Karyotype and nucleolus organizer regions of ophisternon aenigmaticum (teleostei: Synbranchiformes: Synbranchidae) from venezuela (in english)
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/231984 |
Resumo: | The first cytogenetic analysis by conventional Giemsa staining, silver nitrate impregnation of nucleolus organizer regions (Ag-NOR), and fluorescence in situ hybridization (FISH) carried out on Ophisternon aenigmaticum from Margarita Island, Venezuela, is reported. Two cytotypes were found: cytotype C1, 2n=46, composed of 6 medium size metacentrics, 2 subtelocentrics and 38 acrocentrics; and cytotype C2, 2n=45, composed of one large metacentric plus 5 medium size metacentrics, 1 subtelocentric and 38 acrocentric chromosomes. In cytotype C1, major ribosomal DNA (rDNA) cistrons are located in the terminal position of the short arms of the first metacentric pair as determined by Ag-NORs and FISH with the 18S rDNA probe. In cytotype C2, silver staining revealed only one positive signal in a single chromosome of the pair 1 whereas FISH with the 18S rDNA probe hybridized in two different chromosomes: One coincident with the Ag-NOR (pair 1) and other in one acrocentric chromosome, suggesting the occurrence of a peri-centric inversion. In both cytotypes, FISH with 5S rDNA probe produced hybridization signals in subtelomeric position of the short arm of the chromosome pair No. 3. Chromosome evolution in Synbranchidae is discussed. |
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Karyotype and nucleolus organizer regions of ophisternon aenigmaticum (teleostei: Synbranchiformes: Synbranchidae) from venezuela (in english)18S DNA5S rDNAChromosome EvolutionChromosome FusionFish ChromosomePericentric InversionThe first cytogenetic analysis by conventional Giemsa staining, silver nitrate impregnation of nucleolus organizer regions (Ag-NOR), and fluorescence in situ hybridization (FISH) carried out on Ophisternon aenigmaticum from Margarita Island, Venezuela, is reported. Two cytotypes were found: cytotype C1, 2n=46, composed of 6 medium size metacentrics, 2 subtelocentrics and 38 acrocentrics; and cytotype C2, 2n=45, composed of one large metacentric plus 5 medium size metacentrics, 1 subtelocentric and 38 acrocentric chromosomes. In cytotype C1, major ribosomal DNA (rDNA) cistrons are located in the terminal position of the short arms of the first metacentric pair as determined by Ag-NORs and FISH with the 18S rDNA probe. In cytotype C2, silver staining revealed only one positive signal in a single chromosome of the pair 1 whereas FISH with the 18S rDNA probe hybridized in two different chromosomes: One coincident with the Ag-NOR (pair 1) and other in one acrocentric chromosome, suggesting the occurrence of a peri-centric inversion. In both cytotypes, FISH with 5S rDNA probe produced hybridization signals in subtelomeric position of the short arm of the chromosome pair No. 3. Chromosome evolution in Synbranchidae is discussed.Universidade Estadual Paulista (UNESP-Botucatu)Universidad de OrienteUNESP-BotucatuUniversidade Estadual de CampinasUniversidade Estadual Paulista Júlio de Mesquita FilhoUniversidade de São PauloUniversidade Estadual Paulista (UNESP-Botucatu)UNESP-BotucatuUniversidade Estadual Paulista Júlio de Mesquita FilhoUniversidade Estadual Paulista (UNESP)Universidad de OrienteUniversidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Nirchio, Mauro [UNESP]Mariguela, Tatiane Casagrande [UNESP]Ferreira, Irani Alves [UNESP]Foresti, Fausto [UNESP]Oliveira, Claudio2022-04-29T08:48:25Z2022-04-29T08:48:25Z2011-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject229-233Interciencia, v. 36, n. 3, p. 229-233, 2011.0378-1844http://hdl.handle.net/11449/2319842-s2.0-79952821652Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengIntercienciainfo:eu-repo/semantics/openAccess2022-04-29T08:48:25Zoai:repositorio.unesp.br:11449/231984Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:34:09.647624Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Karyotype and nucleolus organizer regions of ophisternon aenigmaticum (teleostei: Synbranchiformes: Synbranchidae) from venezuela (in english) |
title |
Karyotype and nucleolus organizer regions of ophisternon aenigmaticum (teleostei: Synbranchiformes: Synbranchidae) from venezuela (in english) |
spellingShingle |
Karyotype and nucleolus organizer regions of ophisternon aenigmaticum (teleostei: Synbranchiformes: Synbranchidae) from venezuela (in english) Nirchio, Mauro [UNESP] 18S DNA 5S rDNA Chromosome Evolution Chromosome Fusion Fish Chromosome Pericentric Inversion |
title_short |
Karyotype and nucleolus organizer regions of ophisternon aenigmaticum (teleostei: Synbranchiformes: Synbranchidae) from venezuela (in english) |
title_full |
Karyotype and nucleolus organizer regions of ophisternon aenigmaticum (teleostei: Synbranchiformes: Synbranchidae) from venezuela (in english) |
title_fullStr |
Karyotype and nucleolus organizer regions of ophisternon aenigmaticum (teleostei: Synbranchiformes: Synbranchidae) from venezuela (in english) |
title_full_unstemmed |
Karyotype and nucleolus organizer regions of ophisternon aenigmaticum (teleostei: Synbranchiformes: Synbranchidae) from venezuela (in english) |
title_sort |
Karyotype and nucleolus organizer regions of ophisternon aenigmaticum (teleostei: Synbranchiformes: Synbranchidae) from venezuela (in english) |
author |
Nirchio, Mauro [UNESP] |
author_facet |
Nirchio, Mauro [UNESP] Mariguela, Tatiane Casagrande [UNESP] Ferreira, Irani Alves [UNESP] Foresti, Fausto [UNESP] Oliveira, Claudio |
author_role |
author |
author2 |
Mariguela, Tatiane Casagrande [UNESP] Ferreira, Irani Alves [UNESP] Foresti, Fausto [UNESP] Oliveira, Claudio |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidad de Oriente Universidade Estadual de Campinas (UNICAMP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Nirchio, Mauro [UNESP] Mariguela, Tatiane Casagrande [UNESP] Ferreira, Irani Alves [UNESP] Foresti, Fausto [UNESP] Oliveira, Claudio |
dc.subject.por.fl_str_mv |
18S DNA 5S rDNA Chromosome Evolution Chromosome Fusion Fish Chromosome Pericentric Inversion |
topic |
18S DNA 5S rDNA Chromosome Evolution Chromosome Fusion Fish Chromosome Pericentric Inversion |
description |
The first cytogenetic analysis by conventional Giemsa staining, silver nitrate impregnation of nucleolus organizer regions (Ag-NOR), and fluorescence in situ hybridization (FISH) carried out on Ophisternon aenigmaticum from Margarita Island, Venezuela, is reported. Two cytotypes were found: cytotype C1, 2n=46, composed of 6 medium size metacentrics, 2 subtelocentrics and 38 acrocentrics; and cytotype C2, 2n=45, composed of one large metacentric plus 5 medium size metacentrics, 1 subtelocentric and 38 acrocentric chromosomes. In cytotype C1, major ribosomal DNA (rDNA) cistrons are located in the terminal position of the short arms of the first metacentric pair as determined by Ag-NORs and FISH with the 18S rDNA probe. In cytotype C2, silver staining revealed only one positive signal in a single chromosome of the pair 1 whereas FISH with the 18S rDNA probe hybridized in two different chromosomes: One coincident with the Ag-NOR (pair 1) and other in one acrocentric chromosome, suggesting the occurrence of a peri-centric inversion. In both cytotypes, FISH with 5S rDNA probe produced hybridization signals in subtelomeric position of the short arm of the chromosome pair No. 3. Chromosome evolution in Synbranchidae is discussed. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-01 2022-04-29T08:48:25Z 2022-04-29T08:48:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Interciencia, v. 36, n. 3, p. 229-233, 2011. 0378-1844 http://hdl.handle.net/11449/231984 2-s2.0-79952821652 |
identifier_str_mv |
Interciencia, v. 36, n. 3, p. 229-233, 2011. 0378-1844 2-s2.0-79952821652 |
url |
http://hdl.handle.net/11449/231984 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Interciencia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
229-233 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129530758430720 |