Efeito fotodinâmico da curcumina no tratamento da periodontite induzida em ratos: análise microbiológica

• Autor(a) principal: Ferreira, Renata Pironato [UNESP]
• Data de Publicação: 2014
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149586; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-27/000867188.pdf
• topic: Periodontite; Ratos; Fotoquimioterapia; Curcumina; Periodontitis

The objective of this study was to evaluate the viewpoint microbiological the influence of curcumin as a photosensitizing agent associated with irradiation Light Emitting Diode (LED), in antimicrobial photodynamic therapy (aPDT), for the treatment of experimentally induced periodontitis in rats. Ninety-six animals were subjected to induction of periodontitis installation via a cotton thread around the first molars, which was maintained for 7 days. Then the animals were randomly divided into 4 groups of 24 animals each, which received the following treatments, at 7, 15 and 30 days: G1 (n = 24) - represented by animals suffered no local treatment; G2 (n = 24) - irrigation with a solution of curcumin (40μM); G3 (n = 24) - irradiation with LED; G4 (n = 24) - irrigation with a solution of curcumin (40μM) followed by irradiation with LED. Local treatments were carried out 7 days after the induction of periodontitis. The LED (semiconductor Nitridio emitter of indium gallium) used in this study for the realization of aPDT, has a wavelength of 465 to 485nm, irradiance of 600 mW / cm ² and the treatment using the LED irradiation was performed for 60 seconds at buccal face of the first lower left molars. Treatment with the LED G4 was performed after 60 seconds of irrigation with the photosensitizer curcumin. Subgingival biofilm samples were collected using sterile absorbent paper cones, which were introduced into the gingival sulcus in apical direction until a slight resistance was felt, remaining in place for 30 seconds in the vestibular region of the left mandibular first molar at baseline, the post-treatment 7 days 15 and 30.The samples were processed by the technique of Polymerase Chain Reaction (PCR) researching the presence of Porphyromonas gingivalis (Pg) and Aggregatibacter actinomycetemcomitans (Aa).Was not detected at baseline...