Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4067/S0717-95022009000100015 http://hdl.handle.net/11449/7008 |
Resumo: | Physical exercise and statins, recommended interventions to dyslipidaemia treatment, are independently related to cardiomyocytes alterations, characterized by miocardic hypertrophy and apoptosis, respectively. Thus, the objective of the present study was to analyze the effects of statin and aerobic physical exercise association in the morphometric parameters of cardiac cell nucleus. 40 male rats adults were divided into four groups: exercised (DE); sedentary (DS), exercised and statin use (DES); sedentary and statin use (DSS). The animals received during the whole experimental period a hiperlipidic diet added 20% of coconut oil and 1.25% of cholesterol; after 30 days of its ingestion, a blood collection was made to verify the dyslipidaemia. Simvastatin (20 mg) was taken five days a week, during eight weeks. During this period, the animals exercised 60 minutes daily in the treadmill. After the last day of the protocol, the cardiac muscle was collected and maintained in liquid nitrogen (-180 degrees C); the cuts were stained by Hematoxilin-Eosin method, and the cardiac fibers were submitted to the nuclear morphometric analyses. The data were analyzed using descriptive analyses, paired T test, Kruskal-Wallis test and Dunn post hoc test; for all analyses, it was adopted p<0.05. It was verified that the group receiving statin presented values statistically significant in comparison to the other groups, in the tridimensional and linear variables. The exercised and statin group, the values obtained in the morphometric analyses were similar to the control group. It is suggested that statins alone can cause alterations in the nucleus of cardiac cells that can be related to apoptosis occurrence and, when exercise is practiced associated to statin administration, the effects of statin can be reduced, what can be related to beneficial adaptations of cardiac mitochondrial in response to physical exercise, turning them more resistant to apoptotic stimuli. |
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Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric StudyEfectos de la ssociación de estatina y del ejercicio aeróbico en los cardiomiocitos de rata. Estudio morfométricoHydroxymethylglutaryl-CoA Reductase InhibitorsExerciseCardiomyocite nucleiPhysical exercise and statins, recommended interventions to dyslipidaemia treatment, are independently related to cardiomyocytes alterations, characterized by miocardic hypertrophy and apoptosis, respectively. Thus, the objective of the present study was to analyze the effects of statin and aerobic physical exercise association in the morphometric parameters of cardiac cell nucleus. 40 male rats adults were divided into four groups: exercised (DE); sedentary (DS), exercised and statin use (DES); sedentary and statin use (DSS). The animals received during the whole experimental period a hiperlipidic diet added 20% of coconut oil and 1.25% of cholesterol; after 30 days of its ingestion, a blood collection was made to verify the dyslipidaemia. Simvastatin (20 mg) was taken five days a week, during eight weeks. During this period, the animals exercised 60 minutes daily in the treadmill. After the last day of the protocol, the cardiac muscle was collected and maintained in liquid nitrogen (-180 degrees C); the cuts were stained by Hematoxilin-Eosin method, and the cardiac fibers were submitted to the nuclear morphometric analyses. The data were analyzed using descriptive analyses, paired T test, Kruskal-Wallis test and Dunn post hoc test; for all analyses, it was adopted p<0.05. It was verified that the group receiving statin presented values statistically significant in comparison to the other groups, in the tridimensional and linear variables. The exercised and statin group, the values obtained in the morphometric analyses were similar to the control group. It is suggested that statins alone can cause alterations in the nucleus of cardiac cells that can be related to apoptosis occurrence and, when exercise is practiced associated to statin administration, the effects of statin can be reduced, what can be related to beneficial adaptations of cardiac mitochondrial in response to physical exercise, turning them more resistant to apoptotic stimuli.El ejercicio físico y las estatinas, intervenciones recomendadas para tratamiento de la dislipidemia, están independientemente relacionadas con las alteraciones de los cardiomiocitos, que se caracterizan por hipertrofia miocárdica y apoptosis, respectivamente. El objetivo del presente estudio fue analizar los efectos de la asociación de estatinas y el ejercicio físico aeróbico en los parámetros morfométricos del núcleo de células cardíacas. 40 ratas macho adultas se dividieron en cuatro grupos: ejercitadas (DE); sedentarias (DS), ejercitadas y con uso de estatina (DES), sedentarias y con uso de estatina (DSS). Los animales recibieron durante todo el período experimental una dieta hiperlipidemica añadiendo 20% de aceite de coco y 1,25% de colesterol. Después de 30 días de su ingestión, se les extrajo sangre para verificar la dislipidemia. Los ejemplares ingirieron Simvastatina (20 mg) cinco días a la semana, durante ocho semanas. Durante este período, los animales ejercitaron 60 minutos diarios en la rueda de andar. Después del último día del protocolo,los animales fueron sacrificados y se les extrajo músculo cardiaco que fue mantenido en nitrógeno líquido (-180°C). De este material se obtuvieron cortes que fueron teñidos por el método de hematoxilina-eosina y las fibras cardiacas fueron sometidas a análisis morfométrico nuclear. Los datos fueron analizados mediante el análisis descriptivo, prueba de la t de Student, prueba de Kruskal-Wallis y Dunn test post hoc. Para todos los análisis fue aprobado p <0,05. Se comprobó que el grupo que recibió estatinas presentó valores estadísticamente significativos en comparación con los otros grupos, en las variables lineales y tridimensionales. En el grupo ejercitado y estatina, los valores obtenidos de los análisis morfométricos fueron similares a los del grupo control. Se sugiere que las estatinas, por sí solas, pueden causar alteraciones en el núcleo de las células cardiacas las cuales pueden estar relacionadas con la ocurrencia de apoptosis y, cuando se practica ejercicio asociado a la administración de estatina, los efectos de las estatinas pueden reducirse, pudiendo resultar beneficioso, en relación con las adaptaciones en la respuesta mitocondrial cardiaca al ejercicio físico, convirtiéndose en más resistentes a los estímulos de apoptosis.São Paulo State Univ, FCT Unesp, Physiotherapy Dept, BR-19060900 Presidente Prudente, SP, BrazilUniv Sao Jose do Rio Preto, Sao Jose do Rio Pre, SP, BrazilSão Paulo State Univ, FCT Unesp, Physiotherapy Dept, BR-19060900 Presidente Prudente, SP, BrazilSoc Chilena AnatomiaUniversidade Estadual Paulista (Unesp)Univ Sao Jose do Rio PretoTrevizan Padulla, Susimary Aparecida [UNESP]Azoubel, ReinaldoBonfim, Mariana RottaAccioly, Marilita FalangolaSilva Camargo Filho, Jose CarlosPadovani, Joao ArmandoBrandao, Antonio CarlosSilva Souza, Doroteia Rossi2014-05-20T13:23:18Z2014-05-20T13:23:18Z2009-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article83-88application/pdfhttp://dx.doi.org/10.4067/S0717-95022009000100015International Journal of Morphology. Temuco: Soc Chilena Anatomia, v. 27, n. 1, p. 83-88, 2009.0717-9502http://hdl.handle.net/11449/7008S0717-95022009000100015WOS:000266571500015S0717-95022009000100015-en.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Morphology0.3360,207info:eu-repo/semantics/openAccess2024-06-18T18:43:33Zoai:repositorio.unesp.br:11449/7008Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:13:06.437582Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study Efectos de la ssociación de estatina y del ejercicio aeróbico en los cardiomiocitos de rata. Estudio morfométrico |
title |
Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study |
spellingShingle |
Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study Trevizan Padulla, Susimary Aparecida [UNESP] Hydroxymethylglutaryl-CoA Reductase Inhibitors Exercise Cardiomyocite nuclei |
title_short |
Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study |
title_full |
Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study |
title_fullStr |
Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study |
title_full_unstemmed |
Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study |
title_sort |
Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study |
author |
Trevizan Padulla, Susimary Aparecida [UNESP] |
author_facet |
Trevizan Padulla, Susimary Aparecida [UNESP] Azoubel, Reinaldo Bonfim, Mariana Rotta Accioly, Marilita Falangola Silva Camargo Filho, Jose Carlos Padovani, Joao Armando Brandao, Antonio Carlos Silva Souza, Doroteia Rossi |
author_role |
author |
author2 |
Azoubel, Reinaldo Bonfim, Mariana Rotta Accioly, Marilita Falangola Silva Camargo Filho, Jose Carlos Padovani, Joao Armando Brandao, Antonio Carlos Silva Souza, Doroteia Rossi |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Sao Jose do Rio Preto |
dc.contributor.author.fl_str_mv |
Trevizan Padulla, Susimary Aparecida [UNESP] Azoubel, Reinaldo Bonfim, Mariana Rotta Accioly, Marilita Falangola Silva Camargo Filho, Jose Carlos Padovani, Joao Armando Brandao, Antonio Carlos Silva Souza, Doroteia Rossi |
dc.subject.por.fl_str_mv |
Hydroxymethylglutaryl-CoA Reductase Inhibitors Exercise Cardiomyocite nuclei |
topic |
Hydroxymethylglutaryl-CoA Reductase Inhibitors Exercise Cardiomyocite nuclei |
description |
Physical exercise and statins, recommended interventions to dyslipidaemia treatment, are independently related to cardiomyocytes alterations, characterized by miocardic hypertrophy and apoptosis, respectively. Thus, the objective of the present study was to analyze the effects of statin and aerobic physical exercise association in the morphometric parameters of cardiac cell nucleus. 40 male rats adults were divided into four groups: exercised (DE); sedentary (DS), exercised and statin use (DES); sedentary and statin use (DSS). The animals received during the whole experimental period a hiperlipidic diet added 20% of coconut oil and 1.25% of cholesterol; after 30 days of its ingestion, a blood collection was made to verify the dyslipidaemia. Simvastatin (20 mg) was taken five days a week, during eight weeks. During this period, the animals exercised 60 minutes daily in the treadmill. After the last day of the protocol, the cardiac muscle was collected and maintained in liquid nitrogen (-180 degrees C); the cuts were stained by Hematoxilin-Eosin method, and the cardiac fibers were submitted to the nuclear morphometric analyses. The data were analyzed using descriptive analyses, paired T test, Kruskal-Wallis test and Dunn post hoc test; for all analyses, it was adopted p<0.05. It was verified that the group receiving statin presented values statistically significant in comparison to the other groups, in the tridimensional and linear variables. The exercised and statin group, the values obtained in the morphometric analyses were similar to the control group. It is suggested that statins alone can cause alterations in the nucleus of cardiac cells that can be related to apoptosis occurrence and, when exercise is practiced associated to statin administration, the effects of statin can be reduced, what can be related to beneficial adaptations of cardiac mitochondrial in response to physical exercise, turning them more resistant to apoptotic stimuli. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-03-01 2014-05-20T13:23:18Z 2014-05-20T13:23:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4067/S0717-95022009000100015 International Journal of Morphology. Temuco: Soc Chilena Anatomia, v. 27, n. 1, p. 83-88, 2009. 0717-9502 http://hdl.handle.net/11449/7008 S0717-95022009000100015 WOS:000266571500015 S0717-95022009000100015-en.pdf |
url |
http://dx.doi.org/10.4067/S0717-95022009000100015 http://hdl.handle.net/11449/7008 |
identifier_str_mv |
International Journal of Morphology. Temuco: Soc Chilena Anatomia, v. 27, n. 1, p. 83-88, 2009. 0717-9502 S0717-95022009000100015 WOS:000266571500015 S0717-95022009000100015-en.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Morphology 0.336 0,207 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
83-88 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Chilena Anatomia |
publisher.none.fl_str_mv |
Soc Chilena Anatomia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128481245003776 |