptations with beneficial effects in distinct ways of tissue and cellular development and can be used as a model for MTJ remodeling to future proteomic and genetic analysis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:51:20Z 2022-04-28T19:51:20Z 2022-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/biomedicines10020480 Biomedicines, v. 10, n. 2, 2022. 2227-9059 http://hdl.handle.net/11449/223543 10.3390/biomedicines10020480 2-s2.0-85125497810 |
url |
http://dx.doi.org/10.3390/biomedicines10020480 http://hdl.handle.net/11449/223543 |
identifier_str_mv |
Biomedicines, v. 10, n. 2, 2022. 2227-9059 10.3390/biomedicines10020480 2-s2.0-85125497810 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biomedicines |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128444213493760 |