Applications of polymerase chain reaction for the detection of equine Leishmania sp. Infection

Detalhes bibliográficos
Autor(a) principal: Escobar, Taiane Acunha
Data de Publicação: 2020
Outros Autores: Döwich, Gabriela, Cantele, Leticia Carvalho, Góss, Geórgia Camargo, Porciúncula, Marcelo Lameiro, Leite, Carla Teixeira [UNESP], Zuravski, Luísa, Duarte, Claudia Acosta, Lübeck, Irina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.5433/1679-0359.2020v41n1p199
http://hdl.handle.net/11449/198363
Resumo: Leishmaniasis is a neglected zoonotic disease caused by a variety of pathogenic Leishmania species. In the New World, especially in Brazil, visceral leishmaniasis (VL) is caused by Leishmania infantum. The pathogen can infect several animal species including dogs, foxes, rodents, primates, felines, equines and humans. Dogs act as the primary domestic reservoirs. This study aimed to use polymerase chain reaction (PCR) for detecting Leishmania infection in horses living in a canine visceral leishmaniasis (CVL) endemic region. DNA samples from horse peripheral blood were used to perform PCR. Templates were amplified using primers for the kinetoplast DNA (kDNA) minicircles, which were able to detect different species of Leishmania. In addition, primers for internal transcribed spacer (ITS) of ribosomal DNA were used for detection of Trypanosomatidae sp. Amongst the 75 (39%) positive PCR samples from total 192 samples, 21 samples were positive for kDNA and 63 samples were positive for either ITS, ITS1, or ITS2 gene markers. The kDNA PCR and sequencing allowed the detection of L. infantum in horse blood samples. To our knowledge, this is the first report of equine infection with L. infantum in Southern Brazil. These results proved that L. infantum could also infect horses in addition to humans and dogs, as well as in European countries. This conclusion emphasizes the urgent need to follow up investigation of the infection in these animals.
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spelling Applications of polymerase chain reaction for the detection of equine Leishmania sp. InfectionDiagnostic methodsHorsesInfectionLeishmania infantumLeishmaniasis is a neglected zoonotic disease caused by a variety of pathogenic Leishmania species. In the New World, especially in Brazil, visceral leishmaniasis (VL) is caused by Leishmania infantum. The pathogen can infect several animal species including dogs, foxes, rodents, primates, felines, equines and humans. Dogs act as the primary domestic reservoirs. This study aimed to use polymerase chain reaction (PCR) for detecting Leishmania infection in horses living in a canine visceral leishmaniasis (CVL) endemic region. DNA samples from horse peripheral blood were used to perform PCR. Templates were amplified using primers for the kinetoplast DNA (kDNA) minicircles, which were able to detect different species of Leishmania. In addition, primers for internal transcribed spacer (ITS) of ribosomal DNA were used for detection of Trypanosomatidae sp. Amongst the 75 (39%) positive PCR samples from total 192 samples, 21 samples were positive for kDNA and 63 samples were positive for either ITS, ITS1, or ITS2 gene markers. The kDNA PCR and sequencing allowed the detection of L. infantum in horse blood samples. To our knowledge, this is the first report of equine infection with L. infantum in Southern Brazil. These results proved that L. infantum could also infect horses in addition to humans and dogs, as well as in European countries. This conclusion emphasizes the urgent need to follow up investigation of the infection in these animals.North Carolina Biotechnology CenterUniversidade Federal do Rio Grande do SulUniversidade Federal do PampaPrograma de Pós-Graduação em Bioquímica Universidade Federal do Pampa UNIPAMPAPrograma de Pós-Graduação em Ciência Animal UNIPAMPAGraduação em Medicina Veterinária UNIPAMPACurso de Medicina Veterinária Universidade da Região da Campanha URCAMPMedicina Veterinária Programa de Pós-Graduação em Medicina Veterinária Universidade Estadual Paulista UNESPCurso de Graduação em Medicina Veterinária UNIPAMPAMedicina Veterinária Programa de Pós-Graduação em Medicina Veterinária Universidade Estadual Paulista UNESPUNIPAMPAURCAMPUniversidade Estadual Paulista (Unesp)Escobar, Taiane AcunhaDöwich, GabrielaCantele, Leticia CarvalhoGóss, Geórgia CamargoPorciúncula, Marcelo LameiroLeite, Carla Teixeira [UNESP]Zuravski, LuísaDuarte, Claudia AcostaLübeck, Irina2020-12-12T01:10:43Z2020-12-12T01:10:43Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article199-211http://dx.doi.org/10.5433/1679-0359.2020v41n1p199Semina:Ciencias Agrarias, v. 41, n. 1, p. 199-211, 2020.1679-03591676-546Xhttp://hdl.handle.net/11449/19836310.5433/1679-0359.2020v41n1p1992-s2.0-85077389145Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSemina:Ciencias Agrariasinfo:eu-repo/semantics/openAccess2021-10-23T10:18:20Zoai:repositorio.unesp.br:11449/198363Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:48:19.966038Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Applications of polymerase chain reaction for the detection of equine Leishmania sp. Infection
title Applications of polymerase chain reaction for the detection of equine Leishmania sp. Infection
spellingShingle Applications of polymerase chain reaction for the detection of equine Leishmania sp. Infection
Escobar, Taiane Acunha
Diagnostic methods
Horses
Infection
Leishmania infantum
title_short Applications of polymerase chain reaction for the detection of equine Leishmania sp. Infection
title_full Applications of polymerase chain reaction for the detection of equine Leishmania sp. Infection
title_fullStr Applications of polymerase chain reaction for the detection of equine Leishmania sp. Infection
title_full_unstemmed Applications of polymerase chain reaction for the detection of equine Leishmania sp. Infection
title_sort Applications of polymerase chain reaction for the detection of equine Leishmania sp. Infection
author Escobar, Taiane Acunha
author_facet Escobar, Taiane Acunha
Döwich, Gabriela
Cantele, Leticia Carvalho
Góss, Geórgia Camargo
Porciúncula, Marcelo Lameiro
Leite, Carla Teixeira [UNESP]
Zuravski, Luísa
Duarte, Claudia Acosta
Lübeck, Irina
author_role author
author2 Döwich, Gabriela
Cantele, Leticia Carvalho
Góss, Geórgia Camargo
Porciúncula, Marcelo Lameiro
Leite, Carla Teixeira [UNESP]
Zuravski, Luísa
Duarte, Claudia Acosta
Lübeck, Irina
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UNIPAMPA
URCAMP
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Escobar, Taiane Acunha
Döwich, Gabriela
Cantele, Leticia Carvalho
Góss, Geórgia Camargo
Porciúncula, Marcelo Lameiro
Leite, Carla Teixeira [UNESP]
Zuravski, Luísa
Duarte, Claudia Acosta
Lübeck, Irina
dc.subject.por.fl_str_mv Diagnostic methods
Horses
Infection
Leishmania infantum
topic Diagnostic methods
Horses
Infection
Leishmania infantum
description Leishmaniasis is a neglected zoonotic disease caused by a variety of pathogenic Leishmania species. In the New World, especially in Brazil, visceral leishmaniasis (VL) is caused by Leishmania infantum. The pathogen can infect several animal species including dogs, foxes, rodents, primates, felines, equines and humans. Dogs act as the primary domestic reservoirs. This study aimed to use polymerase chain reaction (PCR) for detecting Leishmania infection in horses living in a canine visceral leishmaniasis (CVL) endemic region. DNA samples from horse peripheral blood were used to perform PCR. Templates were amplified using primers for the kinetoplast DNA (kDNA) minicircles, which were able to detect different species of Leishmania. In addition, primers for internal transcribed spacer (ITS) of ribosomal DNA were used for detection of Trypanosomatidae sp. Amongst the 75 (39%) positive PCR samples from total 192 samples, 21 samples were positive for kDNA and 63 samples were positive for either ITS, ITS1, or ITS2 gene markers. The kDNA PCR and sequencing allowed the detection of L. infantum in horse blood samples. To our knowledge, this is the first report of equine infection with L. infantum in Southern Brazil. These results proved that L. infantum could also infect horses in addition to humans and dogs, as well as in European countries. This conclusion emphasizes the urgent need to follow up investigation of the infection in these animals.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:10:43Z
2020-12-12T01:10:43Z
2020-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5433/1679-0359.2020v41n1p199
Semina:Ciencias Agrarias, v. 41, n. 1, p. 199-211, 2020.
1679-0359
1676-546X
http://hdl.handle.net/11449/198363
10.5433/1679-0359.2020v41n1p199
2-s2.0-85077389145
url http://dx.doi.org/10.5433/1679-0359.2020v41n1p199
http://hdl.handle.net/11449/198363
identifier_str_mv Semina:Ciencias Agrarias, v. 41, n. 1, p. 199-211, 2020.
1679-0359
1676-546X
10.5433/1679-0359.2020v41n1p199
2-s2.0-85077389145
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Semina:Ciencias Agrarias
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 199-211
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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