Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria

Detalhes bibliográficos
Autor(a) principal: Husain, Fohad M.
Data de Publicação: 2015
Outros Autores: Ahmad, Iqbal, Khan, Mohammad S., Ahmad, Ejaz [UNESP], Tahseen, Qudisa, Khan, Mohd Shahnawaz, Alshabib, Nasser A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://journal.frontiersin.org/article/10.3389/fmicb.2015.00420/abstract
http://hdl.handle.net/11449/129681
Resumo: Bacterial quorum sensing (QS) is a density dependent communication system that regulates the expression of certain genes including production of virulence factors in many pathogens. Bioactive plant extract/compounds inhibiting QS regulated gene expression may be a potential candidate as antipathogenic drug. In this study anti-QS activity of peppermint (Menthe piperita) oil was first tested using the Chromobacterium violaceum CVO26 biosensor. Further, the findings of the present investigation revealed that peppermint oil (PMO) at sub-Minimum Inhibitory Concentrations (sub-MICs) strongly interfered with acyl homoserine lactone (AHL) regulated virulence factors and biofilm formation in Pseudomonas aeruginosa and Aeromonas hydrophila. The result of molecular docking analysis attributed the QS inhibitory activity exhibited by PMO to menthol. Assessment of ability of menthol to interfere with QS systems of various Gram-negative pathogens comprising diverse AHL molecules revealed that it reduced the AHL dependent production of violacein, virulence factors, and biofilm formation indicating broad-spectrum anti-QS activity. Using two Escherichia colt biosensors, MG4/pKDT17 and pEAL08-2, we also confirmed that menthol inhibited both the las and pqs QS systems. Further, findings of the in vivo studies with menthol on nematode model Caenorhabditis elegans showed significantly enhanced survival of the nematode. Our data identified menthol as a novel broad spectrum QS inhibitor.
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spelling Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteriaAnti-quorum sensing activityPeppermint oilMentholBiofilmMolecular dockingC. elegansBacterial quorum sensing (QS) is a density dependent communication system that regulates the expression of certain genes including production of virulence factors in many pathogens. Bioactive plant extract/compounds inhibiting QS regulated gene expression may be a potential candidate as antipathogenic drug. In this study anti-QS activity of peppermint (Menthe piperita) oil was first tested using the Chromobacterium violaceum CVO26 biosensor. Further, the findings of the present investigation revealed that peppermint oil (PMO) at sub-Minimum Inhibitory Concentrations (sub-MICs) strongly interfered with acyl homoserine lactone (AHL) regulated virulence factors and biofilm formation in Pseudomonas aeruginosa and Aeromonas hydrophila. The result of molecular docking analysis attributed the QS inhibitory activity exhibited by PMO to menthol. Assessment of ability of menthol to interfere with QS systems of various Gram-negative pathogens comprising diverse AHL molecules revealed that it reduced the AHL dependent production of violacein, virulence factors, and biofilm formation indicating broad-spectrum anti-QS activity. Using two Escherichia colt biosensors, MG4/pKDT17 and pEAL08-2, we also confirmed that menthol inhibited both the las and pqs QS systems. Further, findings of the in vivo studies with menthol on nematode model Caenorhabditis elegans showed significantly enhanced survival of the nematode. Our data identified menthol as a novel broad spectrum QS inhibitor.Deanship of Scientific Research at KSUDepartment of Agricultural Microbiology, Aligarh Muslim University, Aligarh, IndiaDepartment of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi ArabiaDepartment of Zoology, Aligarh Muslim University, Aligarh, IndiaDepartment of Biochemistry, Protein Research Chair, College of Science, King Saud University, Riyadh, Saudi ArabiaSchool of Pharmaceutical Sciences, Sao Paulo State University, Araraquara, BrazilDeanship of Scientific Research at KSU: RGP-215Frontiers Research FoundationAligarh Muslim UniversityKing Saud UniversityUniversidade Estadual Paulista (Unesp)Husain, Fohad M.Ahmad, IqbalKhan, Mohammad S.Ahmad, Ejaz [UNESP]Tahseen, QudisaKhan, Mohd ShahnawazAlshabib, Nasser A.2015-10-22T06:29:19Z2015-10-22T06:29:19Z2015-05-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-12application/pdfhttp://journal.frontiersin.org/article/10.3389/fmicb.2015.00420/abstractFrontiers In Microbiology. Lausanne: Frontiers Research Foundation, v. 6, p. 1-12, 2015.1664-302Xhttp://hdl.handle.net/11449/12968110.3389/fmicb.2015.00420WOS:000355832500001WOS000355832500001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Microbiology4.019info:eu-repo/semantics/openAccess2024-01-25T06:30:17Zoai:repositorio.unesp.br:11449/129681Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:54:41.882439Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria
title Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria
spellingShingle Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria
Husain, Fohad M.
Anti-quorum sensing activity
Peppermint oil
Menthol
Biofilm
Molecular docking
C. elegans
title_short Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria
title_full Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria
title_fullStr Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria
title_full_unstemmed Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria
title_sort Sub-MICs of Mentha piperita essential oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria
author Husain, Fohad M.
author_facet Husain, Fohad M.
Ahmad, Iqbal
Khan, Mohammad S.
Ahmad, Ejaz [UNESP]
Tahseen, Qudisa
Khan, Mohd Shahnawaz
Alshabib, Nasser A.
author_role author
author2 Ahmad, Iqbal
Khan, Mohammad S.
Ahmad, Ejaz [UNESP]
Tahseen, Qudisa
Khan, Mohd Shahnawaz
Alshabib, Nasser A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Aligarh Muslim University
King Saud University
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Husain, Fohad M.
Ahmad, Iqbal
Khan, Mohammad S.
Ahmad, Ejaz [UNESP]
Tahseen, Qudisa
Khan, Mohd Shahnawaz
Alshabib, Nasser A.
dc.subject.por.fl_str_mv Anti-quorum sensing activity
Peppermint oil
Menthol
Biofilm
Molecular docking
C. elegans
topic Anti-quorum sensing activity
Peppermint oil
Menthol
Biofilm
Molecular docking
C. elegans
description Bacterial quorum sensing (QS) is a density dependent communication system that regulates the expression of certain genes including production of virulence factors in many pathogens. Bioactive plant extract/compounds inhibiting QS regulated gene expression may be a potential candidate as antipathogenic drug. In this study anti-QS activity of peppermint (Menthe piperita) oil was first tested using the Chromobacterium violaceum CVO26 biosensor. Further, the findings of the present investigation revealed that peppermint oil (PMO) at sub-Minimum Inhibitory Concentrations (sub-MICs) strongly interfered with acyl homoserine lactone (AHL) regulated virulence factors and biofilm formation in Pseudomonas aeruginosa and Aeromonas hydrophila. The result of molecular docking analysis attributed the QS inhibitory activity exhibited by PMO to menthol. Assessment of ability of menthol to interfere with QS systems of various Gram-negative pathogens comprising diverse AHL molecules revealed that it reduced the AHL dependent production of violacein, virulence factors, and biofilm formation indicating broad-spectrum anti-QS activity. Using two Escherichia colt biosensors, MG4/pKDT17 and pEAL08-2, we also confirmed that menthol inhibited both the las and pqs QS systems. Further, findings of the in vivo studies with menthol on nematode model Caenorhabditis elegans showed significantly enhanced survival of the nematode. Our data identified menthol as a novel broad spectrum QS inhibitor.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-22T06:29:19Z
2015-10-22T06:29:19Z
2015-05-13
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://journal.frontiersin.org/article/10.3389/fmicb.2015.00420/abstract
Frontiers In Microbiology. Lausanne: Frontiers Research Foundation, v. 6, p. 1-12, 2015.
1664-302X
http://hdl.handle.net/11449/129681
10.3389/fmicb.2015.00420
WOS:000355832500001
WOS000355832500001.pdf
url http://journal.frontiersin.org/article/10.3389/fmicb.2015.00420/abstract
http://hdl.handle.net/11449/129681
identifier_str_mv Frontiers In Microbiology. Lausanne: Frontiers Research Foundation, v. 6, p. 1-12, 2015.
1664-302X
10.3389/fmicb.2015.00420
WOS:000355832500001
WOS000355832500001.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers In Microbiology
4.019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-12
application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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