Effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue: analysis by Raman spectroscopy, dual-energy X-ray absorptiometry, and mechanical resistance
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10103-019-02821-5 http://hdl.handle.net/11449/189348 |
Resumo: | The aim of the present study was to verify the effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue by Raman spectroscopy (Raman), dual-energy X-ray absorptiometry (DXA), and mechanical resistance (F-max) analysis. A total of 40 male Wistar animals, 60 days old, were used. The animals were distributed into four groups: control (C), control with GH (GHC), muscular strength training (T), and muscular strength training with GH (GHT). Blood samples were collected for the quantification of creatine kinase (CK-MB) and the femurs were removed for analysis by Raman, DXA, and F-max. A more pronounced increase in the bone mineral components was verified in the T group, for all the variables obtained by the Raman (calcium, phosphate, amide, and collagen). In addition, for animals submitted to GH supplementation, there was a reduction in the variable bone mineral density (BMD) obtained by the DXA (p < 0.05). Finally, the animals that received GH supplementation presented a higher F-max, but without statistical significance (p > 0.05). It was concluded that animals that received GH supplementation demonstrated a decrease in BMD. In addition, T alone was able to promote increased calcium, phosphate, amide, and collagen compounds in bone tissue. |
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Effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue: analysis by Raman spectroscopy, dual-energy X-ray absorptiometry, and mechanical resistanceAnabolicsBone tissueGrowth hormoneMuscular strengthPhysical trainingThe aim of the present study was to verify the effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue by Raman spectroscopy (Raman), dual-energy X-ray absorptiometry (DXA), and mechanical resistance (F-max) analysis. A total of 40 male Wistar animals, 60 days old, were used. The animals were distributed into four groups: control (C), control with GH (GHC), muscular strength training (T), and muscular strength training with GH (GHT). Blood samples were collected for the quantification of creatine kinase (CK-MB) and the femurs were removed for analysis by Raman, DXA, and F-max. A more pronounced increase in the bone mineral components was verified in the T group, for all the variables obtained by the Raman (calcium, phosphate, amide, and collagen). In addition, for animals submitted to GH supplementation, there was a reduction in the variable bone mineral density (BMD) obtained by the DXA (p < 0.05). Finally, the animals that received GH supplementation presented a higher F-max, but without statistical significance (p > 0.05). It was concluded that animals that received GH supplementation demonstrated a decrease in BMD. In addition, T alone was able to promote increased calcium, phosphate, amide, and collagen compounds in bone tissue.Faculty of Medical Sciences Universidade Estadual de Campinas– UNICAMP, Tessália Vieira de Camargo Street, 126. Neighbor: Cidade Universitária Zeferino Vaz.Department of Physical Education Universidade do Oeste Paulista – UNOESTE, Raposo Tavares Road, km 572 – Neighbor: LimoeiroLaboratório de Análise da Plasticidade Muscular – LAPMUS UNESP/FCT – Campus de Presidente Prudente, Rua Roberto Simonsen, 305, Bairro: Centro EducacionalFaculty of Veterinary Medicine Universidade do Oeste Paulista – UNOESTE, Raposo Tavares Road, km 572 – Neighbor: LimoeiroDepartment of Physical Therapy Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, campus de Presidente Prudente. Roberto Simonsen Street, 305. Neighbor: Centro EducacionalDepartment of Physics Chemistry and Biology Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, campus de Presidente Prudente. Roberto Simonsen Street, 305. Neighbor: Centro EducacionalDepartment of Animal Support Production and Health Universidade Estadual Paulista– UNESP, campus de Araçatuba. Clóvis Pestana Street, 793. Neighbor: IpanemaLaboratório de Análise da Plasticidade Muscular – LAPMUS UNESP/FCT – Campus de Presidente Prudente, Rua Roberto Simonsen, 305, Bairro: Centro EducacionalDepartment of Physical Therapy Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, campus de Presidente Prudente. Roberto Simonsen Street, 305. Neighbor: Centro EducacionalDepartment of Physics Chemistry and Biology Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, campus de Presidente Prudente. Roberto Simonsen Street, 305. Neighbor: Centro EducacionalDepartment of Animal Support Production and Health Universidade Estadual Paulista– UNESP, campus de Araçatuba. Clóvis Pestana Street, 793. Neighbor: IpanemaUniversidade Estadual de Campinas (UNICAMP)Universidade do Oeste Paulista – UNOESTEUniversidade Estadual Paulista (Unesp)Castoldi, Robson Chacon [UNESP]Ozaki, Guilherme Akio TamuraGarcia, Thiago AlvesGiometti, Ines CristinaKoike, Tatiana Emy [UNESP]Camargo, Regina Celi Trindade [UNESP]dos Santos Pereira, João Domingos Augusto [UNESP]Constantino, Carlos José Leopoldo [UNESP]Louzada, Mário Jefferson Quirino [UNESP]Camargo Filho, José Carlos Silva [UNESP]Belangero, William Dias2019-10-06T16:37:46Z2019-10-06T16:37:46Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10103-019-02821-5Lasers in Medical Science.1435-604X0268-8921http://hdl.handle.net/11449/18934810.1007/s10103-019-02821-52-s2.0-85068162315Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Scienceinfo:eu-repo/semantics/openAccess2021-10-23T08:46:54Zoai:repositorio.unesp.br:11449/189348Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T08:46:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue: analysis by Raman spectroscopy, dual-energy X-ray absorptiometry, and mechanical resistance |
title |
Effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue: analysis by Raman spectroscopy, dual-energy X-ray absorptiometry, and mechanical resistance |
spellingShingle |
Effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue: analysis by Raman spectroscopy, dual-energy X-ray absorptiometry, and mechanical resistance Castoldi, Robson Chacon [UNESP] Anabolics Bone tissue Growth hormone Muscular strength Physical training |
title_short |
Effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue: analysis by Raman spectroscopy, dual-energy X-ray absorptiometry, and mechanical resistance |
title_full |
Effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue: analysis by Raman spectroscopy, dual-energy X-ray absorptiometry, and mechanical resistance |
title_fullStr |
Effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue: analysis by Raman spectroscopy, dual-energy X-ray absorptiometry, and mechanical resistance |
title_full_unstemmed |
Effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue: analysis by Raman spectroscopy, dual-energy X-ray absorptiometry, and mechanical resistance |
title_sort |
Effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue: analysis by Raman spectroscopy, dual-energy X-ray absorptiometry, and mechanical resistance |
author |
Castoldi, Robson Chacon [UNESP] |
author_facet |
Castoldi, Robson Chacon [UNESP] Ozaki, Guilherme Akio Tamura Garcia, Thiago Alves Giometti, Ines Cristina Koike, Tatiana Emy [UNESP] Camargo, Regina Celi Trindade [UNESP] dos Santos Pereira, João Domingos Augusto [UNESP] Constantino, Carlos José Leopoldo [UNESP] Louzada, Mário Jefferson Quirino [UNESP] Camargo Filho, José Carlos Silva [UNESP] Belangero, William Dias |
author_role |
author |
author2 |
Ozaki, Guilherme Akio Tamura Garcia, Thiago Alves Giometti, Ines Cristina Koike, Tatiana Emy [UNESP] Camargo, Regina Celi Trindade [UNESP] dos Santos Pereira, João Domingos Augusto [UNESP] Constantino, Carlos José Leopoldo [UNESP] Louzada, Mário Jefferson Quirino [UNESP] Camargo Filho, José Carlos Silva [UNESP] Belangero, William Dias |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade do Oeste Paulista – UNOESTE Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Castoldi, Robson Chacon [UNESP] Ozaki, Guilherme Akio Tamura Garcia, Thiago Alves Giometti, Ines Cristina Koike, Tatiana Emy [UNESP] Camargo, Regina Celi Trindade [UNESP] dos Santos Pereira, João Domingos Augusto [UNESP] Constantino, Carlos José Leopoldo [UNESP] Louzada, Mário Jefferson Quirino [UNESP] Camargo Filho, José Carlos Silva [UNESP] Belangero, William Dias |
dc.subject.por.fl_str_mv |
Anabolics Bone tissue Growth hormone Muscular strength Physical training |
topic |
Anabolics Bone tissue Growth hormone Muscular strength Physical training |
description |
The aim of the present study was to verify the effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue by Raman spectroscopy (Raman), dual-energy X-ray absorptiometry (DXA), and mechanical resistance (F-max) analysis. A total of 40 male Wistar animals, 60 days old, were used. The animals were distributed into four groups: control (C), control with GH (GHC), muscular strength training (T), and muscular strength training with GH (GHT). Blood samples were collected for the quantification of creatine kinase (CK-MB) and the femurs were removed for analysis by Raman, DXA, and F-max. A more pronounced increase in the bone mineral components was verified in the T group, for all the variables obtained by the Raman (calcium, phosphate, amide, and collagen). In addition, for animals submitted to GH supplementation, there was a reduction in the variable bone mineral density (BMD) obtained by the DXA (p < 0.05). Finally, the animals that received GH supplementation presented a higher F-max, but without statistical significance (p > 0.05). It was concluded that animals that received GH supplementation demonstrated a decrease in BMD. In addition, T alone was able to promote increased calcium, phosphate, amide, and collagen compounds in bone tissue. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:37:46Z 2019-10-06T16:37:46Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10103-019-02821-5 Lasers in Medical Science. 1435-604X 0268-8921 http://hdl.handle.net/11449/189348 10.1007/s10103-019-02821-5 2-s2.0-85068162315 |
url |
http://dx.doi.org/10.1007/s10103-019-02821-5 http://hdl.handle.net/11449/189348 |
identifier_str_mv |
Lasers in Medical Science. 1435-604X 0268-8921 10.1007/s10103-019-02821-5 2-s2.0-85068162315 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers in Medical Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964571370258432 |