Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-83582009000300001 http://hdl.handle.net/11449/109936 |
Resumo: | This work aims to carry out a comparative analysis using RAPD molecular markers in four Commelina weed species from the state of Paraná and C. benghalensis populations from the states of Paraná and São Paulo, Brazil. The genomic plant DNA sample was extracted from the leaves, separated, randomly fragmented and amplified by PCR. Random amplified polymorphic DNA fragments (RAPD markers) were analyzed by using POPGENE statistical program. Eighty-five primer sequences were tested but only three were suitable as molecular markers producing 37 DNA polymorphic fragments for comparisons among four Commelina species and 22 polymorphic fragments for comparisons among C. benghalensis populations. The results showed that there were inter-specific and intra-specific genetic variabilities among Commelina plant genera. Genetic diversity analysis between species indicated four mono-specific clusters and it was suggested to keep C. villosa as one species. Regarding the intra-specific genetic variability of C. benghalensis alone, three groups were verified, although there were 13 populations from two geographical areas. However, these clusters do not correspond to the distinct characteristics verified. |
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Genetic variability among Commelina weed species from the states of Paraná and São Paulo, BrazilVariabilidade genética entre espécies de plantas daninhas do gênero Commelina provenientes dos estados do Paraná e São Paulo, BrasilbiótipoCommelinaceaemarcadores molecularesplanta daninhabiotypeCommelinaceaemolecular markersweedsThis work aims to carry out a comparative analysis using RAPD molecular markers in four Commelina weed species from the state of Paraná and C. benghalensis populations from the states of Paraná and São Paulo, Brazil. The genomic plant DNA sample was extracted from the leaves, separated, randomly fragmented and amplified by PCR. Random amplified polymorphic DNA fragments (RAPD markers) were analyzed by using POPGENE statistical program. Eighty-five primer sequences were tested but only three were suitable as molecular markers producing 37 DNA polymorphic fragments for comparisons among four Commelina species and 22 polymorphic fragments for comparisons among C. benghalensis populations. The results showed that there were inter-specific and intra-specific genetic variabilities among Commelina plant genera. Genetic diversity analysis between species indicated four mono-specific clusters and it was suggested to keep C. villosa as one species. Regarding the intra-specific genetic variability of C. benghalensis alone, three groups were verified, although there were 13 populations from two geographical areas. However, these clusters do not correspond to the distinct characteristics verified.O presente trabalho procurou analisar a variabilidade genética, utilizando-se de marcadores RAPD, comparando quatro espécies de Commelina procedentes do Estado do Paraná e entre populações de C. benghalensis procedentes dos Estados do Paraná e São Paulo. Foi amostrado o DNA genômico extraído das folhas, o qual foi separado, fragmentado aleatoriamente e amplificado por PCR. Os fragmentos polimórficos de DNA (marcadores RAPD) foram analisados pelo programa estatístico POPGENE. Oitenta e cinco primers foram testados, mas somente três sequências foram consideradas marcadores moleculares, produzindo 37 bandas de DNA polimórfico para comparação entre as quatro espécies de Commelina e outras 22 para comparação entre as populações de C. benghalensis. Os resultados mostraram que há variabilidade genética inter e intraespecífica nas plantas do gênero Commelina. A análise da diversidade genética entre as espécies indicou quatro agrupamentos monoespecíficos, e isso sugere manter C. villosa como uma espécie. Considerando a variabilidade genética intraespecífica somente de C. benghalensis, apesar das 13 populações provenientes de dois Estados geográficos, três grupos foram identificados. Entretanto, esses agrupamentos não correspondem às características morfológicas distintas observadas.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual de Ponta Grossa Dep. de Biologia GeralUNESP Instituto de Biociências de Botucatu Dep. de BotânicaUNESP Instituto de Biociências de Botucatu Dep. de GenéticaUNESP Faculdade de Ciências Agronômicas Dep. de Produção VegetalUNESP Instituto de Biociências de Botucatu Dep. de BotânicaUNESP Instituto de Biociências de Botucatu Dep. de GenéticaUNESP Faculdade de Ciências Agronômicas Dep. de Produção VegetalSociedade Brasileira da Ciência das Plantas DaninhasUniversidade Estadual de Ponta Grossa (UEPG)Universidade Estadual Paulista (Unesp)Rocha, D. C.Rodella, R. A. [UNESP]Marino, C. l. [UNESP]Martins, D. [UNESP]2014-10-01T13:08:41Z2014-10-01T13:08:41Z2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article421-427application/pdfhttp://dx.doi.org/10.1590/S0100-83582009000300001Planta Daninha. Sociedade Brasileira da Ciência das Plantas Daninhas , v. 27, n. 3, p. 421-427, 2009.0100-8358http://hdl.handle.net/11449/10993610.1590/S0100-83582009000300001S0100-83582009000300001WOS:000270596800001S0100-83582009000300001.pdf142016625050040401653487382083190000-0003-4524-954XSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlanta Daninha0.5440,365info:eu-repo/semantics/openAccess2024-04-30T15:59:53Zoai:repositorio.unesp.br:11449/109936Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:25:17.422620Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil Variabilidade genética entre espécies de plantas daninhas do gênero Commelina provenientes dos estados do Paraná e São Paulo, Brasil |
title |
Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil |
spellingShingle |
Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil Rocha, D. C. biótipo Commelinaceae marcadores moleculares planta daninha biotype Commelinaceae molecular markers weeds |
title_short |
Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil |
title_full |
Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil |
title_fullStr |
Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil |
title_full_unstemmed |
Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil |
title_sort |
Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil |
author |
Rocha, D. C. |
author_facet |
Rocha, D. C. Rodella, R. A. [UNESP] Marino, C. l. [UNESP] Martins, D. [UNESP] |
author_role |
author |
author2 |
Rodella, R. A. [UNESP] Marino, C. l. [UNESP] Martins, D. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Ponta Grossa (UEPG) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rocha, D. C. Rodella, R. A. [UNESP] Marino, C. l. [UNESP] Martins, D. [UNESP] |
dc.subject.por.fl_str_mv |
biótipo Commelinaceae marcadores moleculares planta daninha biotype Commelinaceae molecular markers weeds |
topic |
biótipo Commelinaceae marcadores moleculares planta daninha biotype Commelinaceae molecular markers weeds |
description |
This work aims to carry out a comparative analysis using RAPD molecular markers in four Commelina weed species from the state of Paraná and C. benghalensis populations from the states of Paraná and São Paulo, Brazil. The genomic plant DNA sample was extracted from the leaves, separated, randomly fragmented and amplified by PCR. Random amplified polymorphic DNA fragments (RAPD markers) were analyzed by using POPGENE statistical program. Eighty-five primer sequences were tested but only three were suitable as molecular markers producing 37 DNA polymorphic fragments for comparisons among four Commelina species and 22 polymorphic fragments for comparisons among C. benghalensis populations. The results showed that there were inter-specific and intra-specific genetic variabilities among Commelina plant genera. Genetic diversity analysis between species indicated four mono-specific clusters and it was suggested to keep C. villosa as one species. Regarding the intra-specific genetic variability of C. benghalensis alone, three groups were verified, although there were 13 populations from two geographical areas. However, these clusters do not correspond to the distinct characteristics verified. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2014-10-01T13:08:41Z 2014-10-01T13:08:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-83582009000300001 Planta Daninha. Sociedade Brasileira da Ciência das Plantas Daninhas , v. 27, n. 3, p. 421-427, 2009. 0100-8358 http://hdl.handle.net/11449/109936 10.1590/S0100-83582009000300001 S0100-83582009000300001 WOS:000270596800001 S0100-83582009000300001.pdf 1420166250500404 0165348738208319 0000-0003-4524-954X |
url |
http://dx.doi.org/10.1590/S0100-83582009000300001 http://hdl.handle.net/11449/109936 |
identifier_str_mv |
Planta Daninha. Sociedade Brasileira da Ciência das Plantas Daninhas , v. 27, n. 3, p. 421-427, 2009. 0100-8358 10.1590/S0100-83582009000300001 S0100-83582009000300001 WOS:000270596800001 S0100-83582009000300001.pdf 1420166250500404 0165348738208319 0000-0003-4524-954X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Planta Daninha 0.544 0,365 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
421-427 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira da Ciência das Plantas Daninhas |
publisher.none.fl_str_mv |
Sociedade Brasileira da Ciência das Plantas Daninhas |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129519228289024 |