Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms24054888 http://hdl.handle.net/11449/248508 |
Resumo: | Obesity causes low-grade inflammation that results in the development of comorbidities. In people with obesity, exacerbation of gastric lesion severity and delayed healing may aggravate gastric mucosal lesions. Accordingly, we aimed to evaluate the citral effects on gastric lesion healing in eutrophic and obese animals. C57Bl/6 male mice were divided into two groups: animals fed a standard diet (SD) or high-fat diet (HFD) for 12 weeks. Gastric ulcers were induced using acetic acid (80%) in both groups. Citral (25, 100, or 300 mg/kg) was administered orally for 3 or 10 days. A vehicle-treated negative control (1% Tween 80, 10 mL/kg) and lansoprazole-treated (30 mg/kg) were also established. Lesions were macroscopically examined by quantifying regenerated tissue and ulcer areas. Matrix metalloproteinases (MMP-2 and -9) were analyzed by zymography. The ulcer base area between the two examined periods was significantly reduced in HFD 100 and 300 mg/kg citral-treated animals. In the 100 mg/kg citral-treated group, healing progression was accompanied by reduced MMP-9 activity. Accordingly, HFD could alter MMP-9 activity, delaying the initial healing phase. Although macroscopic changes were undetectable, 10-day treatment with 100 mg/kg citral exhibited improved scar tissue progression in obese animals, with reduced MMP-9 activity and modulation of MMP-2 activation. |
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Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesitycitralgastric ulcerhealingmatrix metalloproteinasesmonoterpeneobesityObesity causes low-grade inflammation that results in the development of comorbidities. In people with obesity, exacerbation of gastric lesion severity and delayed healing may aggravate gastric mucosal lesions. Accordingly, we aimed to evaluate the citral effects on gastric lesion healing in eutrophic and obese animals. C57Bl/6 male mice were divided into two groups: animals fed a standard diet (SD) or high-fat diet (HFD) for 12 weeks. Gastric ulcers were induced using acetic acid (80%) in both groups. Citral (25, 100, or 300 mg/kg) was administered orally for 3 or 10 days. A vehicle-treated negative control (1% Tween 80, 10 mL/kg) and lansoprazole-treated (30 mg/kg) were also established. Lesions were macroscopically examined by quantifying regenerated tissue and ulcer areas. Matrix metalloproteinases (MMP-2 and -9) were analyzed by zymography. The ulcer base area between the two examined periods was significantly reduced in HFD 100 and 300 mg/kg citral-treated animals. In the 100 mg/kg citral-treated group, healing progression was accompanied by reduced MMP-9 activity. Accordingly, HFD could alter MMP-9 activity, delaying the initial healing phase. Although macroscopic changes were undetectable, 10-day treatment with 100 mg/kg citral exhibited improved scar tissue progression in obese animals, with reduced MMP-9 activity and modulation of MMP-2 activation.Department of Structural and Functional Biology (Physiology) Institute of Biosciences of Botucatu São Paulo State University, SPDepartment of Structural and Functional Biology (Physiology) Institute of Biosciences of Botucatu São Paulo State University, SPUniversidade Estadual Paulista (UNESP)Ohara, Rie [UNESP]Dario, Felipe Lima [UNESP]Emílio-Silva, Maycon Tavares [UNESP]Assunção, Renata [UNESP]Rodrigues, Vinícius Peixoto [UNESP]Bueno, Gabriela [UNESP]Raimundo, Priscila Romano [UNESP]da Rocha, Lúcia Regina Machado [UNESP]Hiruma-Lima, Clelia Akiko [UNESP]2023-07-29T13:45:53Z2023-07-29T13:45:53Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ijms24054888International Journal of Molecular Sciences, v. 24, n. 5, 2023.1422-00671661-6596http://hdl.handle.net/11449/24850810.3390/ijms240548882-s2.0-85149889767Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciencesinfo:eu-repo/semantics/openAccess2023-07-29T13:45:53Zoai:repositorio.unesp.br:11449/248508Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:01:26.789119Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity |
title |
Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity |
spellingShingle |
Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity Ohara, Rie [UNESP] citral gastric ulcer healing matrix metalloproteinases monoterpene obesity |
title_short |
Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity |
title_full |
Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity |
title_fullStr |
Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity |
title_full_unstemmed |
Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity |
title_sort |
Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity |
author |
Ohara, Rie [UNESP] |
author_facet |
Ohara, Rie [UNESP] Dario, Felipe Lima [UNESP] Emílio-Silva, Maycon Tavares [UNESP] Assunção, Renata [UNESP] Rodrigues, Vinícius Peixoto [UNESP] Bueno, Gabriela [UNESP] Raimundo, Priscila Romano [UNESP] da Rocha, Lúcia Regina Machado [UNESP] Hiruma-Lima, Clelia Akiko [UNESP] |
author_role |
author |
author2 |
Dario, Felipe Lima [UNESP] Emílio-Silva, Maycon Tavares [UNESP] Assunção, Renata [UNESP] Rodrigues, Vinícius Peixoto [UNESP] Bueno, Gabriela [UNESP] Raimundo, Priscila Romano [UNESP] da Rocha, Lúcia Regina Machado [UNESP] Hiruma-Lima, Clelia Akiko [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Ohara, Rie [UNESP] Dario, Felipe Lima [UNESP] Emílio-Silva, Maycon Tavares [UNESP] Assunção, Renata [UNESP] Rodrigues, Vinícius Peixoto [UNESP] Bueno, Gabriela [UNESP] Raimundo, Priscila Romano [UNESP] da Rocha, Lúcia Regina Machado [UNESP] Hiruma-Lima, Clelia Akiko [UNESP] |
dc.subject.por.fl_str_mv |
citral gastric ulcer healing matrix metalloproteinases monoterpene obesity |
topic |
citral gastric ulcer healing matrix metalloproteinases monoterpene obesity |
description |
Obesity causes low-grade inflammation that results in the development of comorbidities. In people with obesity, exacerbation of gastric lesion severity and delayed healing may aggravate gastric mucosal lesions. Accordingly, we aimed to evaluate the citral effects on gastric lesion healing in eutrophic and obese animals. C57Bl/6 male mice were divided into two groups: animals fed a standard diet (SD) or high-fat diet (HFD) for 12 weeks. Gastric ulcers were induced using acetic acid (80%) in both groups. Citral (25, 100, or 300 mg/kg) was administered orally for 3 or 10 days. A vehicle-treated negative control (1% Tween 80, 10 mL/kg) and lansoprazole-treated (30 mg/kg) were also established. Lesions were macroscopically examined by quantifying regenerated tissue and ulcer areas. Matrix metalloproteinases (MMP-2 and -9) were analyzed by zymography. The ulcer base area between the two examined periods was significantly reduced in HFD 100 and 300 mg/kg citral-treated animals. In the 100 mg/kg citral-treated group, healing progression was accompanied by reduced MMP-9 activity. Accordingly, HFD could alter MMP-9 activity, delaying the initial healing phase. Although macroscopic changes were undetectable, 10-day treatment with 100 mg/kg citral exhibited improved scar tissue progression in obese animals, with reduced MMP-9 activity and modulation of MMP-2 activation. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:45:53Z 2023-07-29T13:45:53Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms24054888 International Journal of Molecular Sciences, v. 24, n. 5, 2023. 1422-0067 1661-6596 http://hdl.handle.net/11449/248508 10.3390/ijms24054888 2-s2.0-85149889767 |
url |
http://dx.doi.org/10.3390/ijms24054888 http://hdl.handle.net/11449/248508 |
identifier_str_mv |
International Journal of Molecular Sciences, v. 24, n. 5, 2023. 1422-0067 1661-6596 10.3390/ijms24054888 2-s2.0-85149889767 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129483039834112 |