Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study

Detalhes bibliográficos
Autor(a) principal: Trevizan Padulla, Susimary Aparecida [UNESP]
Data de Publicação: 2009
Outros Autores: Azoubel, Reinaldo, Bonfim, Mariana Rotta, Accioly, Marilita Falangola, Silva Camargo Filho, Jose Carlos, Padovani, Joao Armando, Brandao, Antonio Carlos, Silva Souza, Doroteia Rossi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4067/S0717-95022009000100015
http://hdl.handle.net/11449/7008
Resumo: Physical exercise and statins, recommended interventions to dyslipidaemia treatment, are independently related to cardiomyocytes alterations, characterized by miocardic hypertrophy and apoptosis, respectively. Thus, the objective of the present study was to analyze the effects of statin and aerobic physical exercise association in the morphometric parameters of cardiac cell nucleus. 40 male rats adults were divided into four groups: exercised (DE); sedentary (DS), exercised and statin use (DES); sedentary and statin use (DSS). The animals received during the whole experimental period a hiperlipidic diet added 20% of coconut oil and 1.25% of cholesterol; after 30 days of its ingestion, a blood collection was made to verify the dyslipidaemia. Simvastatin (20 mg) was taken five days a week, during eight weeks. During this period, the animals exercised 60 minutes daily in the treadmill. After the last day of the protocol, the cardiac muscle was collected and maintained in liquid nitrogen (-180 degrees C); the cuts were stained by Hematoxilin-Eosin method, and the cardiac fibers were submitted to the nuclear morphometric analyses. The data were analyzed using descriptive analyses, paired T test, Kruskal-Wallis test and Dunn post hoc test; for all analyses, it was adopted p<0.05. It was verified that the group receiving statin presented values statistically significant in comparison to the other groups, in the tridimensional and linear variables. The exercised and statin group, the values obtained in the morphometric analyses were similar to the control group. It is suggested that statins alone can cause alterations in the nucleus of cardiac cells that can be related to apoptosis occurrence and, when exercise is practiced associated to statin administration, the effects of statin can be reduced, what can be related to beneficial adaptations of cardiac mitochondrial in response to physical exercise, turning them more resistant to apoptotic stimuli.
id UNSP_f85b9d699fe0f1254f3fabe7596b1fe3
oai_identifier_str oai:repositorio.unesp.br:11449/7008
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric StudyEfectos de la ssociación de estatina y del ejercicio aeróbico en los cardiomiocitos de rata. Estudio morfométricoHydroxymethylglutaryl-CoA Reductase InhibitorsExerciseCardiomyocite nucleiPhysical exercise and statins, recommended interventions to dyslipidaemia treatment, are independently related to cardiomyocytes alterations, characterized by miocardic hypertrophy and apoptosis, respectively. Thus, the objective of the present study was to analyze the effects of statin and aerobic physical exercise association in the morphometric parameters of cardiac cell nucleus. 40 male rats adults were divided into four groups: exercised (DE); sedentary (DS), exercised and statin use (DES); sedentary and statin use (DSS). The animals received during the whole experimental period a hiperlipidic diet added 20% of coconut oil and 1.25% of cholesterol; after 30 days of its ingestion, a blood collection was made to verify the dyslipidaemia. Simvastatin (20 mg) was taken five days a week, during eight weeks. During this period, the animals exercised 60 minutes daily in the treadmill. After the last day of the protocol, the cardiac muscle was collected and maintained in liquid nitrogen (-180 degrees C); the cuts were stained by Hematoxilin-Eosin method, and the cardiac fibers were submitted to the nuclear morphometric analyses. The data were analyzed using descriptive analyses, paired T test, Kruskal-Wallis test and Dunn post hoc test; for all analyses, it was adopted p<0.05. It was verified that the group receiving statin presented values statistically significant in comparison to the other groups, in the tridimensional and linear variables. The exercised and statin group, the values obtained in the morphometric analyses were similar to the control group. It is suggested that statins alone can cause alterations in the nucleus of cardiac cells that can be related to apoptosis occurrence and, when exercise is practiced associated to statin administration, the effects of statin can be reduced, what can be related to beneficial adaptations of cardiac mitochondrial in response to physical exercise, turning them more resistant to apoptotic stimuli.El ejercicio físico y las estatinas, intervenciones recomendadas para tratamiento de la dislipidemia, están independientemente relacionadas con las alteraciones de los cardiomiocitos, que se caracterizan por hipertrofia miocárdica y apoptosis, respectivamente. El objetivo del presente estudio fue analizar los efectos de la asociación de estatinas y el ejercicio físico aeróbico en los parámetros morfométricos del núcleo de células cardíacas. 40 ratas macho adultas se dividieron en cuatro grupos: ejercitadas (DE); sedentarias (DS), ejercitadas y con uso de estatina (DES), sedentarias y con uso de estatina (DSS). Los animales recibieron durante todo el período experimental una dieta hiperlipidemica añadiendo 20% de aceite de coco y 1,25% de colesterol. Después de 30 días de su ingestión, se les extrajo sangre para verificar la dislipidemia. Los ejemplares ingirieron Simvastatina (20 mg) cinco días a la semana, durante ocho semanas. Durante este período, los animales ejercitaron 60 minutos diarios en la rueda de andar. Después del último día del protocolo,los animales fueron sacrificados y se les extrajo músculo cardiaco que fue mantenido en nitrógeno líquido (-180°C). De este material se obtuvieron cortes que fueron teñidos por el método de hematoxilina-eosina y las fibras cardiacas fueron sometidas a análisis morfométrico nuclear. Los datos fueron analizados mediante el análisis descriptivo, prueba de la t de Student, prueba de Kruskal-Wallis y Dunn test post hoc. Para todos los análisis fue aprobado p <0,05. Se comprobó que el grupo que recibió estatinas presentó valores estadísticamente significativos en comparación con los otros grupos, en las variables lineales y tridimensionales. En el grupo ejercitado y estatina, los valores obtenidos de los análisis morfométricos fueron similares a los del grupo control. Se sugiere que las estatinas, por sí solas, pueden causar alteraciones en el núcleo de las células cardiacas las cuales pueden estar relacionadas con la ocurrencia de apoptosis y, cuando se practica ejercicio asociado a la administración de estatina, los efectos de las estatinas pueden reducirse, pudiendo resultar beneficioso, en relación con las adaptaciones en la respuesta mitocondrial cardiaca al ejercicio físico, convirtiéndose en más resistentes a los estímulos de apoptosis.São Paulo State Univ, FCT Unesp, Physiotherapy Dept, BR-19060900 Presidente Prudente, SP, BrazilUniv Sao Jose do Rio Preto, Sao Jose do Rio Pre, SP, BrazilSão Paulo State Univ, FCT Unesp, Physiotherapy Dept, BR-19060900 Presidente Prudente, SP, BrazilSoc Chilena AnatomiaUniversidade Estadual Paulista (Unesp)Univ Sao Jose do Rio PretoTrevizan Padulla, Susimary Aparecida [UNESP]Azoubel, ReinaldoBonfim, Mariana RottaAccioly, Marilita FalangolaSilva Camargo Filho, Jose CarlosPadovani, Joao ArmandoBrandao, Antonio CarlosSilva Souza, Doroteia Rossi2014-05-20T13:23:18Z2014-05-20T13:23:18Z2009-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article83-88application/pdfhttp://dx.doi.org/10.4067/S0717-95022009000100015International Journal of Morphology. Temuco: Soc Chilena Anatomia, v. 27, n. 1, p. 83-88, 2009.0717-9502http://hdl.handle.net/11449/7008S0717-95022009000100015WOS:000266571500015S0717-95022009000100015-en.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Morphology0.3360,207info:eu-repo/semantics/openAccess2024-06-18T18:43:33Zoai:repositorio.unesp.br:11449/7008Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:13:06.437582Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study
Efectos de la ssociación de estatina y del ejercicio aeróbico en los cardiomiocitos de rata. Estudio morfométrico
title Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study
spellingShingle Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study
Trevizan Padulla, Susimary Aparecida [UNESP]
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Exercise
Cardiomyocite nuclei
title_short Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study
title_full Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study
title_fullStr Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study
title_full_unstemmed Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study
title_sort Effects of Statin and Aerobic Physical Exercise Association in the Cardiomyocytes of the Rat. Morphometric Study
author Trevizan Padulla, Susimary Aparecida [UNESP]
author_facet Trevizan Padulla, Susimary Aparecida [UNESP]
Azoubel, Reinaldo
Bonfim, Mariana Rotta
Accioly, Marilita Falangola
Silva Camargo Filho, Jose Carlos
Padovani, Joao Armando
Brandao, Antonio Carlos
Silva Souza, Doroteia Rossi
author_role author
author2 Azoubel, Reinaldo
Bonfim, Mariana Rotta
Accioly, Marilita Falangola
Silva Camargo Filho, Jose Carlos
Padovani, Joao Armando
Brandao, Antonio Carlos
Silva Souza, Doroteia Rossi
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ Sao Jose do Rio Preto
dc.contributor.author.fl_str_mv Trevizan Padulla, Susimary Aparecida [UNESP]
Azoubel, Reinaldo
Bonfim, Mariana Rotta
Accioly, Marilita Falangola
Silva Camargo Filho, Jose Carlos
Padovani, Joao Armando
Brandao, Antonio Carlos
Silva Souza, Doroteia Rossi
dc.subject.por.fl_str_mv Hydroxymethylglutaryl-CoA Reductase Inhibitors
Exercise
Cardiomyocite nuclei
topic Hydroxymethylglutaryl-CoA Reductase Inhibitors
Exercise
Cardiomyocite nuclei
description Physical exercise and statins, recommended interventions to dyslipidaemia treatment, are independently related to cardiomyocytes alterations, characterized by miocardic hypertrophy and apoptosis, respectively. Thus, the objective of the present study was to analyze the effects of statin and aerobic physical exercise association in the morphometric parameters of cardiac cell nucleus. 40 male rats adults were divided into four groups: exercised (DE); sedentary (DS), exercised and statin use (DES); sedentary and statin use (DSS). The animals received during the whole experimental period a hiperlipidic diet added 20% of coconut oil and 1.25% of cholesterol; after 30 days of its ingestion, a blood collection was made to verify the dyslipidaemia. Simvastatin (20 mg) was taken five days a week, during eight weeks. During this period, the animals exercised 60 minutes daily in the treadmill. After the last day of the protocol, the cardiac muscle was collected and maintained in liquid nitrogen (-180 degrees C); the cuts were stained by Hematoxilin-Eosin method, and the cardiac fibers were submitted to the nuclear morphometric analyses. The data were analyzed using descriptive analyses, paired T test, Kruskal-Wallis test and Dunn post hoc test; for all analyses, it was adopted p<0.05. It was verified that the group receiving statin presented values statistically significant in comparison to the other groups, in the tridimensional and linear variables. The exercised and statin group, the values obtained in the morphometric analyses were similar to the control group. It is suggested that statins alone can cause alterations in the nucleus of cardiac cells that can be related to apoptosis occurrence and, when exercise is practiced associated to statin administration, the effects of statin can be reduced, what can be related to beneficial adaptations of cardiac mitochondrial in response to physical exercise, turning them more resistant to apoptotic stimuli.
publishDate 2009
dc.date.none.fl_str_mv 2009-03-01
2014-05-20T13:23:18Z
2014-05-20T13:23:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4067/S0717-95022009000100015
International Journal of Morphology. Temuco: Soc Chilena Anatomia, v. 27, n. 1, p. 83-88, 2009.
0717-9502
http://hdl.handle.net/11449/7008
S0717-95022009000100015
WOS:000266571500015
S0717-95022009000100015-en.pdf
url http://dx.doi.org/10.4067/S0717-95022009000100015
http://hdl.handle.net/11449/7008
identifier_str_mv International Journal of Morphology. Temuco: Soc Chilena Anatomia, v. 27, n. 1, p. 83-88, 2009.
0717-9502
S0717-95022009000100015
WOS:000266571500015
S0717-95022009000100015-en.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Morphology
0.336
0,207
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 83-88
application/pdf
dc.publisher.none.fl_str_mv Soc Chilena Anatomia
publisher.none.fl_str_mv Soc Chilena Anatomia
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128481245003776