Retenção de placenta no proteinograma de vacas Holandesas

• Autor(a) principal: Saut, João Paulo Elsen
• Data de Publicação: 2014
• Outros Autores: Miyashiro, Samantha Ive, Raimondo, Raquel Fraga E Silva, Nunes, Mariana Tikuma, Mori, Clara Satsuki, Fagliari, Jose Jurandir, Birgel Junior, Eduardo Harry
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/0103-8478cr20130762; http://hdl.handle.net/11449/114510
• topic: bovinos; puerpério; proteínas de fase aguda; SDS-PAGE; bovine; puerperium; acute phase proteins; SDS-PAGE

The aim of this study was to evaluate the influence of retained placenta on the proteinogram of Holstein cows from commercial dairy farms. Blood samples were collected from 129 animals with retained placenta (RP) and 145 animals with normal delivery and postpartum period, without any treatment, on following days: 1 stday in milk (DIM), 2 nd -3 th , 4 th -5 th , 6 th -7 th , 8 th -14 th , 15 th -29 th , 30 th -59 thand 60 th -90 thDIM were analyzed. Protein electrophoresis were performed in acetate cellulose and sodium dodecyl sulfate polyacrilamide gel (SDS-PAGE), where 19 protein bands were observed with molecular weights between 23KDa and 187KDa. There was no influence on serum total protein and gamma globulins. Serum albumin remained below the normal reference values up to 90DIM. In conclusion, Holstein cows with RP have normoproteinemia with hypoalbuminemia up to 90DIM, presence of positive acute phase response by increase of haptoglobin, ceruloplasmin, acid glycoprotein, alpha and beta globulins and negative acute phase response by decreased of albumin within the first week postpartum.