Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil

Detalhes bibliográficos
Autor(a) principal: Rocha, D. C.
Data de Publicação: 2009
Outros Autores: Rodella, R. A. [UNESP], Marino, C. l. [UNESP], Martins, D. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-83582009000300001
http://hdl.handle.net/11449/109936
Resumo: This work aims to carry out a comparative analysis using RAPD molecular markers in four Commelina weed species from the state of Paraná and C. benghalensis populations from the states of Paraná and São Paulo, Brazil. The genomic plant DNA sample was extracted from the leaves, separated, randomly fragmented and amplified by PCR. Random amplified polymorphic DNA fragments (RAPD markers) were analyzed by using POPGENE statistical program. Eighty-five primer sequences were tested but only three were suitable as molecular markers producing 37 DNA polymorphic fragments for comparisons among four Commelina species and 22 polymorphic fragments for comparisons among C. benghalensis populations. The results showed that there were inter-specific and intra-specific genetic variabilities among Commelina plant genera. Genetic diversity analysis between species indicated four mono-specific clusters and it was suggested to keep C. villosa as one species. Regarding the intra-specific genetic variability of C. benghalensis alone, three groups were verified, although there were 13 populations from two geographical areas. However, these clusters do not correspond to the distinct characteristics verified.
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spelling Genetic variability among Commelina weed species from the states of Paraná and São Paulo, BrazilVariabilidade genética entre espécies de plantas daninhas do gênero Commelina provenientes dos estados do Paraná e São Paulo, BrasilbiótipoCommelinaceaemarcadores molecularesplanta daninhabiotypeCommelinaceaemolecular markersweedsThis work aims to carry out a comparative analysis using RAPD molecular markers in four Commelina weed species from the state of Paraná and C. benghalensis populations from the states of Paraná and São Paulo, Brazil. The genomic plant DNA sample was extracted from the leaves, separated, randomly fragmented and amplified by PCR. Random amplified polymorphic DNA fragments (RAPD markers) were analyzed by using POPGENE statistical program. Eighty-five primer sequences were tested but only three were suitable as molecular markers producing 37 DNA polymorphic fragments for comparisons among four Commelina species and 22 polymorphic fragments for comparisons among C. benghalensis populations. The results showed that there were inter-specific and intra-specific genetic variabilities among Commelina plant genera. Genetic diversity analysis between species indicated four mono-specific clusters and it was suggested to keep C. villosa as one species. Regarding the intra-specific genetic variability of C. benghalensis alone, three groups were verified, although there were 13 populations from two geographical areas. However, these clusters do not correspond to the distinct characteristics verified.O presente trabalho procurou analisar a variabilidade genética, utilizando-se de marcadores RAPD, comparando quatro espécies de Commelina procedentes do Estado do Paraná e entre populações de C. benghalensis procedentes dos Estados do Paraná e São Paulo. Foi amostrado o DNA genômico extraído das folhas, o qual foi separado, fragmentado aleatoriamente e amplificado por PCR. Os fragmentos polimórficos de DNA (marcadores RAPD) foram analisados pelo programa estatístico POPGENE. Oitenta e cinco primers foram testados, mas somente três sequências foram consideradas marcadores moleculares, produzindo 37 bandas de DNA polimórfico para comparação entre as quatro espécies de Commelina e outras 22 para comparação entre as populações de C. benghalensis. Os resultados mostraram que há variabilidade genética inter e intraespecífica nas plantas do gênero Commelina. A análise da diversidade genética entre as espécies indicou quatro agrupamentos monoespecíficos, e isso sugere manter C. villosa como uma espécie. Considerando a variabilidade genética intraespecífica somente de C. benghalensis, apesar das 13 populações provenientes de dois Estados geográficos, três grupos foram identificados. Entretanto, esses agrupamentos não correspondem às características morfológicas distintas observadas.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual de Ponta Grossa Dep. de Biologia GeralUNESP Instituto de Biociências de Botucatu Dep. de BotânicaUNESP Instituto de Biociências de Botucatu Dep. de GenéticaUNESP Faculdade de Ciências Agronômicas Dep. de Produção VegetalUNESP Instituto de Biociências de Botucatu Dep. de BotânicaUNESP Instituto de Biociências de Botucatu Dep. de GenéticaUNESP Faculdade de Ciências Agronômicas Dep. de Produção VegetalSociedade Brasileira da Ciência das Plantas DaninhasUniversidade Estadual de Ponta Grossa (UEPG)Universidade Estadual Paulista (Unesp)Rocha, D. C.Rodella, R. A. [UNESP]Marino, C. l. [UNESP]Martins, D. [UNESP]2014-10-01T13:08:41Z2014-10-01T13:08:41Z2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article421-427application/pdfhttp://dx.doi.org/10.1590/S0100-83582009000300001Planta Daninha. Sociedade Brasileira da Ciência das Plantas Daninhas , v. 27, n. 3, p. 421-427, 2009.0100-8358http://hdl.handle.net/11449/10993610.1590/S0100-83582009000300001S0100-83582009000300001WOS:000270596800001S0100-83582009000300001.pdf142016625050040401653487382083190000-0003-4524-954XSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlanta Daninha0.5440,365info:eu-repo/semantics/openAccess2024-04-30T15:59:53Zoai:repositorio.unesp.br:11449/109936Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-30T15:59:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil
Variabilidade genética entre espécies de plantas daninhas do gênero Commelina provenientes dos estados do Paraná e São Paulo, Brasil
title Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil
spellingShingle Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil
Rocha, D. C.
biótipo
Commelinaceae
marcadores moleculares
planta daninha
biotype
Commelinaceae
molecular markers
weeds
title_short Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil
title_full Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil
title_fullStr Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil
title_full_unstemmed Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil
title_sort Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil
author Rocha, D. C.
author_facet Rocha, D. C.
Rodella, R. A. [UNESP]
Marino, C. l. [UNESP]
Martins, D. [UNESP]
author_role author
author2 Rodella, R. A. [UNESP]
Marino, C. l. [UNESP]
Martins, D. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Ponta Grossa (UEPG)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Rocha, D. C.
Rodella, R. A. [UNESP]
Marino, C. l. [UNESP]
Martins, D. [UNESP]
dc.subject.por.fl_str_mv biótipo
Commelinaceae
marcadores moleculares
planta daninha
biotype
Commelinaceae
molecular markers
weeds
topic biótipo
Commelinaceae
marcadores moleculares
planta daninha
biotype
Commelinaceae
molecular markers
weeds
description This work aims to carry out a comparative analysis using RAPD molecular markers in four Commelina weed species from the state of Paraná and C. benghalensis populations from the states of Paraná and São Paulo, Brazil. The genomic plant DNA sample was extracted from the leaves, separated, randomly fragmented and amplified by PCR. Random amplified polymorphic DNA fragments (RAPD markers) were analyzed by using POPGENE statistical program. Eighty-five primer sequences were tested but only three were suitable as molecular markers producing 37 DNA polymorphic fragments for comparisons among four Commelina species and 22 polymorphic fragments for comparisons among C. benghalensis populations. The results showed that there were inter-specific and intra-specific genetic variabilities among Commelina plant genera. Genetic diversity analysis between species indicated four mono-specific clusters and it was suggested to keep C. villosa as one species. Regarding the intra-specific genetic variability of C. benghalensis alone, three groups were verified, although there were 13 populations from two geographical areas. However, these clusters do not correspond to the distinct characteristics verified.
publishDate 2009
dc.date.none.fl_str_mv 2009
2014-10-01T13:08:41Z
2014-10-01T13:08:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-83582009000300001
Planta Daninha. Sociedade Brasileira da Ciência das Plantas Daninhas , v. 27, n. 3, p. 421-427, 2009.
0100-8358
http://hdl.handle.net/11449/109936
10.1590/S0100-83582009000300001
S0100-83582009000300001
WOS:000270596800001
S0100-83582009000300001.pdf
1420166250500404
0165348738208319
0000-0003-4524-954X
url http://dx.doi.org/10.1590/S0100-83582009000300001
http://hdl.handle.net/11449/109936
identifier_str_mv Planta Daninha. Sociedade Brasileira da Ciência das Plantas Daninhas , v. 27, n. 3, p. 421-427, 2009.
0100-8358
10.1590/S0100-83582009000300001
S0100-83582009000300001
WOS:000270596800001
S0100-83582009000300001.pdf
1420166250500404
0165348738208319
0000-0003-4524-954X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Planta Daninha
0.544
0,365
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 421-427
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira da Ciência das Plantas Daninhas
publisher.none.fl_str_mv Sociedade Brasileira da Ciência das Plantas Daninhas
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965666543927296

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