Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA.
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2334/josnusd.49.213 http://hdl.handle.net/11449/69857 |
Resumo: | A study was conducted to evaluate in vitro the effect of root surface conditioning with basic fibroblast growth factor (b-FGF) on morphology and proliferation of fibroblasts. Three experimental groups were used: non-treated, and treated with 50 microg or 125 microg b-FGF/ml. The dentin samples in each group were divided into subgroups according to the chemical treatment received before application of b-FGF: none, or conditioned with tetracycline-HCl or EDTA. After contact with b-FGF for 5 min, the samples were incubated for 24 h with 1 ml of culture medium containing 1 x 10(5) cells/ml plus 1 ml of culture medium alone. The samples were then subjected to routine preparation for SEM, and random fields were photographed. Three calibrated and blind examiners performed the assessment of morphology and density according to two index systems. Classification and regression trees indicated that the root surfaces treated with 125 microg b-FGF and previously conditioned with tetracycline-HCl or EDTA presented a morphology more suggestive of cellular adhesion and viability (P = 0.004). The density of fibroblasts on samples previously conditioned with EDTA, regardless of treatment with b-FGF, was significantly higher than in the other groups (P < 0.001). The present findings suggest that topical application of b-FGF has a positive influence on both the density and morphology of fibroblasts. |
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Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA.chelating agentedetic acidfibroblast growth factor 2protein synthesis inhibitortetracyclinecell adhesioncell culturecell proliferationcell shapecytologydentindrug effectfibroblasthumanpathologyperiodontitisregression analysistooth rootCell AdhesionCell ProliferationCell ShapeCells, CulturedChelating AgentsDentinEdetic AcidFibroblast Growth Factor 2FibroblastsHumansPeriodontitisProtein Synthesis InhibitorsRegression AnalysisTetracyclineTooth RootA study was conducted to evaluate in vitro the effect of root surface conditioning with basic fibroblast growth factor (b-FGF) on morphology and proliferation of fibroblasts. Three experimental groups were used: non-treated, and treated with 50 microg or 125 microg b-FGF/ml. The dentin samples in each group were divided into subgroups according to the chemical treatment received before application of b-FGF: none, or conditioned with tetracycline-HCl or EDTA. After contact with b-FGF for 5 min, the samples were incubated for 24 h with 1 ml of culture medium containing 1 x 10(5) cells/ml plus 1 ml of culture medium alone. The samples were then subjected to routine preparation for SEM, and random fields were photographed. Three calibrated and blind examiners performed the assessment of morphology and density according to two index systems. Classification and regression trees indicated that the root surfaces treated with 125 microg b-FGF and previously conditioned with tetracycline-HCl or EDTA presented a morphology more suggestive of cellular adhesion and viability (P = 0.004). The density of fibroblasts on samples previously conditioned with EDTA, regardless of treatment with b-FGF, was significantly higher than in the other groups (P < 0.001). The present findings suggest that topical application of b-FGF has a positive influence on both the density and morphology of fibroblasts.Universidade Estadual Paulista (Unesp)Silvério, Karina GMartinez, Aurora E TRossa Júnior, Carlos [UNESP]2014-05-27T11:22:34Z2014-05-27T11:22:34Z2007-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article213-220application/pdfhttp://dx.doi.org/10.2334/josnusd.49.213Journal of oral science, v. 49, n. 3, p. 213-220, 2007.1343-4934http://hdl.handle.net/11449/6985710.2334/josnusd.49.2132-s2.0-414491007022-s2.0-41449100702.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of oral science0.8530,397info:eu-repo/semantics/openAccess2023-11-27T06:09:55Zoai:repositorio.unesp.br:11449/69857Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:49:33.241193Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA. |
title |
Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA. |
spellingShingle |
Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA. Silvério, Karina G chelating agent edetic acid fibroblast growth factor 2 protein synthesis inhibitor tetracycline cell adhesion cell culture cell proliferation cell shape cytology dentin drug effect fibroblast human pathology periodontitis regression analysis tooth root Cell Adhesion Cell Proliferation Cell Shape Cells, Cultured Chelating Agents Dentin Edetic Acid Fibroblast Growth Factor 2 Fibroblasts Humans Periodontitis Protein Synthesis Inhibitors Regression Analysis Tetracycline Tooth Root |
title_short |
Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA. |
title_full |
Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA. |
title_fullStr |
Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA. |
title_full_unstemmed |
Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA. |
title_sort |
Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA. |
author |
Silvério, Karina G |
author_facet |
Silvério, Karina G Martinez, Aurora E T Rossa Júnior, Carlos [UNESP] |
author_role |
author |
author2 |
Martinez, Aurora E T Rossa Júnior, Carlos [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silvério, Karina G Martinez, Aurora E T Rossa Júnior, Carlos [UNESP] |
dc.subject.por.fl_str_mv |
chelating agent edetic acid fibroblast growth factor 2 protein synthesis inhibitor tetracycline cell adhesion cell culture cell proliferation cell shape cytology dentin drug effect fibroblast human pathology periodontitis regression analysis tooth root Cell Adhesion Cell Proliferation Cell Shape Cells, Cultured Chelating Agents Dentin Edetic Acid Fibroblast Growth Factor 2 Fibroblasts Humans Periodontitis Protein Synthesis Inhibitors Regression Analysis Tetracycline Tooth Root |
topic |
chelating agent edetic acid fibroblast growth factor 2 protein synthesis inhibitor tetracycline cell adhesion cell culture cell proliferation cell shape cytology dentin drug effect fibroblast human pathology periodontitis regression analysis tooth root Cell Adhesion Cell Proliferation Cell Shape Cells, Cultured Chelating Agents Dentin Edetic Acid Fibroblast Growth Factor 2 Fibroblasts Humans Periodontitis Protein Synthesis Inhibitors Regression Analysis Tetracycline Tooth Root |
description |
A study was conducted to evaluate in vitro the effect of root surface conditioning with basic fibroblast growth factor (b-FGF) on morphology and proliferation of fibroblasts. Three experimental groups were used: non-treated, and treated with 50 microg or 125 microg b-FGF/ml. The dentin samples in each group were divided into subgroups according to the chemical treatment received before application of b-FGF: none, or conditioned with tetracycline-HCl or EDTA. After contact with b-FGF for 5 min, the samples were incubated for 24 h with 1 ml of culture medium containing 1 x 10(5) cells/ml plus 1 ml of culture medium alone. The samples were then subjected to routine preparation for SEM, and random fields were photographed. Three calibrated and blind examiners performed the assessment of morphology and density according to two index systems. Classification and regression trees indicated that the root surfaces treated with 125 microg b-FGF and previously conditioned with tetracycline-HCl or EDTA presented a morphology more suggestive of cellular adhesion and viability (P = 0.004). The density of fibroblasts on samples previously conditioned with EDTA, regardless of treatment with b-FGF, was significantly higher than in the other groups (P < 0.001). The present findings suggest that topical application of b-FGF has a positive influence on both the density and morphology of fibroblasts. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-09-01 2014-05-27T11:22:34Z 2014-05-27T11:22:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2334/josnusd.49.213 Journal of oral science, v. 49, n. 3, p. 213-220, 2007. 1343-4934 http://hdl.handle.net/11449/69857 10.2334/josnusd.49.213 2-s2.0-41449100702 2-s2.0-41449100702.pdf |
url |
http://dx.doi.org/10.2334/josnusd.49.213 http://hdl.handle.net/11449/69857 |
identifier_str_mv |
Journal of oral science, v. 49, n. 3, p. 213-220, 2007. 1343-4934 10.2334/josnusd.49.213 2-s2.0-41449100702 2-s2.0-41449100702.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of oral science 0.853 0,397 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
213-220 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128985647808513 |