mRNA profile of Nellore calves after primary infection with Haemonchus placei

Detalhes bibliográficos
Autor(a) principal: Ibelli, A. M. G.
Data de Publicação: 2011
Outros Autores: Nakata, L. C., Andreo, R. [UNESP], Coutinho, L. L., Oliveira, M. C. S., Amarante, Alessandro Francisco Talamini do [UNESP], Furlong, J., Zaros, L. G., Regitano, L. C. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.vetpar.2010.11.013
http://hdl.handle.net/11449/18979
Resumo: Haemonchus parasites are responsible for many losses in animal production. However, few studies are available, especially of zebu cattle. In this study, we investigated mRNA differences of immune response genes in naive Nellore calves infected with Haemonchus placei, relating these differences to patterns of cellular infiltrate. Calves were infected with 15,000 H. placei 13 larvae and after 7 days lymph node and abomasum tissues were collected. IL-2, IL-4, IL-8, IL-12, IL-13, IFN-gamma, MCP-1, lysozyme, pepsinogen and TNF-alpha genes were evaluated by qPCR. Mast cells, eosinophils and globular leukocytes were counted by abomasum histology. In the infected group, IL-4, IL-13 and TNF-alpha were up-regulated in the abomasal lymph node. In the abomasum, IL-13 increased and TNF-alpha was down-regulated (p < 0.05). No differences were detected for mast cells and eosinophil counts in abomasal tissue (p > 0.05). We conclude that for this infection time, there was Th2 polarization but that cellular infiltrate in abomasal tissue takes longer to develop. (C) 2010 Elsevier B.V. All rights reserved.
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spelling mRNA profile of Nellore calves after primary infection with Haemonchus placeiCytokinesBeef cattleHaemonchus placeiBos indicusGastrointestinal parasitesHaemonchus parasites are responsible for many losses in animal production. However, few studies are available, especially of zebu cattle. In this study, we investigated mRNA differences of immune response genes in naive Nellore calves infected with Haemonchus placei, relating these differences to patterns of cellular infiltrate. Calves were infected with 15,000 H. placei 13 larvae and after 7 days lymph node and abomasum tissues were collected. IL-2, IL-4, IL-8, IL-12, IL-13, IFN-gamma, MCP-1, lysozyme, pepsinogen and TNF-alpha genes were evaluated by qPCR. Mast cells, eosinophils and globular leukocytes were counted by abomasum histology. In the infected group, IL-4, IL-13 and TNF-alpha were up-regulated in the abomasal lymph node. In the abomasum, IL-13 increased and TNF-alpha was down-regulated (p < 0.05). No differences were detected for mast cells and eosinophil counts in abomasal tissue (p > 0.05). We conclude that for this infection time, there was Th2 polarization but that cellular infiltrate in abomasal tissue takes longer to develop. (C) 2010 Elsevier B.V. All rights reserved.ProdetabEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Carlos (UFSCar), BR-13565905 São Carlos, SP, BrazilUniv Estadual Paulista, Inst Quim, BR-14801970 Araraquara, SP, BrazilUniv São Paulo, Escola Super Agr Luiz de Queiroz, Dept Zootecnia, BR-13418900 Piracicaba, SP, BrazilEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Pecuaria Sudeste, BR-13560970 São Carlos, SP, BrazilUniv Estadual Paulista, Dept Parasitol, BR-18618000 Botucatu, SP, BrazilEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Gado Leite, BR-36038330 Juiz de Fora, MG, BrazilUniversidade Federal do Rio Grande do Norte (UFRN), BR-59072970 Natal, RN, BrazilUniv Estadual Paulista, Inst Quim, BR-14801970 Araraquara, SP, BrazilUniv Estadual Paulista, Dept Parasitol, BR-18618000 Botucatu, SP, BrazilElsevier B.V.Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Federal do Rio Grande do Norte (UFRN)Ibelli, A. M. G.Nakata, L. C.Andreo, R. [UNESP]Coutinho, L. L.Oliveira, M. C. S.Amarante, Alessandro Francisco Talamini do [UNESP]Furlong, J.Zaros, L. G.Regitano, L. C. A.2014-05-20T13:53:12Z2014-05-20T13:53:12Z2011-03-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article195-200application/pdfhttp://dx.doi.org/10.1016/j.vetpar.2010.11.013Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 176, n. 2-3, p. 195-200, 2011.0304-4017http://hdl.handle.net/11449/1897910.1016/j.vetpar.2010.11.013WOS:000289221400016WOS000289221400016.pdf2677231663329706Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitology2.4221,275info:eu-repo/semantics/openAccess2024-01-27T06:56:33Zoai:repositorio.unesp.br:11449/18979Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:05:44.874410Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv mRNA profile of Nellore calves after primary infection with Haemonchus placei
title mRNA profile of Nellore calves after primary infection with Haemonchus placei
spellingShingle mRNA profile of Nellore calves after primary infection with Haemonchus placei
Ibelli, A. M. G.
Cytokines
Beef cattle
Haemonchus placei
Bos indicus
Gastrointestinal parasites
title_short mRNA profile of Nellore calves after primary infection with Haemonchus placei
title_full mRNA profile of Nellore calves after primary infection with Haemonchus placei
title_fullStr mRNA profile of Nellore calves after primary infection with Haemonchus placei
title_full_unstemmed mRNA profile of Nellore calves after primary infection with Haemonchus placei
title_sort mRNA profile of Nellore calves after primary infection with Haemonchus placei
author Ibelli, A. M. G.
author_facet Ibelli, A. M. G.
Nakata, L. C.
Andreo, R. [UNESP]
Coutinho, L. L.
Oliveira, M. C. S.
Amarante, Alessandro Francisco Talamini do [UNESP]
Furlong, J.
Zaros, L. G.
Regitano, L. C. A.
author_role author
author2 Nakata, L. C.
Andreo, R. [UNESP]
Coutinho, L. L.
Oliveira, M. C. S.
Amarante, Alessandro Francisco Talamini do [UNESP]
Furlong, J.
Zaros, L. G.
Regitano, L. C. A.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Carlos (UFSCar)
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Federal do Rio Grande do Norte (UFRN)
dc.contributor.author.fl_str_mv Ibelli, A. M. G.
Nakata, L. C.
Andreo, R. [UNESP]
Coutinho, L. L.
Oliveira, M. C. S.
Amarante, Alessandro Francisco Talamini do [UNESP]
Furlong, J.
Zaros, L. G.
Regitano, L. C. A.
dc.subject.por.fl_str_mv Cytokines
Beef cattle
Haemonchus placei
Bos indicus
Gastrointestinal parasites
topic Cytokines
Beef cattle
Haemonchus placei
Bos indicus
Gastrointestinal parasites
description Haemonchus parasites are responsible for many losses in animal production. However, few studies are available, especially of zebu cattle. In this study, we investigated mRNA differences of immune response genes in naive Nellore calves infected with Haemonchus placei, relating these differences to patterns of cellular infiltrate. Calves were infected with 15,000 H. placei 13 larvae and after 7 days lymph node and abomasum tissues were collected. IL-2, IL-4, IL-8, IL-12, IL-13, IFN-gamma, MCP-1, lysozyme, pepsinogen and TNF-alpha genes were evaluated by qPCR. Mast cells, eosinophils and globular leukocytes were counted by abomasum histology. In the infected group, IL-4, IL-13 and TNF-alpha were up-regulated in the abomasal lymph node. In the abomasum, IL-13 increased and TNF-alpha was down-regulated (p < 0.05). No differences were detected for mast cells and eosinophil counts in abomasal tissue (p > 0.05). We conclude that for this infection time, there was Th2 polarization but that cellular infiltrate in abomasal tissue takes longer to develop. (C) 2010 Elsevier B.V. All rights reserved.
publishDate 2011
dc.date.none.fl_str_mv 2011-03-10
2014-05-20T13:53:12Z
2014-05-20T13:53:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.vetpar.2010.11.013
Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 176, n. 2-3, p. 195-200, 2011.
0304-4017
http://hdl.handle.net/11449/18979
10.1016/j.vetpar.2010.11.013
WOS:000289221400016
WOS000289221400016.pdf
2677231663329706
url http://dx.doi.org/10.1016/j.vetpar.2010.11.013
http://hdl.handle.net/11449/18979
identifier_str_mv Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 176, n. 2-3, p. 195-200, 2011.
0304-4017
10.1016/j.vetpar.2010.11.013
WOS:000289221400016
WOS000289221400016.pdf
2677231663329706
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Parasitology
2.422
1,275
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 195-200
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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