Increased DSG2 plasmatic levels identified by transcriptomic-based secretome analysis is a potential prognostic biomarker in laryngeal carcinoma

Detalhes bibliográficos
Autor(a) principal: Cury, Sarah Santiloni [UNESP]
Data de Publicação: 2020
Outros Autores: Lapa, Rainer Marco Lopez [UNESP], de Mello, Julia Bette Homem, Marchi, Fábio Albuquerque, Domingues, Maria Aparecida Custódio [UNESP], Pinto, Clóvis Antonio Lopes, Carvalho, Robson Francisco [UNESP], de Carvalho, Genival Barbosa, Kowalski, Luiz Paulo, Rogatto, Silvia Regina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.oraloncology.2020.104592
http://hdl.handle.net/11449/201558
Resumo: Objectives: The tumor secretome deconvolution is a promising strategy to identify diagnostic and prognostic biomarkers. Here, transcriptomic-based secretome analysis was performed aiming to discover laryngeal squamous cell carcinomas (LSCC) biomarkers from potentially secreted proteins (PSPs). Material and Methods: The tumor expression profile (35 LSCC biopsies compared with surrounding normal tissues - SN) revealed 589 overexpressed genes. This gene list was used for secretome analysis based on laryngeal tumors and related secretome databases. Results: Forty-nine (Laryngeal tumor secretome database) and 50 (Human Protein Atlas and Cancer Secretome Database) PSPs presented an association with worse overall survival. Specifically, DSG2 overexpression was strongly correlated with poor survival and distant metastasis. DSG2 increased expression was confirmed in the LSCC dataset (LSCC = 111; SN = 12) from TCGA. A significant association between shorter survival and DSG2 overexpression was also detected. In an independent cohort of cases, we analyzed and confirmed high protein levels of DSG2 in plasma from LSCC patients. Conclusion: A set of PSPs including the circulating DSG2, were associated with shorter overall survival in LSCC. DSG2 overexpression was also correlated with distant metastasis. The high plasmatic protein levels of DSG2 suggest its potential to be tested in liquid biopsies and applied as prognostic biomarker of LSCC.
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spelling Increased DSG2 plasmatic levels identified by transcriptomic-based secretome analysis is a potential prognostic biomarker in laryngeal carcinomaBiomarkerLaryngeal squamous cell carcinomaLiquid biopsySecretomeTranscriptomeObjectives: The tumor secretome deconvolution is a promising strategy to identify diagnostic and prognostic biomarkers. Here, transcriptomic-based secretome analysis was performed aiming to discover laryngeal squamous cell carcinomas (LSCC) biomarkers from potentially secreted proteins (PSPs). Material and Methods: The tumor expression profile (35 LSCC biopsies compared with surrounding normal tissues - SN) revealed 589 overexpressed genes. This gene list was used for secretome analysis based on laryngeal tumors and related secretome databases. Results: Forty-nine (Laryngeal tumor secretome database) and 50 (Human Protein Atlas and Cancer Secretome Database) PSPs presented an association with worse overall survival. Specifically, DSG2 overexpression was strongly correlated with poor survival and distant metastasis. DSG2 increased expression was confirmed in the LSCC dataset (LSCC = 111; SN = 12) from TCGA. A significant association between shorter survival and DSG2 overexpression was also detected. In an independent cohort of cases, we analyzed and confirmed high protein levels of DSG2 in plasma from LSCC patients. Conclusion: A set of PSPs including the circulating DSG2, were associated with shorter overall survival in LSCC. DSG2 overexpression was also correlated with distant metastasis. The high plasmatic protein levels of DSG2 suggest its potential to be tested in liquid biopsies and applied as prognostic biomarker of LSCC.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Uddannelses- og ForskningsministerietDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)Department of Head and Neck Surgery and Otorhinolaryngology A.C. Camargo Cancer CenterInternational Research Center CIPE – A.C. Camargo Cancer CenterDepartment of Pathology Faculty of Medicine São Paulo State University (UNESP)Department of Pathology A.C. Camargo Cancer CenterDepartment of Clinical Genetics University Hospital Institute of Regional Health Research University of Southern DenmarkDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)Department of Pathology Faculty of Medicine São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)A.C. Camargo Cancer CenterCIPE – A.C. Camargo Cancer CenterUniversity of Southern DenmarkCury, Sarah Santiloni [UNESP]Lapa, Rainer Marco Lopez [UNESP]de Mello, Julia Bette HomemMarchi, Fábio AlbuquerqueDomingues, Maria Aparecida Custódio [UNESP]Pinto, Clóvis Antonio LopesCarvalho, Robson Francisco [UNESP]de Carvalho, Genival BarbosaKowalski, Luiz PauloRogatto, Silvia Regina2020-12-12T02:35:43Z2020-12-12T02:35:43Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.oraloncology.2020.104592Oral Oncology, v. 103.1879-05931368-8375http://hdl.handle.net/11449/20155810.1016/j.oraloncology.2020.1045922-s2.0-85079538957Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOral Oncologyinfo:eu-repo/semantics/openAccess2021-10-22T20:18:56Zoai:repositorio.unesp.br:11449/201558Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:21:38.861093Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Increased DSG2 plasmatic levels identified by transcriptomic-based secretome analysis is a potential prognostic biomarker in laryngeal carcinoma
title Increased DSG2 plasmatic levels identified by transcriptomic-based secretome analysis is a potential prognostic biomarker in laryngeal carcinoma
spellingShingle Increased DSG2 plasmatic levels identified by transcriptomic-based secretome analysis is a potential prognostic biomarker in laryngeal carcinoma
Cury, Sarah Santiloni [UNESP]
Biomarker
Laryngeal squamous cell carcinoma
Liquid biopsy
Secretome
Transcriptome
title_short Increased DSG2 plasmatic levels identified by transcriptomic-based secretome analysis is a potential prognostic biomarker in laryngeal carcinoma
title_full Increased DSG2 plasmatic levels identified by transcriptomic-based secretome analysis is a potential prognostic biomarker in laryngeal carcinoma
title_fullStr Increased DSG2 plasmatic levels identified by transcriptomic-based secretome analysis is a potential prognostic biomarker in laryngeal carcinoma
title_full_unstemmed Increased DSG2 plasmatic levels identified by transcriptomic-based secretome analysis is a potential prognostic biomarker in laryngeal carcinoma
title_sort Increased DSG2 plasmatic levels identified by transcriptomic-based secretome analysis is a potential prognostic biomarker in laryngeal carcinoma
author Cury, Sarah Santiloni [UNESP]
author_facet Cury, Sarah Santiloni [UNESP]
Lapa, Rainer Marco Lopez [UNESP]
de Mello, Julia Bette Homem
Marchi, Fábio Albuquerque
Domingues, Maria Aparecida Custódio [UNESP]
Pinto, Clóvis Antonio Lopes
Carvalho, Robson Francisco [UNESP]
de Carvalho, Genival Barbosa
Kowalski, Luiz Paulo
Rogatto, Silvia Regina
author_role author
author2 Lapa, Rainer Marco Lopez [UNESP]
de Mello, Julia Bette Homem
Marchi, Fábio Albuquerque
Domingues, Maria Aparecida Custódio [UNESP]
Pinto, Clóvis Antonio Lopes
Carvalho, Robson Francisco [UNESP]
de Carvalho, Genival Barbosa
Kowalski, Luiz Paulo
Rogatto, Silvia Regina
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
A.C. Camargo Cancer Center
CIPE – A.C. Camargo Cancer Center
University of Southern Denmark
dc.contributor.author.fl_str_mv Cury, Sarah Santiloni [UNESP]
Lapa, Rainer Marco Lopez [UNESP]
de Mello, Julia Bette Homem
Marchi, Fábio Albuquerque
Domingues, Maria Aparecida Custódio [UNESP]
Pinto, Clóvis Antonio Lopes
Carvalho, Robson Francisco [UNESP]
de Carvalho, Genival Barbosa
Kowalski, Luiz Paulo
Rogatto, Silvia Regina
dc.subject.por.fl_str_mv Biomarker
Laryngeal squamous cell carcinoma
Liquid biopsy
Secretome
Transcriptome
topic Biomarker
Laryngeal squamous cell carcinoma
Liquid biopsy
Secretome
Transcriptome
description Objectives: The tumor secretome deconvolution is a promising strategy to identify diagnostic and prognostic biomarkers. Here, transcriptomic-based secretome analysis was performed aiming to discover laryngeal squamous cell carcinomas (LSCC) biomarkers from potentially secreted proteins (PSPs). Material and Methods: The tumor expression profile (35 LSCC biopsies compared with surrounding normal tissues - SN) revealed 589 overexpressed genes. This gene list was used for secretome analysis based on laryngeal tumors and related secretome databases. Results: Forty-nine (Laryngeal tumor secretome database) and 50 (Human Protein Atlas and Cancer Secretome Database) PSPs presented an association with worse overall survival. Specifically, DSG2 overexpression was strongly correlated with poor survival and distant metastasis. DSG2 increased expression was confirmed in the LSCC dataset (LSCC = 111; SN = 12) from TCGA. A significant association between shorter survival and DSG2 overexpression was also detected. In an independent cohort of cases, we analyzed and confirmed high protein levels of DSG2 in plasma from LSCC patients. Conclusion: A set of PSPs including the circulating DSG2, were associated with shorter overall survival in LSCC. DSG2 overexpression was also correlated with distant metastasis. The high plasmatic protein levels of DSG2 suggest its potential to be tested in liquid biopsies and applied as prognostic biomarker of LSCC.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:35:43Z
2020-12-12T02:35:43Z
2020-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.oraloncology.2020.104592
Oral Oncology, v. 103.
1879-0593
1368-8375
http://hdl.handle.net/11449/201558
10.1016/j.oraloncology.2020.104592
2-s2.0-85079538957
url http://dx.doi.org/10.1016/j.oraloncology.2020.104592
http://hdl.handle.net/11449/201558
identifier_str_mv Oral Oncology, v. 103.
1879-0593
1368-8375
10.1016/j.oraloncology.2020.104592
2-s2.0-85079538957
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Oral Oncology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128924529459200