Biocompatibilidade e capacidade de mineralização de um cimento biocerâmico experimental: estudo em tecido subcutâneo de ratos

• Autor(a) principal: Oliveira, Pedro Henrique Chaves de [UNESP]
• Data de Publicação: 2017
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/156747; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2018-06-12/000901968.pdf
• topic: Teste de materiais; Cimentos dentários; Ratos; Materials testing

In order to develop a bioceramic experimental cement (BIOEXP), the biosealer, which contains calcium hydroxide in its formulation, the proposal was to evaluate its tissue response, comparing it to the Fillapex MTA and the AH Plus. It was used 64 polyethylene tubes and 16 rats (4 tubes per mouse) divided into 4 groups: G1 - control (empty tubes); G2 - tubes containing BIOEXP; G3 - tubes containing the MTA Fillapex cement, G4 - tubes containing the AH Plus. After 7 and 30 days, the animals were sacrificed and the tubes removed and fixed in 10% formalin solution. The specimens were processed for light microscopy analysis, with staining of H.E. and Von Kossa, and no staining for polarized light. Tissue sections were assessed by scores and the data were analyzed by the Kruskall Wallis and Dunn tests, with a significance level of 5% (p <0.05). The tissue response was evaluated according to the norms for tissue biocompatibility tests (ISO 10993-6: 2007). At 7 days, the cements presented a moderate to severe inflammatory response, which reduced to mild to moderate at 30 days. The experimental Bioceramic cement was biocompatible and with biomineralization capacity similar to the MTA Fillapex