The influence of pre-treatment on germination of three species of Catasetum (Orchidaceae)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/2317-1545v45266264 http://hdl.handle.net/11449/249938 |
Resumo: | Asymbiotic germination techniques are successful in species of the Catasetum genus. The diverse composition of the culture media can be critical; however, viable seeds are required for asymbiotic germination. The reliable method for viability assessment is the tetrazolium test. A decisive step in the efficiency of the tetrazolium test is the seeds preconditioning with sucrose solution, which has the potential to improve germination. Therefore, this work aimed to evaluate the seed germination of three species of the genus Catasetum in culture media, preconditioned or not with sucrose solution. Seeds of the species Catasetum osculatum, Ctsm. galeritum and Ctsm. complanatum were assessed by tetrazolium test and germinated in two different media, pretreated or not with 10% sucrose solution. The statistical analysis showed that the use of sucrose pretreatment significantly increased the germination rates of the species. We conclude that the pretreatment with 10% sucrose for 24 hours, regardless of the species and the culture medium, significantly increases the germination of Ctsm. complanatum, Ctsm. galeritum and Ctsm. osculatum seeds. |
id |
UNSP_e48e46c8beb8fd1814c8389c74ce0c89 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/249938 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
The influence of pre-treatment on germination of three species of Catasetum (Orchidaceae)orchidplant cultureseedssucrose solutiontetrazoliumAsymbiotic germination techniques are successful in species of the Catasetum genus. The diverse composition of the culture media can be critical; however, viable seeds are required for asymbiotic germination. The reliable method for viability assessment is the tetrazolium test. A decisive step in the efficiency of the tetrazolium test is the seeds preconditioning with sucrose solution, which has the potential to improve germination. Therefore, this work aimed to evaluate the seed germination of three species of the genus Catasetum in culture media, preconditioned or not with sucrose solution. Seeds of the species Catasetum osculatum, Ctsm. galeritum and Ctsm. complanatum were assessed by tetrazolium test and germinated in two different media, pretreated or not with 10% sucrose solution. The statistical analysis showed that the use of sucrose pretreatment significantly increased the germination rates of the species. We conclude that the pretreatment with 10% sucrose for 24 hours, regardless of the species and the culture medium, significantly increases the germination of Ctsm. complanatum, Ctsm. galeritum and Ctsm. osculatum seeds.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Unoeste Campus II Agronomy College, Rod. Raposo Tavares Km 572, Bairro Limoeiro, SPUniversidade Estadual Paulista (Unesp) School of Sciences Humanities and Languages Department of Biotechnology, Avenida Dom Antonio, 2100, SPUniversidade Estadual Paulista (Unesp) School of Sciences Humanities and Languages Department of Biotechnology, Avenida Dom Antonio, 2100, SPCAPES: 001Agronomy CollegeUniversidade Estadual Paulista (UNESP)Moraes, Milena CristinaBertão, Monica Rosa [UNESP]Custódio, Ceci CastilhoMachado-Neto, Nelson Barbosa2023-07-29T16:13:17Z2023-07-29T16:13:17Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/2317-1545v45266264Journal of Seed Science, v. 45.2317-1537http://hdl.handle.net/11449/24993810.1590/2317-1545v452662642-s2.0-85158895860Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Seed Scienceinfo:eu-repo/semantics/openAccess2024-06-13T17:38:31Zoai:repositorio.unesp.br:11449/249938Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:59:39.216169Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The influence of pre-treatment on germination of three species of Catasetum (Orchidaceae) |
title |
The influence of pre-treatment on germination of three species of Catasetum (Orchidaceae) |
spellingShingle |
The influence of pre-treatment on germination of three species of Catasetum (Orchidaceae) Moraes, Milena Cristina orchid plant culture seeds sucrose solution tetrazolium |
title_short |
The influence of pre-treatment on germination of three species of Catasetum (Orchidaceae) |
title_full |
The influence of pre-treatment on germination of three species of Catasetum (Orchidaceae) |
title_fullStr |
The influence of pre-treatment on germination of three species of Catasetum (Orchidaceae) |
title_full_unstemmed |
The influence of pre-treatment on germination of three species of Catasetum (Orchidaceae) |
title_sort |
The influence of pre-treatment on germination of three species of Catasetum (Orchidaceae) |
author |
Moraes, Milena Cristina |
author_facet |
Moraes, Milena Cristina Bertão, Monica Rosa [UNESP] Custódio, Ceci Castilho Machado-Neto, Nelson Barbosa |
author_role |
author |
author2 |
Bertão, Monica Rosa [UNESP] Custódio, Ceci Castilho Machado-Neto, Nelson Barbosa |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Agronomy College Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Moraes, Milena Cristina Bertão, Monica Rosa [UNESP] Custódio, Ceci Castilho Machado-Neto, Nelson Barbosa |
dc.subject.por.fl_str_mv |
orchid plant culture seeds sucrose solution tetrazolium |
topic |
orchid plant culture seeds sucrose solution tetrazolium |
description |
Asymbiotic germination techniques are successful in species of the Catasetum genus. The diverse composition of the culture media can be critical; however, viable seeds are required for asymbiotic germination. The reliable method for viability assessment is the tetrazolium test. A decisive step in the efficiency of the tetrazolium test is the seeds preconditioning with sucrose solution, which has the potential to improve germination. Therefore, this work aimed to evaluate the seed germination of three species of the genus Catasetum in culture media, preconditioned or not with sucrose solution. Seeds of the species Catasetum osculatum, Ctsm. galeritum and Ctsm. complanatum were assessed by tetrazolium test and germinated in two different media, pretreated or not with 10% sucrose solution. The statistical analysis showed that the use of sucrose pretreatment significantly increased the germination rates of the species. We conclude that the pretreatment with 10% sucrose for 24 hours, regardless of the species and the culture medium, significantly increases the germination of Ctsm. complanatum, Ctsm. galeritum and Ctsm. osculatum seeds. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:13:17Z 2023-07-29T16:13:17Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/2317-1545v45266264 Journal of Seed Science, v. 45. 2317-1537 http://hdl.handle.net/11449/249938 10.1590/2317-1545v45266264 2-s2.0-85158895860 |
url |
http://dx.doi.org/10.1590/2317-1545v45266264 http://hdl.handle.net/11449/249938 |
identifier_str_mv |
Journal of Seed Science, v. 45. 2317-1537 10.1590/2317-1545v45266264 2-s2.0-85158895860 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Seed Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129009935974400 |