Oxidative and Inflammatory Imbalance in Placenta and Kidney of sFlt1-Induced Early-Onset Preeclampsia Rat Model
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/antiox11081608 http://hdl.handle.net/11449/240774 |
Resumo: | Preeclampsia (PE) is a pregnancy-specific disorder characterized by the new onset of hypertension plus proteinuria and/or end-organ dysfunction. Here, we investigate the role of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase system as a major component of reactive oxygen species generation, in a rodent model of early-onset preeclampsia induced by excess sFlt1 (soluble fms-like tyrosine kinase 1). Placenta and kidney samples were obtained from normal pregnant and PE rats to measure the sFlt1/PlGF (placental growth factor) ratio in addition to oxidative stress-related parameters, including the activities and expressions of NADPH oxidase isoforms (NOX1, NOX2, and NOX4), components of nitric oxide (NO) metabolism, and antioxidant enzymes. Peroxisome proliferator-activated receptors (PPARα, PPARγ) and cytokines IL1β, IL3, IL6, IL10, and IL18 were also measured to evaluate the inflammation status in our experimental setting. Excessive O2●− production was found in rats that were treated with sFlt1; interestingly, this alteration appears to be mediated mainly by NOX2 in the placenta and by NOX4 in the kidney. Altered NO metabolism and antioxidant defense systems, together with mitochondrial dysfunction, were observed in this model of PE. Preeclamptic animals also exhibited overexpression of proinflammatory biomarkers as well as increased collagen deposition. Our results highlight the role of NADPH oxidase in mediating oxidative stress and possibly inflammatory processes in the placenta and kidney of an sFlt1-based model of early-onset preeclampsia. |
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Oxidative and Inflammatory Imbalance in Placenta and Kidney of sFlt1-Induced Early-Onset Preeclampsia Rat ModelinflammationkidneyNADPH oxidasenitric oxideoxidative stressplacentapreeclampsiasFlt1Preeclampsia (PE) is a pregnancy-specific disorder characterized by the new onset of hypertension plus proteinuria and/or end-organ dysfunction. Here, we investigate the role of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase system as a major component of reactive oxygen species generation, in a rodent model of early-onset preeclampsia induced by excess sFlt1 (soluble fms-like tyrosine kinase 1). Placenta and kidney samples were obtained from normal pregnant and PE rats to measure the sFlt1/PlGF (placental growth factor) ratio in addition to oxidative stress-related parameters, including the activities and expressions of NADPH oxidase isoforms (NOX1, NOX2, and NOX4), components of nitric oxide (NO) metabolism, and antioxidant enzymes. Peroxisome proliferator-activated receptors (PPARα, PPARγ) and cytokines IL1β, IL3, IL6, IL10, and IL18 were also measured to evaluate the inflammation status in our experimental setting. Excessive O2●− production was found in rats that were treated with sFlt1; interestingly, this alteration appears to be mediated mainly by NOX2 in the placenta and by NOX4 in the kidney. Altered NO metabolism and antioxidant defense systems, together with mitochondrial dysfunction, were observed in this model of PE. Preeclamptic animals also exhibited overexpression of proinflammatory biomarkers as well as increased collagen deposition. Our results highlight the role of NADPH oxidase in mediating oxidative stress and possibly inflammatory processes in the placenta and kidney of an sFlt1-based model of early-onset preeclampsia.Ministerio de Ciencia, Innovación y UniversidadesDepartamento de Fisiología Facultad de Farmacia Universidad de SevillaEpidemiología Clínica y Riesgo Cardiovascular Instituto de Biomedicina de Sevilla (IBIS) Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de SevillaCellular and Molecular Physiology Laboratory (CMPL) Department of Obstetrics Division of Obstetrics and Gynaecology Pontificia Universidad Católica de ChileMedical School (Faculty of Medicine) São Paulo State University (UNESP)University of Queensland Centre for Clinical Research (UQCCR) Faculty of Medicine and Biomedical Sciences University of QueenslandDepartment of Pathology and Medical Biology University of Groningen University Medical Center Groningen (UMCG)Tecnológico de Monterrey Eutra The Institute for Obesity Research (IOR) School of Medicine and Health Sciences, Nuevo LeónDepartamento de Medicina Facultad de Medicina Universidad de SevillaMedical School (Faculty of Medicine) São Paulo State University (UNESP)Ministerio de Ciencia, Innovación y Universidades: FPU17/03465Universidad de SevillaHospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de SevillaPontificia Universidad Católica de ChileUniversidade Estadual Paulista (UNESP)University of QueenslandUniversity Medical Center Groningen (UMCG)School of Medicine and Health SciencesSantana-Garrido, ÁlvaroReyes-Goya, ClaudiaEspinosa-Martín, PabloSobrevia, Luis [UNESP]Beltrán, Luis M.Vázquez, Carmen M.Mate, Alfonso2023-03-01T20:32:19Z2023-03-01T20:32:19Z2022-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/antiox11081608Antioxidants, v. 11, n. 8, 2022.2076-3921http://hdl.handle.net/11449/24077410.3390/antiox110816082-s2.0-85137354426Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAntioxidantsinfo:eu-repo/semantics/openAccess2023-03-01T20:32:19Zoai:repositorio.unesp.br:11449/240774Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:23:46.642207Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Oxidative and Inflammatory Imbalance in Placenta and Kidney of sFlt1-Induced Early-Onset Preeclampsia Rat Model |
title |
Oxidative and Inflammatory Imbalance in Placenta and Kidney of sFlt1-Induced Early-Onset Preeclampsia Rat Model |
spellingShingle |
Oxidative and Inflammatory Imbalance in Placenta and Kidney of sFlt1-Induced Early-Onset Preeclampsia Rat Model Santana-Garrido, Álvaro inflammation kidney NADPH oxidase nitric oxide oxidative stress placenta preeclampsia sFlt1 |
title_short |
Oxidative and Inflammatory Imbalance in Placenta and Kidney of sFlt1-Induced Early-Onset Preeclampsia Rat Model |
title_full |
Oxidative and Inflammatory Imbalance in Placenta and Kidney of sFlt1-Induced Early-Onset Preeclampsia Rat Model |
title_fullStr |
Oxidative and Inflammatory Imbalance in Placenta and Kidney of sFlt1-Induced Early-Onset Preeclampsia Rat Model |
title_full_unstemmed |
Oxidative and Inflammatory Imbalance in Placenta and Kidney of sFlt1-Induced Early-Onset Preeclampsia Rat Model |
title_sort |
Oxidative and Inflammatory Imbalance in Placenta and Kidney of sFlt1-Induced Early-Onset Preeclampsia Rat Model |
author |
Santana-Garrido, Álvaro |
author_facet |
Santana-Garrido, Álvaro Reyes-Goya, Claudia Espinosa-Martín, Pablo Sobrevia, Luis [UNESP] Beltrán, Luis M. Vázquez, Carmen M. Mate, Alfonso |
author_role |
author |
author2 |
Reyes-Goya, Claudia Espinosa-Martín, Pablo Sobrevia, Luis [UNESP] Beltrán, Luis M. Vázquez, Carmen M. Mate, Alfonso |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidad de Sevilla Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de Sevilla Pontificia Universidad Católica de Chile Universidade Estadual Paulista (UNESP) University of Queensland University Medical Center Groningen (UMCG) School of Medicine and Health Sciences |
dc.contributor.author.fl_str_mv |
Santana-Garrido, Álvaro Reyes-Goya, Claudia Espinosa-Martín, Pablo Sobrevia, Luis [UNESP] Beltrán, Luis M. Vázquez, Carmen M. Mate, Alfonso |
dc.subject.por.fl_str_mv |
inflammation kidney NADPH oxidase nitric oxide oxidative stress placenta preeclampsia sFlt1 |
topic |
inflammation kidney NADPH oxidase nitric oxide oxidative stress placenta preeclampsia sFlt1 |
description |
Preeclampsia (PE) is a pregnancy-specific disorder characterized by the new onset of hypertension plus proteinuria and/or end-organ dysfunction. Here, we investigate the role of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase system as a major component of reactive oxygen species generation, in a rodent model of early-onset preeclampsia induced by excess sFlt1 (soluble fms-like tyrosine kinase 1). Placenta and kidney samples were obtained from normal pregnant and PE rats to measure the sFlt1/PlGF (placental growth factor) ratio in addition to oxidative stress-related parameters, including the activities and expressions of NADPH oxidase isoforms (NOX1, NOX2, and NOX4), components of nitric oxide (NO) metabolism, and antioxidant enzymes. Peroxisome proliferator-activated receptors (PPARα, PPARγ) and cytokines IL1β, IL3, IL6, IL10, and IL18 were also measured to evaluate the inflammation status in our experimental setting. Excessive O2●− production was found in rats that were treated with sFlt1; interestingly, this alteration appears to be mediated mainly by NOX2 in the placenta and by NOX4 in the kidney. Altered NO metabolism and antioxidant defense systems, together with mitochondrial dysfunction, were observed in this model of PE. Preeclamptic animals also exhibited overexpression of proinflammatory biomarkers as well as increased collagen deposition. Our results highlight the role of NADPH oxidase in mediating oxidative stress and possibly inflammatory processes in the placenta and kidney of an sFlt1-based model of early-onset preeclampsia. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-01 2023-03-01T20:32:19Z 2023-03-01T20:32:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/antiox11081608 Antioxidants, v. 11, n. 8, 2022. 2076-3921 http://hdl.handle.net/11449/240774 10.3390/antiox11081608 2-s2.0-85137354426 |
url |
http://dx.doi.org/10.3390/antiox11081608 http://hdl.handle.net/11449/240774 |
identifier_str_mv |
Antioxidants, v. 11, n. 8, 2022. 2076-3921 10.3390/antiox11081608 2-s2.0-85137354426 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Antioxidants |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128927173967872 |