The effects of increased heme oxygenase-1 on the lymphoproliferative response in dogs with visceral leishmaniasis

• Autor(a) principal: Almeida, Breno Fernando Martins de [UNESP]
• Data de Publicação: 2017
• Outros Autores: Silva, Kathlenn Liezbeth Oliveira [UNESP], Chiku, Vanessa Marim [UNESP], Leal, Aline Aparecida Correa [UNESP], Venturin, Gabriela Lovizutto [UNESP], Narciso, Luis Gustavo [UNESP], Fink, Maria Fernanda Cereijido Bersni [UNESP], Eugênio, Flavia de Rezende [UNESP], Santos, Paulo Sergio Patto dos [UNESP], Ciarlini, Paulo Cesar [UNESP], Lima, Valéria Marçal Felix de [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.imbio.2016.12.006; http://hdl.handle.net/11449/174042
• topic: Canine; HSP32; Leishmania infantum; Lymphoproliferation

Canine visceral leishmaniasis (CVL) is known to affect the cellular immunity of infected dogs, through impairing lymphoproliferation and microbicidal mechanisms. This study examined heme oxygenase-1 (HO-1) and its metabolites, oxidative stress and IL-10 levels in CVL and investigated correlations between these parameters. Additionally, the effects of HO-1 inhibition on the lymphoproliferative response and cytokine production in lymph node cells (LNCs) from infected dogs were evaluated. Forty-four dogs, 24 controls and 20 dogs with CVL were selected. Plasma and splenic levels of HO-1, haptoglobin, soluble CD163 receptor, ferritin and IL-10 were determined using capture ELISA. The HO-1 levels and relative gene expression in peripheral blood and bone marrow mononuclear cells were also determined. LNCs proliferation was evaluated with an HO-1 activator and with an HO-1 inhibitor, in the presence of the Leishmania infantum soluble antigen (SAgL), using flow cytometry. HO-1, IL-2, IFN-gamma and IL-10 were also determined in these cultures using capture ELISA. Infected dogs presented oxidative stress and increased HO-1 levels and relative gene expression, with correlation between oxidative stress and HO-1. The substances from heme metabolism and IL-10 were also elevated in the plasma and spleens of infected dogs. IL-10 and HO-1 levels were positively correlated with one another. Inhibition of HO-1 increased LNCs proliferation and decreased IL-10 and IL-2 production in the presence of SAgL. The increased HO-1 metabolism observed in CVL is probably associated with oxidative stress and increased IL-10, which could be one of the mechanisms responsible for inhibition of the lymphoproliferative response in sick dogs.