Genetic diversity of environmental Aspergillus flavus strains in the state of Sao Paulo, Brazil by random amplified polymorphic DNA
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0074-02762007005000102 http://hdl.handle.net/11449/197298 |
Resumo: | Aspergillus flavus is a very important toxigenic fungus that produces aflatoxins, a group of extremely toxic substances to man and animals. Toxigenic fungi can grow in feed crops, such as maize, peanuts, and soybeans, being thus of high concern for public health. There are toxigenic and non-toxigenic A. flavus variants, but the necessary conditions for expressing the toxigenic potential are not fully understood. Therefore, we have studied total-DNA polymorphism from toxigenic and non toxigenic A. flavus strains isolated from maize crops and soil at two geographic locations, 300 km apart, in the Southeast region of Brazil. Total DNA from each A. flavus isolate was extracted and subjected to polymerase chain reaction amplification with five randomic primers through the RAPD (random amplified polymorphic DNA) technique. Phenetic and cladistic analyses of the data, based on bootstrap analyses, led us to conclude that RAPD was not suitable to discriminate toxigenic from non toxigenic strains. But the present results support the use of RAPD for strain characterization, especially for preliminary evaluation over extensive collections. |
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Genetic diversity of environmental Aspergillus flavus strains in the state of Sao Paulo, Brazil by random amplified polymorphic DNAAspergillus flavusrandom amplified polymorphic DNAphylogeny genetic diversityAspergillus flavus is a very important toxigenic fungus that produces aflatoxins, a group of extremely toxic substances to man and animals. Toxigenic fungi can grow in feed crops, such as maize, peanuts, and soybeans, being thus of high concern for public health. There are toxigenic and non-toxigenic A. flavus variants, but the necessary conditions for expressing the toxigenic potential are not fully understood. Therefore, we have studied total-DNA polymorphism from toxigenic and non toxigenic A. flavus strains isolated from maize crops and soil at two geographic locations, 300 km apart, in the Southeast region of Brazil. Total DNA from each A. flavus isolate was extracted and subjected to polymerase chain reaction amplification with five randomic primers through the RAPD (random amplified polymorphic DNA) technique. Phenetic and cladistic analyses of the data, based on bootstrap analyses, led us to conclude that RAPD was not suitable to discriminate toxigenic from non toxigenic strains. But the present results support the use of RAPD for strain characterization, especially for preliminary evaluation over extensive collections.Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo, BrazilUniv Estadual Paulista, Fac Metropolitanas Unit, Sao Paulo, BrazilUniv Santo Amaro, Fac Vet Med, Sao Paulo, BrazilInst Adolfo Lutz Registro, Sao Paulo, BrazilFdn Ezequiel Dias, Belo Horizonte, MG, BrazilUniv Estadual Paulista, Fac Metropolitanas Unit, Sao Paulo, BrazilFundaco Oswaldo CruzInst Ciencias BiomedUniversidade Estadual Paulista (Unesp)Univ Santo AmaroInst Adolfo Lutz RegistroFdn Ezequiel DiasLourenco, AlexandreDurigon, Edison LuisZanotto, PaoloGazzinelli Cruz Madeira, Jovita EugeniaDe Almeida, Adriana PalmaCorrea, Benedito2020-12-10T20:12:32Z2020-12-10T20:12:32Z2007-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article687-692http://dx.doi.org/10.1590/S0074-02762007005000102Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 102, n. 6, p. 687-692, 2007.0074-0276http://hdl.handle.net/11449/19729810.1590/S0074-02762007005000102WOS:000250007400005Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemorias Do Instituto Oswaldo Cruzinfo:eu-repo/semantics/openAccess2024-05-03T13:20:53Zoai:repositorio.unesp.br:11449/197298Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:12:18.012905Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic diversity of environmental Aspergillus flavus strains in the state of Sao Paulo, Brazil by random amplified polymorphic DNA |
title |
Genetic diversity of environmental Aspergillus flavus strains in the state of Sao Paulo, Brazil by random amplified polymorphic DNA |
spellingShingle |
Genetic diversity of environmental Aspergillus flavus strains in the state of Sao Paulo, Brazil by random amplified polymorphic DNA Lourenco, Alexandre Aspergillus flavus random amplified polymorphic DNA phylogeny genetic diversity |
title_short |
Genetic diversity of environmental Aspergillus flavus strains in the state of Sao Paulo, Brazil by random amplified polymorphic DNA |
title_full |
Genetic diversity of environmental Aspergillus flavus strains in the state of Sao Paulo, Brazil by random amplified polymorphic DNA |
title_fullStr |
Genetic diversity of environmental Aspergillus flavus strains in the state of Sao Paulo, Brazil by random amplified polymorphic DNA |
title_full_unstemmed |
Genetic diversity of environmental Aspergillus flavus strains in the state of Sao Paulo, Brazil by random amplified polymorphic DNA |
title_sort |
Genetic diversity of environmental Aspergillus flavus strains in the state of Sao Paulo, Brazil by random amplified polymorphic DNA |
author |
Lourenco, Alexandre |
author_facet |
Lourenco, Alexandre Durigon, Edison Luis Zanotto, Paolo Gazzinelli Cruz Madeira, Jovita Eugenia De Almeida, Adriana Palma Correa, Benedito |
author_role |
author |
author2 |
Durigon, Edison Luis Zanotto, Paolo Gazzinelli Cruz Madeira, Jovita Eugenia De Almeida, Adriana Palma Correa, Benedito |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Inst Ciencias Biomed Universidade Estadual Paulista (Unesp) Univ Santo Amaro Inst Adolfo Lutz Registro Fdn Ezequiel Dias |
dc.contributor.author.fl_str_mv |
Lourenco, Alexandre Durigon, Edison Luis Zanotto, Paolo Gazzinelli Cruz Madeira, Jovita Eugenia De Almeida, Adriana Palma Correa, Benedito |
dc.subject.por.fl_str_mv |
Aspergillus flavus random amplified polymorphic DNA phylogeny genetic diversity |
topic |
Aspergillus flavus random amplified polymorphic DNA phylogeny genetic diversity |
description |
Aspergillus flavus is a very important toxigenic fungus that produces aflatoxins, a group of extremely toxic substances to man and animals. Toxigenic fungi can grow in feed crops, such as maize, peanuts, and soybeans, being thus of high concern for public health. There are toxigenic and non-toxigenic A. flavus variants, but the necessary conditions for expressing the toxigenic potential are not fully understood. Therefore, we have studied total-DNA polymorphism from toxigenic and non toxigenic A. flavus strains isolated from maize crops and soil at two geographic locations, 300 km apart, in the Southeast region of Brazil. Total DNA from each A. flavus isolate was extracted and subjected to polymerase chain reaction amplification with five randomic primers through the RAPD (random amplified polymorphic DNA) technique. Phenetic and cladistic analyses of the data, based on bootstrap analyses, led us to conclude that RAPD was not suitable to discriminate toxigenic from non toxigenic strains. But the present results support the use of RAPD for strain characterization, especially for preliminary evaluation over extensive collections. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-09-01 2020-12-10T20:12:32Z 2020-12-10T20:12:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762007005000102 Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 102, n. 6, p. 687-692, 2007. 0074-0276 http://hdl.handle.net/11449/197298 10.1590/S0074-02762007005000102 WOS:000250007400005 |
url |
http://dx.doi.org/10.1590/S0074-02762007005000102 http://hdl.handle.net/11449/197298 |
identifier_str_mv |
Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 102, n. 6, p. 687-692, 2007. 0074-0276 10.1590/S0074-02762007005000102 WOS:000250007400005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memorias Do Instituto Oswaldo Cruz |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
687-692 |
dc.publisher.none.fl_str_mv |
Fundaco Oswaldo Cruz |
publisher.none.fl_str_mv |
Fundaco Oswaldo Cruz |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129595255291904 |