Avaliação do potencial osteogênico da terapia fotodinâmica em ratas com os principais fatores de risco para a osteonecrose dos maxilares

• Autor(a) principal: Toro, Luan Felipe [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/155205; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-05-08/000880882.pdf
• topic: Osteonecrose; Arcada osseodentária; Difosfonatos; Osso e ossos; Fotoquimioterapia; Osteonecrosis

Bisphosphonates (BPs) are drugs used in the treatment of osteolytic diseases or conditions. Among their adverse effects there is the medication-related osteonecrosis of the jaws (MRONJ). The etiology of the disease is still poorly understood, being one of the reasons why the current preventive and curative treatments do not show high effectiveness. Antimicrobial Photodynamic Therapy (aPDT), due to its powerful biostimulator action and its antimicrobial capacity, is shown as a promising preventive therapeutic strategy for MRONJ. The purpose of this study was to evaluate the osteogenic potential of the aPDT in the site of tooth extraction in female rats presenting the main risk factors to development MRONJ. Thirty female rats (20 months old) were divided in four experimental groups: SAL, SAL/aPDT, ZOL, and ZOL/aPDT. Drug treatment plan lasted seven weeks and 0.45 ml solution of 0.9% NaCl (SAL and SAL/aPDT) or 0.45 ml of this solution plus 100 μg/kg of zoledronate (ZOL and ZOL/aPDT) was administered, every 2 days, by intraperitoneal injection. After three weeks of treatment, extraction of the lower left first molar was performed. The aPDT sessions were performed at 0, 2 and 4 days post extraction in groups SAL/aPDT and ZOL/aPDT. Twenty-eight days after the surgeries all the animals were euthanized. Samples of the site of tooth extraction were processed and submitted to clinical examination, histopathologic and immunohistochemical analysis directed to BMP2/4 (bone morphogenetic protein 2/4), RUNX-2 (runt-related transcription factor 2), OCN (osteocalcin), OPG (osteoprotegerin), RANKL (receptor activator of nuclear factor kappa-β ligand) and TRAP (tartrate-resistant acid phosphatase). In ZOL group the tissue repair process has been severely compromised and consistent with MRONJ. It presented a lower immunostaining...