Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10103-019-02917-y http://hdl.handle.net/11449/196619 |
Resumo: | The reconstructive techniques have been widely used in Veterinary Medicine. The post-operative adjuvants therapies like the low-level laser therapy (LLLT) are used to decrease inherent complications to reconstructive surgeries. This article purposed to define the LLLT effects on the healing, inflammation, and vascularization of the skin grafts in applicable time intervals to veterinary surgical routine. Forty rats (Rattus norvegicus albinus wistar) were used and each one was submitted to autogenous cutaneous mesh grafting in the interescapular region. The rats were randomly distributed in five groups (G1, G2, G3, G4, and G5) in accordance with the 6 J/cm(2) or 10 J/cm(2) dose every 3 or 5 days. These treatments were applied on the skin graft for 15 days. The histochemical evaluation with Picrosirius showed greater expression of collagen type 1 - red in grafts of G5 (p < 0.05), while in G1 did not; the expression of collagen type III - green was not induced by LLLT. The histochemical evaluation with hematoxylin-eosin showed greater numbers of fibroblasts in grafts of G4 (p < 0.05) and less hemorrhage in grafts of G5 (p < 0.05). There was modulation of the inflammatory response in irradiated skin grafts. It is concluded the exhibition of the skin grafts to 6 J/cm(2) or 10 J/cm(2) dose every 5 days improved the healing and the modulation of the local inflammation. |
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Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar)PicrosiriusLow-level laser therapySkin graftCOX-2CD31HistologyThe reconstructive techniques have been widely used in Veterinary Medicine. The post-operative adjuvants therapies like the low-level laser therapy (LLLT) are used to decrease inherent complications to reconstructive surgeries. This article purposed to define the LLLT effects on the healing, inflammation, and vascularization of the skin grafts in applicable time intervals to veterinary surgical routine. Forty rats (Rattus norvegicus albinus wistar) were used and each one was submitted to autogenous cutaneous mesh grafting in the interescapular region. The rats were randomly distributed in five groups (G1, G2, G3, G4, and G5) in accordance with the 6 J/cm(2) or 10 J/cm(2) dose every 3 or 5 days. These treatments were applied on the skin graft for 15 days. The histochemical evaluation with Picrosirius showed greater expression of collagen type 1 - red in grafts of G5 (p < 0.05), while in G1 did not; the expression of collagen type III - green was not induced by LLLT. The histochemical evaluation with hematoxylin-eosin showed greater numbers of fibroblasts in grafts of G4 (p < 0.05) and less hemorrhage in grafts of G5 (p < 0.05). There was modulation of the inflammatory response in irradiated skin grafts. It is concluded the exhibition of the skin grafts to 6 J/cm(2) or 10 J/cm(2) dose every 5 days improved the healing and the modulation of the local inflammation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Clin & Cirurgia Vet, Jaboticabal, SP, BrazilUniv Estadual Paulista, Dept Clin & Cirurgia Vet, Fac Ciencias Agr & Vet, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilHosp Canc Barretos, Ctr Pesquisa Oncol Mol, Barretos, SP, BrazilUniv Estadual Paulista, Dept Clin & Cirurgia Vet, Jaboticabal, SP, BrazilUniv Estadual Paulista, Dept Clin & Cirurgia Vet, Fac Ciencias Agr & Vet, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2017/25951-9SpringerUniversidade Estadual Paulista (Unesp)Hosp Canc BarretosMoreira, Stella H. [UNESP]Pazzini, Josiane M.Alvarez, Jorge L. G. [UNESP]Cassino, Pedro C. [UNESP]Bustamante, Caio C. [UNESP]Bernardes, Filippo J. L. [UNESP]Kajiura, Caroline Y. [UNESP]De Nardi, Andrigo B. [UNESP]2020-12-10T19:50:40Z2020-12-10T19:50:40Z2020-02-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1103-1109http://dx.doi.org/10.1007/s10103-019-02917-yLasers In Medical Science. London: Springer London Ltd, v. 35, n. 5, p. 1103-1109, 2020.0268-8921http://hdl.handle.net/11449/19661910.1007/s10103-019-02917-yWOS:000516151100001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers In Medical Scienceinfo:eu-repo/semantics/openAccess2024-06-06T14:10:09Zoai:repositorio.unesp.br:11449/196619Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:00:37.843346Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar) |
title |
Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar) |
spellingShingle |
Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar) Moreira, Stella H. [UNESP] Picrosirius Low-level laser therapy Skin graft COX-2 CD31 Histology |
title_short |
Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar) |
title_full |
Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar) |
title_fullStr |
Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar) |
title_full_unstemmed |
Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar) |
title_sort |
Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar) |
author |
Moreira, Stella H. [UNESP] |
author_facet |
Moreira, Stella H. [UNESP] Pazzini, Josiane M. Alvarez, Jorge L. G. [UNESP] Cassino, Pedro C. [UNESP] Bustamante, Caio C. [UNESP] Bernardes, Filippo J. L. [UNESP] Kajiura, Caroline Y. [UNESP] De Nardi, Andrigo B. [UNESP] |
author_role |
author |
author2 |
Pazzini, Josiane M. Alvarez, Jorge L. G. [UNESP] Cassino, Pedro C. [UNESP] Bustamante, Caio C. [UNESP] Bernardes, Filippo J. L. [UNESP] Kajiura, Caroline Y. [UNESP] De Nardi, Andrigo B. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Hosp Canc Barretos |
dc.contributor.author.fl_str_mv |
Moreira, Stella H. [UNESP] Pazzini, Josiane M. Alvarez, Jorge L. G. [UNESP] Cassino, Pedro C. [UNESP] Bustamante, Caio C. [UNESP] Bernardes, Filippo J. L. [UNESP] Kajiura, Caroline Y. [UNESP] De Nardi, Andrigo B. [UNESP] |
dc.subject.por.fl_str_mv |
Picrosirius Low-level laser therapy Skin graft COX-2 CD31 Histology |
topic |
Picrosirius Low-level laser therapy Skin graft COX-2 CD31 Histology |
description |
The reconstructive techniques have been widely used in Veterinary Medicine. The post-operative adjuvants therapies like the low-level laser therapy (LLLT) are used to decrease inherent complications to reconstructive surgeries. This article purposed to define the LLLT effects on the healing, inflammation, and vascularization of the skin grafts in applicable time intervals to veterinary surgical routine. Forty rats (Rattus norvegicus albinus wistar) were used and each one was submitted to autogenous cutaneous mesh grafting in the interescapular region. The rats were randomly distributed in five groups (G1, G2, G3, G4, and G5) in accordance with the 6 J/cm(2) or 10 J/cm(2) dose every 3 or 5 days. These treatments were applied on the skin graft for 15 days. The histochemical evaluation with Picrosirius showed greater expression of collagen type 1 - red in grafts of G5 (p < 0.05), while in G1 did not; the expression of collagen type III - green was not induced by LLLT. The histochemical evaluation with hematoxylin-eosin showed greater numbers of fibroblasts in grafts of G4 (p < 0.05) and less hemorrhage in grafts of G5 (p < 0.05). There was modulation of the inflammatory response in irradiated skin grafts. It is concluded the exhibition of the skin grafts to 6 J/cm(2) or 10 J/cm(2) dose every 5 days improved the healing and the modulation of the local inflammation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T19:50:40Z 2020-12-10T19:50:40Z 2020-02-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10103-019-02917-y Lasers In Medical Science. London: Springer London Ltd, v. 35, n. 5, p. 1103-1109, 2020. 0268-8921 http://hdl.handle.net/11449/196619 10.1007/s10103-019-02917-y WOS:000516151100001 |
url |
http://dx.doi.org/10.1007/s10103-019-02917-y http://hdl.handle.net/11449/196619 |
identifier_str_mv |
Lasers In Medical Science. London: Springer London Ltd, v. 35, n. 5, p. 1103-1109, 2020. 0268-8921 10.1007/s10103-019-02917-y WOS:000516151100001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers In Medical Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1103-1109 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129011688144896 |