Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar)

Detalhes bibliográficos
Autor(a) principal: Moreira, Stella H. [UNESP]
Data de Publicação: 2020
Outros Autores: Pazzini, Josiane M., Alvarez, Jorge L. G. [UNESP], Cassino, Pedro C. [UNESP], Bustamante, Caio C. [UNESP], Bernardes, Filippo J. L. [UNESP], Kajiura, Caroline Y. [UNESP], De Nardi, Andrigo B. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10103-019-02917-y
http://hdl.handle.net/11449/196619
Resumo: The reconstructive techniques have been widely used in Veterinary Medicine. The post-operative adjuvants therapies like the low-level laser therapy (LLLT) are used to decrease inherent complications to reconstructive surgeries. This article purposed to define the LLLT effects on the healing, inflammation, and vascularization of the skin grafts in applicable time intervals to veterinary surgical routine. Forty rats (Rattus norvegicus albinus wistar) were used and each one was submitted to autogenous cutaneous mesh grafting in the interescapular region. The rats were randomly distributed in five groups (G1, G2, G3, G4, and G5) in accordance with the 6 J/cm(2) or 10 J/cm(2) dose every 3 or 5 days. These treatments were applied on the skin graft for 15 days. The histochemical evaluation with Picrosirius showed greater expression of collagen type 1 - red in grafts of G5 (p < 0.05), while in G1 did not; the expression of collagen type III - green was not induced by LLLT. The histochemical evaluation with hematoxylin-eosin showed greater numbers of fibroblasts in grafts of G4 (p < 0.05) and less hemorrhage in grafts of G5 (p < 0.05). There was modulation of the inflammatory response in irradiated skin grafts. It is concluded the exhibition of the skin grafts to 6 J/cm(2) or 10 J/cm(2) dose every 5 days improved the healing and the modulation of the local inflammation.
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spelling Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar)PicrosiriusLow-level laser therapySkin graftCOX-2CD31HistologyThe reconstructive techniques have been widely used in Veterinary Medicine. The post-operative adjuvants therapies like the low-level laser therapy (LLLT) are used to decrease inherent complications to reconstructive surgeries. This article purposed to define the LLLT effects on the healing, inflammation, and vascularization of the skin grafts in applicable time intervals to veterinary surgical routine. Forty rats (Rattus norvegicus albinus wistar) were used and each one was submitted to autogenous cutaneous mesh grafting in the interescapular region. The rats were randomly distributed in five groups (G1, G2, G3, G4, and G5) in accordance with the 6 J/cm(2) or 10 J/cm(2) dose every 3 or 5 days. These treatments were applied on the skin graft for 15 days. The histochemical evaluation with Picrosirius showed greater expression of collagen type 1 - red in grafts of G5 (p < 0.05), while in G1 did not; the expression of collagen type III - green was not induced by LLLT. The histochemical evaluation with hematoxylin-eosin showed greater numbers of fibroblasts in grafts of G4 (p < 0.05) and less hemorrhage in grafts of G5 (p < 0.05). There was modulation of the inflammatory response in irradiated skin grafts. It is concluded the exhibition of the skin grafts to 6 J/cm(2) or 10 J/cm(2) dose every 5 days improved the healing and the modulation of the local inflammation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Clin & Cirurgia Vet, Jaboticabal, SP, BrazilUniv Estadual Paulista, Dept Clin & Cirurgia Vet, Fac Ciencias Agr & Vet, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilHosp Canc Barretos, Ctr Pesquisa Oncol Mol, Barretos, SP, BrazilUniv Estadual Paulista, Dept Clin & Cirurgia Vet, Jaboticabal, SP, BrazilUniv Estadual Paulista, Dept Clin & Cirurgia Vet, Fac Ciencias Agr & Vet, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2017/25951-9SpringerUniversidade Estadual Paulista (Unesp)Hosp Canc BarretosMoreira, Stella H. [UNESP]Pazzini, Josiane M.Alvarez, Jorge L. G. [UNESP]Cassino, Pedro C. [UNESP]Bustamante, Caio C. [UNESP]Bernardes, Filippo J. L. [UNESP]Kajiura, Caroline Y. [UNESP]De Nardi, Andrigo B. [UNESP]2020-12-10T19:50:40Z2020-12-10T19:50:40Z2020-02-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1103-1109http://dx.doi.org/10.1007/s10103-019-02917-yLasers In Medical Science. London: Springer London Ltd, v. 35, n. 5, p. 1103-1109, 2020.0268-8921http://hdl.handle.net/11449/19661910.1007/s10103-019-02917-yWOS:000516151100001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers In Medical Scienceinfo:eu-repo/semantics/openAccess2024-06-06T14:10:09Zoai:repositorio.unesp.br:11449/196619Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:00:37.843346Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar)
title Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar)
spellingShingle Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar)
Moreira, Stella H. [UNESP]
Picrosirius
Low-level laser therapy
Skin graft
COX-2
CD31
Histology
title_short Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar)
title_full Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar)
title_fullStr Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar)
title_full_unstemmed Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar)
title_sort Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar)
author Moreira, Stella H. [UNESP]
author_facet Moreira, Stella H. [UNESP]
Pazzini, Josiane M.
Alvarez, Jorge L. G. [UNESP]
Cassino, Pedro C. [UNESP]
Bustamante, Caio C. [UNESP]
Bernardes, Filippo J. L. [UNESP]
Kajiura, Caroline Y. [UNESP]
De Nardi, Andrigo B. [UNESP]
author_role author
author2 Pazzini, Josiane M.
Alvarez, Jorge L. G. [UNESP]
Cassino, Pedro C. [UNESP]
Bustamante, Caio C. [UNESP]
Bernardes, Filippo J. L. [UNESP]
Kajiura, Caroline Y. [UNESP]
De Nardi, Andrigo B. [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Hosp Canc Barretos
dc.contributor.author.fl_str_mv Moreira, Stella H. [UNESP]
Pazzini, Josiane M.
Alvarez, Jorge L. G. [UNESP]
Cassino, Pedro C. [UNESP]
Bustamante, Caio C. [UNESP]
Bernardes, Filippo J. L. [UNESP]
Kajiura, Caroline Y. [UNESP]
De Nardi, Andrigo B. [UNESP]
dc.subject.por.fl_str_mv Picrosirius
Low-level laser therapy
Skin graft
COX-2
CD31
Histology
topic Picrosirius
Low-level laser therapy
Skin graft
COX-2
CD31
Histology
description The reconstructive techniques have been widely used in Veterinary Medicine. The post-operative adjuvants therapies like the low-level laser therapy (LLLT) are used to decrease inherent complications to reconstructive surgeries. This article purposed to define the LLLT effects on the healing, inflammation, and vascularization of the skin grafts in applicable time intervals to veterinary surgical routine. Forty rats (Rattus norvegicus albinus wistar) were used and each one was submitted to autogenous cutaneous mesh grafting in the interescapular region. The rats were randomly distributed in five groups (G1, G2, G3, G4, and G5) in accordance with the 6 J/cm(2) or 10 J/cm(2) dose every 3 or 5 days. These treatments were applied on the skin graft for 15 days. The histochemical evaluation with Picrosirius showed greater expression of collagen type 1 - red in grafts of G5 (p < 0.05), while in G1 did not; the expression of collagen type III - green was not induced by LLLT. The histochemical evaluation with hematoxylin-eosin showed greater numbers of fibroblasts in grafts of G4 (p < 0.05) and less hemorrhage in grafts of G5 (p < 0.05). There was modulation of the inflammatory response in irradiated skin grafts. It is concluded the exhibition of the skin grafts to 6 J/cm(2) or 10 J/cm(2) dose every 5 days improved the healing and the modulation of the local inflammation.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-10T19:50:40Z
2020-12-10T19:50:40Z
2020-02-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10103-019-02917-y
Lasers In Medical Science. London: Springer London Ltd, v. 35, n. 5, p. 1103-1109, 2020.
0268-8921
http://hdl.handle.net/11449/196619
10.1007/s10103-019-02917-y
WOS:000516151100001
url http://dx.doi.org/10.1007/s10103-019-02917-y
http://hdl.handle.net/11449/196619
identifier_str_mv Lasers In Medical Science. London: Springer London Ltd, v. 35, n. 5, p. 1103-1109, 2020.
0268-8921
10.1007/s10103-019-02917-y
WOS:000516151100001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lasers In Medical Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1103-1109
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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