Deregulation of VEGFR-2 and PDGFR expression and microvascular density in a triple-negative model of canine malignant mammary tumors with lymph node or lung metastasis

Detalhes bibliográficos
Autor(a) principal: dos Anjos, Denner Santos
Data de Publicação: 2019
Outros Autores: Vital, Aline Fernandes, Lainetti, Patrícia de Faria [UNESP], Leis-Filho, Antonio Fernando [UNESP], Dalmolin, Fabiola, Elias, Fabiana, Calazans, Sabryna Gouveia, Fonseca-Alves, Carlos Eduardo [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/VETSCI6010003
http://hdl.handle.net/11449/189453
Resumo: Canine mammary tumors (CMT) represent the most common cancer in noncastrated female dogs. Interestingly, triple-negative tumors are the most common molecular subtype in female dogs. In this study, we proposed to evaluate the expression of vascular endothelial growth factor receptor 2 (VEGFR-2), Platelet-derived growth factor receptor (PDGFR), and microvascular density (MVD) in a group of metastatic and nonmetastatic triple-negative CMT and compare the expression based on clinical parameters. Twenty-six female dogs with triple-negative mammary tumors were divided into three groups: nonmetastatic tumors (NMT) (n = 11), tumors with lymph node metastasis (LNM) (n = 10), and tumors with lung metastasis (LM) (n = 5). We observed increased VEGFR-2 expression in LNM compared with NMT and a positive correlation between tumor grade and VEGFR-2 expression. A positive correlation was noted between VEGFR-2 and PDGFR expression. Regarding microvascular density (MVD), we identified a higher number of vessels in primary tumors with lymph node metastasis and lung metastasis compared with tumors with no metastasis. The primary tumors with lung metastasis exhibited an increased MVD compared with carcinoma with lymph node metastasis. Overall, our results suggest a deregulation of VEGFR-2 and PDGFR and high MVD in metastatic tumors, indicating a role for angiogenesis in tumor progression.
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spelling Deregulation of VEGFR-2 and PDGFR expression and microvascular density in a triple-negative model of canine malignant mammary tumors with lymph node or lung metastasisAngiogenesisMammary neoplasmMetastasisCanine mammary tumors (CMT) represent the most common cancer in noncastrated female dogs. Interestingly, triple-negative tumors are the most common molecular subtype in female dogs. In this study, we proposed to evaluate the expression of vascular endothelial growth factor receptor 2 (VEGFR-2), Platelet-derived growth factor receptor (PDGFR), and microvascular density (MVD) in a group of metastatic and nonmetastatic triple-negative CMT and compare the expression based on clinical parameters. Twenty-six female dogs with triple-negative mammary tumors were divided into three groups: nonmetastatic tumors (NMT) (n = 11), tumors with lymph node metastasis (LNM) (n = 10), and tumors with lung metastasis (LM) (n = 5). We observed increased VEGFR-2 expression in LNM compared with NMT and a positive correlation between tumor grade and VEGFR-2 expression. A positive correlation was noted between VEGFR-2 and PDGFR expression. Regarding microvascular density (MVD), we identified a higher number of vessels in primary tumors with lymph node metastasis and lung metastasis compared with tumors with no metastasis. The primary tumors with lung metastasis exhibited an increased MVD compared with carcinoma with lymph node metastasis. Overall, our results suggest a deregulation of VEGFR-2 and PDGFR and high MVD in metastatic tumors, indicating a role for angiogenesis in tumor progression.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Veterinary Science Graduate Program University of Franca (UNIFRAN)Department of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University-UNESPSuperintendencia Unidade Hospitalar Veterinaria Universitaria Federal University of the Fronteira SulDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University-UNESPUniversity of Franca (UNIFRAN)Universidade Estadual Paulista (Unesp)Federal University of the Fronteira Suldos Anjos, Denner SantosVital, Aline FernandesLainetti, Patrícia de Faria [UNESP]Leis-Filho, Antonio Fernando [UNESP]Dalmolin, FabiolaElias, FabianaCalazans, Sabryna GouveiaFonseca-Alves, Carlos Eduardo [UNESP]2019-10-06T16:41:12Z2019-10-06T16:41:12Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/VETSCI6010003Veterinary Sciences, v. 6, n. 1, 2019.2306-7381http://hdl.handle.net/11449/18945310.3390/VETSCI60100032-s2.0-85069768312Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Sciencesinfo:eu-repo/semantics/openAccess2021-10-23T12:39:45Zoai:repositorio.unesp.br:11449/189453Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:33:06.561104Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Deregulation of VEGFR-2 and PDGFR expression and microvascular density in a triple-negative model of canine malignant mammary tumors with lymph node or lung metastasis
title Deregulation of VEGFR-2 and PDGFR expression and microvascular density in a triple-negative model of canine malignant mammary tumors with lymph node or lung metastasis
spellingShingle Deregulation of VEGFR-2 and PDGFR expression and microvascular density in a triple-negative model of canine malignant mammary tumors with lymph node or lung metastasis
dos Anjos, Denner Santos
Angiogenesis
Mammary neoplasm
Metastasis
title_short Deregulation of VEGFR-2 and PDGFR expression and microvascular density in a triple-negative model of canine malignant mammary tumors with lymph node or lung metastasis
title_full Deregulation of VEGFR-2 and PDGFR expression and microvascular density in a triple-negative model of canine malignant mammary tumors with lymph node or lung metastasis
title_fullStr Deregulation of VEGFR-2 and PDGFR expression and microvascular density in a triple-negative model of canine malignant mammary tumors with lymph node or lung metastasis
title_full_unstemmed Deregulation of VEGFR-2 and PDGFR expression and microvascular density in a triple-negative model of canine malignant mammary tumors with lymph node or lung metastasis
title_sort Deregulation of VEGFR-2 and PDGFR expression and microvascular density in a triple-negative model of canine malignant mammary tumors with lymph node or lung metastasis
author dos Anjos, Denner Santos
author_facet dos Anjos, Denner Santos
Vital, Aline Fernandes
Lainetti, Patrícia de Faria [UNESP]
Leis-Filho, Antonio Fernando [UNESP]
Dalmolin, Fabiola
Elias, Fabiana
Calazans, Sabryna Gouveia
Fonseca-Alves, Carlos Eduardo [UNESP]
author_role author
author2 Vital, Aline Fernandes
Lainetti, Patrícia de Faria [UNESP]
Leis-Filho, Antonio Fernando [UNESP]
Dalmolin, Fabiola
Elias, Fabiana
Calazans, Sabryna Gouveia
Fonseca-Alves, Carlos Eduardo [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University of Franca (UNIFRAN)
Universidade Estadual Paulista (Unesp)
Federal University of the Fronteira Sul
dc.contributor.author.fl_str_mv dos Anjos, Denner Santos
Vital, Aline Fernandes
Lainetti, Patrícia de Faria [UNESP]
Leis-Filho, Antonio Fernando [UNESP]
Dalmolin, Fabiola
Elias, Fabiana
Calazans, Sabryna Gouveia
Fonseca-Alves, Carlos Eduardo [UNESP]
dc.subject.por.fl_str_mv Angiogenesis
Mammary neoplasm
Metastasis
topic Angiogenesis
Mammary neoplasm
Metastasis
description Canine mammary tumors (CMT) represent the most common cancer in noncastrated female dogs. Interestingly, triple-negative tumors are the most common molecular subtype in female dogs. In this study, we proposed to evaluate the expression of vascular endothelial growth factor receptor 2 (VEGFR-2), Platelet-derived growth factor receptor (PDGFR), and microvascular density (MVD) in a group of metastatic and nonmetastatic triple-negative CMT and compare the expression based on clinical parameters. Twenty-six female dogs with triple-negative mammary tumors were divided into three groups: nonmetastatic tumors (NMT) (n = 11), tumors with lymph node metastasis (LNM) (n = 10), and tumors with lung metastasis (LM) (n = 5). We observed increased VEGFR-2 expression in LNM compared with NMT and a positive correlation between tumor grade and VEGFR-2 expression. A positive correlation was noted between VEGFR-2 and PDGFR expression. Regarding microvascular density (MVD), we identified a higher number of vessels in primary tumors with lymph node metastasis and lung metastasis compared with tumors with no metastasis. The primary tumors with lung metastasis exhibited an increased MVD compared with carcinoma with lymph node metastasis. Overall, our results suggest a deregulation of VEGFR-2 and PDGFR and high MVD in metastatic tumors, indicating a role for angiogenesis in tumor progression.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:41:12Z
2019-10-06T16:41:12Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/VETSCI6010003
Veterinary Sciences, v. 6, n. 1, 2019.
2306-7381
http://hdl.handle.net/11449/189453
10.3390/VETSCI6010003
2-s2.0-85069768312
url http://dx.doi.org/10.3390/VETSCI6010003
http://hdl.handle.net/11449/189453
identifier_str_mv Veterinary Sciences, v. 6, n. 1, 2019.
2306-7381
10.3390/VETSCI6010003
2-s2.0-85069768312
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Sciences
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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