Phthalocyanine-loaded nanostructured lipid carriers functionalized with folic acid for photodynamic therapy
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.msec.2019.110462 http://hdl.handle.net/11449/199714 |
Resumo: | Breast cancer is a serious public health problem that causes thousands of deaths annually. Chemotherapy continues to play a central role in the management of breast cancer but is associated with extreme off-target toxicity. Therefore, treatments that directly target the tumor and display reduced susceptibility to resistance could improve the outcome and quality of life for patients suffering from this disease. Photodynamic therapy is a targeted treatment based on the use of light to activate a photosensitizer (PS) that then interacts with molecular oxygen and other biochemical substrates to generate cytotoxic levels of Reactive Oxygen Species. Currently approved PS also tends to have poor aqueous solubility that can cause problems when delivered intravenously. In order to circumvent this limitation, in this manuscript, we evaluate the potential of a phthalocyanine-loaded nanostructured lipid carrier (NLC) functionalized with folic acid (FA). To prepare the FA labelled NLC, the polymer PF127 was first esterified with FA and emulsified with an oil phase containing polyoxyethylene 40 stearate, capric/caprylic acid triglycerides, ethoxylated hydrogenated castor oil 40 and the PS zinc phthalocyanine. The resulting PS loaded FA-NLC had a hydrodynamic diameter of 180 nm and were stable in suspension for >90 days. Interestingly, the amount of singlet oxygen generated upon light activation for the PS loaded FA-NLC was substantially higher than the free PS, yet at a lower PS concentration. The PS was released from the NLC in a sustained manner with 4.13 ± 0.58% and 27.7 ± 3.16% after 30 min and 7 days, respectively. Finally, cytotoxicity assays showed that NLC in the concentrations of 09.1 μM of PS present non-toxic with >80 ± 6.8% viable and after 90 s of the light-exposed the results show a statistically significant decrease in cell viability (57 ± 4%). The results obtained allow us to conclude that the functionalized NLC incorporated with PS associated with the PDT technique have characteristics that make them potential candidates for the alternative treatment of breast cancer. |
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Phthalocyanine-loaded nanostructured lipid carriers functionalized with folic acid for photodynamic therapyBreast cancerFolic acidFunctionalizationNanostructured lipid carriersPhotodynamic therapyPhthalocyaninesBreast cancer is a serious public health problem that causes thousands of deaths annually. Chemotherapy continues to play a central role in the management of breast cancer but is associated with extreme off-target toxicity. Therefore, treatments that directly target the tumor and display reduced susceptibility to resistance could improve the outcome and quality of life for patients suffering from this disease. Photodynamic therapy is a targeted treatment based on the use of light to activate a photosensitizer (PS) that then interacts with molecular oxygen and other biochemical substrates to generate cytotoxic levels of Reactive Oxygen Species. Currently approved PS also tends to have poor aqueous solubility that can cause problems when delivered intravenously. In order to circumvent this limitation, in this manuscript, we evaluate the potential of a phthalocyanine-loaded nanostructured lipid carrier (NLC) functionalized with folic acid (FA). To prepare the FA labelled NLC, the polymer PF127 was first esterified with FA and emulsified with an oil phase containing polyoxyethylene 40 stearate, capric/caprylic acid triglycerides, ethoxylated hydrogenated castor oil 40 and the PS zinc phthalocyanine. The resulting PS loaded FA-NLC had a hydrodynamic diameter of 180 nm and were stable in suspension for >90 days. Interestingly, the amount of singlet oxygen generated upon light activation for the PS loaded FA-NLC was substantially higher than the free PS, yet at a lower PS concentration. The PS was released from the NLC in a sustained manner with 4.13 ± 0.58% and 27.7 ± 3.16% after 30 min and 7 days, respectively. Finally, cytotoxicity assays showed that NLC in the concentrations of 09.1 μM of PS present non-toxic with >80 ± 6.8% viable and after 90 s of the light-exposed the results show a statistically significant decrease in cell viability (57 ± 4%). The results obtained allow us to conclude that the functionalized NLC incorporated with PS associated with the PDT technique have characteristics that make them potential candidates for the alternative treatment of breast cancer.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Ulster UniversityConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Graduation Program in Pharmaceutical Sciences Center for Biological and Health Sciences State University of Paraíba (UEPB)São Paulo State University (UNESP) School of Pharmaceutical Sciences Araraquara Department of Drugs and Medicines, Rodovia Araraquara-Jaú, km 1Departamento de Química Universidade Federal de São CarlosSão Paulo State University (UNESP) School of Pharmaceutical Sciences Araraquara Department of Clinical Analyses, Rodovia Araraquara-Jaú, km 1Biomedical Sciences Research Institute University of UlsterSão Paulo State University (UNESP) School of Pharmaceutical Sciences Araraquara Department of Drugs and Medicines, Rodovia Araraquara-Jaú, km 1São Paulo State University (UNESP) School of Pharmaceutical Sciences Araraquara Department of Clinical Analyses, Rodovia Araraquara-Jaú, km 1FAPESP: 2014/50928-2Ulster University: 2016/11198-4Ulster University: 2018/17573-7CNPq: 465687/2014-8State University of Paraíba (UEPB)Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)University of UlsterOshiro-Junior, João A.Sato, Mariana Rillo [UNESP]Boni, Fernanda Isadora [UNESP]Santos, Karen Loraine Macenade Oliveira, Kleber Thiagode Freitas, Laura Marise [UNESP]Fontana, Carla Raquel [UNESP]Nicholas, DeanMcHale, AnthonyCallan, John F.Chorilli, Marlus [UNESP]2020-12-12T01:47:21Z2020-12-12T01:47:21Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.msec.2019.110462Materials Science and Engineering C, v. 108.1873-01910928-4931http://hdl.handle.net/11449/19971410.1016/j.msec.2019.1104622-s2.0-85075568921Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Science and Engineering Cinfo:eu-repo/semantics/openAccess2024-06-24T13:45:38Zoai:repositorio.unesp.br:11449/199714Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:12:58.235343Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Phthalocyanine-loaded nanostructured lipid carriers functionalized with folic acid for photodynamic therapy |
title |
Phthalocyanine-loaded nanostructured lipid carriers functionalized with folic acid for photodynamic therapy |
spellingShingle |
Phthalocyanine-loaded nanostructured lipid carriers functionalized with folic acid for photodynamic therapy Oshiro-Junior, João A. Breast cancer Folic acid Functionalization Nanostructured lipid carriers Photodynamic therapy Phthalocyanines |
title_short |
Phthalocyanine-loaded nanostructured lipid carriers functionalized with folic acid for photodynamic therapy |
title_full |
Phthalocyanine-loaded nanostructured lipid carriers functionalized with folic acid for photodynamic therapy |
title_fullStr |
Phthalocyanine-loaded nanostructured lipid carriers functionalized with folic acid for photodynamic therapy |
title_full_unstemmed |
Phthalocyanine-loaded nanostructured lipid carriers functionalized with folic acid for photodynamic therapy |
title_sort |
Phthalocyanine-loaded nanostructured lipid carriers functionalized with folic acid for photodynamic therapy |
author |
Oshiro-Junior, João A. |
author_facet |
Oshiro-Junior, João A. Sato, Mariana Rillo [UNESP] Boni, Fernanda Isadora [UNESP] Santos, Karen Loraine Macena de Oliveira, Kleber Thiago de Freitas, Laura Marise [UNESP] Fontana, Carla Raquel [UNESP] Nicholas, Dean McHale, Anthony Callan, John F. Chorilli, Marlus [UNESP] |
author_role |
author |
author2 |
Sato, Mariana Rillo [UNESP] Boni, Fernanda Isadora [UNESP] Santos, Karen Loraine Macena de Oliveira, Kleber Thiago de Freitas, Laura Marise [UNESP] Fontana, Carla Raquel [UNESP] Nicholas, Dean McHale, Anthony Callan, John F. Chorilli, Marlus [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
State University of Paraíba (UEPB) Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) University of Ulster |
dc.contributor.author.fl_str_mv |
Oshiro-Junior, João A. Sato, Mariana Rillo [UNESP] Boni, Fernanda Isadora [UNESP] Santos, Karen Loraine Macena de Oliveira, Kleber Thiago de Freitas, Laura Marise [UNESP] Fontana, Carla Raquel [UNESP] Nicholas, Dean McHale, Anthony Callan, John F. Chorilli, Marlus [UNESP] |
dc.subject.por.fl_str_mv |
Breast cancer Folic acid Functionalization Nanostructured lipid carriers Photodynamic therapy Phthalocyanines |
topic |
Breast cancer Folic acid Functionalization Nanostructured lipid carriers Photodynamic therapy Phthalocyanines |
description |
Breast cancer is a serious public health problem that causes thousands of deaths annually. Chemotherapy continues to play a central role in the management of breast cancer but is associated with extreme off-target toxicity. Therefore, treatments that directly target the tumor and display reduced susceptibility to resistance could improve the outcome and quality of life for patients suffering from this disease. Photodynamic therapy is a targeted treatment based on the use of light to activate a photosensitizer (PS) that then interacts with molecular oxygen and other biochemical substrates to generate cytotoxic levels of Reactive Oxygen Species. Currently approved PS also tends to have poor aqueous solubility that can cause problems when delivered intravenously. In order to circumvent this limitation, in this manuscript, we evaluate the potential of a phthalocyanine-loaded nanostructured lipid carrier (NLC) functionalized with folic acid (FA). To prepare the FA labelled NLC, the polymer PF127 was first esterified with FA and emulsified with an oil phase containing polyoxyethylene 40 stearate, capric/caprylic acid triglycerides, ethoxylated hydrogenated castor oil 40 and the PS zinc phthalocyanine. The resulting PS loaded FA-NLC had a hydrodynamic diameter of 180 nm and were stable in suspension for >90 days. Interestingly, the amount of singlet oxygen generated upon light activation for the PS loaded FA-NLC was substantially higher than the free PS, yet at a lower PS concentration. The PS was released from the NLC in a sustained manner with 4.13 ± 0.58% and 27.7 ± 3.16% after 30 min and 7 days, respectively. Finally, cytotoxicity assays showed that NLC in the concentrations of 09.1 μM of PS present non-toxic with >80 ± 6.8% viable and after 90 s of the light-exposed the results show a statistically significant decrease in cell viability (57 ± 4%). The results obtained allow us to conclude that the functionalized NLC incorporated with PS associated with the PDT technique have characteristics that make them potential candidates for the alternative treatment of breast cancer. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:47:21Z 2020-12-12T01:47:21Z 2020-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.msec.2019.110462 Materials Science and Engineering C, v. 108. 1873-0191 0928-4931 http://hdl.handle.net/11449/199714 10.1016/j.msec.2019.110462 2-s2.0-85075568921 |
url |
http://dx.doi.org/10.1016/j.msec.2019.110462 http://hdl.handle.net/11449/199714 |
identifier_str_mv |
Materials Science and Engineering C, v. 108. 1873-0191 0928-4931 10.1016/j.msec.2019.110462 2-s2.0-85075568921 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Materials Science and Engineering C |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128774230769664 |